[Objective] The experiment aimed to explore the possibility of introducing Elaeagnus angustifolia L. in saline land of West Jilin Province for improving local ecological environment. [Method] The Elaeagnus angustifoli...[Objective] The experiment aimed to explore the possibility of introducing Elaeagnus angustifolia L. in saline land of West Jilin Province for improving local ecological environment. [Method] The Elaeagnus angustifolia L. from Ningxia Province were sown in nursery and practice base of Forestry College in Beihua University to observe their phenophase, growth state, stress resistance and study the relations between survival rate and different cutting medium, grafting methods. [Result] Elaeagnus angustifolia L. could adapt to climate condition in Jilin area and grow well . Among 3 cold-proof measures, there was no freezing injury by soil burial method, although there was 2.8% and 3.9% mortality by covering grassy marshland and winding straw, the cold injury degree was light, so they could go through winter normally. Meanwhile, Elaeagnus angustifolia L. had good saline-alkali tolerance, drought resistance and flood resistance. The successful cutting and graft of Elaeagnus angustifolia L. provided a condition for planting in large-scale and knew the rooting rate of different cutting medium. [Conclusion] Elaeagnus angustifolia L. could be introduced to plant in West Jilin Province.展开更多
The nitrogen （N） distribution and cycling of atmosphere-plant-soil system in the typical meadow Calamagrostis angustifolia wetland （TMCW） and marsh meadow Calamagrostis angustifolia wetland （MMCW） in the Sanjian...The nitrogen （N） distribution and cycling of atmosphere-plant-soil system in the typical meadow Calamagrostis angustifolia wetland （TMCW） and marsh meadow Calamagrostis angustifolia wetland （MMCW） in the Sanjiang plain were studied by a compartment model. The results showed that the N wet deposition amount was 0.757 gN/（m^2·a）, and total inorganic N （TIN） was the main body （0.640 gN/（m^2·a））. The ammonia volatilization amounts of TMCW and MMCW soils in growing season were 0.635 and 0.687 gN/m^2, and the denitrification gaseous lost amounts were 0.617 and 0.405 gN/m^2, respectively. In plant subsystem, the N was mainly stored in root and litter. Soil organic N was the main N storage of the two plant-soil systems and the proportions of it were 93.98% and 92.16%, respectively. The calculation results of N turnovers among compartments of TMCW and MMCW showed that the uptake amounts of root were 23.02 and 28.18 gN/（m^2·a） and the values of aboveground were 11.31 and 6.08 gN/（m^2·a）, the re-translocation amounts from aboveground to root were 5.96 and 2.70 gN/（m^2·a）, the translocation amounts from aboveground living body to litter were 5.35 and 3.38 gN/（m^2·a）, the translocation amounts from litter to soil were larger than 1.55 and 3.01 gN/（m^2·a）, the translocation amounts from root to soil were 14.90 and 13.17 gN/（m^2·a）, and the soil （0-15 cm） N net mineralization amounts were 1.94 and 0.55 gN/（m^2·a）, respectively. The study of N balance indicated that the two plant-soil systems might be situated in the status of lacking N, and the status might induce the degradation of C. angustifolia wetland.展开更多
Deyeuxia angustifolia wetlands were widely distributed in the Sanjiang Plain in Northeast China. Due to strong demand for food production, large-area wetlands were reclaimed to farmlands, which threatened regional eco...Deyeuxia angustifolia wetlands were widely distributed in the Sanjiang Plain in Northeast China. Due to strong demand for food production, large-area wetlands were reclaimed to farmlands, which threatened regional ecological security greatly. Since the 21 th century, returning farmlands to wetlands was widely adopted for natural restoration in the Sangjiang Plain. As the first reflection of wetland restoration, vegetation succession of restored D. angustifolia wetlands should be fully assessed. In this study, vegetation investigation was carried out in three restored D. angustifolia wetlands with 5, 8 and 12 yr restoration, respectively. Meanwhile, a natural D. angustifolia wetland was selected as reference wetland. Results showed that community composition changed greatly and there was visible community succession. Community dominant species changed from composite to gramineae as restoration time increasing.At first, weeds community appeared in the restored wetlands, especially the xerophytes developed to the pioneer species rapidly. And then, mesophytes and wetland species became the dominant species in the restored