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金花葵AmCHI基因的克隆及生物信息学分析
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作者 楚洪涛 张婷 +3 位作者 李钰莹 王湘仪 刘仲旭 曹丽 《延边大学农学学报》 2025年第3期10-17,共8页
该实验以金花葵叶片为实验材料,通过分子克隆技术成功获得了金花葵类黄酮生物合成途径中的关键基因AmCHI的编码区序列(coding sequences,CDS)及起始密码子ATG前1111bp的启动子区序列,运用生物信息学方法对其序列进行分析。结果表明:在... 该实验以金花葵叶片为实验材料,通过分子克隆技术成功获得了金花葵类黄酮生物合成途径中的关键基因AmCHI的编码区序列(coding sequences,CDS)及起始密码子ATG前1111bp的启动子区序列,运用生物信息学方法对其序列进行分析。结果表明:在金花葵叶片中克隆得到的AmCHI基因包含882bp的CDS,编码293个氨基酸残基,预测其相对分子质量为31.65kDa,等电点为4.78,通过对AmCHI进行系统进化树分析发现AmCHI与木槿HsCHI的同源性最高。在AmCHI启动子区域包含转录起始的基本元件、响应激素和非生物胁迫以及与组织特异性表达相关的顺式作用元件。该研究为阐明AmCHI基因在金花葵黄酮类化合物合成中的功能及调控机制奠定了基础,也为通过分子育种手段提高金花葵黄酮含量提供了理论依据。 展开更多
关键词 金花葵 amchi 基因克隆 生物信息学分析
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Observations on Traditional Phytotherapy among the Inhabitants of Lahaul Valley through Amchi System of Medicine—A Cold Desert Area of Himachal Pradesh in North Western Himalayas, India 被引量:1
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作者 Parveen Kumar Sharma Surender Kumar Thakur +6 位作者 S. Manuja R. K. Rana Pardeep Kumar Sanjay Sharma Jagdish Chand Ashok Singh Krishan Kumar Katoch 《Chinese Medicine》 2011年第3期93-102,共10页
The paper highlights the Phyto-diversity and indigenous uses an ecosystem existing in Lahaul Valley of tribal district of Lahaul and Spiti in Himacjhal Pradesh, India. This is one of the most preferred tourist spot es... The paper highlights the Phyto-diversity and indigenous uses an ecosystem existing in Lahaul Valley of tribal district of Lahaul and Spiti in Himacjhal Pradesh, India. This is one of the most preferred tourist spot especially for foreigners. The cold desert area of India is known for its specific topography, sense climate and unique vegetation. The valley is rich in high value medicinal wealth and local inhabitants (Buddhist) practice the Tebetan Amchi System of Medicine. Keeping in view the continuous pressure on nature, this paper reflects the Phyto-diversity of Valley, with reference to medicinal and aromatic plants and conserva-tion mechanisms to protect the valuable wealth of the valley and also suggests effective ecotourisim planning of the study area. 展开更多
关键词 Diversity MEDICINAL and AROMATIC Plants Ecotourisim Lahaul Valley Indigenous USES Tebetan amchi SYSTEM of MEDICINE
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