[Objectives]To study the anti-inflammatory effect of Laggerae Alatae Herba extract and its mechanism.[Methods]Inflammation models of xylene-induced ear edema in mice,acetic acid-induced increased permeability of abdom...[Objectives]To study the anti-inflammatory effect of Laggerae Alatae Herba extract and its mechanism.[Methods]Inflammation models of xylene-induced ear edema in mice,acetic acid-induced increased permeability of abdominal capillaries in mice,and carrageenan-induced paw edema in mice were established;xylene-induced ear swelling model in bilateral adrenalectomized mice was established.The levels of MDA,NO and SOD in inflammatory tissues of paw were measured.[Results]Compared with the model group,the high and medium dose groups of Laggerae Alatae Herba extract had significant inhibitory effect on xylene-induced ear edema in mice,except for the low dose group(P>0.05);Laggerae Alatae Herba extract inhibited the increase of celiac capillary permeability induced by acetic acid and paw edema induced by carrageenan in mice.Compared with the model group,in the mice model with bilateral adrenal glands removed,the high and medium dose groups of Laggerae Alatae Herba extract could significantly inhibit the xylene induced ear swelling of the mice.The high and medium dose groups of Laggerae Alatae Herba extract could significantly decrease the levels of MDA and NO,and significantly increase the level of SOD in the paw tissue.[Conclusions]The Laggerae Alatae Herba extracts have anti-inflammatory activity,and the anti-inflammatory effect of the extracts does not depend on the hypothalamic-pituitary-adrenal axis(HPAA)system.In addition,the anti-inflammatory mechanism of Laggerae Alatae Herba extract is related to the decrease of MDA and NO and the increase of SOD.展开更多
[Objectives]Taking chlorogenic acid as index component,TLC and content determination method of Laggerae Alatae Herba were established.[Methods]TLC identification used silica gel G thin-layer plate,and butyl acetate∶f...[Objectives]Taking chlorogenic acid as index component,TLC and content determination method of Laggerae Alatae Herba were established.[Methods]TLC identification used silica gel G thin-layer plate,and butyl acetate∶formic acid∶water(7∶2.5∶2.5)was taken as developing agent,and it was inspected under ultraviolet lamp(365 nm).The content was determined by chromatographic column Inertsil ODS-3 C_(18)(4.60 mm×250 mm,5μm).Mobile phase:methanol-0.1%phosphoric acid(28∶72);detection wavelength:329 nm;flow speed:1.0 mL/min;column temperature:25℃;injection volume:10μL.[Results]Chlorogenic acid can be detected by TLC,with clear spots and good specificity.When injection volume of chlorogenic acid was between 0.099 and 0.990μg(R^(2)=0.9999),there was good linear relationship.In low,medium and high sample adding groups of Laggerae Alatae Herba,average recovery rates of chlorogenic acids were 98.80%(RSD=2.09%),98.24%(RSD=1.96%)and 99.65%(RSD=2.15%).[Conclusions]The method could effectively identify medicinal material Laggerae Alatae Herba,and accurately measure the content of chlorogenic acid in Laggerae Alatae Herba,thereby providing a scientific basis for developing and using medicinal resources of Laggerae Alatae Herba.展开更多
[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guan...[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guangxi,so as to provide a simple and rapid identification method for the identification of H.auricularia L.in Guangxi.[Methods]LMVP(leaf morphology-venation pattern)identification method was used to study the characteristics of leaf morphology-venation pattern.[Results]Characteristics of leaf morphology-venation pattern:(i)H.auricularia L.:circular knotted-curved pinnate leaf venation without reaching the margin;the primary veins run straight without branches,the number of secondary veins is 5 to 9,and the angles included in tertiary veins are mostly near right angles or obtuse angles.(ii)M.hirtus(L.)DC.:curved pinnate leaf venation without reaching the margin;the primary veins run straight without obvious changes,the number of secondary veins is 3 to 5,and the tertiary veins are slender,and the included angles are mostly acute.