OBJECTIVE:To investigate the effect of Anastatica hierochuntica ethanolic(KEE),aqueous(KAE)extracts,and their combination against CCl4-induced hepatotoxicity in rats.METHODS:The HPLC analysis for KEE and KAE was quant...OBJECTIVE:To investigate the effect of Anastatica hierochuntica ethanolic(KEE),aqueous(KAE)extracts,and their combination against CCl4-induced hepatotoxicity in rats.METHODS:The HPLC analysis for KEE and KAE was quantitatively carried out.Biochemical liver markers,antioxidant enzymes,and histopathological alterations were examined then total hepatoprotection potential was calculated.RESULTS:Among 9 identified phenolic compounds(PC)in KEA,sinapic acid was the highest while syringic acid was the highest among 21 identified PC in KAE.Six flavonoids were identified in KEE and two in KAE using HPLC,respectively.Oral administration of KEE,KAE,and KEE+KAE at 250 mg/kg body weight significantly reduced aspartate aminotransferase(AST),alanine aminotransferase(ALT)levels,alkaline phosphatase(ALP),total bilirubin(TBILI),and also attenuated histopathological changes.Additionally,they reduced malondialdehyde(MOD),restored reduced-glutathione(GSH),and enhanced superoxide dismutase(SOD)levels.KEE,KAE,and KEE+KAE protected the liver from CCl4-hepatotoxicity as they mainly attenuating oxidative stress.Total hepatoprotection was about 128.3%,114.5%,and 103.8%for KEE,KAE,and KEE+KAE,respectively.CONCLUSION:Biochemical observations,supplemented by histopathological examination revealed that AH affords extract-depending protection against CCl4-hepatotoxicity.展开更多
Background: Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions, including complicated childbirth. The principal objective of this research was to provide scientific evidence on the...Background: Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions, including complicated childbirth. The principal objective of this research was to provide scientific evidence on the contractile effect of the aqueous and hydroethanolic extracts of the whole plant from A. hierochuntica during labor. Method: Qualitative and quantitative methods helped identify and quantify the phytoconstituents in both extracts. The antioxidant activity was studied using four standard methods (ABTS, DPPH, FRAP, and LPO). Results: The inhibitory power of the extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase A<sub>2</sub> was determined. The contractile effects of the extracts were evaluated using isolated pregnant mice uteri. Steroids, triterpenoids, flavonoids, tannins, coumarins, and reduced compounds were identified in both extracts. The hydroethanolic extract exhibited higher content of hydrolyzable tannins (15.07 ± 0.24 mg tannic acid equivalent/g) than the aqueous decoction (11.8 ± 0.69 mg TAE/g). The extracts demonstrated the ability to scavenge DPPH and ABTS radicals, reduce ferric ions, and inhibit especially lipid peroxidation. No significant difference was noted in the phospholipase inhibition between the extracts and betamethasone. The hydroethanolic extract displayed the most significant anti-lipoxygenase activity with an IC<sub>50</sub> value of 55.82 ± 1.15 μg/mL. The lethal dose (LD<sub>50</sub>) of extracts was estimated to be superior to 2000 mg/kg body weight (bw). The ability of the samples to produce one cm amplitude of contraction at 50% effective concentrations (EC<sub>50</sub>) was 5.33 × 10<sup>−8</sup> ± 0.32 mg/mL for oxytocin, 1.51 ± 0.08 mg/mL for aqueous extract and 3.57 ± 0.61 mg/mL for hydroethanolic extract. Conclusion: These findings could provide evidence for using Anastatica hierochuntica to facilitate childbirth.展开更多