wetlands. Finally, wetland species, especially D. angustifolia, occupied the dominant position of restored community. Shannon-wiener index(H) and Simpson index(D) both decreased to close to natural D. angustifolia wetlands. Compared with natural D. angustifolia wetland, species composition and diversity in restored wetlands were more complex and higher. As restoration time increasing, there were not significant differences between community characteristics of restored wetlands and natural wetland. All these suggested that vegetation in reclaimed D. angustifolia wetland could be restored naturally, but its restored period is 10 yr at least. From another angle, it is important to protect current natural wetlands.展开更多
The sulfur cycle and its compartmental distribution within an atmosphere-plant-soil system was studied using a compartment model in the typical meadow Calamagrostis angustifolia wetland in the Sanjiang Plain Northeast...The sulfur cycle and its compartmental distribution within an atmosphere-plant-soil system was studied using a compartment model in the typical meadow Calamagrostis angustifolia wetland in the Sanjiang Plain Northeast China. The results showed that in the typical meadow C. angustifolia wetland ecosystem, soil was the main storage compartment and current hinge of sulfur in which 98.4% sulfur was accumulated, while only 1.6% sulfur was accumulated in the plant compartment. In the plant subsystem, roots and litters were the main storage compartment of sulfur and they remained 83.5% of the total plant sulfur. The calculations of sulfur turnover through the compartments of the typical meadow C. angustifolia wetland ecosystem demonstrated that the above-ground component took up 0.99 gS/m^2 from the root, of which 0.16 gS/m^2 was translocated to the roots and 0.83 gS/m^2 to the litter. The roots took in 1.05 gS/m^2 from the soil, subsequent translocation back to the soil accounted for 1.31 gS/m^2, while there was 1.84 gS/m^2 in the litter and the net transfer of sulfur to the soil was more than 0.44 gS/（m^2·a）. The emission of H2S from the typical meadow C. angustifolia wetland ecosystem to the atmosphere was 1.83 mgS/（m^2·a）, while carbonyl sulfide （COS） was absorbed by the typical meadow C. angustifolia wetland ecosystem from the atmosphere at the rate of 1.76 mgS/（m^2·a）. The input of sulfur by the rainfall to the ecosystem was 4.85 mgS/m^2 during the growing season. The difference between input and output was 4.78 mgS/m^2, which indicated that sulfur was accumulated in the ecosystem and may cause wetland acidify in the future.展开更多
In recent years, herbaceous species such as Deyeuxia angustifolia （Kom.） Y. L. Chang has invaded alpine tundra regions of the western slope of the Changbai Mountains. Because atmospheric nitrogen deposition is predi...In recent years, herbaceous species such as Deyeuxia angustifolia （Kom.） Y. L. Chang has invaded alpine tundra regions of the western slope of the Changbai Mountains. Because atmospheric nitrogen deposition is predicted to increase under a warming climate and D. angustifolia is sensitive to nitrogen addition, field experiments were conducted from 2010 to 2013 to determine the effect of increased nitrogen deposition on the mechanisms of D. angustifolia invasion. The goal of this study is to evaluate the impact of increased nitrogen deposition on the changes in alpine tundra vegetation （consisting mostly of Rhododendron chrysanthum Pall. and Vaccinium uliginosum Linn.）. The results showed that： 1） simulated nitrogen deposition affected overall characteristics and structure of R. chrysanthum and E uliginosum communities and had a positive impact on the growth of tundra vegetation invaded by 1）. angustifolia; 2） R. chrysanthum was more resistant to invasion by D. angustifolia than V.. uliginosum; 3） simulated nitrogen deposition could improve the growth and enhance the competitiveness of D. angustifolia, which was gradually replacing R. chrysanthum and V. uliginosum and might become the dominant species in the system in future, transforming alpine tundra into alpine meadow in the Chanebai Mountains.展开更多