(iii)S.pusilla Wall.:curved leaf venation without reaching the leaf margin;there are 4 to 5 pairs of secondary veins,most of which are opposite;the angle between the secondary veins and the primary veins in the middle and near the petiole is mostly medium acute angle,and the extension of the tertiary veins has no fixed direction.(iv)S.alata Aubl.:circular knotted-curved pinnate leaf venation;the primary veins are convex and straight without branches,and there are 4 to 6 pairs of secondary veins,alternating.The angle between secondary veins and primary veins is mostly medium acute angle,the angle between tertiary veins and secondary veins is near right angle,and the tertiary veins are mostly transversely extended.[Conclusions]The leaf morphology-venation patterns of the above-mentioned H.auricularia L.and M.hirtus(L.)DC.,S.pusilla Wall.,and S.alata Aubl.can be used as their identification features,and the identification method has certain operability,which provides an identification idea for the identification of Chinese herbal medicines.展开更多
[Objective] This study was to explore the effect of purple yam on the nutritional and physiological functions of rats.[Method] Forty SD rats were randomly divided into four groups with ten rats in each group which wer...[Objective] This study was to explore the effect of purple yam on the nutritional and physiological functions of rats.[Method] Forty SD rats were randomly divided into four groups with ten rats in each group which were reared with artificial semisynthetic feed added with 10% steamed yam powder.The four groups,the casein（CK） group which was used as the control,the purple yam（PY） group,the Tiegun yam（TY） group and common Huai yam（HY） group,were supplied with feed of equal content of energy,protein and fat.The rats were pair-fed for 56 days to observe changes of relevant nutritional and physiological indices.[Result] There were no significant differences of body weight,food conversion rate and organ indices between rats in PY group and CK group.Mean corpuscular hemoglobin in PY group was significantly lower than that in CK group;hemoglobin showed no significant difference with that in CK group;eosnophils was lower than that in CK group.Low density lipoprotein-cholesterol,total cholesterol and atherogenic index in PY group were lower than that in the other three groups;anti-atherogenic index was the highest among the four groups,while showing no significant difference;triglyceride content was lower than that in CK group.Among the antioxidant indices,the activity of catalase and superoxide dismutase were higher than that in CK group,while malondialdehyde content was lower than that in CK group.[Conclusion] Purple yam showed the trend of reducing the content of blood glucose,total cholesterol,low density lipoprotein-cholesterol,triglyceride,malondialdehyde,and raising the activity of glutathione peroxidase,superoxide dismutase and catalase,thus having certain antioxidative function.展开更多
A cruise was conducted from late August to early September 2004 with the intention of obtaining an interdisciplinary understanding of the Yangtze River Estuary including the biological, chemical and physical subjects....A cruise was conducted from late August to early September 2004 with the intention of obtaining an interdisciplinary understanding of the Yangtze River Estuary including the biological, chemical and physical subjects. Water sample analysis indicated that total phytoplankton species richness was 137. Of them 81 were found in Bacillariophyta and 48 in Pyrrophyta, accounting for 59.1% and 35.0% respectively. The average cell abundance of surface water samples was 8.8 ×104 cells L^-1, with the maximum, 102.9×104 cells L^-1, encountered in the area （31.75°N, 122.33°E） and the minimum, 0.2×10^4 cells L^-1, in （30.75°N, 122.17°E）. The dominant species at most stations were Skeletonema costatum and Proboscia alata f. gracillima with the dominance of 0.35 and 0.27. Vertical distribution analysis indicated that obvious stratification of cell abundance and dominant species was found in the representative stations of 5, 18 and 33. Shannon-Wiener index and evenness of phytoplankton assemblage presented negative correlation with the cell abundance, with the optimum appearing in （30.75°N, 122.67°E）. According to the PCA analysis of the environmental variables, elevated nutrients of nitrate, silicate and phosphate through river discharge were mainly responsible for the phytoplankton bloom in this area.展开更多
