Pineapple (Ananas comosuss, var. Smooth cayenne), which is a popular tropical fruit, is propagated vegetatively. Conventional propagation alone does not provide clean and adequate planting material demanded in Ethiopi...Pineapple (Ananas comosuss, var. Smooth cayenne), which is a popular tropical fruit, is propagated vegetatively. Conventional propagation alone does not provide clean and adequate planting material demanded in Ethiopia. Recently, in vitro multiplication has become a promising technique for large-scale production. However, the acclimatization to the external environment procedure impedes the efficiency, which needs carefully optimized acclimatization techniques. We report optimized acclimatization procedures following first- and second-stage hardening methods for in vitro pineapple plantlets. Primarily, Jiffy-7 peat pellet allowed growing plants vigorously and provided above 8% survival rate over soil mix. Nevertheless, in Ethiopia, soil mix is cheaper and locally accessible. The primarily acclimatized plantlets are needed to be hardened further for better establishment and survival in the field. Black polybag and polysleeve pots filled with soil mix were evaluated in the greenhouse. A significant difference was obtained between pots for number of roots and substrate weight. Polybags had higher root number than polysleeves and saved about 27% of substrates per plant, which is a reduction of 25% of total transportation cost. Hence, the soil mix and polybags were found to be preferable over substrates and pots, for subsequent in vitro pineapple acclimatization.展开更多
The biosynthesis of nanoparticles has been proposed as a cost effective and environmental friendly alternative to chemical and physical methods. Plant mediated synthesis of nanoparticles is a green chemistry approach ...The biosynthesis of nanoparticles has been proposed as a cost effective and environmental friendly alternative to chemical and physical methods. Plant mediated synthesis of nanoparticles is a green chemistry approach that intercom nects nanotechnology and plant biotechnology. In the present study, synthesis of silver nanoparticles (AgNPs) or (Green-Silver) has been demonstrated using extracts of Ananas comosus reducing aqueous silver nitrate. The AgNPs were characterized by Ultraviolet-Visible (UV-vis) Spectrometer, Energy Dispersive X-ray Analysis (EDAX), Selected Area Diffraction Pattern (SAED) and High Resolution Transmission Electron Microscopy (HRTEM). TEM micrographs showed spherical particles with an average size of 12 nm. The XRD pattern showed the characteristic Bragg peaks of (111), (200), (220) and (311) facets of the face center cubic (fcc) silver nanoparticles and confirmed that these nanoparticles are crystalline in nature. The different types of antioxidants presented in the pineapple juice synergistically reduce the Ag metal ions, as each antioxidant is unique in terms of its structure and antioxidant function. The re- action process was simple for formation of silver nanoparticles and AgNPs presented in the aqueous medium were quite stable, even up to 4 months of incubation. This work proved the capability of using biomaterial towards the synthesis of silver nanoparticle, by adopting the principles of green chemistry.展开更多
In this study,fucoidans were extracted from the sea cucumber Thelenota ananas(Ta-FUCs)by enzymatic degradation.Four products with molecular weights of 1380.3,524.0,182.4,and 110.3 kDa were obtained,and the Ta-FUC show...In this study,fucoidans were extracted from the sea cucumber Thelenota ananas(Ta-FUCs)by enzymatic degradation.Four products with molecular weights of 1380.3,524.0,182.4,and 110.3 kDa were obtained,and the Ta-FUC showing optimal anti-adipogenic activities was determined.Results of MTT and Oil red O staining analyses showed that the Ta-FUCs inhibited the proliferation and differentiation of 3T3-L1 adipocytes.Futhermore,Ta-FUCs significantly downregulated the key transcriptional factors,such as SREBP-1c,PPARγ,and C/EBPαof adipocytes.The Ta-FUCs also activated Wnt/β-catenin pathway-related genes,such asβ-catenin,LRP5,and FrZ.The Ta-FUCs suppressed lipid accumulation in 3T3-L1 adipocytes possibly by decreasing the expression of genes ACC,FAS,ME,GPAT,DGAT,and PILN,which are important in the synthesis of fatty acids and triglycerides;and by increasing the expression of genes PPARα,CPT-1α,and ACOX,which are crucial in fatty acidβ-oxidation.The anti-adipogenic activities initially increased and then declined with decreasing molecular weight.Among the Ta-FUCs,the 182.4 kDa Ta-FUC exhibited optimal bioactivities.This study reports for the first time that Ta-FUCs can prevent obesity by regulating the differentiation and lipid accumulation of adipocytes.展开更多
