The present study was to evaluate thyme and ajwain as antibiotic growth promoter substitutions on growth performance,carcass characteristics and serum biochemistry in Japanese quails(Coturnix japonica).A feeding trial...The present study was to evaluate thyme and ajwain as antibiotic growth promoter substitutions on growth performance,carcass characteristics and serum biochemistry in Japanese quails(Coturnix japonica).A feeding trial was conducted over 28 d with 4 groups of Japanese quails fed experimental diets formulated to provide no supplementation(control),or control t 55 mg zinc bacitracin/kg,2 g thyme/kg,and 2 g ajwain/kg.At 35 d of age,2 quails from each replicate were sacrificed and eviscerated.Liver,empty small intestine and heart were weighed and calculated as a percentage of live body weight.The carcasses were weighed and the cuts were performed to evaluate the yield of legs and breast.At 35 d of age,2 quails per replicate were chosen and approximately 1-m L blood samples were collected through brachial vein,and concentrations of albumin,total protein,triglyceride,total cholesterol,and high density lipoprotein(HDL)-cholesterol were determined.Final body weight were not affected by the dietary treatment whereas,it tended to increase in quails supplemented with antibiotic.Overall feed conversation ratio values were similar though it tended to improve in quails supplemented with thyme compared with other groups.Daily feed intake,internal organ weights and carcass traits were not influenced by the dietary treatments.Serum total cholesterol contents were lower(P < 0.05) in quails supplemented with ajwain compared with control and quails supplemented with antibiotic and thyme.The highest serum HDL-cholesterol was seen in the group supplemented with thyme compared with other groups.It was concluded that the addition of 2 g/kg thyme can improve serum biochemistry in quails,although its effects on performance criteria was negligible.展开更多
Trachyspermum ammi(L.),Carum copticum,commonly known as Ajwain(Apiaceae),native to Egypt;is known to possess different biological activities.It contains mainly thymol derivatives.Plant tissue culture provides a contro...Trachyspermum ammi(L.),Carum copticum,commonly known as Ajwain(Apiaceae),native to Egypt;is known to possess different biological activities.It contains mainly thymol derivatives.Plant tissue culture provides a controlled supply of biochemicals.The objective is to study the effect of sprouting on the bioactive metabolites of T.ammi(L.).Ajwain fruits were divided into three groups;the first and the second groups were sprouted over a piece of cotton and over Murashige&Skoog’s medium under aseptic conditions.The resultant sprouts were extracted separately using alcohol.The third group proceeded by culturing the green buds over the MS supplemented with phytohormones to initiate callus.The obtained samples analyzed using UPLC-MS-LTQ-Orbitrap in negative ion mode.Thirty-one peaks of different classes(flavonoids,caffeoyl quinic acids,coumarins and thymol derivatives)were putatively identified.Some valuable components such as furanochromones(visamminol,praeroside),dicaffeoylquinic acid,rosmarinic and feruloylquinic acids,and sinapic acid were detected for the first time in this species.Furthermore,glycosyringic acid was detected for the first time in the species T.ammi and in the family Apiaceae.UPLC-MS data were subjected to unsupervised(heatmap,PCA)and supervised(PLS-DA)models to gain a comprehensive understanding of both the quantitative and qualitative aspects of clustering tendencies in the samples The first model consistently clustered the five samples into three groups then the latter model demonstrated strong predictive power for unseen testing data and provided key insights into twelve peaks that may serve as potential chemical markers for distinguishing the five samples.展开更多
基金supported by Islamic Azad University,Shahrekord Branch (grant number:2014/04)
文摘The present study was to evaluate thyme and ajwain as antibiotic growth promoter substitutions on growth performance,carcass characteristics and serum biochemistry in Japanese quails(Coturnix japonica).A feeding trial was conducted over 28 d with 4 groups of Japanese quails fed experimental diets formulated to provide no supplementation(control),or control t 55 mg zinc bacitracin/kg,2 g thyme/kg,and 2 g ajwain/kg.At 35 d of age,2 quails from each replicate were sacrificed and eviscerated.Liver,empty small intestine and heart were weighed and calculated as a percentage of live body weight.The carcasses were weighed and the cuts were performed to evaluate the yield of legs and breast.At 35 d of age,2 quails per replicate were chosen and approximately 1-m L blood samples were collected through brachial vein,and concentrations of albumin,total protein,triglyceride,total cholesterol,and high density lipoprotein(HDL)-cholesterol were determined.Final body weight were not affected by the dietary treatment whereas,it tended to increase in quails supplemented with antibiotic.Overall feed conversation ratio values were similar though it tended to improve in quails supplemented with thyme compared with other groups.Daily feed intake,internal organ weights and carcass traits were not influenced by the dietary treatments.Serum total cholesterol contents were lower(P < 0.05) in quails supplemented with ajwain compared with control and quails supplemented with antibiotic and thyme.The highest serum HDL-cholesterol was seen in the group supplemented with thyme compared with other groups.It was concluded that the addition of 2 g/kg thyme can improve serum biochemistry in quails,although its effects on performance criteria was negligible.
文摘Trachyspermum ammi(L.),Carum copticum,commonly known as Ajwain(Apiaceae),native to Egypt;is known to possess different biological activities.It contains mainly thymol derivatives.Plant tissue culture provides a controlled supply of biochemicals.The objective is to study the effect of sprouting on the bioactive metabolites of T.ammi(L.).Ajwain fruits were divided into three groups;the first and the second groups were sprouted over a piece of cotton and over Murashige&Skoog’s medium under aseptic conditions.The resultant sprouts were extracted separately using alcohol.The third group proceeded by culturing the green buds over the MS supplemented with phytohormones to initiate callus.The obtained samples analyzed using UPLC-MS-LTQ-Orbitrap in negative ion mode.Thirty-one peaks of different classes(flavonoids,caffeoyl quinic acids,coumarins and thymol derivatives)were putatively identified.Some valuable components such as furanochromones(visamminol,praeroside),dicaffeoylquinic acid,rosmarinic and feruloylquinic acids,and sinapic acid were detected for the first time in this species.Furthermore,glycosyringic acid was detected for the first time in the species T.ammi and in the family Apiaceae.UPLC-MS data were subjected to unsupervised(heatmap,PCA)and supervised(PLS-DA)models to gain a comprehensive understanding of both the quantitative and qualitative aspects of clustering tendencies in the samples The first model consistently clustered the five samples into three groups then the latter model demonstrated strong predictive power for unseen testing data and provided key insights into twelve peaks that may serve as potential chemical markers for distinguishing the five samples.