Flavonoids identification, total flavonoids content and antioxidant activity of the extracts from the leaves as well as flowers of Cassia angustifolia were determined. Five different extraction techniques were used fo...Flavonoids identification, total flavonoids content and antioxidant activity of the extracts from the leaves as well as flowers of Cassia angustifolia were determined. Five different extraction techniques were used for the extraction of flavonoids from leaves and flowers. Total flavonoid contents (TFC) in the extracts were determined by UV-visible spectrophotometry. Individual flavonoids were identified and quantified by high-performance liquid chromatography coupled with photodiode array detection and electrospray ioniza- tion tandem mass spectrometry (HPLC-PDA-ESI-MS). It has been examined that aqueous ethanol (70%) fractions of C. angustifolia flowers and leaves are highly rich in flavonoids and microwave extraction is the best method for the extraction of individual flavanoid constituents (1 - 9) as well as total flavonoid contents. It was also found that compound 8 was absent in the leaves and compounds 2 and 5 could not be identified. The extracts of C. angustifolia flowers and leaves show strong antioxidant activity.展开更多
A new sesquiterpenoid lactone, 3,6,9-trimethyl-pyrano[2,3,4-de]chromen-2-one (1) and a novel sesquiterpenoid quinone, 6-[2-(5-acetyl-2,7-dimethyl-8-oxo-bicyclo[4.2.0]octa-1,3,5- trien-7-yl)-2-oxo-ethyl]-3,9-dimethyl...A new sesquiterpenoid lactone, 3,6,9-trimethyl-pyrano[2,3,4-de]chromen-2-one (1) and a novel sesquiterpenoid quinone, 6-[2-(5-acetyl-2,7-dimethyl-8-oxo-bicyclo[4.2.0]octa-1,3,5- trien-7-yl)-2-oxo-ethyl]-3,9-dimethylnaphtho[1,8-bc]pyran-7,8-dione (2) together with a known perezone (3) were isolated from the roots of Helicteres angustifolia. The structures were elucidated as mainly on the basis of 1D and 2D NMR spectroscopic data.展开更多
Seeds germination and seedling stages were studied on the legume seeds of Vicia angustifolia L. to reveal the effects of drought stress on germination char- acteristics and water absorption characteristics. The result...Seeds germination and seedling stages were studied on the legume seeds of Vicia angustifolia L. to reveal the effects of drought stress on germination char- acteristics and water absorption characteristics. The results showed that the rate of seed water uptake,germination percentage,germination index,vigor index,seedling height,root length and plant water content decreased with the increase of concen- tration of PEG stress. However,seedling and root dry weight and root/shoot ratio ini- tially increased followed by a decrease. The lowest osmotic potential threshold was -0.42 MPa.展开更多
Objective:To determine the antioxidant and hepatoprotective potential of methanol extract of rhizome of Curcuma angustifolia(MECA)against carbon tetrachloride(CCl4)-induced hepatic damage in vitro and in vivo.Methods:...Objective:To determine the antioxidant and hepatoprotective potential of methanol extract of rhizome of Curcuma angustifolia(MECA)against carbon tetrachloride(CCl4)-induced hepatic damage in vitro and in vivo.Methods:DPPH,ABTS and reducing power assays were performed to estimate the antioxidant effect of MECA.In vitro cytotoxicity of MECA against HepG2 cells was evaluated,whereas serum biochemical parameters and levels of antioxidative enzymes were measured in vivo and in vitro.Additionally,histopathological studies were estimated in order to investigate the hepatoprotective efficacy of MECA.Furthermore,GC-MS analysis of the extract was performed to identify the chemical components.Results:MECA exhibited strong antioxidant activity and attenuated CCl4-induced decrease in the viability of HepG2 cells.Additionally,MECA significantly restored the ALT,AST,ALP,TP and albumin level in comparison with the CCl4 group.After pre-treatment with MECA,effects of SOD,CAT and GSH were increased as well as lipid peroxidation amount decreased on CCl4-induced hepatotoxicity in in vitro and in vivo model.Furthermore,histopathological observation confirmed that MECA reduced liver injury induced by CCl4 in rats.GC-MS analysis confirmed the presence of bioactive constituents such as α-tocopherol(12.27%),phytol(7.61%),squalene(3.71%),β-sitosterol(2.19%),eugenol(2.59%),curcumenol(1.20%),β-elemene(1.00%)and eucalyptol(0.89%).Conclusions:MECA contains antioxidant and hepatoprotective constituents such asα-tocopherol,phytol,squalene and eugenol and exerts hepatoprotective effect both in vitro and in vivo.展开更多
A new flavonoid glucoside with a known one was isolated from the leaves of Cassia angustifolia. Based on the spectral analysis, including MS, ^1H NMR, ^13C NMR, DEPT, ^1H-1HCOSY, HMQC, HMBC and NOSEY, their chemical s...A new flavonoid glucoside with a known one was isolated from the leaves of Cassia angustifolia. Based on the spectral analysis, including MS, ^1H NMR, ^13C NMR, DEPT, ^1H-1HCOSY, HMQC, HMBC and NOSEY, their chemical structures were determined as kaempferol-3-O-[（6＇＇＇-O-trans-sinnapoyt）-β-D-glucopyranosyl （1 → 6）]-β-D-glucopyranoside 1 and apigenin-6,8-di-C-glycoside 2.展开更多