Ananas comosus var.bracteatus is an important ornamental plant because of its green/white chimeric leaves.The accumulation of anthocyanin makes the leaf turn to red especially in the marginal part.However,the red fade...Ananas comosus var.bracteatus is an important ornamental plant because of its green/white chimeric leaves.The accumulation of anthocyanin makes the leaf turn to red especially in the marginal part.However,the red fades away in summer and winter.Light intensity is one of the most important factors affecting leaf color along the seasons.In order to understand the effects of light intensity on the growth and coloration of the chimeric leaves,Ananas comosus var.bracteatus was grown under full sunlight,50%shade and 75%shade for 75 days to evaluate the concentration of pigments,the color parameters(values L^(*),a^(*),b^(*))and the morpho-anatomical variations of chimeric leaves.The results showed that a high irradiance was beneficial to keep the chimeric leaves red.However,prolonged exposure to high irradiance caused a damage,some of the leaves wrinkled and even burned.Shading instead decreased the concentration of anthocyanin and increased the concentration of chlorophyll,especially in the white marginal part of the leaves.Numerous chloroplasts were observed in the mesophyll cells of the white marginal part of the chimeric leaves under shading for 75 days.The increase in chlorophyll concentration resulted in a better growth of plants.In order to balance the growth and coloration of the leaves,approximately 50%shade is suggested to be the optimum light irradiance condition for Ananas comosus var.bracteatus in summer.展开更多
Processing pineapple industry produces huge amounts of waste thus contributing to worsen the global environmental problem. Valorising pineapple waste through further processing until it is transformed into valuable pr...Processing pineapple industry produces huge amounts of waste thus contributing to worsen the global environmental problem. Valorising pineapple waste through further processing until it is transformed into valuable products using environmentally friendly techniques is both, a challenge, and an opportunity. The aim of this review is to characterize and highlight the phytochemical constituents of pineapple peel, their biological activity, and to evaluate the current state-of-art for the utilization of pineapple waste from the processing industry for obtaining pharmaceuticals, food, and beverages, biocombustibles, biodegradable fibers, and other different usage. Pineapple residues are rich in many bioactive compounds such as ferulic acid, vitamin A and C as antioxidant, and containing alkaloids, flavonoids, saponins, tannins, cardiac glycoside, steroids, triterpenoids and phytosterols may provide a good source of several beneficial properties, as well as bromelain that showed significant anticancer activity. Also, pineapple processing residues contain important volatile compounds used as aroma enhancing products and have high potential to produce value-added natural essences. Pineapple peels can be used as nonpharmacological therapeutical in the form of processed food and instant drinks;its potent natural antimicrobial properties may be applied for food conservation and as potential leads to discover new drugs to control some infectious microbial. Pineapple waste is a promising source of metabolites for therapeutics, functional foods, and cosmeceutical applications.展开更多
The present study aim was to assess the preventive effects of Ananas comosus juice consumption on the risk factors of obesity in female Wistar rats. 108 rats were tested for 90 days. After randomization, they were sha...The present study aim was to assess the preventive effects of Ananas comosus juice consumption on the risk factors of obesity in female Wistar rats. 108 rats were tested for 90 days. After randomization, they were shared out into six groups including four experimental groups (GTc, GPlp, GEns and GBrS) and two control groups (<img src="Edit_22b6d494-c259-4132-9806-9c1843ee2402.png" width="15" height="3" align="right" alt="" /><img src="Edit_f761aee0-3be8-44aa-ba0a-865dcc4c7881.png" width="80" height="22" alt="" /><span