Objective:To investigate the hook effect in the detection of beta2-microglobulin(β2-MG)with different reagents as well as on two fully automated biochemical analyzers and their solutions.Methods:Using immunoturbidime...Objective:To investigate the hook effect in the detection of beta2-microglobulin(β2-MG)with different reagents as well as on two fully automated biochemical analyzers and their solutions.Methods:Using immunoturbidimetric assay forβ2-MG as the research object,β2-MG levels were detected by different concentration gradients ofβ2-microglobulin samples in Liedemann,Mack,and Myriad reagents of three manufacturers on two automatic biochemical analyzers,and the difference of the hook effect was comparatively analyzed.Results:Leadman reagent showed the hook effect on the Beckman AU5800 automated biochemistry analyzer,while both Maccura and Mindray reagents did not show the hook effect.After the experiments,we found the limit value of the pre-zone check of Leadman reagent,and changed the parameters of the instrument,when the limit value of the pre-zone check was reached,the instrument automatically diluted the specimen five times and then detected it again.After changing the parameters of the instrument,the correlation between the three methods of detecting samples of different concentrations was r>0.99.Conclusion:Before selecting the application of immunoturbidimetric reagents,we have to carry out the risk assessment of the hook effect and selectively set the parameters of the pre-zone check based on the highest concentration that may occur in the clinic;for the items that may have the hook effect,we have to selectively set the parameters of the pre-zone check when the ratio of average reaction rate and the immediate reaction rate is at the limit value,and the phenomenon of antigen excess may occur,the instrument will carry out automatic dilution before detection,so as to avoid the issuance of erroneous results of high-value samples due to the hook effect.展开更多
Background:Lipemia,characterized by elevated triglyceride levels in blood samples,is a prevalent preanalytical interferent in clinical hematology.It leads to erroneous measurements of key complete blood count(CBC)para...Background:Lipemia,characterized by elevated triglyceride levels in blood samples,is a prevalent preanalytical interferent in clinical hematology.It leads to erroneous measurements of key complete blood count(CBC)parameters,including falsely elevated hemoglobin(Hgb)and platelet(PLT)counts.These inaccuracies can compromise diagnostic reliability and patient management.Objective:This review systematically evaluates existing correction methods for lipemic interference in CBC analysis,comparing their efficacy,limitations,and applicability in clinical settings.Methods:We analyze saline replacement,formula-based correction,instrument-specific algorithms,and emerging technologies,supported by experimental and clinical validation data.Conclusion:An optimized,context-dependent strategy is proposed,integrating multiple correction approaches based on lipemia severity.Future research directions,including artificial intelligence(AI)-enhanced corrections and standardized protocols,are discussed to advance hematology testing accuracy.展开更多
Non-dispersive infrared （NDIR） and cavity ring-down spectroscopy （CRDS） CO2 analyzers use 12CO2 isotopologue absorption lines and are insensitive to all or part of other CO2-related isotopologues. This may produce...Non-dispersive infrared （NDIR） and cavity ring-down spectroscopy （CRDS） CO2 analyzers use 12CO2 isotopologue absorption lines and are insensitive to all or part of other CO2-related isotopologues. This may produce biases in CO2 mole fraction measurements of a sample if its carbon isotopic composition deviates from that of the standard gases being used. To evaluate and compare the effects of carbon isotopic composition on NDIR and CRDS CO2 analyzers, we prepared three test sample air cylinders with varying carbon isotopic abundances and calibrated them against five standard cylinders with ambient carbon isotopic composition using CRDS and NDIR systems. We found that the CO2 mole fractions of the sample cylinders measured by G1301 （CRDS） were in good agreement with those measured by LoFlo （NDIR）. The CO2 values measured by both instruments were higher than that of a CO2 isotope measured by G2201i （CRDS） analyzer for a test cylinder with depleted carbon isotopic composition δ^13C =-36.828%0, whereas no obvious difference was found for other two test cylinders with 3 δ^13C=-8.630‰ and δ^13C=-15.380‰, respectively. According to the theoretical and experimental results, we concluded that the total CO2 mole fractions of samples with depleted isotopic compositions can be corrected on the basis of their 12CO2 values calibrated by standard gases using LoFlo and G1301 if the fi13C and fi180 values are known. Keywords NDIR and CRDS analyzers, Carbon isotopic effects, CO2 measurements展开更多
Background There is scarce data about comparisons between geriatric assessment tools in patients with aortic stenosis(AS).We aimed to describe the geriatric profile of patients with AS undergoing transcatheter aortic ...Background There is scarce data about comparisons between geriatric assessment tools in patients with aortic stenosis(AS).We aimed to describe the geriatric profile of patients with AS undergoing transcatheter aortic valve implantation(TAVI)and to analyze the ability of different tools for predicting clinical outcomes in this context.Methods This was a single center retrospective registry including patients with AS undergoing TAVI and surviving to hospital discharge.The primary endpoint was all-cause mortality or need for urgent readmission one year after TAVI.Results A total of 377 patients were included(mean age of 80.4 years).Most patients were independent or mildly dependent,with an optimal cognitive status.The proportion of frailty ranged from 17.6%to 49.8%.A total of 20 patients(5.3%)died and 110/377 patients(29.2%)died or were readmitted during follow up.Overall,most components of the geriatric assessment showed an association with clinical outcomes.Disability for instrumental activities showed a significant association with mortality and a strong association with the rate of mortality or readmission.The association between frailty and clinical outcomes was higher for short physical performance battery(SPPB),essential frailty toolset(EFT)and the frailty index based on comprehensive geriatric assessment(IF-VIG)and lower for Fried criteria and FRAIL scale.Conclusions AS patients from this series presented a good physical performance,optimal cognitive status and a reasonably low prevalence of frailty.The best predictive ability was observed for disability for instrumental activities and frailty as measured by the EFT,SPPB and the IF-VIG.展开更多