Objective Alzheimer＇s disease（AD） is one of the most important neurodegenerative disorders.It is characterized by dementia including def icits in learning and memory.The present study aimed to evaluate the effects ...Objective Alzheimer＇s disease（AD） is one of the most important neurodegenerative disorders.It is characterized by dementia including def icits in learning and memory.The present study aimed to evaluate the effects of aqueous extract of lavender（Lavandula angustifolia） on spatial performance of AD rats.Methods Male Wistar rats were f irst divided into control and AD groups.Rat model of AD was established by intracerebroventricular injection of 10 ？g A？1-42 20 d prior to administration of the lavender extract.Rats in both groups were then introduced to 2 stages of task learning（with an interval of 20 d） in Morris water maze,each followed by one probe test.After the f irst stage of spatial learning,control and AD animals received different doses（50,100 and 200 mg/kg） of the lavender extract.Results In the first stage of experiment,the latency to locate the hidden platform in AD group was significantly higher than that in control group.However,in the second stage of experiment,control and AD rats that received distilled water（vehicle） showed similar performance,indicating that the maze navigation itself could improve the spatial learning of AD animals.Besides,in the second stage of experiment,control and AD rats that received lavender extract administration at different doses（50,100,and 200 mg/kg） spent less time locating the platform（except for the AD rats with 50 mg/kg extract treatment）,as compared with their counterparts with vehicle treatment,respectively.In addition,lavender extract significantly improved the performance of control and AD rats in the probe test,only at the dose of 200 mg/kg,as compared with their counterparts with vehicle treatment.Conclusion The lavender extract can effectively reverse spatial learning def icits in AD rats.展开更多
Chemical investigation of the pollen of Typha angustifolia Linn.has led to the isolation of two new nonacosanetriols,7,8,10- nonacosanetriol(1) and 7,9,10-nonacosanetriol(2).Their structures were elucidated by chemica...Chemical investigation of the pollen of Typha angustifolia Linn.has led to the isolation of two new nonacosanetriols,7,8,10- nonacosanetriol(1) and 7,9,10-nonacosanetriol(2).Their structures were elucidated by chemical reaction and spectral analysis. Compounds 1 and 2 exhibited weak activity of antiplatelet aggregation in vitro.展开更多
In order to obtain qualitative and quantitative characteristics of leaf epidermal micromorphology and mesophyll structure to evaluate the responses ofElaeagnus angustifolia L. to different environmental factors, epide...In order to obtain qualitative and quantitative characteristics of leaf epidermal micromorphology and mesophyll structure to evaluate the responses ofElaeagnus angustifolia L. to different environmental factors, epidermal micromorphology was observed by scanning electron microscopy （SEM）, and mesophyll structure was studied by light microscopy （LM） and transmission electron microscopy （TEM）. Materials were selected from Linze County, Gansu Province （material A） and Qitai County, Xinjiang Uygur Autonomous Region （material B） of China. Results show that lamina thickness was higher in material A, with one layer of epidermal cells in both adaxial and abaxial surfaces, and epidermal cell radial length was significantly longer in the adaxial surface. E. angustifolia leaves are typically bifacial, with a higher ratio of palisade to spongy tissue in material A. The thickness of trichome layer of epidermis was thicker in material A. In contrast, cell wall and cuticular wax of the epidermal cells were thinner in material A than in material B. Chloroplast ultrastructure was different with the approximate spherical chloroplast containing numerous starch grains and osmiophilic granules in ma- terial A, while only the spindly chloroplast contained starch grains in material B. Multiple layers of peltate or stel- late-peltate trichomes occupied both leaf surfaces in material A and the abaxial surface in material B, while the adaxial surface of material B contained few trichomes. Stomata were not observed on the leaf surfaces in materials A and B by SEM because of trichome obstruction. Our results indicate that the leaf structure of E. angustifolia is closely correlated with environmental factors, and the combination of leaf epidermal micromorphology and mesophyll structure afford re- sistance to environmental stress.展开更多