style="font-family:Verdana;">). The control group consumed 1.20 ml of distilled water, the experimental rats received in oral, 1.20 ml of drinks made from the different parts of Ananas comosus fruit. The measurements of morphometric and biochemical parameters were carried out on Day 0 (D0), Day 30 (D30), Day 60 (D60) and Day 90 (D90). The analysis showed that compared to the positive control rats, the consumption of the various drinks slowed down significantly (P < 0.001);the evolution of the morphometric and biochemical parameters likely to induce obesity in experimental rats which have an increase in a protective factor (HDL;P < 0.001). These results indicate that consumption of different parts of Ananas comosus’s juices had preventive effects on risk factors related to obesity. Moreover, the fruit juice treatment has been found to be more effective.</span>展开更多
研究在N、P、K肥基础上增施有机肥、石灰对巴厘菠萝(Ananas comosusr cv.Comte de Paris)产量和品质的影响,通过测定各处理叶绿素含量、干物质累积量以及产量品质等相关指标,分析其影响规律及差异显著性。结果表明,在催花期之前,同时增...研究在N、P、K肥基础上增施有机肥、石灰对巴厘菠萝(Ananas comosusr cv.Comte de Paris)产量和品质的影响,通过测定各处理叶绿素含量、干物质累积量以及产量品质等相关指标,分析其影响规律及差异显著性。结果表明,在催花期之前,同时增施有机肥和石灰处理与只施无机肥(对照)的叶绿素a、叶绿素b和叶绿素总含量均比单独增施有机肥、石灰处理高,且在催花期前后,同时增施有机肥和石灰处理与单独增施石灰处理叶绿素含量差异显著。增施有机肥和石灰对菠萝植株干物质累积的影响在营养生长期表现出与叶绿素含量变化规律相一致的趋势。产量方面,单独增施有机肥和单独增施石灰处理均降低了菠萝产量,而同时增施有机肥和石灰处理产量最高,为91.98 t/hm^2,比对照增产2.11%。品质方面,单独增施有机肥处理的可溶性糖和可滴定酸含量最高,且与其他施肥处理差异显著;单独增施石灰处理的维生素C含量最高;增施有机肥和石灰可提高菠萝果实糖酸比,改善果实风味。综上可知,施用有机肥、石灰处理可以提高菠萝营养品质和风味,但同时施用才可提高菠萝干物质累积量和产量。展开更多
文摘Pineapple (Ananas comosuss, var. Smooth cayenne), which is a popular tropical fruit, is propagated vegetatively. Conventional propagation alone does not provide clean and adequate planting material demanded in Ethiopia. Recently, in vitro multiplication has become a promising technique for large-scale production. However, the acclimatization to the external environment procedure impedes the efficiency, which needs carefully optimized acclimatization techniques. We report optimized acclimatization procedures following first- and second-stage hardening methods for in vitro pineapple plantlets. Primarily, Jiffy-7 peat pellet allowed growing plants vigorously and provided above 8% survival rate over soil mix. Nevertheless, in Ethiopia, soil mix is cheaper and locally accessible. The primarily acclimatized plantlets are needed to be hardened further for better establishment and survival in the field. Black polybag and polysleeve pots filled with soil mix were evaluated in the greenhouse. A significant difference was obtained between pots for number of roots and substrate weight. Polybags had higher root number than polysleeves and saved about 27% of substrates per plant, which is a reduction of 25% of total transportation cost. Hence, the soil mix and polybags were found to be preferable over substrates and pots, for subsequent in vitro pineapple acclimatization.
文摘The biosynthesis of nanoparticles has been proposed as a cost effective and environmental friendly alternative to chemical and physical methods. Plant mediated synthesis of nanoparticles is a green chemistry approach that intercom nects nanotechnology and plant biotechnology. In the present study, synthesis of silver nanoparticles (AgNPs) or (Green-Silver) has been demonstrated using extracts of Ananas comosus reducing aqueous silver nitrate. The AgNPs were characterized by Ultraviolet-Visible (UV-vis) Spectrometer, Energy Dispersive X-ray Analysis (EDAX), Selected Area Diffraction Pattern (SAED) and High Resolution Transmission Electron Microscopy (HRTEM). TEM micrographs showed spherical particles with an average size of 12 nm. The XRD pattern showed the characteristic Bragg peaks of (111), (200), (220) and (311) facets of the face center cubic (fcc) silver nanoparticles and confirmed that these nanoparticles are crystalline in nature. The different types of antioxidants presented in the pineapple juice synergistically reduce the Ag metal ions, as each antioxidant is unique in terms of its structure and antioxidant function. The re- action process was simple for formation of silver nanoparticles and AgNPs presented in the aqueous medium were quite stable, even up to 4 months of incubation. This work proved the capability of using biomaterial towards the synthesis of silver nanoparticle, by adopting the principles of green chemistry.
基金This study was supported by the National Key Research and Development Program of China(No.2018YFC0311203)the Key Research and Development Plan in Shandong Province(No.2016YYSP017).