One of the detection objectives of the Chinese Asteroid Exploration mission is to investigate the space environment near the Main-belt Comet(MBC,Active Asteroid)311P/PANSTARRS.This paper outlines the scientific object...One of the detection objectives of the Chinese Asteroid Exploration mission is to investigate the space environment near the Main-belt Comet(MBC,Active Asteroid)311P/PANSTARRS.This paper outlines the scientific objectives,measurement targets,and measurement requirements for the proposed Gas and Ion Analyzer(GIA).The GIA is designed for in-situ mass spectrometry of neutral gases and low-energy ions,such as hydrogen,carbon,and oxygen,in the vicinity of 311P.Ion sampling techniques are essential for the GIA's Time-of-Flight(TOF)mass analysis capabilities.In this paper,we present an enhanced ion sampling technique through the development of an ion attraction model and an ion source model.The ion attraction model demonstrates that adjusting attraction grid voltage can enhance the detection efficiency of low-energy ions and mitigate the repulsive force of ions during sampling,which is influenced by the satellite's surface positive charging.The ion source model simulates the processes of gas ionization and ion multiplication.Simulation results indicate that the GIA can achieve a lower pressure limit below 10-13Pa and possess a dynamic range exceeding 10~9.These performances ensure the generation of ions with stable and consistent current,which is crucial for high-resolution and broad dynamic range mass spectrometer analysis.Preliminary testing experiments have verified GIA's capability to detect gas compositions such as H2O and N2.In-situ measurements near 311P using GIA are expected to significantly contribute to our understanding of asteroid activity mechanisms,the evolution of the atmospheric and ionized environments of main-belt comets,the interactions with solar wind,and the origin of Earth's water.展开更多
A Linear Feedback Shift Register (LFSR)can be used to compress test response data as a Signature Analyzer(SA). Parallel Signature Analyzers (PSAs) implemented as multiple input LFSRs are faster and re- quire less hard...A Linear Feedback Shift Register (LFSR)can be used to compress test response data as a Signature Analyzer(SA). Parallel Signature Analyzers (PSAs) implemented as multiple input LFSRs are faster and re- quire less hardware overhead than Serial Signature Analyzers (SSAs)for compacting test response data for Built-In Serf-Test (BIST)in IC or hoard-testing environments. However, the SAs are prone to aliasing errors because of some specific types of error patterns. An alias is a faulty output signature that is identical to the fault-free signature. A penetrating analysis of detecting capability of SAs depends strongly on mathematical manipulations, instead of being aware of some special cases or examples. In addition , the analysis should not be restricted to a particular structure of LFSR, but be appropriate for various structures of LFSRs. This pa- per presents necessary and sufficient conditions for aliasing errors based on a complete mathematical descrip- tion of various types of SAs. An LFSR reconfiguration scheme is suggested which will prevent any aliasing double errors. Such a prevention cannot be obtained by any extension of an LFSR.展开更多
The residual biomass composed of pseudo trunks and banana leaves is very important and poorly valued. There is very little quantified data on the deposits of residual biomass from banana plantations in Senegal and in ...The residual biomass composed of pseudo trunks and banana leaves is very important and poorly valued. There is very little quantified data on the deposits of residual biomass from banana plantations in Senegal and in particular in the Tambacounda region. In this work, we seek to evaluate the methanogenic potential and to valorize this biomass in biogas and biofertilizer. The laboratory experiment lasted approximately 35 days. During this time, the methanogenic microorganisms degrade the organic residue provided, which results in the production of biogas. At the end of the reactions, the rate of biogas production drops, indicating the end of the biodegradation of organic matter. Biogas production is measured over time and the composition of the biogas produced is analyzed by gas chromatography (GC) or by an infrared analyzer. The methane potential of each sample is determined from the cumulative quantity of methane produced in each flask representing a digestion system. The measurement can be expressed in m3 of CH4 per tonne of dry matter or per tonne of raw material. The first challenge of this study therefore lies in the acquisition of reliable and usable data to quantify the methanizable biomass. This study will allow us not only to evaluate the quantities of pseudo trunks and banana leaves available in a plot after harvest but also to test the biogas and methane production potential (BMP test) of this substrate and therefore determine the expected biogas production of this biomass.展开更多
The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due ...The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due to the complexity of reagent addition and incubation processes.This study presents a smartphone-integrated handheld automated biochemical analyzer(sHABA)designed for point-of-care testing of urinary albumin.The sHABA features a pre-loaded,disposable reagent cassette with reagents for the albumin assay arranged in the order of their addition within a hose.The smartphone-integrated analyzer can drive the reagents following a preset program,to enable automatic sequential addition.The sHABA has a detection limit for albumin of 5.9 mg/L and a linear detection range from 7 to 450 mg/L.The consistency of albumin level detection in 931 urine samples using sHABA with clinical tests indicates good sensitivity(95.78%)and specificity(90.16%).This research advances the field by providing an automated detection method for albumin in a portable device,allowing even untrained individuals to monitor CKD in real time at the patient's bedside.In the context of promoting tiered diagnosis and treatment,the sHABA has the potential to become an essential tool for the early diagnosis and comprehensive management of CKD and other chronic conditions.展开更多
Biomechanical study of the visual system by ocular response analyzer investigates the inter-structural biological relationships,mechanics,and function of the visual system.This review aimed to investigate the changes ...Biomechanical study of the visual system by ocular response analyzer investigates the inter-structural biological relationships,mechanics,and function of the visual system.This review aimed to investigate the changes in corneal biomechanical parameters with age and sex.The articles published in PubMed between 2000 and 2021 were investigated and critiqued,and valid scientific evidence was collected,reviewed and concluded according to the inclusion and exclusion criteria.Most studies showed that corneal biomechanical changes occur infrequently in children up to the age of 20y,and with increasing age and wider age range,there was a significant decrease in corneal biomechanical indices,especially corneal hysteresis.In children and adults,most studies have shown that these biomechanical indicators,especially corneal resistance factor,were higher in females.Although hormonal changes may contribute to this finding,the role of axial length and other biometric indicators should not be ignored.The axial length,the intraocular pressure,and the corneal thickness are other factors associated with biomechanical parameters that should be taken into account in clinical diagnosis and management especially for patients undergoing refractive surgery as well as keratoconus patients.展开更多
