A new flavonol glycoside, isorhamnetin-3-O-b-D-xyloside, was isolated from the extract of leaves and twigs of Alchornea davidii (Euphorbiaceae). Its structure was established on the basis of the spectral analysis and ...A new flavonol glycoside, isorhamnetin-3-O-b-D-xyloside, was isolated from the extract of leaves and twigs of Alchornea davidii (Euphorbiaceae). Its structure was established on the basis of the spectral analysis and chemical degradation. Antimicrobial assay showed that it moderately inhibited the growth of test bacteria (Staphylococcus aureus, Bacillus subtilis and Pseudomonas fluorescens) and fungi (Candida albicans, Aspergillus niger and Trichophyton rubrum) with MICs at 50 g/mL.展开更多
Objective:To investigate the hepatoprotective and antioxidant activities of Alchornea cordifolia (A.cordifolia) leaf extract.Methods:Various solvent fractions of the methanol extract of the leaf of the plant.4.cordifo...Objective:To investigate the hepatoprotective and antioxidant activities of Alchornea cordifolia (A.cordifolia) leaf extract.Methods:Various solvent fractions of the methanol extract of the leaf of the plant.4.cordifolia Mull.Arg(Fam:Euphorbiaceae) were evaluated for hepatoprotective activity by carbon tetrachloride-induced liver damage in rats.The degree of protection was measured by using biochemical parameters such as serum glutamate oxalate transaminase(SGOT/ AST),serum glutamate pyruvate transaminase(SCPT/ALT),alkaline phosphatase(ALP) and total bilirubin.The in vitro antioxidant activity of the extract was also evaluated by the I,I-diphenyl 2-picrylhydrazyl(DPPH) free radical scavenging assay.The extract was subjected to preliminary phytoehemical screening.Results:The ethyl acetate and chloroform fractions,at a dose of 300 mg/kg,produced significant(P【0.05) hepatoprolection by decreasing the activities of the serum enzymes and bilirubin while there were marked scavenging of the DPPH free radicals by the fractions.The effects were comparable to those of the standard drugs used for the respective experiments,silymarin and ascorbic acid.Alkaloids,flavonoids,saponins and tannins were delected in the phylochemical screening.Conclusion:From this study,it was concluded that the plant of A.cordifolia possesses hepatoprotective as well as antioxidant activities and these activities reside mainly in the ethyl acetate and acetone fractions of methanol leaf extract.展开更多
Objective:Alchornea floribunda leaves are widely used in ethnomedicinal management of inflammatory disorders. The present work is aimed at investigating this folkloric use.Methods:The anti-inflammatory effect of the l...Objective:Alchornea floribunda leaves are widely used in ethnomedicinal management of inflammatory disorders. The present work is aimed at investigating this folkloric use.Methods:The anti-inflammatory effect of the leaf extracts and fractions was investigated in experimental animal models of acute and chronic inflammation. The possible mechanisms by which the two most active fractions,hexane(HE) and ethyl acetate(EF) exert their effects were also investigated.Results:The crude extract(200 mg/kg) showed moderate inhibition of egg albumen-induced edema in rats(%edema inhibition = 54.69) at 4 h.HE and EF showed very high activity(%edema inhibition of 81.25 and 67.19 respectively at 200 mg/kg) at 4h as compared to the control. Both fractions ameliorated arthritis induced by formaldehyde in rats.At 400 mg/kg,HE evofeed a significant irritation of gastric mucosa in rats.EF(200 mg/kg,p.o.) significantly inhibited leucocytes(%inhibition = 36.79) migration in vivo,but could not stabilize heat and hypotonicity-indueed lysis of human erythrocyte at 200 and 400μg/mL in vitro.Phytochemical investigation revealed the presence of terpenoids and steroids in HE and flavonoids,tannins and saponins in EF.Conclusion:These results suggest that the leaves of Alchornea floribunda possess anti-inflammatory activity in acute and chronic inflammation.The activity may derive from a combination of inhibition of prostaglandin synthesis and leucocytes migration.The phytochemical constituents detected in HE and EF may account for the anti-inflammatory activity.展开更多
Alchornea cordifolia is a medicinal plant, whose ethanolic and methanolic extracts have shown antioxidant activity which could confer hepatoprotective effect, knowing that liver cells are attacked by free radicals. Th...Alchornea cordifolia is a medicinal plant, whose ethanolic and methanolic extracts have shown antioxidant activity which could confer hepatoprotective effect, knowing that liver cells are attacked by free radicals. The hepatoprotective effect of these extracts has been demonstrated in models of hepatotoxicity induced by paracetamol high doses in animals. However, anti-tubercular drugs at the usual dose present hepatotoxicity risk. Could Alchornea cordifolia help to limit hepatotoxicity induced by anti-tubercular drugs? This work aimed to evaluate the antioxidant activity and the hepatoprotective effect of an aqueous extract of A. cordifolia leaves (AEAC). The antioxidant activity of A. cordifolia leaves was studied in vitro