Plant invasion poses a serious environmental challenge to freshwater ecosystems. Heavy metal pollution and nutrient enrichment, particularly nitrogen (N) and phosphorus (P), are two additional critical environmental i...Plant invasion poses a serious environmental challenge to freshwater ecosystems. Heavy metal pollution and nutrient enrichment, particularly nitrogen (N) and phosphorus (P), are two additional critical environmental issues known to influence the growth of invasive plants. Notably, the N/P ratio in the environments is often inconstant;however, whether these variations can cause differential responses in invasive plants remains underexplored, despite its crucial importance for understanding plant invasiveness. In this study, we examined the effects of different N/P ratios (4:1, 16:1, 32:1, 64:1) on the growth of a widespread invasive plant Alternanthera philoxeroides in cadmium (Cd) polluted water, while maintaining either a constant N concentration (2 mmol L^(−1)) or a constant P concentration (0.5 mmol L^(−1)). Two control treatments were also included: one without Cd, N, or P, and another with Cd but without N or P. The addition of N and P did not mitigate the inhibitory effects of Cd on A. philoxeroides growth nor did it enhance its tolerance. However, under conditions of constant N concentration, the N/P ratio of 4:1 significantly increased both the main stem length and the number of nodes in A. philoxeroides under Cd stress, potentially enhancing its expansion and clonal growth. Our results suggest that A. philoxeroides exhibits greater sensitivity to variations in the N/P ratio when N concentration is held constant, compared to when P concentration is constant in Cd polluted water. This study investigated the effects of varying N/P ratios on invasive plants under Cd pollution, which has been underexplored in previous research. The findings provide an experimental basis for controlling and preventing the spread of invasive plants in freshwater systems polluted with Cd and experiencing eutrophication.展开更多
[Objective] The aim was to provide theoretical basis for the prevention and control of the invasion of Alternanthera philoxeroides（Mart.）Griseb.[Method] Effects of fragmentation intensity of fresh roots and their bu...[Objective] The aim was to provide theoretical basis for the prevention and control of the invasion of Alternanthera philoxeroides（Mart.）Griseb.[Method] Effects of fragmentation intensity of fresh roots and their burial depth on sprouting and early growth of A.philoxeroides were studied by control test.[Result] More sprouts of A.philoxeroides emerged when the fragmentation intensity of fresh roots was higher,while if the fragmentation intensity of fresh roots was lower,the early growth of A.philoxeroides was more rapid.The soil buried depth had significant effect on fresh root sprouts＇ emergence,but once fresh root sprouts could reach the soil surface and were given enough growth time,even if the fresh roots were buried in different depths,soil buried depth had no significant effect on its young plant growth.[Conclusion] If different fragmentation intensities of fresh roots present,there is a kind of trade-off strategy between root sprouts＇ emergence and plant＇ early growth,by which A.philoxeroides can invade new habitat successfully.To control the invasion of A.philoxeroides,it is critical to prevent its fresh root sprouts from emerging to soil surface,that is,to bury the fresh roots at a further soil depth.展开更多
[Objective] The paper aimed to discuss effects of different concentrations of lead stress on Alternanthera philoxeroides growth and physiological indices,to explore phytoremediation methods for removal of lead polluti...[Objective] The paper aimed to discuss effects of different concentrations of lead stress on Alternanthera philoxeroides growth and physiological indices,to explore phytoremediation methods for removal of lead pollution. [Method] A. philoxeroides in water culture was treated with different concentration of lead,and then,its external injury was observed,its growth and physiological indexes were measured. [Result] A. philoxeroides biomass and chlorophyll content decreased; conductivity and malonaldehyde （MDA ） content were increased; the SOD,POD and CAT activities showed a trend of first increase and then decrease with increase in concentration of lead stress. [Conclusion] With increase in lead concentration,the cell membrane permeability increased and MDA had a higher accumulation,antioxidant enzymes activity decreased,the normal oxidative metabolism of A. philoxeroides was severely affected,leading it to wilt.展开更多