含生草为十字花科植物,是一种维吾尔常用进口药材,主要生长在中东及撒哈拉沙漠的干旱地区。现代药理学研究表明,含生草具有抗炎、镇痛、抗氧化、保肝、护胃、降血糖等作用,其主要化学成分为黄酮类、挥发油类、有机酸类等。作为传统的维...含生草为十字花科植物,是一种维吾尔常用进口药材,主要生长在中东及撒哈拉沙漠的干旱地区。现代药理学研究表明,含生草具有抗炎、镇痛、抗氧化、保肝、护胃、降血糖等作用,其主要化学成分为黄酮类、挥发油类、有机酸类等。作为传统的维吾尔药材,目前国内对其研究较少,为更好地对含生草的临床药效物质基础和作用机制进行阐释,该文利用Web of Science、NSTL、PubMed、维普、万方等数据库,对含生草的化学成分及药理作用相关文献进行检索并归纳总结,以期为含生草的质量控制、临床应用以及研发提供科学基础。展开更多
The objectives of the present study are to evaluate the chromosomal aberrations in somatic and germ cells of alloxanized diabetic rats treated with methanolic extracts of Cleome droserifolia (Sammo), Anastatica hieroc...The objectives of the present study are to evaluate the chromosomal aberrations in somatic and germ cells of alloxanized diabetic rats treated with methanolic extracts of Cleome droserifolia (Sammo), Anastatica hierochuntica (Kafta), Juniperus phoencea (Araar) and Hyphaene thebaica (Doum) as well as their effects on blood glucose, liver and testes total soluble protein, DNA and RNA contents and also nucleases (RNAase and DNAase) activity. The material and Methods: The study was performed in six groups. The first group was the normal healthy control;the second group was rats injected with alloxan (diabetic control). Every one of the other four groups (diabetic rats) was ingested with each of the methanolic extracts of Kafta, Doum, Sammo and Araar (individually). The results: The results of chromosomal analysis showed that, diabetic rats had a highly significant increase of chromosomal aberrations compared to that of normal healthy control. Animals which treated with the four plants methanolic extracts showed significant improvements in the frequencies of chromosomal aberrations. In case of biochemical analysis, blood glucose level was significantly increased but immunoglobulins levels were decreased in diabetic albino rats. Diabetes increased serum total lipids, cholesterol, triglycerides and LDL but serum HDL was decreased. RNA and DNA as well as inhibited the nucleases (RNAase and DNAase) activity of both organs (liver and testes) tissue. The conclusions: It is concluded that diabetes is much harmful in the animal body, whilst the induction of flavonoids extract (by methanol) of Kafta, Somma, Araar and Doum reduced these harmful of diabetes.展开更多
文摘OBJECTIVE:To investigate the effect of Anastatica hierochuntica ethanolic(KEE),aqueous(KAE)extracts,and their combination against CCl4-induced hepatotoxicity in rats.METHODS:The HPLC analysis for KEE and KAE was quantitatively carried out.Biochemical liver markers,antioxidant enzymes,and histopathological alterations were examined then total hepatoprotection potential was calculated.RESULTS:Among 9 identified phenolic compounds(PC)in KEA,sinapic acid was the highest while syringic acid was the highest among 21 identified PC in KAE.Six flavonoids were identified in KEE and two in KAE using HPLC,respectively.Oral administration of KEE,KAE,and KEE+KAE at 250 mg/kg body weight significantly reduced aspartate aminotransferase(AST),alanine aminotransferase(ALT)levels,alkaline phosphatase(ALP),total bilirubin(TBILI),and also attenuated histopathological changes.Additionally,they reduced malondialdehyde(MOD),restored reduced-glutathione(GSH),and enhanced superoxide dismutase(SOD)levels.KEE,KAE,and KEE+KAE protected the liver from CCl4-hepatotoxicity as they mainly attenuating oxidative stress.Total hepatoprotection was about 128.3%,114.5%,and 103.8%for KEE,KAE,and KEE+KAE,respectively.CONCLUSION:Biochemical observations,supplemented by histopathological examination revealed that AH affords extract-depending protection against CCl4-hepatotoxicity.
