Six aminoglucose conjugates were synthesized by the reaction of aminoglucose with 5-fluorou-racil-1-acetic acid or 5-fluorouracil-1-propanoic acid and confirmed by IR, 1H NMR and elemental analyses. Their antitumor ac...Six aminoglucose conjugates were synthesized by the reaction of aminoglucose with 5-fluorou-racil-1-acetic acid or 5-fluorouracil-1-propanoic acid and confirmed by IR, 1H NMR and elemental analyses. Their antitumor activities against A2780 cells and PC-14 cells were also evaluated.展开更多
Selenium(Se)is one of the essential trace elements for ruminants.The impact of varying dietary Se levels on rumen epithelium and microbiota remains inadequately studied.This study used multi-omics techniques to invest...Selenium(Se)is one of the essential trace elements for ruminants.The impact of varying dietary Se levels on rumen epithelium and microbiota remains inadequately studied.This study used multi-omics techniques to investigate the coordinated response of rumen microbiota and epithelium to dietary glucosamine selenium(GASe)supplementation.Sixty female Wanlin White goats(19.90±2.00 kg)at 4 months of age were randomly divided into 4 groups with 15 replications of 1 goat each.Goats in the control(CON),and the low(L),medium(M)and high does(H)groups were supplemented GASe at 0,0.6,1.2 and 2.4 mg Se/kg,respectively.After a 60-d feeding trial,5 goats per group were randomly selected for slaughter and sampling.The results showed that average daily gain,rumen papilla length,and glutathione peroxidase(GPx)activity in the L group were significantly higher than those in the CON and H groups(quadratic;P<0.05).Microbial analysis revealed that unclassified_Christensenellaceae(quadratic)had higher(P<0.05)abundances in the L group and showed significant positive correlations with rumen papilla length and GPx activity(P<0.05).In contrast,the abundances of pathogenic Campylobacter were highest in the H group(linear or quadratic;P<0.05).Weighted gene co-expression network analysis(WGCNA)identified the gene module MEturquoise and the compound module ME6 as significantly associated with papilla length and GPx activity(P<0.05).Genes in the MEturquoise module were enriched in ribosome,oxidative phosphorylation,RNA polymerase,and nucleotide sugar biosynthesis pathways,while the ME6 module was mainly associated with amino acid and nucleotide metabolism.Multi-omics analysis revealed that metabolic responses bridged transcriptional regulation and microbial dynamics.Additionally,2 microbiota-derived substances,actinorhodin and dimethylpropiothetin,were significantly associated with papilla length and GPx activity(P<0.05).In conclusion,an appropriate amount of GASe can improve goat growth performance,promote rumen epithelial growth and health by enhancing cellular metabolism and increasing beneficial bacteria abundance.However,excessive GASe supplementation may reduce rumen epithelial antioxidative and metabolic capacities,and promote pathogenic bacteria proliferation.Furthermore,this study demonstrates that metabolites mediate the coordinated response of the rumen microbiota and host to dietary GASe supplementation.Supplementation of 0.598 to 0.832 mg Se/kg GASe in the diet is recommended for goats.展开更多
文摘Six aminoglucose conjugates were synthesized by the reaction of aminoglucose with 5-fluorou-racil-1-acetic acid or 5-fluorouracil-1-propanoic acid and confirmed by IR, 1H NMR and elemental analyses. Their antitumor activities against A2780 cells and PC-14 cells were also evaluated.
基金supported by the Anhui Key Research and Development Program 2023z04020003)the Scientific research project of colleges and universities in Anhui Province(2023AH051045)+1 种基金the Anhui Province Meat Goat Breeding Joint Research Project(2021-2025)the China Agriculture Research System(CARS-38).
文摘Selenium(Se)is one of the essential trace elements for ruminants.The impact of varying dietary Se levels on rumen epithelium and microbiota remains inadequately studied.This study used multi-omics techniques to investigate the coordinated response of rumen microbiota and epithelium to dietary glucosamine selenium(GASe)supplementation.Sixty female Wanlin White goats(19.90±2.00 kg)at 4 months of age were randomly divided into 4 groups with 15 replications of 1 goat each.Goats in the control(CON),and the low(L),medium(M)and high does(H)groups were supplemented GASe at 0,0.6,1.2 and 2.4 mg Se/kg,respectively.After a 60-d feeding trial,5 goats per group were randomly selected for slaughter and sampling.The results showed that average daily gain,rumen papilla length,and glutathione peroxidase(GPx)activity in the L group were significantly higher than those in the CON and H groups(quadratic;P<0.05).Microbial analysis revealed that unclassified_Christensenellaceae(quadratic)had higher(P<0.05)abundances in the L group and showed significant positive correlations with rumen papilla length and GPx activity(P<0.05).In contrast,the abundances of pathogenic Campylobacter were highest in the H group(linear or quadratic;P<0.05).Weighted gene co-expression network analysis(WGCNA)identified the gene module MEturquoise and the compound module ME6 as significantly associated with papilla length and GPx activity(P<0.05).Genes in the MEturquoise module were enriched in ribosome,oxidative phosphorylation,RNA polymerase,and nucleotide sugar biosynthesis pathways,while the ME6 module was mainly associated with amino acid and nucleotide metabolism.Multi-omics analysis revealed that metabolic responses bridged transcriptional regulation and microbial dynamics.Additionally,2 microbiota-derived substances,actinorhodin and dimethylpropiothetin,were significantly associated with papilla length and GPx activity(P<0.05).In conclusion,an appropriate amount of GASe can improve goat growth performance,promote rumen epithelial growth and health by enhancing cellular metabolism and increasing beneficial bacteria abundance.However,excessive GASe supplementation may reduce rumen epithelial antioxidative and metabolic capacities,and promote pathogenic bacteria proliferation.Furthermore,this study demonstrates that metabolites mediate the coordinated response of the rumen microbiota and host to dietary GASe supplementation.Supplementation of 0.598 to 0.832 mg Se/kg GASe in the diet is recommended for goats.