A water-soluble substance was extracted from the Chinese herb, Alternantheraphiloxcroides with hot water and alcohol. Aliquots of this initial extract were further fractionated by treatment with ether, ethyl acetate a...A water-soluble substance was extracted from the Chinese herb, Alternantheraphiloxcroides with hot water and alcohol. Aliquots of this initial extract were further fractionated by treatment with ether, ethyl acetate and alcohol respectively. The four extracts were assayed for anti-viral activity against three serum, Hantaan virus 114 （HV114）, HV435 and A9 strains. Results show that the four extracts are capable of inhibiting Hantaan virus propagation, of which extract No. 1 has the best efficiency. The three dosage of extract No. 1, which are used upon three Hantaan virus serum IC50, are 153, 157, 154 μg/mL. New-born mice were made to be infected with HV114 and then fed in vivo with extract No.l on the 3rd, 10th and 14th days after being infected by the virus. The treatment continued for 8 days with a dosage of 2.5 g/kg. Result shows that survival rates of mice were 75%, 50% and 0, respectively. The median time to death （MTDs） of the three groups were 37, 30, 23 days.展开更多
Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts(aerial, l...Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts(aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extracts from the plant parts were prepared by maceration technique using 80% ethanol. The anticancer activities were determined using MTT, clonogenic, cell motility and AOPI assay. The chemical composition profiling was analyzed by GC-MS. Results: Among three plant part extracts, leaf extract greatly suppressed the growth of colon cancer cells in time and dosage-dependent manner, followed by aerial and stem. The cytotoxicity results were rationalized with clonogenic, cell motility and AO/PI assay, where extract showed the most active activity compared to aerial and stem extracts. GC-MS analysis of leaf extract showed there were various recognized anti-cancer, anti-oxidant and anti-inflammatory compounds. Conclusions: Amid the screened extracts, the leaf extract exhibits the credible cytotoxic, anti-proliferative and apoptotic activity and hence, our findings call for additional research to conclude the active compounds and their mechanisms determining the apoptotic activity.展开更多
The occurrence and distribution of Alternanthera philoxeroides in different habitats of four districts and counties in the Youjiang River Valley were investigated from September to November in 2018. The results show t...The occurrence and distribution of Alternanthera philoxeroides in different habitats of four districts and counties in the Youjiang River Valley were investigated from September to November in 2018. The results show that A. philoxeroides certainly has occurred in watercourses, ridges, ditches, roadsides and green belts in those four districts and counties of The Youjiang valley. According to the sampling investigation, ridges have the highest frequency of occurrence, followed by ditches and roadsides. The A. philoxeroides living in ditches grows better than other four habitats, and the plants are stronger and vigorous. In the geographical division, the distribution areas of A. philoxeroides in Tianyang District and Tiandong County are the largest. Youjiang District and Pingguo County have a small amount of A. philoxeroides distributed along farmland, green belts and village houses, which has the characteristics of high local population density and strong aggregation, but the areas are smaller than other counties.展开更多
Aims Invasive species occurrence and their effects on biodiversity may vary along latitudes.We examined the occurrence(species cover)and relative dominance(importance value)of invasive alligator weed,Alternanthera phi...Aims Invasive species occurrence and their effects on biodiversity may vary along latitudes.We examined the occurrence(species cover)and relative dominance(importance value)of invasive alligator weed,Alternanthera philoxeroides,in its terrestrial habitat in China through a large-scale latitudinal field investigation.Methods We established 59 plots along the latitudinal transect from 21°N to 37°N.We recorded species name,abundance,height and individual species coverage of plants in every quadrat.We then measuredα-species diversity variations associated with the A.philoxeroides community across the latitudinal range.We also analyzed the effect of latitude on plant