This study is to assess the biotechnological potential of agro-industrial wastes of pineapple(Ananas comosus),sweet potato(Ipomoea batatas)and watermelon(Citrullus lanatus)for protein enrichment by solid-state ferment...This study is to assess the biotechnological potential of agro-industrial wastes of pineapple(Ananas comosus),sweet potato(Ipomoea batatas)and watermelon(Citrullus lanatus)for protein enrichment by solid-state fermentation using Aspergillus niger.Spore suspensions of A.niger were prepared with potato dextrose broth that had been supplemented with sodium chloride,ammonium nitrate and thiamine,and adjusted to pH of 5.Initial protein contents of sterile and non-sterile wastes of the pineapple,sweet potato,and watermelon were determined by the Kjeldahl method.The remaining sterile and non-sterile wastes was inoculated with spore suspensions of A.niger and incubated at temperature of 24℃ under conditions of solid-state fermentation for 14 days.Protein contents of the inoculated sterile and non-sterile wastes were determined after 7 and 14 days.The sterile wastes of pineapple,sweet potato and watermelon recorded initial percentage protein contents of 4.37,4.39,and 10.89,respectively,whereas their corresponding non-sterile wastes recorded initial percentage protein contents of 3.76,4.00,and 10.16,respectively.The results further show that percentage increase in protein content of sterile wastes after 14 days of fermentation were pineapple,35.01%;sweet potato,27.60%;and watermelon,64.40%.Percentage increase in protein content of non-sterile wastes after 14 days of fermentation were pineapple,72.34%;sweet potato,85.25%;and watermelon,80.51%.These findings affirm the biotechnological potential of pineapple,sweet potato and watermelon wastes and the importance of fungi as agents for protein enrichment of agro-industrial wastes.展开更多
Apple pomace,a by-product of the apple juice processing industry,is significant agro-based waste in the Union Territory of Jammu&Kashmir,India.A considerable amount of apple is processed for juice production,resul...Apple pomace,a by-product of the apple juice processing industry,is significant agro-based waste in the Union Territory of Jammu&Kashmir,India.A considerable amount of apple is processed for juice production,resulting in a surplus of apple pomace,which is generally underutilized.This study utilized apple pomace as a substrate matrix for producing pullulan by applying Aureobasidium pullulans MTCC 1991.Solid-state fermentation was done at a lab scale in Erlenmeyer flasks to produce this exopolysaccharide.The suitable conditions for solid-state fermentation of apple pomace were:a solid-liquid ratio of 1:4,inoculum volume of 3 mL,pH 6 and incubation time of 14 days.A yield of 42 mg/g dw was obtained in the control sample without adding any other nutrients or chemicals.The addition of yeast extract at a concentration of 1%(w/w)and sucrose(5%w/w)significantly(p<0.05)increased the yield to 62 mg/g dw and 75 mg/g dw,respectively.The purified pullulan had similar characteristics to that of standard pullulan,as confirmed by ATR-FTIR,H NMR and TLC techniques.Pullulan production from apple pomace can be a better way to utilize this by-product from the apple juice industry.It will be a cost-effective technique for pullulan production as an inexpensive substrate and an environmentally friendly approach are also used.展开更多
文摘This study is to assess the biotechnological potential of agro-industrial wastes of pineapple(Ananas comosus),sweet potato(Ipomoea batatas)and watermelon(Citrullus lanatus)for protein enrichment by solid-state fermentation using Aspergillus niger.Spore suspensions of A.niger were prepared with potato dextrose broth that had been supplemented with sodium chloride,ammonium nitrate and thiamine,and adjusted to pH of 5.Initial protein contents of sterile and non-sterile wastes of the pineapple,sweet potato,and watermelon were determined by the Kjeldahl method.The remaining sterile and non-sterile wastes was inoculated with spore suspensions of A.niger and incubated at temperature of 24℃ under conditions of solid-state fermentation for 14 days.Protein contents of the inoculated sterile and non-sterile wastes were determined after 7 and 14 days.The sterile wastes of pineapple,sweet potato and watermelon recorded initial percentage protein contents of 4.37,4.39,and 10.89,respectively,whereas their corresponding non-sterile wastes recorded initial percentage protein contents of 3.76,4.00,and 10.16,respectively.The results further show that percentage increase in protein content of sterile wastes after 14 days of fermentation were pineapple,35.01%;sweet potato,27.60%;and watermelon,64.40%.Percentage increase in protein content of non-sterile wastes after 14 days of fermentation were pineapple,72.34%;sweet potato,85.25%;and watermelon,80.51%.These findings affirm the biotechnological potential of pineapple,sweet potato and watermelon wastes and the importance of fungi as agents for protein enrichment of agro-industrial wastes.
文摘Apple pomace,a by-product of the apple juice processing industry,is significant agro-based waste in the Union Territory of Jammu&Kashmir,India.A considerable amount of apple is processed for juice production,resulting in a surplus of apple pomace,which is generally underutilized.This study utilized apple pomace as a substrate matrix for producing pullulan by applying Aureobasidium pullulans MTCC 1991.Solid-state fermentation was done at a lab scale in Erlenmeyer flasks to produce this exopolysaccharide.The suitable conditions for solid-state fermentation of apple pomace were:a solid-liquid ratio of 1:4,inoculum volume of 3 mL,pH 6 and incubation time of 14 days.A yield of 42 mg/g dw was obtained in the control sample without adding any other nutrients or chemicals.The addition of yeast extract at a concentration of 1%(w/w)and sucrose(5%w/w)significantly(p<0.05)increased the yield to 62 mg/g dw and 75 mg/g dw,respectively.The purified pullulan had similar characteristics to that of standard pullulan,as confirmed by ATR-FTIR,H NMR and TLC techniques.Pullulan production from apple pomace can be a better way to utilize this by-product from the apple juice industry.It will be a cost-effective technique for pullulan production as an inexpensive substrate and an environmentally friendly approach are also used.