Objective:To investigate the scientific bases for the traditional use of stachytarpheta angustigolia.MEthods:In vitro antibacterial activity of the aqueous and ethanol extract of the plant was investigated using the a...Objective:To investigate the scientific bases for the traditional use of stachytarpheta angustigolia.MEthods:In vitro antibacterial activity of the aqueous and ethanol extract of the plant was investigated using the agar cup plate diffustion method.Results:The ethanol extract of the plant showed antibacterial activity against Escherichia coli,Streptococcus faecalis,Shigella dysenteriae,Staphylococcus aureus(S.aureus),Salmonella sp., pseudomonas aeruginosa and Neisseria gonorthoseae,while the water extract was active against Escherichia coli,Streptococcus faecalis,Shigella dysenteriae,Staphylococcus aureus and Pseudomonas aeruginasa.The ethanol extract exhibited higher antibacterial activity than the water extract,The minimum inhibitory comcentration(MIC) and minimum bactericidal concentration(MBC) of the ethanol extract were0.65g/L and 0.85g/L,respectively.against S.aureus.Treatment of the extract at higher temperature,60℃ increased the sensitivity of the test of ganisms to the plant extract.Phytochemical analysis indicated that the plant possesses tannins,saponins as well as phenols.Concluslon:A scientific basis that the plant possesses antibacterial activity and it could be a probable source of therapeutic agent.展开更多
基金Supported by the National Natural Science Foundation of China(30470129)the Natural Science Foundation of China University of North(the ninth file in 2007)~~
文摘[Objective] The experiment aimed to explore the possibility of introducing Elaeagnus angustifolia L. in saline land of West Jilin Province for improving local ecological environment. [Method] The Elaeagnus angustifolia L. from Ningxia Province were sown in nursery and practice base of Forestry College in Beihua University to observe their phenophase, growth state, stress resistance and study the relations between survival rate and different cutting medium, grafting methods. [Result] Elaeagnus angustifolia L. could adapt to climate condition in Jilin area and grow well . Among 3 cold-proof measures, there was no freezing injury by soil burial method, although there was 2.8% and 3.9% mortality by covering grassy marshland and winding straw, the cold injury degree was light, so they could go through winter normally. Meanwhile, Elaeagnus angustifolia L. had good saline-alkali tolerance, drought resistance and flood resistance. The successful cutting and graft of Elaeagnus angustifolia L. provided a condition for planting in large-scale and knew the rooting rate of different cutting medium. [Conclusion] Elaeagnus angustifolia L. could be introduced to plant in West Jilin Province.
基金Project supported by the Knowledge Innovation Foundation of Chinese Academy of Sciences(KZCX2-YW-309,KZCX3-SW-332)the National Natural Science Foundation of China(No.920211003).
文摘The nitrogen （N） distribution and cycling of atmosphere-plant-soil system in the typical meadow Calamagrostis angustifolia wetland （TMCW） and marsh meadow Calamagrostis angustifolia wetland （MMCW） in the Sanjiang plain were studied by a compartment model. The results showed that the N wet deposition amount was 0.757 gN/（m^2·a）, and total inorganic N （TIN） was the main body （0.640 gN/（m^2·a））. The ammonia volatilization amounts of TMCW and MMCW soils in growing season were 0.635 and 0.687 gN/m^2, and the denitrification gaseous lost amounts were 0.617 and 0.405 gN/m^2, respectively. In plant subsystem, the N was mainly stored in root and litter. Soil organic N was the main N storage of the two plant-soil systems and the proportions of it were 93.98% and 92.16%, respectively. The calculation results of N turnovers among compartments of TMCW and MMCW showed that the uptake amounts of root were 23.02 and 28.18 gN/（m^2·a） and the values of aboveground were 11.31 and 6.08 gN/（m^2·a）, the re-translocation amounts from aboveground to root were 5.96 and 2.70 gN/（m^2·a）, the translocation amounts from aboveground living body to litter were 5.35 and 3.38 gN/（m^2·a）, the translocation amounts from litter to soil were larger than 1.55 and 3.01 gN/（m^2·a）, the translocation amounts from root to soil were 14.90 and 13.17 gN/（m^2·a）, and the soil （0-15 cm） N net mineralization amounts were 1.94 and 0.55 gN/（m^2·a）, respectively. The study of N balance indicated that the two plant-soil systems might be situated in the status of lacking N, and the status might induce the degradation of C. angustifolia wetland.