Air-sea water vapor and CO_(2) flux observation experiments were carried out at the Yantai National Satellite Ocean Calibration Platform and the jetty at Monolithic Beach,Juehua Island,using a 100 Hz gas analyzer.The ...Air-sea water vapor and CO_(2) flux observation experiments were carried out at the Yantai National Satellite Ocean Calibration Platform and the jetty at Monolithic Beach,Juehua Island,using a 100 Hz gas analyzer.The observations were corrected by employing wild point rejection,linear detrending,delay correction,coordinate rotation,time matching,and Webb,Pearman,and Leuning(WPL)correction.The results of spectral analysis and a turbulence development adequacy data quality check showed that the overall observation data quality was good.The air-sea water vapor and CO_(2) flux results showed that the observation duration affected both the air-sea flux intensity and direction at different observation frequencies.At shorter observation durations,the air-sea flux values measured at 100 Hz were smaller than the 20 Hz measurements and had opposite directions.In addition,the WPL correction reduced the overall air-sea flux and partially minimized the effect of observation frequency on the air-sea flux intensity.These results showed that high-frequency observations showed more turbulence variations than low-frequency observations.This conclusion could promote an understanding of small-scale turbulence variations.展开更多
Seedlessness has always been a valuable quality characteristic of edible grape varieties.Although the production of seedless grapes has been ongoing for decades,the genetic complexity of seedless grapes is not yet ful...Seedlessness has always been a valuable quality characteristic of edible grape varieties.Although the production of seedless grapes has been ongoing for decades,the genetic complexity of seedless grapes is not yet fully understood.Therefore,determining the genetic mechanisms and key regulatory genes of seedless grapes is of great significance for seedless grape breeding and meeting market demands.The emergence of high-throughput analysis software offers greater possibilities for mining genes related to plant organ development.Specifically,to mine a greater number of candidate genes related to grape seed traits,this study used the seed trait parameters analyzed by Tomato Analyzer as the target trait and then used a genome-wide association study(GWAS)to mine candidate genes.In the two-year analysis using principal component analysis(PCA),we extracted five principal components with a cumulative contribution rate of 96.586%.The cumulative contribution rate for component 1 reached 87.352%.Correlation analysis revealed correlation coefficients ranging from 0.54 to 0.98 among the seven basic traits.The GWAS results indicated that 370 SNP loci were significantly correlated with seed traits.These SNP loci were distributed on 18 chromosomes,except for chromosome 4,with most SNP loci distributed on chromosome 18.Based on the physical location of single nucleotide polymorphism(SNP)markers significantly associated with seed-related traits in the grape reference genome,candidate genes are screened within the range of linkage disequilibrium(LD)attenuation distance,both upstream and downstream of the significant SNP loci.These candidate genes were mainly transcription factor-related genes(VvMADS4 and VvMADS5),ubiquitin ligase-related genes(E3 ubiquitin ligase BIG BROTHER),serine/threonine protein kinase-related genes,and carbohydrate metabolism-related genes(Sucrose Synthase 2)and simultaneously controlled multiple(at least two or more)seed traits.These results indicate that seed traits are jointly regulated by some genes involved in seed morphology regulation.In this work,we identified new gene loci related to grape seed traits.Identifying molecular markers closely related to these seed traits is of great significance for breeding seedless grape varieties.展开更多
利用Aspen Plus软件对二甲醚(DME)合成装置的工艺流程建立了模拟模型,对该流程进行了物料与热量衡算,并利用Aspen Energy Analyzer软件对DME合成装置的换热网络进行了分析与优化。结果表明:DME合成装置可以得到质量分数为99.95%的DME产...利用Aspen Plus软件对二甲醚(DME)合成装置的工艺流程建立了模拟模型,对该流程进行了物料与热量衡算,并利用Aspen Energy Analyzer软件对DME合成装置的换热网络进行了分析与优化。结果表明:DME合成装置可以得到质量分数为99.95%的DME产品,且该装置的物料与热量均守衡,验证了该工艺的可行性;优化后的DME合成装置工艺流程可节省热物流或冷物流能量10572.3 kW。展开更多
Mineral exploration under exotic overburden has been a great challenge in exploration geochemistry.Gas geochemical measurement is a potential method for mineral exploration due to the characteristics of strong penetra...Mineral exploration under exotic overburden has been a great challenge in exploration geochemistry.Gas geochemical measurement is a potential method for mineral exploration due to the characteristics of strong penetrability and vertical migration.The previous rapid gas analyzer cannot determine low concentrations of soil gases because of inadequate sensitivity,therefore,it is necessary to develop a more sensitive analytical technique.In this paper,the pilot studies on CO_(2) and SO_(2) geochemical measurements were performed at the Zhuxi W-(Cu)deposit,Jiapigou gold ore-concentrated area,and Tukuzbay gold deposit.This study employed rapid gas analyzer based on Infrared Absorption Spectroscopy and Electrochemical Analysis,which can detect low concentrations of CO_(2) and SO_(2) and greatly improve the reliability of test data.The results show that CO_(2) and SO_(2) show clear anomalies over deeply concealed ore bodies and faults,demonstrating that CO_(2) and SO_(2) anomalies can reveal indicative information about concealed mineralization and faults.Moreover,CO_(2) and SO_(2) anomalies can identify mineralization information exceeding 1,000 m in depth,suggesting that this method has large detection depth.Therefore,CO_(2) and SO_(2) geochemical measurement method is a feasible tool to discern deeply concealed mineralization and faults,and can provide a new idea in prospecting for concealed ore deposits in covered areas.展开更多
