BACKGROUND Massive rotator cuff tears(RCTs)result in impaired shoulder function and quality of life.These tears lead to structural changes in the rotator cuff muscles,which compromise recovery after repair and increas...BACKGROUND Massive rotator cuff tears(RCTs)result in impaired shoulder function and quality of life.These tears lead to structural changes in the rotator cuff muscles,which compromise recovery after repair and increase re-tear rates.AIM To investigate the potential inhibitory effects of alpha-tocopherol(vitamin E)and OTR-4131 on muscle atrophy,fatty infiltration,and fibrosis in rotator cuff muscles following a massive RCT using a Wistar rat model,and establish a standardized methodology for evaluating potential therapeutic agents.METHODS This protocol outlines a controlled animal study using 40 male Wistar rats,randomized into five groups.The experimental groups will receive either systemic administration of alpha-tocopherol or local administration of OTR-4131 via intramuscular injection into the supraspinatus and infraspinatus muscles.Two sham groups will receive systemic and local saline injections respectively,while a control group will undergo no intervention.The interventions will be administered after surgical transection of the supraspinatus and infraspinatus tendons.Outcomes will be assessed via wet muscle weight measurements,muscle fiber diameter,fatty infiltration percentage,and fibrosis evaluation using histological methods.RESULTS The study anticipates that alpha-tocopherol and OTR-4131 will reduce muscle atrophy,fatty infiltration,and fibrosis compared to control and sham groups,supporting their potential protective role in rotator cuff muscle degeneration.CONCLUSION The results are expected to improve the understanding on the role of alpha-tocopherol and OTR-4131 in rotator cuff muscle protection after massive RCT and may serve as a foundation for further preclinical and clinical research aimed at improving rotator cuff repair outcomes.展开更多
<strong>Background</strong>: Many literature reviews report vitamin E supplementation as a benefit chemopreventive and adjuvant therapy in breast and prostate cancers. We investigated in the present work, ...<strong>Background</strong>: Many literature reviews report vitamin E supplementation as a benefit chemopreventive and adjuvant therapy in breast and prostate cancers. We investigated in the present work, alpha-tocopherol (<span style="white-space:nowrap;">α</span>-T) expression (the current active form of vitamin E) from tissues of Congolese patients neither smokers nor alcohol drinkers and without intake antioxidant vitamins supplement. <strong>Methods</strong>: <span style="white-space:nowrap;">α</span>-T was analysed in one normal placenta of nine weeks of gestation and in nineteen cancerous tissues, including seven breast cancers, six prostate cancers, and six gestational choriocarcinomas. The study was performed by immunohistochemistry method after diagnosis confirmation by histological analysis. <strong>Results</strong>: <span style="white-space:nowrap;">α</span>-T staining in membrane cells and collagen fibers presented a moderate expression in healthy sections of tissues (positive control), but the labelling was strong in breast, prostate adenocarcinomas, and in choriocarcinomas. <strong>Conclusion</strong>: Tumors immunohistochemistry of <span style="white-space:nowrap;">α</span>-tocopherol in breast, prostate cancers and in choriocarcinoma show elevated immunostaining suggesting a probable oncogenic effect of the micronutrient.展开更多
Objective:To observe the effects of vitamin E on post-vitrification preimplantation development,gross morphology as well as mitochondrial distribution and ultrastructure.Methods:Twenty-four female C57BL/6NTac mice,age...Objective:To observe the effects of vitamin E on post-vitrification preimplantation development,gross morphology as well as mitochondrial distribution and ultrastructure.Methods:Twenty-four female C57BL/6NTac mice,aged 12-16 weeks,were randomly divided into four groups.Group A did not receive any treatment and served as the control group.Group B was treated with corn oil stripped of tocopherols and served as the vehicle group.Group C was treated with 60 mg/kg body weight of tocotrienol-rich-fraction with corn oil stripped of tocopherols.Group D was treated with 60 mg/kg body weight of alpha-tocopherol with corn oil stripped of tocopherols.All treatments were administered orally for 7 consecutive days.After superovulation and mating with fertile males,2-cell stage embryos were harvested for vitrification.Post vitrification development in vitro,gross morphology and ultrastructure