Unidirectional liquid transport without any need of external energy has drawn worldwide attention for its potential applications in various fields such as microfluidics,biomedicine and mechanical engineering.In nature...Unidirectional liquid transport without any need of external energy has drawn worldwide attention for its potential applications in various fields such as microfluidics,biomedicine and mechanical engineering.In nature,numerous creatures have evolved such extraordinary unidirectional liquid transport ability such as spider sik,Sarracenia's trichomes,and Nepenthes alata's peristome,etc.This review summarizes the current progresses of natural unidirectional liquid transport on 1-Dimensional(1D)linear structure and 2-Dimensional(2D)surface stucture.The driving force of unidirectional liquid transport which is determined by unique structure exist distinct differences in physics.The fundamental understanding of 1D and 2D unidirectionaliquid transport especially about hierarchical structural characteristics and their transport mechanism were concentrated,and various bioinspired fabrication methods are also introduced.The applications of bioinspired directional liquid transport are demonstrated especially in fields of microfluidies,biomedical devices and anti-icing surfaces.With newly developed smart materials,various liquid transport regulation strategies are also summarized for the control of transport speed,direction guiding,etc.Finally,we provide new insights and future perspectives of the directional transport materials.展开更多
基金Supported by State Administration of Traditional Chinese Medicine High-level Key Discipline Construction Project of Traditional Chinese Medicine-Ethnic Minority Pharmacy (Zhuang Pharmacy) (zyyzdxk-2023165)Cultivation Project of Guangxi International Zhuang Medicine Hospital (2023GZYJKT008)+6 种基金Youth Fund of Natural Science Foundation of Guangxi (2024GXNSFBA010302)Young Talent Cultivation Program of Guangxi International Zhuang Medicine Hospital (2022001)Key R&D Project of Guangxi Science and Technology Department (Guike AB21196057)Guangxi Traditional Chinese Medicine Interdisciplinary Innovation Team Project (GZKJ2309)Funding Project of High-level Talent Cultivation and Innovation Team of Guangxi University of Chinese Medicine (2022A008)The Third Batch of"Qihuang Project"High-Level Talent Team Training Project of Guangxi University of Chinese Medicine (202414)Three-year Action Plan for the Construction of High-level Talents Team of Guangxi International Zhuang Medicine Hospital in 2023 (GZCX20231203).
文摘[Objectives]To study the anti-inflammatory effect of Laggerae Alatae Herba extract and its mechanism.[Methods]Inflammation models of xylene-induced ear edema in mice,acetic acid-induced increased permeability of abdominal capillaries in mice,and carrageenan-induced paw edema in mice were established;xylene-induced ear swelling model in bilateral adrenalectomized mice was established.The levels of MDA,NO and SOD in inflammatory tissues of paw were measured.[Results]Compared with the model group,the high and medium dose groups of Laggerae Alatae Herba extract had significant inhibitory effect on xylene-induced ear edema in mice,except for the low dose group(P>0.05);Laggerae Alatae Herba extract inhibited the increase of celiac capillary permeability induced by acetic acid and paw edema induced by carrageenan in mice.Compared with the model group,in the mice model with bilateral adrenal glands removed,the high and medium dose groups of Laggerae Alatae Herba extract could significantly inhibit the xylene induced ear swelling of the mice.The high and medium dose groups of Laggerae Alatae Herba extract could significantly decrease the levels of MDA and NO,and significantly increase the level of SOD in the paw tissue.[Conclusions]The Laggerae Alatae Herba extracts have anti-inflammatory activity,and the anti-inflammatory effect of the extracts does not depend on the hypothalamic-pituitary-adrenal axis(HPAA)system.In addition,the anti-inflammatory mechanism of Laggerae Alatae Herba extract is related to the decrease of MDA and NO and the increase of SOD.