species’distributions in this community by using canonical correspondence analysis(CCA).Important Findings We found that species cover and importance value of A.philoxe-roides increased in areas<35°N,but decreased at higher latitudes.Lower latitudes supported greater species diversity than higher latitudes.Small-scale invasion of A.philoxeroides was associated with higher species diversity,but community diversity was lower when A.philoxeroides species cover exceeded 36%.Community plant species changed from mesophyte to hygrophyte gradually from low to high latitude.Our research suggests that latitude had significant influences on community diversity which interacted with the biotic resistance of a community and impact of invasion.Consequently,A.philoxeroides may become more invasive and have greater negative impacts on community species diversity in higher latitudes as global climate changes.展开更多
This study evaluated the physiological characteristics(e.g.,growth parameters,chlorophyll content,metabolites and antioxidative enzymes activity)of Alternanthera philoxeroides(A.philoxeroides),as a hyperaccumulator pl...This study evaluated the physiological characteristics(e.g.,growth parameters,chlorophyll content,metabolites and antioxidative enzymes activity)of Alternanthera philoxeroides(A.philoxeroides),as a hyperaccumulator plant,during the phytoremediation of cadmium(Cd)from water.After cultivating A.philoxeroides in a Cd-containing medium for 30 days,the growth rate was inhibited by up to 33.5%as the exposed Cd concentration increased to 0.80 mmol·L−1.Cd exposure interfered with the photosynthesis of A.philoxeroides and caused oxidative stress as indicated by the rise of malondialdehyde(MDA)and H2O2,which increased by 8 times and 3 times compared to the control group.Moreover,high exposure concentrations of Cd also reduced the activities of multiple antioxidants(e.g.,GSH and AsA),indicating the inhibition of Cd on the plant’s ability to mitigate oxidative damage.Finally,the fluorescent patterns of the rhizosphere dissolved organic matter(rDOM)revealed three major components(humic,fulvic substances and protein-like substances)well correlated with the changes in antioxidant activities.Partial least-squares discriminant analysis(PLS-DA)visualized the difference in the activity of the antioxidative enzymes between different groups.The study unravelled deep insights into the potential mechanisms of tolerance and resistance of A.philoxeroides for phytoremediation of Cd pollution.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32101264)the Science and Technology Research and Development Project of Handan(Grant 23422304086)the National College Student Innovation Training Project of China(Grant No.202410103013).
文摘Plant invasion poses a serious environmental challenge to freshwater ecosystems. Heavy metal pollution and nutrient enrichment, particularly nitrogen (N) and phosphorus (P), are two additional critical environmental issues known to influence the growth of invasive plants. Notably, the N/P ratio in the environments is often inconstant;however, whether these variations can cause differential responses in invasive plants remains underexplored, despite its crucial importance for understanding plant invasiveness. In this study, we examined the effects of different N/P ratios (4:1, 16:1, 32:1, 64:1) on the growth of a widespread invasive plant Alternanthera philoxeroides in cadmium (Cd) polluted water, while maintaining either a constant N concentration (2 mmol L^(−1)) or a constant P concentration (0.5 mmol L^(−1)). Two control treatments were also included: one without Cd, N, or P, and another with Cd but without N or P. The addition of N and P did not mitigate the inhibitory effects of Cd on A. philoxeroides growth nor did it enhance its tolerance. However, under conditions of constant N concentration, the N/P ratio of 4:1 significantly increased both the main stem length and the number of nodes in A. philoxeroides under Cd stress, potentially enhancing its expansion and clonal growth. Our results suggest that A. philoxeroides exhibits greater sensitivity to variations in the N/P ratio when N concentration is held constant, compared to when P concentration is constant in Cd polluted water. This study investigated the effects of varying N/P ratios on invasive plants under Cd pollution, which has been underexplored in previous research. The findings provide an experimental basis for controlling and preventing the spread of invasive plants in freshwater systems polluted with Cd and experiencing eutrophication.