基金Under the auspices of National Key Research and Development Program of China(No.2016YFC0500403 2017YFC0505901)National Natural Science Foundation of China(No.41871101)
文摘Deyeuxia angustifolia wetlands were widely distributed in the Sanjiang Plain in Northeast China. Due to strong demand for food production, large-area wetlands were reclaimed to farmlands, which threatened regional ecological security greatly. Since the 21 th century, returning farmlands to wetlands was widely adopted for natural restoration in the Sangjiang Plain. As the first reflection of wetland restoration, vegetation succession of restored D. angustifolia wetlands should be fully assessed. In this study, vegetation investigation was carried out in three restored D. angustifolia wetlands with 5, 8 and 12 yr restoration, respectively. Meanwhile, a natural D. angustifolia wetland was selected as reference wetland. Results showed that community composition changed greatly and there was visible community succession. Community dominant species changed from composite to gramineae as restoration time increasing.At first, weeds community appeared in the restored wetlands, especially the xerophytes developed to the pioneer species rapidly. And then, mesophytes and wetland species became the dominant species in the restored wetlands. Finally, wetland species, especially D. angustifolia, occupied the dominant position of restored community. Shannon-wiener index(H) and Simpson index(D) both decreased to close to natural D. angustifolia wetlands. Compared with natural D. angustifolia wetland, species composition and diversity in restored wetlands were more complex and higher. As restoration time increasing, there were not significant differences between community characteristics of restored wetlands and natural wetland. All these suggested that vegetation in reclaimed D. angustifolia wetland could be restored naturally, but its restored period is 10 yr at least. From another angle, it is important to protect current natural wetlands.
文摘The sulfur cycle and its compartmental distribution within an atmosphere-plant-soil system was studied using a compartment model in the typical meadow Calamagrostis angustifolia wetland in the Sanjiang Plain Northeast China. The results showed that in the typical meadow C. angustifolia wetland ecosystem, soil was the main storage compartment and current hinge of sulfur in which 98.4% sulfur was accumulated, while only 1.6% sulfur was accumulated in the plant compartment. In the plant subsystem, roots and litters were the main storage compartment of sulfur and they remained 83.5% of the total plant sulfur. The calculations of sulfur turnover through the compartments of the typical meadow C. angustifolia wetland ecosystem demonstrated that the above-ground component took up 0.99 gS/m^2 from the root, of which 0.16 gS/m^2 was translocated to the roots and 0.83 gS/m^2 to the litter. The roots took in 1.05 gS/m^2 from the soil, subsequent translocation back to the soil accounted for 1.31 gS/m^2, while there was 1.84 gS/m^2 in the litter and the net transfer of sulfur to the soil was more than 0.44 gS/（m^2·a）. The emission of H2S from the typical meadow C. angustifolia wetland ecosystem to the atmosphere was 1.83 mgS/（m^2·a）, while carbonyl sulfide （COS） was absorbed by the typical meadow C. angustifolia wetland ecosystem from the atmosphere at the rate of 1.76 mgS/（m^2·a）. The input of sulfur by the rainfall to the ecosystem was 4.85 mgS/m^2 during the growing season. The difference between input and output was 4.78 mgS/m^2, which indicated that sulfur was accumulated in the ecosystem and may cause wetland acidify in the future.
基金Special Fund of National Seismological Bureau,China(No.201208005)National Natural Science Foundation of China(No.41171072,41101523)
文摘In recent years, herbaceous species such as Deyeuxia angustifolia （Kom.） Y. L. Chang has invaded alpine tundra regions of the western slope of the Changbai Mountains. Because atmospheric nitrogen deposition is predicted to increase under a warming climate and D. angustifolia is sensitive to nitrogen addition, field experiments were conducted from 2010 to 2013 to determine the effect of increased nitrogen deposition on the mechanisms of D. angustifolia invasion. The goal of this study is to evaluate the impact of increased nitrogen deposition on the changes in alpine tundra vegetation （consisting mostly of Rhododendron chrysanthum Pall. and Vaccinium uliginosum Linn.）. The results showed that： 1） simulated nitrogen deposition affected overall characteristics and structure of R. chrysanthum and E uliginosum communities and had a positive impact on the growth of tundra vegetation invaded by 1）. angustifolia; 2） R. chrysanthum was more resistant to invasion by D. angustifolia than V.. uliginosum; 3） simulated nitrogen deposition could improve the growth and enhance the competitiveness of D. angustifolia, which was gradually replacing R. chrysanthum and V. uliginosum and might become the dominant species in the system in future, transforming alpine tundra into alpine meadow in the Chanebai Mountains.
