Objective:To assess the efficiency of a Sophora flavescens Ait(S.flavescens,Ku Shen)-soluble microneedle(SFA-MN)for improving skin lesion symptoms in mice with psoriasis.Methods:SFA-MNs were prepared using a two-mold ...Objective:To assess the efficiency of a Sophora flavescens Ait(S.flavescens,Ku Shen)-soluble microneedle(SFA-MN)for improving skin lesion symptoms in mice with psoriasis.Methods:SFA-MNs were prepared using a two-mold molding process with 20%w/v poly-vinylpyrrolidone and 15%w/v polyvinyl alcohol.The SFA-MNs were assessed for morphology,mechanical properties,in vitro dissolution,identification of components,and skin lesion improvement in imiquimod-induced psoriasis mice.Results:The SFA-MNs demonstrated good mechanical properties for efficiently penetrating the dermis,facilitating efficient drug delivery.Furthermore,they effectively inhibited mast cell levels in the dorsal lesion area of psoriasis mice and reduced the expression of the T-lymphocyte factor cluster of differ-entiation 3 and tumor necrosis factor-a.In addition,this system alleviated skin inflammation,splenic swelling,and thymic atrophy in the psoriasis-like mouse model.Seven major components were detected from SFA-MNs by comparison of the mass-to-nucleus ratios(m/z)of the secondary fragments N-methylcytisine,5a,9a-dihydroxymatrine,sophoramine,matrine,oxysophocarpine,oxymatrine,and kushenol O.Conclusion:The drug delivery strategy combining traditional herbal S.flavescens with soluble micro-needle technology provides more targeted and effective immune regulation for treating psoriasis-like mice models,enabling enhanced therapeutic effects compared with the control group.展开更多
Background:Euphorbia prostrata Ait.is an annual herb widely distributed in the southern region of China with great medical values on Anti-inflammation,insect repellent,treatment of diarrhea.Despite its extensive uses ...Background:Euphorbia prostrata Ait.is an annual herb widely distributed in the southern region of China with great medical values on Anti-inflammation,insect repellent,treatment of diarrhea.Despite its extensive uses as a traditional Chinese medicine,no systematic research on the identification of E.prostrata has been reported.Methods:The study aimed to establish an accurate identification system for E.prostrata through traditional pharmacognostical methods,including botanical origin,morphological characters,medicinal material characters,microscopic characters,physicochemical parameters determination,phytochemical screening,and DNA barcoding analysis.Results:Physicochemical results show that this plant likely contains flavonoids,anthraquinones,and other substances.The ITS loci of the nuclear genome and psbA-trnH loci of the chloroplast genome were selected and evaluated,which were the most variable loci.Conclusion:The findings of this study are expected to contribute to the development of species identification,as well as provide references for authenticity identification,genetic relationship analysis,and further utilization of E.prostrata.展开更多
[目的]探索化学除草剂对2种中药材苦参和射干苗期杂草的防除效果及其对该2种作物的安全性。[方法]通过田间小区试验,研究40%砜吡草唑SC土壤喷雾+30%苯唑草酮SC播种后21 d茎叶喷雾,按照低(120+22.5)、中(180+27)、高(240+45)g a.i./hm^(...[目的]探索化学除草剂对2种中药材苦参和射干苗期杂草的防除效果及其对该2种作物的安全性。[方法]通过田间小区试验,研究40%砜吡草唑SC土壤喷雾+30%苯唑草酮SC播种后21 d茎叶喷雾,按照低(120+22.5)、中(180+27)、高(240+45)g a.i./hm^(2)3个剂量组合搭配,48%仲丁灵EC按照1440 g a.i./hm^(2)苗前一次性土壤喷雾的方法,施药后持续调查4种不同处理对田间杂草的株防效,以及对苦参和射干2种作物出苗和株高的影响。[结果]40%砜吡草唑SC+30%苯唑草酮SC的中(180+27)、低(120+22.5)g a.i./hm^(2)剂量处理组合在苗后茎叶处理后7~42 d,对田间杂草的株防效可维持在80%以上,且该剂量组合处理对苦参和射干的出苗和株高并无明显不利影响。[结论]选用苗前土壤处理除草剂40%砜吡草唑SC按照120~180 g a.i./hm^(2),配合播种后21 d按照22.5~27 g a.i./hm^(2)施用苗后除草剂30%苯唑草酮SC,对苦参和射干2种作物田杂草的防除效果和安全性良好。展开更多
