A flock of AA breed chickens were reared in peterstme brood-vait chamber using high energy pelleted feed, at 14 days of age, 400 birds were separated into 3 groups randomly as follows:100 birds were exposed to normal ...A flock of AA breed chickens were reared in peterstme brood-vait chamber using high energy pelleted feed, at 14 days of age, 400 birds were separated into 3 groups randomly as follows:100 birds were exposed to normal ambient temperature (20℃) as control group, 150 birds were exposed to low ambient temperature (11℃) in order to induce ascites as treatment I group, another 100 birds were also exposed to low ambient temperature (11℃) and fed the diets containing 1% L-arginine for ascitic prophylactic treatment as treatment I group. The blood samples were collected on 3, 4, 5, 6, 7 wk, respectively, to measure the contents of plasma endothelin (ET-1), angiotensin Ⅱ(Ang Ⅱ) and cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP). The results indicated that the contents of cAMP, cGMP, AngⅡ and the ratio of cAMP/cGMP in treatment I and ascitic broilers were higher than that of correspondent control group(P<0.01, P<0.05), ET-1 of preascitic broilers were higher than that of control group(P<0. 05), while there were insignificant differences with later ascitic broilers, the contents of cAMP and cGMP in treatment Ⅱ were higher than treatment I and control group(P<0.01, P<0.05), whereas, the ratio of cAMP/ cGMP and the contents of Ang Ⅱ were gradually decreased than that of control group (P<0. 05), the contents of ET-1 were not changed. By further analysis, the increased plasma Ang I at low ambient temperature condition in broilers made endothelium cell secretion of increased ET-1, cAMP, cGMP and decreased NO. So low temperature accelarated ascites syndrome in broilers. Supplemented L-arginine can decrease ET-1, cAMP and cAMP/cGMP. It is concluded that cAMP mediated pulmonary hypertension syndrome in broilers.展开更多
Un célèbre virtuose des arts martiaux,une épée mystérieuse,des combats aériens alternants,l'humanité et la justice,ce sont des éléments culturels qui se déploient ...Un célèbre virtuose des arts martiaux,une épée mystérieuse,des combats aériens alternants,l'humanité et la justice,ce sont des éléments culturels qui se déploient dans Tigre et Dragon,film réalisé en 2000 par Ang Lee.En Occident,où le style 《 de cape et d'épée 》 chinois était moins connu,展开更多
Pathological cardiac hypertrophy is an early and significant cardiac structural characteristic that contributes to the onset and progression of heart failure(HF).Its mainly structural feature is the abnormally enlarge...Pathological cardiac hypertrophy is an early and significant cardiac structural characteristic that contributes to the onset and progression of heart failure(HF).Its mainly structural feature is the abnormally enlarged cardiomyocyte.Effective intervention targets for abnormally enlarged cardiomyocyte remain to be identified.Previous studies have shown that the cellular shape and size can be regulated by the actin related protein 2/3(Arp2/3)complex,which is an actin-binding protein complex involved in the actin nucleation and assembly.However,the roles of the Arp2/3 complex in cardiomyocyte hypertrophy remain unknown.Here our study identifies its novel roles in the occurrence and development of cardiomyocyte hypertrophy.We found that mRNA levels of all subunits from the Arp2/3 complex are significantly upregulated(P<0.05)in the angiotensin Ⅱ(Ang Ⅱ)-induced neonatal rat primary and H9c2 cardiomyocyte hypertrophy.Further studies showed that siRNA-directed ARPC 2 silencing inhibits the reactivation of fetal genes and enlargement of cardiomyocyte area induced by Ang Ⅱ in neonatal rat primary cardiomyocytes(NRCMs)and H9c2 cells(P<0.05).In addition,the upstream activators of the Arp2/3 complex including SH3 protein interacting with Nck,90 kD(SPIN90)and Ras-related C3 botulinum toxin substrate 1(Rac1)/WASp family Verprolin-homologous protein-2(WAVE-2)are upregulated(P<0.05)in Ang Ⅱ-induced neonatal rat primary and H9c2 cardiomyocyte hypertrophy,indicating the excessive activation of the Arp2/3 complex.We further show that CK666,a specific Arp2/3 complex inhibitor,prevents the reactivation of fetal genes and the enlargement of cardiomyocyte area induced by Ang Ⅱ in NRCMs and H9c2 cells(P<0.05).Our results reveal that the Arp2/3 complex plays a crucial role in Ang Ⅱ-induced cardiomyocyte hypertrophy,which is beneficial to further studies about the molecular mechanisms by which the Arp2/3 complex regulates pathological cardiac hypertrophy.展开更多