文摘Flavonoids identification, total flavonoids content and antioxidant activity of the extracts from the leaves as well as flowers of Cassia angustifolia were determined. Five different extraction techniques were used for the extraction of flavonoids from leaves and flowers. Total flavonoid contents (TFC) in the extracts were determined by UV-visible spectrophotometry. Individual flavonoids were identified and quantified by high-performance liquid chromatography coupled with photodiode array detection and electrospray ioniza- tion tandem mass spectrometry (HPLC-PDA-ESI-MS). It has been examined that aqueous ethanol (70%) fractions of C. angustifolia flowers and leaves are highly rich in flavonoids and microwave extraction is the best method for the extraction of individual flavanoid constituents (1 - 9) as well as total flavonoid contents. It was also found that compound 8 was absent in the leaves and compounds 2 and 5 could not be identified. The extracts of C. angustifolia flowers and leaves show strong antioxidant activity.
基金supported by the National Natural Science Foundation of China(NO.20002009,20272085,30271601)Guangdong Provincial Natural Science Foundation(No.021770).
文摘A new sesquiterpenoid lactone, 3,6,9-trimethyl-pyrano[2,3,4-de]chromen-2-one (1) and a novel sesquiterpenoid quinone, 6-[2-(5-acetyl-2,7-dimethyl-8-oxo-bicyclo[4.2.0]octa-1,3,5- trien-7-yl)-2-oxo-ethyl]-3,9-dimethylnaphtho[1,8-bc]pyran-7,8-dione (2) together with a known perezone (3) were isolated from the roots of Helicteres angustifolia. The structures were elucidated as mainly on the basis of 1D and 2D NMR spectroscopic data.
文摘Seeds germination and seedling stages were studied on the legume seeds of Vicia angustifolia L. to reveal the effects of drought stress on germination char- acteristics and water absorption characteristics. The results showed that the rate of seed water uptake,germination percentage,germination index,vigor index,seedling height,root length and plant water content decreased with the increase of concen- tration of PEG stress. However,seedling and root dry weight and root/shoot ratio ini- tially increased followed by a decrease. The lowest osmotic potential threshold was -0.42 MPa.
文摘Objective:To determine the antioxidant and hepatoprotective potential of methanol extract of rhizome of Curcuma angustifolia(MECA)against carbon tetrachloride(CCl4)-induced hepatic damage in vitro and in vivo.Methods:DPPH,ABTS and reducing power assays were performed to estimate the antioxidant effect of MECA.In vitro cytotoxicity of MECA against HepG2 cells was evaluated,whereas serum biochemical parameters and levels of antioxidative enzymes were measured in vivo and in vitro.Additionally,histopathological studies were estimated in order to investigate the hepatoprotective efficacy of MECA.Furthermore,GC-MS analysis of the extract was performed to identify the chemical components.Results:MECA exhibited strong antioxidant activity and attenuated CCl4-induced decrease in the viability of HepG2 cells.Additionally,MECA significantly restored the ALT,AST,ALP,TP and albumin level in comparison with the CCl4 group.After pre-treatment with MECA,effects of SOD,CAT and GSH were increased as well as lipid peroxidation amount decreased on CCl4-induced hepatotoxicity in in vitro and in vivo model.Furthermore,histopathological observation confirmed that MECA reduced liver injury induced by CCl4 in rats.GC-MS analysis confirmed the presence of bioactive constituents such as α-tocopherol(12.27%),phytol(7.61%),squalene(3.71%),β-sitosterol(2.19%),eugenol(2.59%),curcumenol(1.20%),β-elemene(1.00%)and eucalyptol(0.89%).Conclusions:MECA contains antioxidant and hepatoprotective constituents such asα-tocopherol,phytol,squalene and eugenol and exerts hepatoprotective effect both in vitro and in vivo.
文摘A new flavonoid glucoside with a known one was isolated from the leaves of Cassia angustifolia. Based on the spectral analysis, including MS, ^1H NMR, ^13C NMR, DEPT, ^1H-1HCOSY, HMQC, HMBC and NOSEY, their chemical structures were determined as kaempferol-3-O-[（6＇＇＇-O-trans-sinnapoyt）-β-D-glucopyranosyl （1 → 6）]-β-D-glucopyranoside 1 and apigenin-6,8-di-C-glycoside 2.
