Agave-derived products,such as syrup and fructans,contain functional compounds with bioactive properties.The antimicrobial activity of hydrolyzed agave syrup(HAS),partially hydrolyzed agave syrup(PHAS),and agave fruct...Agave-derived products,such as syrup and fructans,contain functional compounds with bioactive properties.The antimicrobial activity of hydrolyzed agave syrup(HAS),partially hydrolyzed agave syrup(PHAS),and agave fructans(AF)against Salmonella enterica subsp.enterica serovar Typhimurium derived from ATCC®14028^(TM*),Escherichia coli derived from CDC AR-0346,and Listeria monocytogenes derived from ATCC®19111^(TM*)was evaluated.The growth kinetics of S.Typhimurium,E.coli,and L.monocytogenes with or without treatments were used to calculate the maximum specific growth rate(μ_(max)),lag time(λ),stationary phase(Y_(max)),and correlation coefficient(R^(2)).The antibacterial activity of HAS,PHAS,and AF against S.Typhimurium,E.coli,and L.monocytogenes was evaluated using the well diffusion method,and biofilm formation was assessed using the crystal violet method.All assays were conducted in triplicate.ANOVA and Tukey’s post hoc test were employed to evaluate differences between treatments,with p<0.05 considered significant.HAS,PHAS,and AF inhibit the growth of S.Typhimurium,E.coli,and L.monocytogenes in a dose-dependent manner.The microbial growth model shows that HAS,PHAS,and AF decreaseμ_(max),increaseλ,and decrease Y_(max)in the three evaluated strains.Moreover,HAS and PHAS treatments show a larger zone of inhibition than AF.Likewise,the addition of HAS,PHAS,and AF inhibits biofilm formation.Based on the above,this study shows that agave-derived products possess antibacterial and antibiofilm properties,which can replace the use of antibiotics in the food industry.展开更多
基金Secretariat of Science,Humanities,Technology,and Innovation(SECIHTI)in Mexico for providing funding to Azucena Rodríguez-Lozano for her Master’s degree studies in the Interinstitutional Master’s Degree in Livestock Production at the Uni-versity of Guanajuato,Mexico.
文摘Agave-derived products,such as syrup and fructans,contain functional compounds with bioactive properties.The antimicrobial activity of hydrolyzed agave syrup(HAS),partially hydrolyzed agave syrup(PHAS),and agave fructans(AF)against Salmonella enterica subsp.enterica serovar Typhimurium derived from ATCC®14028^(TM*),Escherichia coli derived from CDC AR-0346,and Listeria monocytogenes derived from ATCC®19111^(TM*)was evaluated.The growth kinetics of S.Typhimurium,E.coli,and L.monocytogenes with or without treatments were used to calculate the maximum specific growth rate(μ_(max)),lag time(λ),stationary phase(Y_(max)),and correlation coefficient(R^(2)).The antibacterial activity of HAS,PHAS,and AF against S.Typhimurium,E.coli,and L.monocytogenes was evaluated using the well diffusion method,and biofilm formation was assessed using the crystal violet method.All assays were conducted in triplicate.ANOVA and Tukey’s post hoc test were employed to evaluate differences between treatments,with p<0.05 considered significant.HAS,PHAS,and AF inhibit the growth of S.Typhimurium,E.coli,and L.monocytogenes in a dose-dependent manner.The microbial growth model shows that HAS,PHAS,and AF decreaseμ_(max),increaseλ,and decrease Y_(max)in the three evaluated strains.Moreover,HAS and PHAS treatments show a larger zone of inhibition than AF.Likewise,the addition of HAS,PHAS,and AF inhibits biofilm formation.Based on the above,this study shows that agave-derived products possess antibacterial and antibiofilm properties,which can replace the use of antibiotics in the food industry.