文摘Background: Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions, including complicated childbirth. The principal objective of this research was to provide scientific evidence on the contractile effect of the aqueous and hydroethanolic extracts of the whole plant from A. hierochuntica during labor. Method: Qualitative and quantitative methods helped identify and quantify the phytoconstituents in both extracts. The antioxidant activity was studied using four standard methods (ABTS, DPPH, FRAP, and LPO). Results: The inhibitory power of the extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase A<sub>2</sub> was determined. The contractile effects of the extracts were evaluated using isolated pregnant mice uteri. Steroids, triterpenoids, flavonoids, tannins, coumarins, and reduced compounds were identified in both extracts. The hydroethanolic extract exhibited higher content of hydrolyzable tannins (15.07 ± 0.24 mg tannic acid equivalent/g) than the aqueous decoction (11.8 ± 0.69 mg TAE/g). The extracts demonstrated the ability to scavenge DPPH and ABTS radicals, reduce ferric ions, and inhibit especially lipid peroxidation. No significant difference was noted in the phospholipase inhibition between the extracts and betamethasone. The hydroethanolic extract displayed the most significant anti-lipoxygenase activity with an IC<sub>50</sub> value of 55.82 ± 1.15 μg/mL. The lethal dose (LD<sub>50</sub>) of extracts was estimated to be superior to 2000 mg/kg body weight (bw). The ability of the samples to produce one cm amplitude of contraction at 50% effective concentrations (EC<sub>50</sub>) was 5.33 × 10<sup>−8</sup> ± 0.32 mg/mL for oxytocin, 1.51 ± 0.08 mg/mL for aqueous extract and 3.57 ± 0.61 mg/mL for hydroethanolic extract. Conclusion: These findings could provide evidence for using Anastatica hierochuntica to facilitate childbirth.
文摘含生草为十字花科植物,是一种维吾尔常用进口药材,主要生长在中东及撒哈拉沙漠的干旱地区。现代药理学研究表明,含生草具有抗炎、镇痛、抗氧化、保肝、护胃、降血糖等作用,其主要化学成分为黄酮类、挥发油类、有机酸类等。作为传统的维吾尔药材,目前国内对其研究较少,为更好地对含生草的临床药效物质基础和作用机制进行阐释,该文利用Web of Science、NSTL、PubMed、维普、万方等数据库,对含生草的化学成分及药理作用相关文献进行检索并归纳总结,以期为含生草的质量控制、临床应用以及研发提供科学基础。
文摘The objectives of the present study are to evaluate the chromosomal aberrations in somatic and germ cells of alloxanized diabetic rats treated with methanolic extracts of Cleome droserifolia (Sammo), Anastatica hierochuntica (Kafta), Juniperus phoencea (Araar) and Hyphaene thebaica (Doum) as well as their effects on blood glucose, liver and testes total soluble protein, DNA and RNA contents and also nucleases (RNAase and DNAase) activity. The material and Methods: The study was performed in six groups. The first group was the normal healthy control;the second group was rats injected with alloxan (diabetic control). Every one of the other four groups (diabetic rats) was ingested with each of the methanolic extracts of Kafta, Doum, Sammo and Araar (individually). The results: The results of chromosomal analysis showed that, diabetic rats had a highly significant increase of chromosomal aberrations compared to that of normal healthy control. Animals which treated with the four plants methanolic extracts showed significant improvements in the frequencies of chromosomal aberrations. In case of biochemical analysis, blood glucose level was significantly increased but immunoglobulins levels were decreased in diabetic albino rats. Diabetes increased serum total lipids, cholesterol, triglycerides and LDL but serum HDL was decreased. RNA and DNA as well as inhibited the nucleases (RNAase and DNAase) activity of both organs (liver and testes) tissue. The conclusions: It is concluded that diabetes is much harmful in the animal body, whilst the induction of flavonoids extract (by methanol) of Kafta, Somma, Araar and Doum reduced these harmful of diabetes.