by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals scavenging assay and by the iron reduction ability. A phytochemical screening was carried out to identify the chemical groups that could be responsible for this activity. The hepatoprotective effect was demonstrated in a model of hepatotoxicity induced by isoniazid and rifampicin in rats. Two hours after induction of hepatotoxicity, the animals were orally administered the AEAC at 200 mg/kg, 400 mg/kg, 800 mg/kg for 10 consecutive days. A blood sample was taken on the 11th day for the evaluation of transaminases, markers of hepatic cytolysis. A totally of 96 rats were used in this study. AEAC showed dose-dependent antioxidant activity. Phytochemical screening revealed the presence of flavonoids, tannins and alkaloids. Administrated alone, aqueous extract of A. cordifolia leaves didn’t modificate the transaminases, isoniazid and the isoniazid + rifampicin combination resulted in increasing transaminases (ALT and AST) by more than 48%. AEAC at 800 mg/kg reduced AST and ALT levels by more than 45%. AEAC at 200 mg/kg and 400 mg/kg decreased ALT more than 40%. Knowing that antioxidant activity protects liver, the AEAC may by its antioxidant activity, contribute to protect against the hepatotoxicity induced by anti-tubercular drugs in the rat.展开更多
Alchornea cordifolia (Euphorbiaceae) is a very prized plant among traditional healers in Africa. Its leaves are used for its antipyretic properties in traditional areas. The aim of our study is to determine the acute ...Alchornea cordifolia (Euphorbiaceae) is a very prized plant among traditional healers in Africa. Its leaves are used for its antipyretic properties in traditional areas. The aim of our study is to determine the acute toxicity and the antipyretic activity of a methanolic extract of Alchornea cordifolia leaves. Acute toxicity was assessed by measuring mortality, changes in body weight, spontaneous movements, and normal rectal temperature in mice. Antipyretic activity was evaluated by brewer’s yeast-induced hyperpyrexia in rats according to Teotino method (1963). The antipyretic effect of methanolicextract of Alchornea cordifolia leaves was compared with paracetamol (100 mg/kg bw) orally. Groups of mice treated with doses of 6500;3250;1625 and 812.5mg/kg of the extract did not show any mortality, nor significant alteration of body weight, nor alteration of spontaneous movements. However, incomplete reversed dose-dependent hypothermic activity was observed with doses of 50.78;101.56;203.12;406.25;and 812.5 mg/kg p.o. of the extract, showing acute toxicity of this plant. In the antipyretic assay, the extract with doses of 50.78;101.56;203.12;406.25;and 812.5 mg/kg p.o. exhibited a significant dose-dependent antipyretic activity similar to paracetamol (100 mg/kg bw) in rats. Thus Alchornea cordifolia may inhibit prostaglandins-biosynthesis from hypothalamus. Our results support claims on its traditional uses in management of fever. However Alchornea cordifolia may affect hypothalamus not only during fever but also when body temperature is normal.展开更多
基金supported by the National Natural Science Foundation (No. 39970083).
文摘A new flavonol glycoside, isorhamnetin-3-O-b-D-xyloside, was isolated from the extract of leaves and twigs of Alchornea davidii (Euphorbiaceae). Its structure was established on the basis of the spectral analysis and chemical degradation. Antimicrobial assay showed that it moderately inhibited the growth of test bacteria (Staphylococcus aureus, Bacillus subtilis and Pseudomonas fluorescens) and fungi (Candida albicans, Aspergillus niger and Trichophyton rubrum) with MICs at 50 g/mL.
文摘Objective:To investigate the hepatoprotective and antioxidant activities of Alchornea cordifolia (A.cordifolia) leaf extract.Methods:Various solvent fractions of the methanol extract of the leaf of the plant.4.cordifolia Mull.Arg(Fam:Euphorbiaceae) were evaluated for hepatoprotective activity by carbon tetrachloride-induced liver damage in rats.The degree of protection was measured by using biochemical parameters such as serum glutamate oxalate transaminase(SGOT/ AST),serum glutamate pyruvate transaminase(SCPT/ALT),alkaline phosphatase(ALP) and total bilirubin.The in vitro antioxidant activity of the extract was also evaluated by the I,I-diphenyl 2-picrylhydrazyl(DPPH) free radical scavenging assay.The extract was subjected to preliminary phytoehemical screening.Results:The ethyl acetate and chloroform fractions,at a dose of 300 mg/kg,produced significant(P【0.05) hepatoprolection by decreasing the activities of the serum enzymes and bilirubin while there were marked scavenging of the DPPH free radicals by the fractions.The effects were comparable to those of the standard drugs used for the respective experiments,silymarin and ascorbic acid.Alkaloids,flavonoids,saponins and tannins were delected in the phylochemical screening.Conclusion:From this study,it was concluded that the plant of A.cordifolia possesses hepatoprotective as well as antioxidant activities and these activities reside mainly in the ethyl acetate and acetone fractions of methanol leaf extract.