Extreme-mass-ratio inspiral(EMRI)signals pose significant challenges to gravitational wave(GW)data analysis,mainly owing to their highly complex waveforms and high-dimensional parameter space.Given their extended time...Extreme-mass-ratio inspiral(EMRI)signals pose significant challenges to gravitational wave(GW)data analysis,mainly owing to their highly complex waveforms and high-dimensional parameter space.Given their extended timescales of months to years and low signal-to-noise ratios,detecting and analyzing EMRIs with confidence generally relies on long-term observations.Besides the length of data,parameter estimation is particularly challenging due to non-local parameter degeneracies,arising from multiple local maxima,as well as flat regions and ridges inherent in the likelihood function.These factors lead to exceptionally high time complexity for parameter analysis based on traditional matched filtering and random sampling methods.To address these challenges,the present study explores a machine learning approach to Bayesian posterior estimation of EMRI signals,leveraging the recently developed flow matching technique based on ordinary differential equation neural networks.To our knowledge,this is also the first instance of applying continuous normalizing flows to EMRI analysis.Our approach demonstrates an increase in computational efficiency by several orders of magnitude compared to the traditional Markov chain Monte Carlo(MCMC)methods,while preserving the unbiasedness of results.However,we note that the posterior distributions generated by FMPE may exhibit broader uncertainty ranges than those obtained through full Bayesian sampling,requiring subsequent refinement via methods such as MCMC.Notably,when searching from large priors,our model rapidly approaches the true values while MCMC struggles to converge to the global maximum.Our findings highlight that machine learning has the potential to efficiently handle the vast EMRI parameter space of up to seventeen dimensions,offering new perspectives for advancing space-based GW detection and GW astronomy.展开更多
My creative focus and interest has always been the state of human existence.Compared with grand narratives,it is often the most inadvertent,subtle actions that become my entry point.I starl a dialogue with myself by c...My creative focus and interest has always been the state of human existence.Compared with grand narratives,it is often the most inadvertent,subtle actions that become my entry point.I starl a dialogue with myself by carefully observing and deeply analyzing those subtle yet tense behaviors.Through this process,I discover and develop my own creative system to affirm the value of my existence in my work.展开更多
Sorghum,a drought-resistant cereal crop crucial for global food security,typically produces single seeds per spikelet.A team of researchers from the Institute of Genetics and Developmental Biology(IGDB)of the Chinese ...Sorghum,a drought-resistant cereal crop crucial for global food security,typically produces single seeds per spikelet.A team of researchers from the Institute of Genetics and Developmental Biology(IGDB)of the Chinese Academy of Sciences and the Sun Yat-sen University in Guangzhou recently identified a genetic mechanism that enables some varieties to develop double-grain spikelets,significantly boosting yields.By analyzing three genetic populations,scientists traced this trait to a 35.7-kilobase chromosomal inversion activating DG1-a gene that encodes a protein regulating floral meristem development.展开更多
The rapid development of artificial intelligence(AI),machine learning(ML),and deep learning(DL)in recent years has transformed many sectors.A fundamental shift has occurred in approaches to solving complex problems an...The rapid development of artificial intelligence(AI),machine learning(ML),and deep learning(DL)in recent years has transformed many sectors.A fundamental shift has occurred in approaches to solving complex problems and making decisions in many different fields.These advanced technologies have enabled significant breakthroughs in sectors including entertainment,finance,transportation,and healthcare.AI systems,which can analyze vast volumes of data,have significantly driven efficiency and innovation.With remarkable accuracy,patterns can be identified and predictions generated,improving decision-making processes and facilitating the development of more intelligent solutions.The increasing adoption of these technologies by organizations has expanded the potential for AI to change processes and improve results.展开更多
With the reduction of sequencing costs,optimization of algorithms,and improvement of multi-omics integration capabilities,transcriptomics,as a core technology for analyzing gene expression dynamics and discovering key...With the reduction of sequencing costs,optimization of algorithms,and improvement of multi-omics integration capabilities,transcriptomics,as a core technology for analyzing gene expression dynamics and discovering key functional molecules,has shown great potential in the field of disease prevention and control[1,2].The multi-continental transcriptomics study of tick-borne poxvirus not only provides a new perspective for understanding the evolution and transmission of vector-mediated viruses,but also reflects the trend of transcriptomics research and highlights its key role in disease prevention and control[3].展开更多
基于web of science和知网数据源,利用Derwent Data Analyzer,VOSvievwer和Incites对1960—2022年盐湖相关文献进行计量分析,以深入了解国际和国内盐湖研究进展和发展趋势。分析得出近60多年盐湖研究发文量整体上呈先稳定后增加的趋势;...基于web of science和知网数据源,利用Derwent Data Analyzer,VOSvievwer和Incites对1960—2022年盐湖相关文献进行计量分析,以深入了解国际和国内盐湖研究进展和发展趋势。分析得出近60多年盐湖研究发文量整体上呈先稳定后增加的趋势;盐湖相关研究国际发文数量最高的期刊是International Journal of Systematic and Evolutionary Microbiology,国内发文量最高的期刊是《盐湖研究》。美国和中国是盐湖研究的核心力量,两者的总发文量、总引频次和高被引论文数均居前2位。盐湖研究领域发文量最高的机构是中国科学院,其隶属单位青海盐湖研究所发文量占比最高。国际文献作者前15位中8位来自中国。国际和国内研究热点演进分析得出今后盐湖资源的分离和提取将得到持续关注,高被引文章分析说明近年来盐湖研究关注最多的为盐湖资源的分离提取,尤其是锂资源。整体而言,有关盐湖研究还在不断拓展和延伸,未来我国应在文章质量、研究方向布局和影响力上继续提升,发挥国内盐湖研究的对外影响力。展开更多
基金Baoding Science and Technology Bureau(2141ZF313)。
文摘Objective:To investigate the hook effect in the detection of beta2-microglobulin(β2-MG)with different reagents as well as on two fully automated biochemical analyzers and their solutions.Methods:Using immunoturbidimetric assay forβ2-MG as the research object,β2-MG levels were detected by different concentration gradients ofβ2-microglobulin samples in Liedemann,Mack,and Myriad reagents of three manufacturers on two automatic biochemical analyzers,and the difference of the hook effect was comparatively analyzed.Results:Leadman reagent showed the hook effect on the Beckman AU5800 automated biochemistry analyzer,while both Maccura and Mindray reagents did not show the hook effect.After the experiments,we found the limit value of the pre-zone check of Leadman reagent,and changed the parameters of the instrument,when the limit value of the pre-zone check was reached,the instrument automatically diluted the specimen five times and then detected it again.After changing the parameters of the instrument,the correlation between the three methods of detecting samples of different concentrations was r>0.99.Conclusion:Before selecting the application of immunoturbidimetric reagents,we have to carry out the risk assessment of the hook effect and selectively set the parameters of the pre-zone check based on the highest concentration that may occur in the clinic;for the items that may have the hook effect,we have to selectively set the parameters of the pre-zone check when the ratio of average reaction rate and the immediate reaction rate is at the limit value,and the phenomenon of antigen excess may occur,the instrument will carry out automatic dilution before detection,so as to avoid the issuance of erroneous results of high-value samples due to the hook effect.