Leaves from three_year_old solar greenhouse nectarine trees ( Prunus persica L. var. nectarina Ait. “Zao Hong Yan”) were used as materials in this study. It was the first time that the ultrastructural charact...Leaves from three_year_old solar greenhouse nectarine trees ( Prunus persica L. var. nectarina Ait. “Zao Hong Yan”) were used as materials in this study. It was the first time that the ultrastructural characteristics of phloem tissues of source leaves were observed and compared in normal and weak light intensities using the transmission electron microscopy. Results showed that the average diameters of companion cells (CC) and sieve elements (SE) of all kinds of veins were bigger in normal than that in weak light intensity, indicating that light could influence the cell development and growth. Dense cytoplasm with abundant mitochondria, endoplasmic reticulums, multivesicular bodies, vesicles and plastids were observed in normal light intensity. On the contrary, CC with small vacuolar structures and few mitochondrias, endoplasmic reticulums were shown in weak light. Misalignment of grana thylakoid margins of nectarine leaves also was seen in weak light. The sieve pores of SEs were obstructed in weak light. Chloroplasts with numerous starch grains and few mitochondrias were noticed in the mesophyll cell (MES) surrounding the bundle sheath in weak light. The storage of starch grains appeared to result from an unbalance between photosynthate production and export of photosynthates. This observation provided a strong support to the point that most leaves export the most of assimilates in the light time. Plasmodesmal densities between SE/CC, CC/PP (phloem parenchyma cell), PP/PP and PP/BSC (bundle_sheath cell) decreased in weak light. Plasmodesmata were observed between CC/SE (NS) (nacreous_walled sieve element), PP/BSC in branch veins in normal light intensity, but not in weak light. Thus apoplasmic pathway may be the main mode of transport of assimilates in weak light, however symplasmic pathway may be the main mode of transport of assimilates in normal light intensity. These results demonstrated that the solar greenhouse nectarine trees could be adapted to the weak light via the ultrastructure variation of phloem tissues of the source leaves.展开更多
基金supported by the National Natural Science Foundation of China(82274225)NATCM's Project of High-level Construction of Key TCM Disciplines-Beijing University of Chinese Medicine-Life Science from the Perspective of Chinese Medicine(zyyzdxk-2023263).
文摘Objective:To assess the efficiency of a Sophora flavescens Ait(S.flavescens,Ku Shen)-soluble microneedle(SFA-MN)for improving skin lesion symptoms in mice with psoriasis.Methods:SFA-MNs were prepared using a two-mold molding process with 20%w/v poly-vinylpyrrolidone and 15%w/v polyvinyl alcohol.The SFA-MNs were assessed for morphology,mechanical properties,in vitro dissolution,identification of components,and skin lesion improvement in imiquimod-induced psoriasis mice.Results:The SFA-MNs demonstrated good mechanical properties for efficiently penetrating the dermis,facilitating efficient drug delivery.Furthermore,they effectively inhibited mast cell levels in the dorsal lesion area of psoriasis mice and reduced the expression of the T-lymphocyte factor cluster of differ-entiation 3 and tumor necrosis factor-a.In addition,this system alleviated skin inflammation,splenic swelling,and thymic atrophy in the psoriasis-like mouse model.Seven major components were detected from SFA-MNs by comparison of the mass-to-nucleus ratios(m/z)of the secondary fragments N-methylcytisine,5a,9a-dihydroxymatrine,sophoramine,matrine,oxysophocarpine,oxymatrine,and kushenol O.Conclusion:The drug delivery strategy combining traditional herbal S.flavescens with soluble micro-needle technology provides more targeted and effective immune regulation for treating psoriasis-like mice models,enabling enhanced therapeutic effects compared with the control group.