基金Supported by Key R&D Projects of Guangxi Science and Technology Department (GUIKE AB21196057)Basic Scientific Research Ability Improvement Project of Young and Middle-aged Teachers in Guangxi Universities and Colleges in 2019 (2019KY0341)+1 种基金Open Project of Guangxi Zhuang Yao Medicine Key Laboratory (GXZYKF2020A-08)Zhuang Pharmacy,a Key Discipline of Traditional Chinese Medicine (Ethnic Pharmacy) in the"12^th Five-year" Plan of the State Administration of Traditional Chinese Medicine
文摘[Objectives]Taking chlorogenic acid as index component,TLC and content determination method of Laggerae Alatae Herba were established.[Methods]TLC identification used silica gel G thin-layer plate,and butyl acetate∶formic acid∶water(7∶2.5∶2.5)was taken as developing agent,and it was inspected under ultraviolet lamp(365 nm).The content was determined by chromatographic column Inertsil ODS-3 C_(18)(4.60 mm×250 mm,5μm).Mobile phase:methanol-0.1%phosphoric acid(28∶72);detection wavelength:329 nm;flow speed:1.0 mL/min;column temperature:25℃;injection volume:10μL.[Results]Chlorogenic acid can be detected by TLC,with clear spots and good specificity.When injection volume of chlorogenic acid was between 0.099 and 0.990μg(R^(2)=0.9999),there was good linear relationship.In low,medium and high sample adding groups of Laggerae Alatae Herba,average recovery rates of chlorogenic acids were 98.80%(RSD=2.09%),98.24%(RSD=1.96%)and 99.65%(RSD=2.15%).[Conclusions]The method could effectively identify medicinal material Laggerae Alatae Herba,and accurately measure the content of chlorogenic acid in Laggerae Alatae Herba,thereby providing a scientific basis for developing and using medicinal resources of Laggerae Alatae Herba.
基金Supported by Guangxi Key Laboratory of Zhuang and Yao Medicine(GuiKeJiZi 201432)Zhuang and Yao Medicine Collaborative Innovation Center(GuiJiaoKeYan 201320)+3 种基金Guangxi Young Talent Project of Guangxi University of Chinese Medicine(2022C030)Ethnic Medicine Resources and Application Engineering Research Center in Guangxi Zhuang Autonomous Region(GuiFaGaiGaoJiHan 20202605)Key Discipline Project of Traditional Chinese Medicine in Guangxi—Zhuang Medicine(GZXK-Z-20-64)Guangxi First-class Discipline of Traditional Chinese Medicine—Ethnopharmacology(GuiJiaoKeYan 201812).
文摘[Objectives]To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L.and its adulterants,such as Mitracarpus hirtus(L.)DC.,Spermacoce pusilla Wall.and Spermacoce alata Aubl.,produced in Guangxi,so as to provide a simple and rapid identification method for the identification of H.auricularia L.in Guangxi.[Methods]LMVP(leaf morphology-venation pattern)identification method was used to study the characteristics of leaf morphology-venation pattern.[Results]Characteristics of leaf morphology-venation pattern:(i)H.auricularia L.:circular knotted-curved pinnate leaf venation without reaching the margin;the primary veins run straight without branches,the number of secondary veins is 5 to 9,and the angles included in tertiary veins are mostly near right angles or obtuse angles.(ii)M.hirtus(L.)DC.:curved pinnate leaf venation without reaching the margin;the primary veins run straight without obvious changes,the number of secondary veins is 3 to 5,and the tertiary veins are slender,and the included angles are mostly acute.(iii)S.pusilla Wall.:curved leaf venation without reaching the leaf margin;there are 4 to 5 pairs of secondary veins,most of which are opposite;the angle between the secondary veins and the primary veins in the middle and near the petiole is mostly medium acute angle,and the extension of the tertiary veins has no fixed direction.(iv)S.alata Aubl.:circular knotted-curved pinnate leaf venation;the primary veins are convex and straight without branches,and there are 4 to 6 pairs of secondary veins,alternating.The angle between secondary veins and primary veins is mostly medium acute angle,the angle between tertiary veins and secondary veins is near right angle,and the tertiary veins are mostly transversely extended.[Conclusions]The leaf morphology-venation patterns of the above-mentioned H.auricularia L.and M.hirtus(L.)DC.,S.pusilla Wall.,and S.alata Aubl.can be used as their identification features,and the identification method has certain operability,which provides an identification idea for the identification of Chinese herbal medicines.