基金Supported by Program from Hubei Education Department(Z200512002)Outstanding Youth Science and Technology Innovation Team Plan Project of Yangtze University~~
文摘[Objective] The aim was to provide theoretical basis for the prevention and control of the invasion of Alternanthera philoxeroides（Mart.）Griseb.[Method] Effects of fragmentation intensity of fresh roots and their burial depth on sprouting and early growth of A.philoxeroides were studied by control test.[Result] More sprouts of A.philoxeroides emerged when the fragmentation intensity of fresh roots was higher,while if the fragmentation intensity of fresh roots was lower,the early growth of A.philoxeroides was more rapid.The soil buried depth had significant effect on fresh root sprouts＇ emergence,but once fresh root sprouts could reach the soil surface and were given enough growth time,even if the fresh roots were buried in different depths,soil buried depth had no significant effect on its young plant growth.[Conclusion] If different fragmentation intensities of fresh roots present,there is a kind of trade-off strategy between root sprouts＇ emergence and plant＇ early growth,by which A.philoxeroides can invade new habitat successfully.To control the invasion of A.philoxeroides,it is critical to prevent its fresh root sprouts from emerging to soil surface,that is,to bury the fresh roots at a further soil depth.
基金Supported by National Natural Science Foundation of China(31060031)Youth Foundation of Yunnan University of Nationalities(09QN02)~~
文摘[Objective] The paper aimed to discuss effects of different concentrations of lead stress on Alternanthera philoxeroides growth and physiological indices,to explore phytoremediation methods for removal of lead pollution. [Method] A. philoxeroides in water culture was treated with different concentration of lead,and then,its external injury was observed,its growth and physiological indexes were measured. [Result] A. philoxeroides biomass and chlorophyll content decreased; conductivity and malonaldehyde （MDA ） content were increased; the SOD,POD and CAT activities showed a trend of first increase and then decrease with increase in concentration of lead stress. [Conclusion] With increase in lead concentration,the cell membrane permeability increased and MDA had a higher accumulation,antioxidant enzymes activity decreased,the normal oxidative metabolism of A. philoxeroides was severely affected,leading it to wilt.
基金Supported by the Natural Science Foundation of Hubei Province (2001ABB162)
文摘A water-soluble substance was extracted from the Chinese herb, Alternantheraphiloxcroides with hot water and alcohol. Aliquots of this initial extract were further fractionated by treatment with ether, ethyl acetate and alcohol respectively. The four extracts were assayed for anti-viral activity against three serum, Hantaan virus 114 （HV114）, HV435 and A9 strains. Results show that the four extracts are capable of inhibiting Hantaan virus propagation, of which extract No. 1 has the best efficiency. The three dosage of extract No. 1, which are used upon three Hantaan virus serum IC50, are 153, 157, 154 μg/mL. New-born mice were made to be infected with HV114 and then fed in vivo with extract No.l on the 3rd, 10th and 14th days after being infected by the virus. The treatment continued for 8 days with a dosage of 2.5 g/kg. Result shows that survival rates of mice were 75%, 50% and 0, respectively. The median time to death （MTDs） of the three groups were 37, 30, 23 days.
文摘Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts(aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extracts from the plant parts were prepared by maceration technique using 80% ethanol. The anticancer activities were determined using MTT, clonogenic, cell motility and AOPI assay. The chemical composition profiling was analyzed by GC-MS. Results: Among three plant part extracts, leaf extract greatly suppressed the growth of colon cancer cells in time and dosage-dependent manner, followed by aerial and stem. The cytotoxicity results were rationalized with clonogenic, cell motility and AO/PI assay, where extract showed the most active activity compared to aerial and stem extracts. GC-MS analysis of leaf extract showed there were various recognized anti-cancer, anti-oxidant and anti-inflammatory compounds. Conclusions: Amid the screened extracts, the leaf extract exhibits the credible cytotoxic, anti-proliferative and apoptotic activity and hence, our findings call for additional research to conclude the active compounds and their mechanisms determining the apoptotic activity.
