In searching for new sources of oil, protein and gelatin researchers have investigated many wild plants, but our research group took a different approach: We looked at insects as oil, protein and gelatin source for bo...In searching for new sources of oil, protein and gelatin researchers have investigated many wild plants, but our research group took a different approach: We looked at insects as oil, protein and gelatin source for both nutritional and industrial applications. According to Sudanese indigenous knowledge, many insects have food and medicinal uses. We targeted two of these insects for our research:Aspongopus vidiuatus(melon bug) and Agonoscelis pubescens(sorghum bug). The two insects showed 27.0% and 28.2% crude protein, 45% and 60% oil, respectively. The oils contained 46.5% and 40.9% oleic acid, 3.4% and 34.5% linoleic acid, 44.2% and 12.1% palmitic acid and traces of linolenic acid,respectively. The tocopherol content of theseoils amounted to 0.3 and 34.0 mg/100g oil, respectively. The total content of sterols in the two oils was 17 and 450 mg/100g oil, respectively, whereasβ-sitosterol was determined as the main compound in all oils with about 60% of the totalsterol. The oxidative stability of the oils, asmeasured by the Rancimat test at 120°C, was 38 and 5.1 h, respectively. Edible gelatin was extracted from the two insect using hot water and mild acid and distilled water. SDS-PAGE patterns ofthe insect gelatins had very low molecularweight chains, and the two gelatins contained 40 kDa asmain component, differential scanning calorimetry results confirmed the difference betweenextraction methods concerning the extracted gelatin quality. FTIR spectra of melon and sorghum bug gelatins were similar and the absorption bands were situated in more than 6 bands in melon bug gelatin and only 6 bands in sorghum bug gelatin. Microstructures of the insect gelatinexamined with the scanning electron microscope showed that melon bug exhibited the finest gelatin network with very small voids. Melon bug gelatin showed the finer structure with smallerprotein strands and voids than sorghum buggelatin. Ice cream was made by using 0.5% insect’sgelatine and compared with that made using 0.5%commercial gelatine as stabilizing agent. The properties of the obtained ice cream produced using insects gelatine were found to be acceptable for the panelists, and no significant differences between ice cream made using insect gelatine when compared with that made using commercial gelatine in their general preferences The behavior of the crude Sorghum bug oil during deep-frying of par-fried potatoes was studied with regard to chemical, physical, and sensory parameters, such as the content ofFFA, tocopherols, polar compounds, oligomerTG, volatile compounds, oxidative stability, and totaloxidation (TOTOX) value. The results showed that the oil was suitable for deep-frying of potatoes. The oxidative stability of sunflower kernel oil was improved by blending with melon bug oil, the oxidative stability in the Rancimat test was improved from 5% to 68% compared to the control, with increasing parts of MBO, respectively. The insect oils were transesterified using methanol or ethanol in the presence of sulfuricacid to obtain biodiesel. The obtained insectbiodiesel characteristics were studied in accordance with the DIN EN 14214 specifications for biodiesel. It was possible to prepare the methyl and ethyl esters catalyzed by H2SO4 from the two insect oils.展开更多
Gelatins extracted from two edible insects Aspongubus viduatus （melon bug） and Agonoscelis pubescens （sorghum bug） were studied. The two insects showed 27.0 and 28.2% crude protein, respectively. Extraction of gel...Gelatins extracted from two edible insects Aspongubus viduatus （melon bug） and Agonoscelis pubescens （sorghum bug） were studied. The two insects showed 27.0 and 28.2% crude protein, respectively. Extraction of gelatin using hot water gave high yield followed by mild acid and distilled water extraction, respectively. SDS-PAGE pattern showed low molecular weight chains, and the two gelatins contained protein with molecular weight of 40 kDa as main component. The differential scanning calorimetry thermograms results confirm no difference between extraction methods concerning the extracted gelatin quality. FTIR spectra of melon and sorghum bug gelatins were similar and the absorption bands were situated in more than 6 bands in melon bug gelatin and only 6 bands in sorghum bug gelatin. Amide II bands of gelatins from both melon and sorghum bug appeared at around 1554 cm^-1, while Amide I bands （1734-1632 cmt） appeared only in melon bug method 2 （MB2） and melon bug method3 （MB3）. Microstructures of the insect gelatin examined with the scanning electron microscope showed that melon bug exhibited the finest gelatin network with very small voids. Melon bug gelatin showed finer structure with smaller protein strands and voids than sorghum bug gelatin.展开更多