"Kelsang Yanglha,from Xiasima Town,Yadong County,Xigaze City,Xizang."That's how Kelsang Yanglha usually introduce herself.While most people might mention only the province or city where they were born,Ke..."Kelsang Yanglha,from Xiasima Town,Yadong County,Xigaze City,Xizang."That's how Kelsang Yanglha usually introduce herself.While most people might mention only the province or city where they were born,Kelsang would always make the introduction more specific,telling others the full name of her hometown.For her,identity is more than just a provincial or city-level label-it extends down to the small town with three streets where her life story started.展开更多
文摘A flock of AA breed chickens were reared in peterstme brood-vait chamber using high energy pelleted feed, at 14 days of age, 400 birds were separated into 3 groups randomly as follows:100 birds were exposed to normal ambient temperature (20℃) as control group, 150 birds were exposed to low ambient temperature (11℃) in order to induce ascites as treatment I group, another 100 birds were also exposed to low ambient temperature (11℃) and fed the diets containing 1% L-arginine for ascitic prophylactic treatment as treatment I group. The blood samples were collected on 3, 4, 5, 6, 7 wk, respectively, to measure the contents of plasma endothelin (ET-1), angiotensin Ⅱ(Ang Ⅱ) and cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP). The results indicated that the contents of cAMP, cGMP, AngⅡ and the ratio of cAMP/cGMP in treatment I and ascitic broilers were higher than that of correspondent control group(P<0.01, P<0.05), ET-1 of preascitic broilers were higher than that of control group(P<0. 05), while there were insignificant differences with later ascitic broilers, the contents of cAMP and cGMP in treatment Ⅱ were higher than treatment I and control group(P<0.01, P<0.05), whereas, the ratio of cAMP/ cGMP and the contents of Ang Ⅱ were gradually decreased than that of control group (P<0. 05), the contents of ET-1 were not changed. By further analysis, the increased plasma Ang I at low ambient temperature condition in broilers made endothelium cell secretion of increased ET-1, cAMP, cGMP and decreased NO. So low temperature accelarated ascites syndrome in broilers. Supplemented L-arginine can decrease ET-1, cAMP and cAMP/cGMP. It is concluded that cAMP mediated pulmonary hypertension syndrome in broilers.
文摘Un célèbre virtuose des arts martiaux,une épée mystérieuse,des combats aériens alternants,l'humanité et la justice,ce sont des éléments culturels qui se déploient dans Tigre et Dragon,film réalisé en 2000 par Ang Lee.En Occident,où le style 《 de cape et d'épée 》 chinois était moins connu,
文摘Pathological cardiac hypertrophy is an early and significant cardiac structural characteristic that contributes to the onset and progression of heart failure(HF).Its mainly structural feature is the abnormally enlarged cardiomyocyte.Effective intervention targets for abnormally enlarged cardiomyocyte remain to be identified.Previous studies have shown that the cellular shape and size can be regulated by the actin related protein 2/3(Arp2/3)complex,which is an actin-binding protein complex involved in the actin nucleation and assembly.However,the roles of the Arp2/3 complex in cardiomyocyte hypertrophy remain unknown.Here our study identifies its novel roles in the occurrence and development of cardiomyocyte hypertrophy.We found that mRNA levels of all subunits from the Arp2/3 complex are significantly upregulated(P<0.05)in the angiotensin Ⅱ(Ang Ⅱ)-induced neonatal rat primary and H9c2 cardiomyocyte hypertrophy.Further studies showed that siRNA-directed ARPC 2 silencing inhibits the reactivation of fetal genes and enlargement of cardiomyocyte area induced by Ang Ⅱ in neonatal rat primary cardiomyocytes(NRCMs)and H9c2 cells(P<0.05).In addition,the upstream activators of the Arp2/3 complex including SH3 protein interacting with Nck,90 kD(SPIN90)and Ras-related C3 botulinum toxin substrate 1(Rac1)/WASp family Verprolin-homologous protein-2(WAVE-2)are upregulated(P<0.05)in Ang Ⅱ-induced neonatal rat primary and H9c2 cardiomyocyte hypertrophy,indicating the excessive activation of the Arp2/3 complex.We further show that CK666,a specific Arp2/3 complex inhibitor,prevents the reactivation of fetal genes and the enlargement of cardiomyocyte area induced by Ang Ⅱ in NRCMs and H9c2 cells(P<0.05).Our results reveal that the Arp2/3 complex plays a crucial role in Ang Ⅱ-induced cardiomyocyte hypertrophy,which is beneficial to further studies about the molecular mechanisms by which the Arp2/3 complex regulates pathological cardiac hypertrophy.
文摘"Kelsang Yanglha,from Xiasima Town,Yadong County,Xigaze City,Xizang."That's how Kelsang Yanglha usually introduce herself.While most people might mention only the province or city where they were born,Kelsang would always make the introduction more specific,telling others the full name of her hometown.For her,identity is more than just a provincial or city-level label-it extends down to the small town with three streets where her life story started.