were compared between groups.Results:The number of 2 and 8-cell embryo,and blastocysts in the treatment and control groups were not significantly different(P>0.05).Following vitrification,all 2-cell embryos had equal-sized blastomeres and intact zona pellucida.Mitochondrial aggregation toward the perinuclear region was seen in all of the treatment groups.Both groups C and D had vacuolated mitochondria,which was reflected in the trend of preimplantation development reduction.Conclusions:Vitamin E supplementation of 60 mg/kg body weight does not improve the viability of healthy embryos according to this study.As a result,the most effective dose of vitamin E supplementation may be determined by the initial quality of the embryos.展开更多
The supplementation of vitamin E on broiler chicken diets is essential to the prevention of lipid oxidation reactions in the meat and improvement of meat quality.The objective of this study was to assess the effect of...The supplementation of vitamin E on broiler chicken diets is essential to the prevention of lipid oxidation reactions in the meat and improvement of meat quality.The objective of this study was to assess the effect of different doses of dietary vitamin E on breast meat quality of broiler chickens in the finishing period.Five doses of vitamin E were used(30,90,150,210,and 270 mg/kg feed)in broilers'diets from 42 to 54 d of age.A completely randomized design was conducted,followed by a split-plot,where the vitamin E dose was considered as the whole plot,and broilers'age at slaughter was the subplot.Breast meat quality was assessed at 4 different ages(45,48,51,and 54 d old),using 50 birds per age,totaling 200 birds.Meat quality characteristics evaluated were:pH at 24 h post mortem,color(brightness,redness,and yellowness),water holding capacity,cooking loss,shear force,and lipid peroxidation.There was no interaction between age and dose of vitamin E for meat quality characteristics(P>0.05).The age at slaughter had a quadratic effect(P<0.05)on pH,brightness,redness,and water holding capacity.Although pH values were higher in the breast meat of older birds(51 and 52 d old),breast meat of younger birds(48 d)had a more reddish aspect.Shear force value was higher in breast meat of birds slaughtered at later ages(P<0.01),as a linear age-effect was observed.Brightness increased linearly(P<0.05)with higher vitamin doses,whereas treatments did not alter yellowness,cooking loss,and lipid peroxidation.In this study,increasing vitamin E doses in the finishing period increased the brightness of broiler breast meat,whereas slaughtering at later ages resulted in greater meat pH and shear force value.展开更多
基金thank the staff of the accredited animal facility of the laboratory of anatomy,Histology and Embryology of Aristotle University of Thessaloniki’s veterinary school for their assistance in animal handling and care.
文摘BACKGROUND Massive rotator cuff tears(RCTs)result in impaired shoulder function and quality of life.These tears lead to structural changes in the rotator cuff muscles,which compromise recovery after repair and increase re-tear rates.AIM To investigate the potential inhibitory effects of alpha-tocopherol(vitamin E)and OTR-4131 on muscle atrophy,fatty infiltration,and fibrosis in rotator cuff muscles following a massive RCT using a Wistar rat model,and establish a standardized methodology for evaluating potential therapeutic agents.METHODS This protocol outlines a controlled animal study using 40 male Wistar rats,randomized into five groups.The experimental groups will receive either systemic administration of alpha-tocopherol or local administration of OTR-4131 via intramuscular injection into the supraspinatus and infraspinatus muscles.Two sham groups will receive systemic and local saline injections respectively,while a control group will undergo no intervention.The interventions will be administered after surgical transection of the supraspinatus and infraspinatus tendons.Outcomes will be assessed via wet muscle weight measurements,muscle fiber diameter,fatty infiltration percentage,and fibrosis evaluation using histological methods.RESULTS The study anticipates that alpha-tocopherol and OTR-4131 will reduce muscle atrophy,fatty infiltration,and fibrosis compared to control and sham groups,supporting their potential protective role in rotator cuff muscle degeneration.CONCLUSION The results are expected to improve the understanding on the role of alpha-tocopherol and OTR-4131 in rotator cuff muscle protection after massive RCT and may serve as a foundation for further preclinical and clinical research aimed at improving rotator cuff repair outcomes.