文摘Objective:Alchornea floribunda leaves are widely used in ethnomedicinal management of inflammatory disorders. The present work is aimed at investigating this folkloric use.Methods:The anti-inflammatory effect of the leaf extracts and fractions was investigated in experimental animal models of acute and chronic inflammation. The possible mechanisms by which the two most active fractions,hexane(HE) and ethyl acetate(EF) exert their effects were also investigated.Results:The crude extract(200 mg/kg) showed moderate inhibition of egg albumen-induced edema in rats(%edema inhibition = 54.69) at 4 h.HE and EF showed very high activity(%edema inhibition of 81.25 and 67.19 respectively at 200 mg/kg) at 4h as compared to the control. Both fractions ameliorated arthritis induced by formaldehyde in rats.At 400 mg/kg,HE evofeed a significant irritation of gastric mucosa in rats.EF(200 mg/kg,p.o.) significantly inhibited leucocytes(%inhibition = 36.79) migration in vivo,but could not stabilize heat and hypotonicity-indueed lysis of human erythrocyte at 200 and 400μg/mL in vitro.Phytochemical investigation revealed the presence of terpenoids and steroids in HE and flavonoids,tannins and saponins in EF.Conclusion:These results suggest that the leaves of Alchornea floribunda possess anti-inflammatory activity in acute and chronic inflammation.The activity may derive from a combination of inhibition of prostaglandin synthesis and leucocytes migration.The phytochemical constituents detected in HE and EF may account for the anti-inflammatory activity.
文摘Alchornea cordifolia is a medicinal plant, whose ethanolic and methanolic extracts have shown antioxidant activity which could confer hepatoprotective effect, knowing that liver cells are attacked by free radicals. The hepatoprotective effect of these extracts has been demonstrated in models of hepatotoxicity induced by paracetamol high doses in animals. However, anti-tubercular drugs at the usual dose present hepatotoxicity risk. Could Alchornea cordifolia help to limit hepatotoxicity induced by anti-tubercular drugs? This work aimed to evaluate the antioxidant activity and the hepatoprotective effect of an aqueous extract of A. cordifolia leaves (AEAC). The antioxidant activity of A. cordifolia leaves was studied in vitro by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals scavenging assay and by the iron reduction ability. A phytochemical screening was carried out to identify the chemical groups that could be responsible for this activity. The hepatoprotective effect was demonstrated in a model of hepatotoxicity induced by isoniazid and rifampicin in rats. Two hours after induction of hepatotoxicity, the animals were orally administered the AEAC at 200 mg/kg, 400 mg/kg, 800 mg/kg for 10 consecutive days. A blood sample was taken on the 11th day for the evaluation of transaminases, markers of hepatic cytolysis. A totally of 96 rats were used in this study. AEAC showed dose-dependent antioxidant activity. Phytochemical screening revealed the presence of flavonoids, tannins and alkaloids. Administrated alone, aqueous extract of A. cordifolia leaves didn’t modificate the transaminases, isoniazid and the isoniazid + rifampicin combination resulted in increasing transaminases (ALT and AST) by more than 48%. AEAC at 800 mg/kg reduced AST and ALT levels by more than 45%. AEAC at 200 mg/kg and 400 mg/kg decreased ALT more than 40%. Knowing that antioxidant activity protects liver, the AEAC may by its antioxidant activity, contribute to protect against the hepatotoxicity induced by anti-tubercular drugs in the rat.
文摘Alchornea cordifolia (Euphorbiaceae) is a very prized plant among traditional healers in Africa. Its leaves are used for its antipyretic properties in traditional areas. The aim of our study is to determine the acute toxicity and the antipyretic activity of a methanolic extract of Alchornea cordifolia leaves. Acute toxicity was assessed by measuring mortality, changes in body weight, spontaneous movements, and normal rectal temperature in mice. Antipyretic activity was evaluated by brewer’s yeast-induced hyperpyrexia in rats according to Teotino method (1963). The antipyretic effect of methanolicextract of Alchornea cordifolia leaves was compared with paracetamol (100 mg/kg bw) orally. Groups of mice treated with doses of 6500;3250;1625 and 812.5mg/kg of the extract did not show any mortality, nor significant alteration of body weight, nor alteration of spontaneous movements. However, incomplete reversed dose-dependent hypothermic activity was observed with doses of 50.78;101.56;203.12;406.25;and 812.5 mg/kg p.o. of the extract, showing acute toxicity of this plant. In the antipyretic assay, the extract with doses of 50.78;101.56;203.12;406.25;and 812.5 mg/kg p.o. exhibited a significant dose-dependent antipyretic activity similar to paracetamol (100 mg/kg bw) in rats. Thus Alchornea cordifolia may inhibit prostaglandins-biosynthesis from hypothalamus. Our results support claims on its traditional uses in management of fever. However Alchornea cordifolia may affect hypothalamus not only during fever but also when body temperature is normal.