基金supported by Deputy of Research,Kashan University of Medical Sciences,Kashan,I.R. Iran (No. 8835)
文摘Objective Alzheimer＇s disease（AD） is one of the most important neurodegenerative disorders.It is characterized by dementia including def icits in learning and memory.The present study aimed to evaluate the effects of aqueous extract of lavender（Lavandula angustifolia） on spatial performance of AD rats.Methods Male Wistar rats were f irst divided into control and AD groups.Rat model of AD was established by intracerebroventricular injection of 10 ？g A？1-42 20 d prior to administration of the lavender extract.Rats in both groups were then introduced to 2 stages of task learning（with an interval of 20 d） in Morris water maze,each followed by one probe test.After the f irst stage of spatial learning,control and AD animals received different doses（50,100 and 200 mg/kg） of the lavender extract.Results In the first stage of experiment,the latency to locate the hidden platform in AD group was significantly higher than that in control group.However,in the second stage of experiment,control and AD rats that received distilled water（vehicle） showed similar performance,indicating that the maze navigation itself could improve the spatial learning of AD animals.Besides,in the second stage of experiment,control and AD rats that received lavender extract administration at different doses（50,100,and 200 mg/kg） spent less time locating the platform（except for the AD rats with 50 mg/kg extract treatment）,as compared with their counterparts with vehicle treatment,respectively.In addition,lavender extract significantly improved the performance of control and AD rats in the probe test,only at the dose of 200 mg/kg,as compared with their counterparts with vehicle treatment.Conclusion The lavender extract can effectively reverse spatial learning def icits in AD rats.
基金supported by the Major Program of the National Natural Science Foundation of the Higher Education Institutions of Jiangsu Province(No.06KJA36022)"Qinglan Project"scientific and technological innovation team training program of Jiangsu College and University
文摘Chemical investigation of the pollen of Typha angustifolia Linn.has led to the isolation of two new nonacosanetriols,7,8,10- nonacosanetriol(1) and 7,9,10-nonacosanetriol(2).Their structures were elucidated by chemical reaction and spectral analysis. Compounds 1 and 2 exhibited weak activity of antiplatelet aggregation in vitro.
基金supported by the National Natural Science Foundation of China(Grant No.91125029)the State Key Development Program for Basic Research of China(973 Program,Grant No.2013CB429904)
文摘In order to obtain qualitative and quantitative characteristics of leaf epidermal micromorphology and mesophyll structure to evaluate the responses ofElaeagnus angustifolia L. to different environmental factors, epidermal micromorphology was observed by scanning electron microscopy （SEM）, and mesophyll structure was studied by light microscopy （LM） and transmission electron microscopy （TEM）. Materials were selected from Linze County, Gansu Province （material A） and Qitai County, Xinjiang Uygur Autonomous Region （material B） of China. Results show that lamina thickness was higher in material A, with one layer of epidermal cells in both adaxial and abaxial surfaces, and epidermal cell radial length was significantly longer in the adaxial surface. E. angustifolia leaves are typically bifacial, with a higher ratio of palisade to spongy tissue in material A. The thickness of trichome layer of epidermis was thicker in material A. In contrast, cell wall and cuticular wax of the epidermal cells were thinner in material A than in material B. Chloroplast ultrastructure was different with the approximate spherical chloroplast containing numerous starch grains and osmiophilic granules in ma- terial A, while only the spindly chloroplast contained starch grains in material B. Multiple layers of peltate or stel- late-peltate trichomes occupied both leaf surfaces in material A and the abaxial surface in material B, while the adaxial surface of material B contained few trichomes. Stomata were not observed on the leaf surfaces in materials A and B by SEM because of trichome obstruction. Our results indicate that the leaf structure of E. angustifolia is closely correlated with environmental factors, and the combination of leaf epidermal micromorphology and mesophyll structure afford re- sistance to environmental stress.
文摘Objective:To investigate the scientific bases for the traditional use of stachytarpheta angustigolia.MEthods:In vitro antibacterial activity of the aqueous and ethanol extract of the plant was investigated using the agar cup plate diffustion method.Results:The ethanol extract of the plant showed antibacterial activity against Escherichia coli,Streptococcus faecalis,Shigella dysenteriae,Staphylococcus aureus(S.aureus),Salmonella sp., pseudomonas aeruginosa and Neisseria gonorthoseae,while the water extract was active against Escherichia coli,Streptococcus faecalis,Shigella dysenteriae,Staphylococcus aureus and Pseudomonas aeruginasa.The ethanol extract exhibited higher antibacterial activity than the water extract,The minimum inhibitory comcentration(MIC) and minimum bactericidal concentration(MBC) of the ethanol extract were0.65g/L and 0.85g/L,respectively.against S.aureus.Treatment of the extract at higher temperature,60℃ increased the sensitivity of the test of ganisms to the plant extract.Phytochemical analysis indicated that the plant possesses tannins,saponins as well as phenols.Concluslon:A scientific basis that the plant possesses antibacterial activity and it could be a probable source of therapeutic agent.