文摘<strong>Background</strong>: Many literature reviews report vitamin E supplementation as a benefit chemopreventive and adjuvant therapy in breast and prostate cancers. We investigated in the present work, alpha-tocopherol (<span style="white-space:nowrap;">α</span>-T) expression (the current active form of vitamin E) from tissues of Congolese patients neither smokers nor alcohol drinkers and without intake antioxidant vitamins supplement. <strong>Methods</strong>: <span style="white-space:nowrap;">α</span>-T was analysed in one normal placenta of nine weeks of gestation and in nineteen cancerous tissues, including seven breast cancers, six prostate cancers, and six gestational choriocarcinomas. The study was performed by immunohistochemistry method after diagnosis confirmation by histological analysis. <strong>Results</strong>: <span style="white-space:nowrap;">α</span>-T staining in membrane cells and collagen fibers presented a moderate expression in healthy sections of tissues (positive control), but the labelling was strong in breast, prostate adenocarcinomas, and in choriocarcinomas. <strong>Conclusion</strong>: Tumors immunohistochemistry of <span style="white-space:nowrap;">α</span>-tocopherol in breast, prostate cancers and in choriocarcinoma show elevated immunostaining suggesting a probable oncogenic effect of the micronutrient.
基金This research was supported financially by Universiti Teknologi MARA Grants[600-IRMI/REI/5/3(022/2019)]and[600-IRMI/MYRA 5/3/MITRA(008/2017)-2].
文摘Objective:To observe the effects of vitamin E on post-vitrification preimplantation development,gross morphology as well as mitochondrial distribution and ultrastructure.Methods:Twenty-four female C57BL/6NTac mice,aged 12-16 weeks,were randomly divided into four groups.Group A did not receive any treatment and served as the control group.Group B was treated with corn oil stripped of tocopherols and served as the vehicle group.Group C was treated with 60 mg/kg body weight of tocotrienol-rich-fraction with corn oil stripped of tocopherols.Group D was treated with 60 mg/kg body weight of alpha-tocopherol with corn oil stripped of tocopherols.All treatments were administered orally for 7 consecutive days.After superovulation and mating with fertile males,2-cell stage embryos were harvested for vitrification.Post vitrification development in vitro,gross morphology and ultrastructure were compared between groups.Results:The number of 2 and 8-cell embryo,and blastocysts in the treatment and control groups were not significantly different(P>0.05).Following vitrification,all 2-cell embryos had equal-sized blastomeres and intact zona pellucida.Mitochondrial aggregation toward the perinuclear region was seen in all of the treatment groups.Both groups C and D had vacuolated mitochondria,which was reflected in the trend of preimplantation development reduction.Conclusions:Vitamin E supplementation of 60 mg/kg body weight does not improve the viability of healthy embryos according to this study.As a result,the most effective dose of vitamin E supplementation may be determined by the initial quality of the embryos.
文摘The supplementation of vitamin E on broiler chicken diets is essential to the prevention of lipid oxidation reactions in the meat and improvement of meat quality.The objective of this study was to assess the effect of different doses of dietary vitamin E on breast meat quality of broiler chickens in the finishing period.Five doses of vitamin E were used(30,90,150,210,and 270 mg/kg feed)in broilers'diets from 42 to 54 d of age.A completely randomized design was conducted,followed by a split-plot,where the vitamin E dose was considered as the whole plot,and broilers'age at slaughter was the subplot.Breast meat quality was assessed at 4 different ages(45,48,51,and 54 d old),using 50 birds per age,totaling 200 birds.Meat quality characteristics evaluated were:pH at 24 h post mortem,color(brightness,redness,and yellowness),water holding capacity,cooking loss,shear force,and lipid peroxidation.There was no interaction between age and dose of vitamin E for meat quality characteristics(P>0.05).The age at slaughter had a quadratic effect(P<0.05)on pH,brightness,redness,and water holding capacity.Although pH values were higher in the breast meat of older birds(51 and 52 d old),breast meat of younger birds(48 d)had a more reddish aspect.Shear force value was higher in breast meat of birds slaughtered at later ages(P<0.01),as a linear age-effect was observed.Brightness increased linearly(P<0.05)with higher vitamin doses,whereas treatments did not alter yellowness,cooking loss,and lipid peroxidation.In this study,increasing vitamin E doses in the finishing period increased the brightness of broiler breast meat,whereas slaughtering at later ages resulted in greater meat pH and shear force value.