文摘Background:Lipemia,characterized by elevated triglyceride levels in blood samples,is a prevalent preanalytical interferent in clinical hematology.It leads to erroneous measurements of key complete blood count(CBC)parameters,including falsely elevated hemoglobin(Hgb)and platelet(PLT)counts.These inaccuracies can compromise diagnostic reliability and patient management.Objective:This review systematically evaluates existing correction methods for lipemic interference in CBC analysis,comparing their efficacy,limitations,and applicability in clinical settings.Methods:We analyze saline replacement,formula-based correction,instrument-specific algorithms,and emerging technologies,supported by experimental and clinical validation data.Conclusion:An optimized,context-dependent strategy is proposed,integrating multiple correction approaches based on lipemia severity.Future research directions,including artificial intelligence(AI)-enhanced corrections and standardized protocols,are discussed to advance hematology testing accuracy.
基金supported by the International Science&Technology Cooperation Program of China(Grant Nos.2015DFG21960&2011DFA21090)the National Natural Science Foundation of China(Grant Nos.40905066,41175116,41273097,41505108&41505123)+2 种基金the CMA Climate Change Program(Grant No.CCSF201331),the CMA Operational Fund(Grant No.CMAGJ2013M73)the Graduate Research and Innovation Projects of Universities in Jiangsu Province(Grant No.KYLX_0834)the CAMS Fundamental Research Funds(Grant Nos.2014Y005,2015Y002&2014Z004)
文摘Non-dispersive infrared （NDIR） and cavity ring-down spectroscopy （CRDS） CO2 analyzers use 12CO2 isotopologue absorption lines and are insensitive to all or part of other CO2-related isotopologues. This may produce biases in CO2 mole fraction measurements of a sample if its carbon isotopic composition deviates from that of the standard gases being used. To evaluate and compare the effects of carbon isotopic composition on NDIR and CRDS CO2 analyzers, we prepared three test sample air cylinders with varying carbon isotopic abundances and calibrated them against five standard cylinders with ambient carbon isotopic composition using CRDS and NDIR systems. We found that the CO2 mole fractions of the sample cylinders measured by G1301 （CRDS） were in good agreement with those measured by LoFlo （NDIR）. The CO2 values measured by both instruments were higher than that of a CO2 isotope measured by G2201i （CRDS） analyzer for a test cylinder with depleted carbon isotopic composition δ^13C =-36.828%0, whereas no obvious difference was found for other two test cylinders with 3 δ^13C=-8.630‰ and δ^13C=-15.380‰, respectively. According to the theoretical and experimental results, we concluded that the total CO2 mole fractions of samples with depleted isotopic compositions can be corrected on the basis of their 12CO2 values calibrated by standard gases using LoFlo and G1301 if the fi13C and fi180 values are known. Keywords NDIR and CRDS analyzers, Carbon isotopic effects, CO2 measurements
文摘Background There is scarce data about comparisons between geriatric assessment tools in patients with aortic stenosis(AS).We aimed to describe the geriatric profile of patients with AS undergoing transcatheter aortic valve implantation(TAVI)and to analyze the ability of different tools for predicting clinical outcomes in this context.Methods This was a single center retrospective registry including patients with AS undergoing TAVI and surviving to hospital discharge.The primary endpoint was all-cause mortality or need for urgent readmission one year after TAVI.Results A total of 377 patients were included(mean age of 80.4 years).Most patients were independent or mildly dependent,with an optimal cognitive status.The proportion of frailty ranged from 17.6%to 49.8%.A total of 20 patients(5.3%)died and 110/377 patients(29.2%)died or were readmitted during follow up.Overall,most components of the geriatric assessment showed an association with clinical outcomes.Disability for instrumental activities showed a significant association with mortality and a strong association with the rate of mortality or readmission.The association between frailty and clinical outcomes was higher for short physical performance battery(SPPB),essential frailty toolset(EFT)and the frailty index based on comprehensive geriatric assessment(IF-VIG)and lower for Fried criteria and FRAIL scale.Conclusions AS patients from this series presented a good physical performance,optimal cognitive status and a reasonably low prevalence of frailty.The best predictive ability was observed for disability for instrumental activities and frailty as measured by the EFT,SPPB and the IF-VIG.
