Intracellular protein delivery is vital for the development of therapeutic proteins that act on intracellular targets.Although numerous carriers based on polymers and nanomaterials have been reported to facilitate cel...Intracellular protein delivery is vital for the development of therapeutic proteins that act on intracellular targets.Although numerous carriers based on polymers and nanomaterials have been reported to facilitate cellular internalization of membrane impermeable proteins,it is still a great challenge to intracellularly deliver proteins with different sizes and isoelectric points through small molecule-based protein carrier.Herein,amidinium functionalized pillar[5]arene(AP5)was used as a small molecular carrier to facilitate intracellular delivery of proteins with different sizes and isoelectric points.The densely preorganized amidinium groups on pillar[5]arene skeleton could not only glue proteins together to form AP5@protein complex through multiple salt-bridges,but also promote cellular internalization AP5@protein complex.The bioactivities of the internalized proteins were well-maintained.This study provides a novel,versatile and macrocyclic-molecule based intracellular protein delivery carrier through the preorganization of amidiniums on pillar[5]arene.展开更多
<div style="text-align:justify;"> Series of 1,3-dibenzyl-1H-4,5,6,7-tetrahydro-1,3-diazepinium and 1,4,5,6,7,8-hexahydro-1,3-diazocinium salts derivatives were efficiently synthesized in excellent yiel...<div style="text-align:justify;"> Series of 1,3-dibenzyl-1H-4,5,6,7-tetrahydro-1,3-diazepinium and 1,4,5,6,7,8-hexahydro-1,3-diazocinium salts derivatives were efficiently synthesized in excellent yields by dehydrogenation of the corresponding N,N'<span "="">-dibenzyl aminals employing N-bromosuccinimide (NBS) as dehydrogenating agent under ultrasound irradiation. The present methodology has proven to be simple, efficient and environmentally benign. All novel compounds were identified and characterized by <sup>1</sup>H and <sup>13</sup>C NMR spectra. The synthesized compounds were screened for their antimicrobial activities.展开更多
CONSPECTUS:Clean energy forms the foundation of sustainable development,and among various technologies,photovoltaics-directly converting sunlight into electricity-stand out as one of the most promising and impactful.I...CONSPECTUS:Clean energy forms the foundation of sustainable development,and among various technologies,photovoltaics-directly converting sunlight into electricity-stand out as one of the most promising and impactful.In recent years,it has garnered significant attention and undergone rapid development.Notably,Organic−inorganic Lead Halide Perovskites(OLHPs)have emerged as a breakthrough in this field.After just a decade of research and development,OLHP-based solar cells have achieved power conversion efficiencies(PCEs)exceeding 26%.OLHPs offer a unique combination of solution-based processing,low-cost production,and high efficiency,making them strong competitors to traditional inorganic semiconductor technologies such as silicon-based photovoltaics.展开更多
基金the financial support from the National Natural Science Foundation of China(No.22101310)Fundamental Research Funds for the Central Universities(No.GK202207013)NJU International Fellowship program。
文摘Intracellular protein delivery is vital for the development of therapeutic proteins that act on intracellular targets.Although numerous carriers based on polymers and nanomaterials have been reported to facilitate cellular internalization of membrane impermeable proteins,it is still a great challenge to intracellularly deliver proteins with different sizes and isoelectric points through small molecule-based protein carrier.Herein,amidinium functionalized pillar[5]arene(AP5)was used as a small molecular carrier to facilitate intracellular delivery of proteins with different sizes and isoelectric points.The densely preorganized amidinium groups on pillar[5]arene skeleton could not only glue proteins together to form AP5@protein complex through multiple salt-bridges,but also promote cellular internalization AP5@protein complex.The bioactivities of the internalized proteins were well-maintained.This study provides a novel,versatile and macrocyclic-molecule based intracellular protein delivery carrier through the preorganization of amidiniums on pillar[5]arene.
文摘<div style="text-align:justify;"> Series of 1,3-dibenzyl-1H-4,5,6,7-tetrahydro-1,3-diazepinium and 1,4,5,6,7,8-hexahydro-1,3-diazocinium salts derivatives were efficiently synthesized in excellent yields by dehydrogenation of the corresponding N,N'<span "="">-dibenzyl aminals employing N-bromosuccinimide (NBS) as dehydrogenating agent under ultrasound irradiation. The present methodology has proven to be simple, efficient and environmentally benign. All novel compounds were identified and characterized by <sup>1</sup>H and <sup>13</sup>C NMR spectra. The synthesized compounds were screened for their antimicrobial activities.
基金acknowledge grants(grant numbers LR24F040001,LD22E020002,and LD24E020001)from the Natural Science Foundation of Zhejiang Province of Chinathe National Natural Science Foundation of China(grant number 62274146)+1 种基金the financial support by Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering(grant number 2021SZFR006)support of Key R&D Program of Zhejiang(2024SSYS0061).
文摘CONSPECTUS:Clean energy forms the foundation of sustainable development,and among various technologies,photovoltaics-directly converting sunlight into electricity-stand out as one of the most promising and impactful.In recent years,it has garnered significant attention and undergone rapid development.Notably,Organic−inorganic Lead Halide Perovskites(OLHPs)have emerged as a breakthrough in this field.After just a decade of research and development,OLHP-based solar cells have achieved power conversion efficiencies(PCEs)exceeding 26%.OLHPs offer a unique combination of solution-based processing,low-cost production,and high efficiency,making them strong competitors to traditional inorganic semiconductor technologies such as silicon-based photovoltaics.