文摘Background:Euphorbia prostrata Ait.is an annual herb widely distributed in the southern region of China with great medical values on Anti-inflammation,insect repellent,treatment of diarrhea.Despite its extensive uses as a traditional Chinese medicine,no systematic research on the identification of E.prostrata has been reported.Methods:The study aimed to establish an accurate identification system for E.prostrata through traditional pharmacognostical methods,including botanical origin,morphological characters,medicinal material characters,microscopic characters,physicochemical parameters determination,phytochemical screening,and DNA barcoding analysis.Results:Physicochemical results show that this plant likely contains flavonoids,anthraquinones,and other substances.The ITS loci of the nuclear genome and psbA-trnH loci of the chloroplast genome were selected and evaluated,which were the most variable loci.Conclusion:The findings of this study are expected to contribute to the development of species identification,as well as provide references for authenticity identification,genetic relationship analysis,and further utilization of E.prostrata.
文摘[目的]探索化学除草剂对2种中药材苦参和射干苗期杂草的防除效果及其对该2种作物的安全性。[方法]通过田间小区试验,研究40%砜吡草唑SC土壤喷雾+30%苯唑草酮SC播种后21 d茎叶喷雾,按照低(120+22.5)、中(180+27)、高(240+45)g a.i./hm^(2)3个剂量组合搭配,48%仲丁灵EC按照1440 g a.i./hm^(2)苗前一次性土壤喷雾的方法,施药后持续调查4种不同处理对田间杂草的株防效,以及对苦参和射干2种作物出苗和株高的影响。[结果]40%砜吡草唑SC+30%苯唑草酮SC的中(180+27)、低(120+22.5)g a.i./hm^(2)剂量处理组合在苗后茎叶处理后7~42 d,对田间杂草的株防效可维持在80%以上,且该剂量组合处理对苦参和射干的出苗和株高并无明显不利影响。[结论]选用苗前土壤处理除草剂40%砜吡草唑SC按照120~180 g a.i./hm^(2),配合播种后21 d按照22.5~27 g a.i./hm^(2)施用苗后除草剂30%苯唑草酮SC,对苦参和射干2种作物田杂草的防除效果和安全性良好。
文摘Leaves from three_year_old solar greenhouse nectarine trees ( Prunus persica L. var. nectarina Ait. “Zao Hong Yan”) were used as materials in this study. It was the first time that the ultrastructural characteristics of phloem tissues of source leaves were observed and compared in normal and weak light intensities using the transmission electron microscopy. Results showed that the average diameters of companion cells (CC) and sieve elements (SE) of all kinds of veins were bigger in normal than that in weak light intensity, indicating that light could influence the cell development and growth. Dense cytoplasm with abundant mitochondria, endoplasmic reticulums, multivesicular bodies, vesicles and plastids were observed in normal light intensity. On the contrary, CC with small vacuolar structures and few mitochondrias, endoplasmic reticulums were shown in weak light. Misalignment of grana thylakoid margins of nectarine leaves also was seen in weak light. The sieve pores of SEs were obstructed in weak light. Chloroplasts with numerous starch grains and few mitochondrias were noticed in the mesophyll cell (MES) surrounding the bundle sheath in weak light. The storage of starch grains appeared to result from an unbalance between photosynthate production and export of photosynthates. This observation provided a strong support to the point that most leaves export the most of assimilates in the light time. Plasmodesmal densities between SE/CC, CC/PP (phloem parenchyma cell), PP/PP and PP/BSC (bundle_sheath cell) decreased in weak light. Plasmodesmata were observed between CC/SE (NS) (nacreous_walled sieve element), PP/BSC in branch veins in normal light intensity, but not in weak light. Thus apoplasmic pathway may be the main mode of transport of assimilates in weak light, however symplasmic pathway may be the main mode of transport of assimilates in normal light intensity. These results demonstrated that the solar greenhouse nectarine trees could be adapted to the weak light via the ultrastructure variation of phloem tissues of the source leaves.