基金Supported by the National Natural Science Foundation of China(42474239,41204128)China National Space Administration(Pre-research project on Civil Aerospace Technologies No.D010301)Strategic Priority Research Program of the Chinese Academy of Sciences(XDA17010303)。
文摘One of the detection objectives of the Chinese Asteroid Exploration mission is to investigate the space environment near the Main-belt Comet(MBC,Active Asteroid)311P/PANSTARRS.This paper outlines the scientific objectives,measurement targets,and measurement requirements for the proposed Gas and Ion Analyzer(GIA).The GIA is designed for in-situ mass spectrometry of neutral gases and low-energy ions,such as hydrogen,carbon,and oxygen,in the vicinity of 311P.Ion sampling techniques are essential for the GIA's Time-of-Flight(TOF)mass analysis capabilities.In this paper,we present an enhanced ion sampling technique through the development of an ion attraction model and an ion source model.The ion attraction model demonstrates that adjusting attraction grid voltage can enhance the detection efficiency of low-energy ions and mitigate the repulsive force of ions during sampling,which is influenced by the satellite's surface positive charging.The ion source model simulates the processes of gas ionization and ion multiplication.Simulation results indicate that the GIA can achieve a lower pressure limit below 10-13Pa and possess a dynamic range exceeding 10~9.These performances ensure the generation of ions with stable and consistent current,which is crucial for high-resolution and broad dynamic range mass spectrometer analysis.Preliminary testing experiments have verified GIA's capability to detect gas compositions such as H2O and N2.In-situ measurements near 311P using GIA are expected to significantly contribute to our understanding of asteroid activity mechanisms,the evolution of the atmospheric and ionized environments of main-belt comets,the interactions with solar wind,and the origin of Earth's water.
文摘A Linear Feedback Shift Register (LFSR)can be used to compress test response data as a Signature Analyzer(SA). Parallel Signature Analyzers (PSAs) implemented as multiple input LFSRs are faster and re- quire less hardware overhead than Serial Signature Analyzers (SSAs)for compacting test response data for Built-In Serf-Test (BIST)in IC or hoard-testing environments. However, the SAs are prone to aliasing errors because of some specific types of error patterns. An alias is a faulty output signature that is identical to the fault-free signature. A penetrating analysis of detecting capability of SAs depends strongly on mathematical manipulations, instead of being aware of some special cases or examples. In addition , the analysis should not be restricted to a particular structure of LFSR, but be appropriate for various structures of LFSRs. This pa- per presents necessary and sufficient conditions for aliasing errors based on a complete mathematical descrip- tion of various types of SAs. An LFSR reconfiguration scheme is suggested which will prevent any aliasing double errors. Such a prevention cannot be obtained by any extension of an LFSR.
文摘The residual biomass composed of pseudo trunks and banana leaves is very important and poorly valued. There is very little quantified data on the deposits of residual biomass from banana plantations in Senegal and in particular in the Tambacounda region. In this work, we seek to evaluate the methanogenic potential and to valorize this biomass in biogas and biofertilizer. The laboratory experiment lasted approximately 35 days. During this time, the methanogenic microorganisms degrade the organic residue provided, which results in the production of biogas. At the end of the reactions, the rate of biogas production drops, indicating the end of the biodegradation of organic matter. Biogas production is measured over time and the composition of the biogas produced is analyzed by gas chromatography (GC) or by an infrared analyzer. The methane potential of each sample is determined from the cumulative quantity of methane produced in each flask representing a digestion system. The measurement can be expressed in m3 of CH4 per tonne of dry matter or per tonne of raw material. The first challenge of this study therefore lies in the acquisition of reliable and usable data to quantify the methanizable biomass. This study will allow us not only to evaluate the quantities of pseudo trunks and banana leaves available in a plot after harvest but also to test the biogas and methane production potential (BMP test) of this substrate and therefore determine the expected biogas production of this biomass.
基金funding by the China Postdoctoral Science Foundation(Grant No.:2021M701628)the National Natural Science Foundation of China(Grant No.:82202625)+3 种基金Science and Technology Projects in Guangzhou,China(Grant No.:SL2022A04J02086)President Foundation of Nanfang Hospital,Southern Medical University,China(Grant Nos.:2021B012,and 2021C050)National Science Fund for Distinguished Young Scholars,China(Grant No.:82025024)Key project of the National Natural Science Foundation of China(Grant No.:82230080).
文摘The level of urinary albumin is a critical indicator for the early diagnosis and management of chronic kidney disease(CKD).However,existing methods for detecting albumin are not conducive to point-of-care testing due to the complexity of reagent addition and incubation processes.This study presents a smartphone-integrated handheld automated biochemical analyzer(sHABA)designed for point-of-care testing of urinary albumin.The sHABA features a pre-loaded,disposable reagent cassette with reagents for the albumin assay arranged in the order of their addition within a hose.The smartphone-integrated analyzer can drive the reagents following a preset program,to enable automatic sequential addition.The sHABA has a detection limit for albumin of 5.9 mg/L and a linear detection range from 7 to 450 mg/L.The consistency of albumin level detection in 931 urine samples using sHABA with clinical tests indicates good sensitivity(95.78%)and specificity(90.16%).This research advances the field by providing an automated detection method for albumin in a portable device,allowing even untrained individuals to monitor CKD in real time at the patient's bedside.In the context of promoting tiered diagnosis and treatment,the sHABA has the potential to become an essential tool for the early diagnosis and comprehensive management of CKD and other chronic conditions.
基金Supported by Noor Ophthalmology Research Center.
文摘Biomechanical study of the visual system by ocular response analyzer investigates the inter-structural biological relationships,mechanics,and function of the visual system.This review aimed to investigate the changes in corneal biomechanical parameters with age and sex.The articles published in PubMed between 2000 and 2021 were investigated and critiqued,and valid scientific evidence was collected,reviewed and concluded according to the inclusion and exclusion criteria.Most studies showed that corneal biomechanical changes occur infrequently in children up to the age of 20y,and with increasing age and wider age range,there was a significant decrease in corneal biomechanical indices,especially corneal hysteresis.In children and adults,most studies have shown that these biomechanical indicators,especially corneal resistance factor,were higher in females.Although hormonal changes may contribute to this finding,the role of axial length and other biometric indicators should not be ignored.The axial length,the intraocular pressure,and the corneal thickness are other factors associated with biomechanical parameters that should be taken into account in clinical diagnosis and management especially for patients undergoing refractive surgery as well as keratoconus patients.