基金Supported by National Natural Science Foundation of China(31660171)The Basic Ability Improving Project of Young and Middle-aged Teachers in Guangxi(KY2016YB421)2013 Backbone Teacher Project of Baise University(210203009)
文摘The occurrence and distribution of Alternanthera philoxeroides in different habitats of four districts and counties in the Youjiang River Valley were investigated from September to November in 2018. The results show that A. philoxeroides certainly has occurred in watercourses, ridges, ditches, roadsides and green belts in those four districts and counties of The Youjiang valley. According to the sampling investigation, ridges have the highest frequency of occurrence, followed by ditches and roadsides. The A. philoxeroides living in ditches grows better than other four habitats, and the plants are stronger and vigorous. In the geographical division, the distribution areas of A. philoxeroides in Tianyang District and Tiandong County are the largest. Youjiang District and Pingguo County have a small amount of A. philoxeroides distributed along farmland, green belts and village houses, which has the characteristics of high local population density and strong aggregation, but the areas are smaller than other counties.
基金Knowledge Innovation Program of Wuhan Botanical Garden(Y455437H05)supported by a National Science Foundation Postdoctoral Research Fellowship.
文摘Aims Invasive species occurrence and their effects on biodiversity may vary along latitudes.We examined the occurrence(species cover)and relative dominance(importance value)of invasive alligator weed,Alternanthera philoxeroides,in its terrestrial habitat in China through a large-scale latitudinal field investigation.Methods We established 59 plots along the latitudinal transect from 21°N to 37°N.We recorded species name,abundance,height and individual species coverage of plants in every quadrat.We then measuredα-species diversity variations associated with the A.philoxeroides community across the latitudinal range.We also analyzed the effect of latitude on plant species’distributions in this community by using canonical correspondence analysis(CCA).Important Findings We found that species cover and importance value of A.philoxe-roides increased in areas<35°N,but decreased at higher latitudes.Lower latitudes supported greater species diversity than higher latitudes.Small-scale invasion of A.philoxeroides was associated with higher species diversity,but community diversity was lower when A.philoxeroides species cover exceeded 36%.Community plant species changed from mesophyte to hygrophyte gradually from low to high latitude.Our research suggests that latitude had significant influences on community diversity which interacted with the biotic resistance of a community and impact of invasion.Consequently,A.philoxeroides may become more invasive and have greater negative impacts on community species diversity in higher latitudes as global climate changes.
基金supported by National Natural Science Foundation of China(52070122,42007176)Taishan Scholar Foundation,Natural Science Foundation of Shandong Province(ZR2020QD125,ZR2020ME222)+1 种基金Key Scientific and Technological Innovation Foundation of Shandong Province(No.2020CXGC011404)the program of 20 Policies for University in Jinan(202228056).
文摘This study evaluated the physiological characteristics(e.g.,growth parameters,chlorophyll content,metabolites and antioxidative enzymes activity)of Alternanthera philoxeroides(A.philoxeroides),as a hyperaccumulator plant,during the phytoremediation of cadmium(Cd)from water.After cultivating A.philoxeroides in a Cd-containing medium for 30 days,the growth rate was inhibited by up to 33.5%as the exposed Cd concentration increased to 0.80 mmol·L−1.Cd exposure interfered with the photosynthesis of A.philoxeroides and caused oxidative stress as indicated by the rise of malondialdehyde(MDA)and H2O2,which increased by 8 times and 3 times compared to the control group.Moreover,high exposure concentrations of Cd also reduced the activities of multiple antioxidants(e.g.,GSH and AsA),indicating the inhibition of Cd on the plant’s ability to mitigate oxidative damage.Finally,the fluorescent patterns of the rhizosphere dissolved organic matter(rDOM)revealed three major components(humic,fulvic substances and protein-like substances)well correlated with the changes in antioxidant activities.Partial least-squares discriminant analysis(PLS-DA)visualized the difference in the activity of the antioxidative enzymes between different groups.The study unravelled deep insights into the potential mechanisms of tolerance and resistance of A.philoxeroides for phytoremediation of Cd pollution.