基金Natural Science Foundation of Guangxi Academy of Agricultural Sciences(No.2003014 and 2009014)The Project ofSpecial Funds for the Employment of Outstanding Experts in Guangxi
基金Supported by the Special Fund for Construction of Scientific and Technological Innovation Ability of BAAFS(KJCX201101010-22)~~
文摘[Objective] This study was to explore the effect of purple yam on the nutritional and physiological functions of rats.[Method] Forty SD rats were randomly divided into four groups with ten rats in each group which were reared with artificial semisynthetic feed added with 10% steamed yam powder.The four groups,the casein（CK） group which was used as the control,the purple yam（PY） group,the Tiegun yam（TY） group and common Huai yam（HY） group,were supplied with feed of equal content of energy,protein and fat.The rats were pair-fed for 56 days to observe changes of relevant nutritional and physiological indices.[Result] There were no significant differences of body weight,food conversion rate and organ indices between rats in PY group and CK group.Mean corpuscular hemoglobin in PY group was significantly lower than that in CK group;hemoglobin showed no significant difference with that in CK group;eosnophils was lower than that in CK group.Low density lipoprotein-cholesterol,total cholesterol and atherogenic index in PY group were lower than that in the other three groups;anti-atherogenic index was the highest among the four groups,while showing no significant difference;triglyceride content was lower than that in CK group.Among the antioxidant indices,the activity of catalase and superoxide dismutase were higher than that in CK group,while malondialdehyde content was lower than that in CK group.[Conclusion] Purple yam showed the trend of reducing the content of blood glucose,total cholesterol,low density lipoprotein-cholesterol,triglyceride,malondialdehyde,and raising the activity of glutathione peroxidase,superoxide dismutase and catalase,thus having certain antioxidative function.
基金This study was supported by the National Natural Science Foundation of China(Grant Nos.50339040,40306025)the Chinese Academy of Sciences(No.KZCX3-SW-232).The authors are also very much indebted to the crews of the cruise.
文摘A cruise was conducted from late August to early September 2004 with the intention of obtaining an interdisciplinary understanding of the Yangtze River Estuary including the biological, chemical and physical subjects. Water sample analysis indicated that total phytoplankton species richness was 137. Of them 81 were found in Bacillariophyta and 48 in Pyrrophyta, accounting for 59.1% and 35.0% respectively. The average cell abundance of surface water samples was 8.8 ×104 cells L^-1, with the maximum, 102.9×104 cells L^-1, encountered in the area （31.75°N, 122.33°E） and the minimum, 0.2×10^4 cells L^-1, in （30.75°N, 122.17°E）. The dominant species at most stations were Skeletonema costatum and Proboscia alata f. gracillima with the dominance of 0.35 and 0.27. Vertical distribution analysis indicated that obvious stratification of cell abundance and dominant species was found in the representative stations of 5, 18 and 33. Shannon-Wiener index and evenness of phytoplankton assemblage presented negative correlation with the cell abundance, with the optimum appearing in （30.75°N, 122.67°E）. According to the PCA analysis of the environmental variables, elevated nutrients of nitrate, silicate and phosphate through river discharge were mainly responsible for the phytoplankton bloom in this area.
基金the National Key R&D Program of China(No.2019YFB1309702)the National Natural Science Foundation of China(Nos.51935001,51725501 and 51905022).
文摘Unidirectional liquid transport without any need of external energy has drawn worldwide attention for its potential applications in various fields such as microfluidics,biomedicine and mechanical engineering.In nature,numerous creatures have evolved such extraordinary unidirectional liquid transport ability such as spider sik,Sarracenia's trichomes,and Nepenthes alata's peristome,etc.This review summarizes the current progresses of natural unidirectional liquid transport on 1-Dimensional(1D)linear structure and 2-Dimensional(2D)surface stucture.The driving force of unidirectional liquid transport which is determined by unique structure exist distinct differences in physics.The fundamental understanding of 1D and 2D unidirectionaliquid transport especially about hierarchical structural characteristics and their transport mechanism were concentrated,and various bioinspired fabrication methods are also introduced.The applications of bioinspired directional liquid transport are demonstrated especially in fields of microfluidies,biomedical devices and anti-icing surfaces.With newly developed smart materials,various liquid transport regulation strategies are also summarized for the control of transport speed,direction guiding,etc.Finally,we provide new insights and future perspectives of the directional transport materials.