基金The National Key Research and Development Program of China under contract Nos 2022YFC3104203 and 2018YFC0213103the Science Foundation of Donghai Laboratory under contract No.DH-2022KF01019+1 种基金the National Natural Science Foundation under contract No.419061522023 Shanghai Education Science Research Project under contract No.C2023120.
文摘Air-sea water vapor and CO_(2) flux observation experiments were carried out at the Yantai National Satellite Ocean Calibration Platform and the jetty at Monolithic Beach,Juehua Island,using a 100 Hz gas analyzer.The observations were corrected by employing wild point rejection,linear detrending,delay correction,coordinate rotation,time matching,and Webb,Pearman,and Leuning(WPL)correction.The results of spectral analysis and a turbulence development adequacy data quality check showed that the overall observation data quality was good.The air-sea water vapor and CO_(2) flux results showed that the observation duration affected both the air-sea flux intensity and direction at different observation frequencies.At shorter observation durations,the air-sea flux values measured at 100 Hz were smaller than the 20 Hz measurements and had opposite directions.In addition,the WPL correction reduced the overall air-sea flux and partially minimized the effect of observation frequency on the air-sea flux intensity.These results showed that high-frequency observations showed more turbulence variations than low-frequency observations.This conclusion could promote an understanding of small-scale turbulence variations.
基金supported by grants from the Basic Research Business Fees for Public Welfare in Xinjiang Autonomous Region(Mining candidate genes related to grape seed traits based on GWAS,KY2023028)the Xinjiang Uygur Autonomous Region Tianchi Talent-Young Doctor for Chuan Zhang(Revealing the domestication history of Xinjiang native grape varieties and genetic analysis of important agronomic traits)the Xinjiang Academy of Agricultural Sciences Youth Science and Technology Backbone Innovation Ability Training Project(xjnkq-2023006).
文摘Seedlessness has always been a valuable quality characteristic of edible grape varieties.Although the production of seedless grapes has been ongoing for decades,the genetic complexity of seedless grapes is not yet fully understood.Therefore,determining the genetic mechanisms and key regulatory genes of seedless grapes is of great significance for seedless grape breeding and meeting market demands.The emergence of high-throughput analysis software offers greater possibilities for mining genes related to plant organ development.Specifically,to mine a greater number of candidate genes related to grape seed traits,this study used the seed trait parameters analyzed by Tomato Analyzer as the target trait and then used a genome-wide association study(GWAS)to mine candidate genes.In the two-year analysis using principal component analysis(PCA),we extracted five principal components with a cumulative contribution rate of 96.586%.The cumulative contribution rate for component 1 reached 87.352%.Correlation analysis revealed correlation coefficients ranging from 0.54 to 0.98 among the seven basic traits.The GWAS results indicated that 370 SNP loci were significantly correlated with seed traits.These SNP loci were distributed on 18 chromosomes,except for chromosome 4,with most SNP loci distributed on chromosome 18.Based on the physical location of single nucleotide polymorphism(SNP)markers significantly associated with seed-related traits in the grape reference genome,candidate genes are screened within the range of linkage disequilibrium(LD)attenuation distance,both upstream and downstream of the significant SNP loci.These candidate genes were mainly transcription factor-related genes(VvMADS4 and VvMADS5),ubiquitin ligase-related genes(E3 ubiquitin ligase BIG BROTHER),serine/threonine protein kinase-related genes,and carbohydrate metabolism-related genes(Sucrose Synthase 2)and simultaneously controlled multiple(at least two or more)seed traits.These results indicate that seed traits are jointly regulated by some genes involved in seed morphology regulation.In this work,we identified new gene loci related to grape seed traits.Identifying molecular markers closely related to these seed traits is of great significance for breeding seedless grape varieties.
文摘利用Aspen Plus软件对二甲醚(DME)合成装置的工艺流程建立了模拟模型,对该流程进行了物料与热量衡算,并利用Aspen Energy Analyzer软件对DME合成装置的换热网络进行了分析与优化。结果表明:DME合成装置可以得到质量分数为99.95%的DME产品,且该装置的物料与热量均守衡,验证了该工艺的可行性;优化后的DME合成装置工艺流程可节省热物流或冷物流能量10572.3 kW。
基金the Key Laboratory of Geochemical Exploration, Institute of Geophysical and Geochemical Exploration, China Geological Survey, and Ministry of Natural Resources of the People’s Republic of China for funding this researchsupported by National Nonprofit Institute Research Grant of Institute of Geophysical and Geochemical Exploration (AS2022P03)Key Laboratory of Ionic Rare Earth Resources and Environment, Ministry of Natural Resources of the People’s Republic of China (No. 2022IRERE101 and No. 2023IRERE102)
文摘Mineral exploration under exotic overburden has been a great challenge in exploration geochemistry.Gas geochemical measurement is a potential method for mineral exploration due to the characteristics of strong penetrability and vertical migration.The previous rapid gas analyzer cannot determine low concentrations of soil gases because of inadequate sensitivity,therefore,it is necessary to develop a more sensitive analytical technique.In this paper,the pilot studies on CO_(2) and SO_(2) geochemical measurements were performed at the Zhuxi W-(Cu)deposit,Jiapigou gold ore-concentrated area,and Tukuzbay gold deposit.This study employed rapid gas analyzer based on Infrared Absorption Spectroscopy and Electrochemical Analysis,which can detect low concentrations of CO_(2) and SO_(2) and greatly improve the reliability of test data.The results show that CO_(2) and SO_(2) show clear anomalies over deeply concealed ore bodies and faults,demonstrating that CO_(2) and SO_(2) anomalies can reveal indicative information about concealed mineralization and faults.Moreover,CO_(2) and SO_(2) anomalies can identify mineralization information exceeding 1,000 m in depth,suggesting that this method has large detection depth.Therefore,CO_(2) and SO_(2) geochemical measurement method is a feasible tool to discern deeply concealed mineralization and faults,and can provide a new idea in prospecting for concealed ore deposits in covered areas.