文摘In searching for new sources of oil, protein and gelatin researchers have investigated many wild plants, but our research group took a different approach: We looked at insects as oil, protein and gelatin source for both nutritional and industrial applications. According to Sudanese indigenous knowledge, many insects have food and medicinal uses. We targeted two of these insects for our research:Aspongopus vidiuatus(melon bug) and Agonoscelis pubescens(sorghum bug). The two insects showed 27.0% and 28.2% crude protein, 45% and 60% oil, respectively. The oils contained 46.5% and 40.9% oleic acid, 3.4% and 34.5% linoleic acid, 44.2% and 12.1% palmitic acid and traces of linolenic acid,respectively. The tocopherol content of theseoils amounted to 0.3 and 34.0 mg/100g oil, respectively. The total content of sterols in the two oils was 17 and 450 mg/100g oil, respectively, whereasβ-sitosterol was determined as the main compound in all oils with about 60% of the totalsterol. The oxidative stability of the oils, asmeasured by the Rancimat test at 120°C, was 38 and 5.1 h, respectively. Edible gelatin was extracted from the two insect using hot water and mild acid and distilled water. SDS-PAGE patterns ofthe insect gelatins had very low molecularweight chains, and the two gelatins contained 40 kDa asmain component, differential scanning calorimetry results confirmed the difference betweenextraction methods concerning the extracted gelatin quality. FTIR spectra of melon and sorghum bug gelatins were similar and the absorption bands were situated in more than 6 bands in melon bug gelatin and only 6 bands in sorghum bug gelatin. Microstructures of the insect gelatinexamined with the scanning electron microscope showed that melon bug exhibited the finest gelatin network with very small voids. Melon bug gelatin showed the finer structure with smallerprotein strands and voids than sorghum buggelatin. Ice cream was made by using 0.5% insect’sgelatine and compared with that made using 0.5%commercial gelatine as stabilizing agent. The properties of the obtained ice cream produced using insects gelatine were found to be acceptable for the panelists, and no significant differences between ice cream made using insect gelatine when compared with that made using commercial gelatine in their general preferences The behavior of the crude Sorghum bug oil during deep-frying of par-fried potatoes was studied with regard to chemical, physical, and sensory parameters, such as the content ofFFA, tocopherols, polar compounds, oligomerTG, volatile compounds, oxidative stability, and totaloxidation (TOTOX) value. The results showed that the oil was suitable for deep-frying of potatoes. The oxidative stability of sunflower kernel oil was improved by blending with melon bug oil, the oxidative stability in the Rancimat test was improved from 5% to 68% compared to the control, with increasing parts of MBO, respectively. The insect oils were transesterified using methanol or ethanol in the presence of sulfuricacid to obtain biodiesel. The obtained insectbiodiesel characteristics were studied in accordance with the DIN EN 14214 specifications for biodiesel. It was possible to prepare the methyl and ethyl esters catalyzed by H2SO4 from the two insect oils.
文摘Gelatins extracted from two edible insects Aspongubus viduatus （melon bug） and Agonoscelis pubescens （sorghum bug） were studied. The two insects showed 27.0 and 28.2% crude protein, respectively. Extraction of gelatin using hot water gave high yield followed by mild acid and distilled water extraction, respectively. SDS-PAGE pattern showed low molecular weight chains, and the two gelatins contained protein with molecular weight of 40 kDa as main component. The differential scanning calorimetry thermograms results confirm no difference between extraction methods concerning the extracted gelatin quality. FTIR spectra of melon and sorghum bug gelatins were similar and the absorption bands were situated in more than 6 bands in melon bug gelatin and only 6 bands in sorghum bug gelatin. Amide II bands of gelatins from both melon and sorghum bug appeared at around 1554 cm^-1, while Amide I bands （1734-1632 cmt） appeared only in melon bug method 2 （MB2） and melon bug method3 （MB3）. Microstructures of the insect gelatin examined with the scanning electron microscope showed that melon bug exhibited the finest gelatin network with very small voids. Melon bug gelatin showed finer structure with smaller protein strands and voids than sorghum bug gelatin.