文摘In this study,fucoidans were extracted from the sea cucumber Thelenota ananas(Ta-FUCs)by enzymatic degradation.Four products with molecular weights of 1380.3,524.0,182.4,and 110.3 kDa were obtained,and the Ta-FUC showing optimal anti-adipogenic activities was determined.Results of MTT and Oil red O staining analyses showed that the Ta-FUCs inhibited the proliferation and differentiation of 3T3-L1 adipocytes.Futhermore,Ta-FUCs significantly downregulated the key transcriptional factors,such as SREBP-1c,PPARγ,and C/EBPαof adipocytes.The Ta-FUCs also activated Wnt/β-catenin pathway-related genes,such asβ-catenin,LRP5,and FrZ.The Ta-FUCs suppressed lipid accumulation in 3T3-L1 adipocytes possibly by decreasing the expression of genes ACC,FAS,ME,GPAT,DGAT,and PILN,which are important in the synthesis of fatty acids and triglycerides;and by increasing the expression of genes PPARα,CPT-1α,and ACOX,which are crucial in fatty acidβ-oxidation.The anti-adipogenic activities initially increased and then declined with decreasing molecular weight.Among the Ta-FUCs,the 182.4 kDa Ta-FUC exhibited optimal bioactivities.This study reports for the first time that Ta-FUCs can prevent obesity by regulating the differentiation and lipid accumulation of adipocytes.
基金This work was funded by the National Natural Science Foundation of China(Grant Nos.31971704,31770743).
文摘Ananas comosus var.bracteatus is an important ornamental plant because of its green/white chimeric leaves.The accumulation of anthocyanin makes the leaf turn to red especially in the marginal part.However,the red fades away in summer and winter.Light intensity is one of the most important factors affecting leaf color along the seasons.In order to understand the effects of light intensity on the growth and coloration of the chimeric leaves,Ananas comosus var.bracteatus was grown under full sunlight,50%shade and 75%shade for 75 days to evaluate the concentration of pigments,the color parameters(values L^(*),a^(*),b^(*))and the morpho-anatomical variations of chimeric leaves.The results showed that a high irradiance was beneficial to keep the chimeric leaves red.However,prolonged exposure to high irradiance caused a damage,some of the leaves wrinkled and even burned.Shading instead decreased the concentration of anthocyanin and increased the concentration of chlorophyll,especially in the white marginal part of the leaves.Numerous chloroplasts were observed in the mesophyll cells of the white marginal part of the chimeric leaves under shading for 75 days.The increase in chlorophyll concentration resulted in a better growth of plants.In order to balance the growth and coloration of the leaves,approximately 50%shade is suggested to be the optimum light irradiance condition for Ananas comosus var.bracteatus in summer.
文摘Processing pineapple industry produces huge amounts of waste thus contributing to worsen the global environmental problem. Valorising pineapple waste through further processing until it is transformed into valuable products using environmentally friendly techniques is both, a challenge, and an opportunity. The aim of this review is to characterize and highlight the phytochemical constituents of pineapple peel, their biological activity, and to evaluate the current state-of-art for the utilization of pineapple waste from the processing industry for obtaining pharmaceuticals, food, and beverages, biocombustibles, biodegradable fibers, and other different usage. Pineapple residues are rich in many bioactive compounds such as ferulic acid, vitamin A and C as antioxidant, and containing alkaloids, flavonoids, saponins, tannins, cardiac glycoside, steroids, triterpenoids and phytosterols may provide a good source of several beneficial properties, as well as bromelain that showed significant anticancer activity. Also, pineapple processing residues contain important volatile compounds used as aroma enhancing products and have high potential to produce value-added natural essences. Pineapple peels can be used as nonpharmacological therapeutical in the form of processed food and instant drinks;its potent natural antimicrobial properties may be applied for food conservation and as potential leads to discover new drugs to control some infectious microbial. Pineapple waste is a promising source of metabolites for therapeutics, functional foods, and cosmeceutical applications.
文摘The present study aim was to assess the preventive effects of Ananas comosus juice consumption on the risk factors of obesity in female Wistar rats. 108 rats were tested for 90 days. After randomization, they were shared out into six groups including four experimental groups (GTc, GPlp, GEns and GBrS) and two control groups (<img src="Edit_22b6d494-c259-4132-9806-9c1843ee2402.png" width="15" height="3" align="right" alt="" /><img src="Edit_f761aee0-3be8-44aa-ba0a-865dcc4c7881.png" width="80" height="22" alt="" /><span style="font-family:Verdana;">). The control group consumed 1.20 ml of distilled water, the experimental rats received in oral, 1.20 ml of drinks made from the different parts of Ananas comosus fruit. The measurements of morphometric and biochemical parameters were carried out on Day 0 (D0), Day 30 (D30), Day 60 (D60) and Day 90 (D90). The analysis showed that compared to the positive control rats, the consumption of the various drinks slowed down significantly (P < 0.001);the evolution of the morphometric and biochemical parameters likely to induce obesity in experimental rats which have an increase in a protective factor (HDL;P < 0.001). These results indicate that consumption of different parts of Ananas comosus’s juices had preventive effects on risk factors related to obesity. Moreover, the fruit juice treatment has been found to be more effective.</span>