基金supported by the National Key Research and Development Program of China(Grant Nos.2021YFC2201901,2021YFC2203004,2020YFC2200100 and 2021YFC2201903)International Partnership Program of the Chinese Academy of Sciences(Grant No.025GJHZ2023106GC)+4 种基金the financial support from Brazilian agencies Funda??o de AmparoàPesquisa do Estado de S?o Paulo(FAPESP)Funda??o de Amparoà Pesquisa do Estado do Rio Grande do Sul(FAPERGS)Fundacao de Amparoà Pesquisa do Estado do Rio de Janeiro(FAPERJ)Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)Coordenacao de Aperfeicoamento de Pessoal de Nível Superior(CAPES)。
文摘Extreme-mass-ratio inspiral(EMRI)signals pose significant challenges to gravitational wave(GW)data analysis,mainly owing to their highly complex waveforms and high-dimensional parameter space.Given their extended timescales of months to years and low signal-to-noise ratios,detecting and analyzing EMRIs with confidence generally relies on long-term observations.Besides the length of data,parameter estimation is particularly challenging due to non-local parameter degeneracies,arising from multiple local maxima,as well as flat regions and ridges inherent in the likelihood function.These factors lead to exceptionally high time complexity for parameter analysis based on traditional matched filtering and random sampling methods.To address these challenges,the present study explores a machine learning approach to Bayesian posterior estimation of EMRI signals,leveraging the recently developed flow matching technique based on ordinary differential equation neural networks.To our knowledge,this is also the first instance of applying continuous normalizing flows to EMRI analysis.Our approach demonstrates an increase in computational efficiency by several orders of magnitude compared to the traditional Markov chain Monte Carlo(MCMC)methods,while preserving the unbiasedness of results.However,we note that the posterior distributions generated by FMPE may exhibit broader uncertainty ranges than those obtained through full Bayesian sampling,requiring subsequent refinement via methods such as MCMC.Notably,when searching from large priors,our model rapidly approaches the true values while MCMC struggles to converge to the global maximum.Our findings highlight that machine learning has the potential to efficiently handle the vast EMRI parameter space of up to seventeen dimensions,offering new perspectives for advancing space-based GW detection and GW astronomy.
文摘My creative focus and interest has always been the state of human existence.Compared with grand narratives,it is often the most inadvertent,subtle actions that become my entry point.I starl a dialogue with myself by carefully observing and deeply analyzing those subtle yet tense behaviors.Through this process,I discover and develop my own creative system to affirm the value of my existence in my work.
文摘Sorghum,a drought-resistant cereal crop crucial for global food security,typically produces single seeds per spikelet.A team of researchers from the Institute of Genetics and Developmental Biology(IGDB)of the Chinese Academy of Sciences and the Sun Yat-sen University in Guangzhou recently identified a genetic mechanism that enables some varieties to develop double-grain spikelets,significantly boosting yields.By analyzing three genetic populations,scientists traced this trait to a 35.7-kilobase chromosomal inversion activating DG1-a gene that encodes a protein regulating floral meristem development.
基金funded by the Research,Development,and Innovation Authority(RDIA),Kingdom of Saudi Arabia,with grant number 13382-PSU-2023-PSNU-R-3-1-EIsupported by the Automated Systems and Computing Lab(ASCL),Prince Sultan University,Riyadh,Saudi Arabia.
文摘The rapid development of artificial intelligence(AI),machine learning(ML),and deep learning(DL)in recent years has transformed many sectors.A fundamental shift has occurred in approaches to solving complex problems and making decisions in many different fields.These advanced technologies have enabled significant breakthroughs in sectors including entertainment,finance,transportation,and healthcare.AI systems,which can analyze vast volumes of data,have significantly driven efficiency and innovation.With remarkable accuracy,patterns can be identified and predictions generated,improving decision-making processes and facilitating the development of more intelligent solutions.The increasing adoption of these technologies by organizations has expanded the potential for AI to change processes and improve results.
文摘With the reduction of sequencing costs,optimization of algorithms,and improvement of multi-omics integration capabilities,transcriptomics,as a core technology for analyzing gene expression dynamics and discovering key functional molecules,has shown great potential in the field of disease prevention and control[1,2].The multi-continental transcriptomics study of tick-borne poxvirus not only provides a new perspective for understanding the evolution and transmission of vector-mediated viruses,but also reflects the trend of transcriptomics research and highlights its key role in disease prevention and control[3].
文摘基于web of science和知网数据源,利用Derwent Data Analyzer,VOSvievwer和Incites对1960—2022年盐湖相关文献进行计量分析,以深入了解国际和国内盐湖研究进展和发展趋势。分析得出近60多年盐湖研究发文量整体上呈先稳定后增加的趋势;盐湖相关研究国际发文数量最高的期刊是International Journal of Systematic and Evolutionary Microbiology,国内发文量最高的期刊是《盐湖研究》。美国和中国是盐湖研究的核心力量,两者的总发文量、总引频次和高被引论文数均居前2位。盐湖研究领域发文量最高的机构是中国科学院,其隶属单位青海盐湖研究所发文量占比最高。国际文献作者前15位中8位来自中国。国际和国内研究热点演进分析得出今后盐湖资源的分离和提取将得到持续关注,高被引文章分析说明近年来盐湖研究关注最多的为盐湖资源的分离提取,尤其是锂资源。整体而言,有关盐湖研究还在不断拓展和延伸,未来我国应在文章质量、研究方向布局和影响力上继续提升,发挥国内盐湖研究的对外影响力。
