Two new spirostanol sapogenins named agavegenin A and B were isolated from the fermented leaves of Agave americana L. Their structures were elucidated as (23S, 25R)-5a- spirostan-3b, 6a, 11a, 23-tetraol (1) and (23S,...Two new spirostanol sapogenins named agavegenin A and B were isolated from the fermented leaves of Agave americana L. Their structures were elucidated as (23S, 25R)-5a- spirostan-3b, 6a, 11a, 23-tetraol (1) and (23S, 25S)-5a-spirostan-3b, 23, 27-triol (2) by spectral methods.展开更多
Agave americana Var. Marginata Hort. （Agavaceae） is a perennial herb. This paper summarized its classification, distribution, morphological characteristics, habit, flowering and fruiting, cultivation and reproductio...Agave americana Var. Marginata Hort. （Agavaceae） is a perennial herb. This paper summarized its classification, distribution, morphological characteristics, habit, flowering and fruiting, cultivation and reproduction, as well as multiple uses in daily life, which provided references for the exploitation and utilization of Agave americana Vat. Marginata Hort. （Agavaceae）.展开更多
The reduction behavior and metallization degree of magnetite concentrate with agave bagasse were investigated in an inert atmosphere.The effects of temperature,biomass content,and residence time on reduction experimen...The reduction behavior and metallization degree of magnetite concentrate with agave bagasse were investigated in an inert atmosphere.The effects of temperature,biomass content,and residence time on reduction experiments and metallization degree were investigated by X-ray diffraction and scanning electron microscopy.Compared with other types of biomass,agave bagasse had lower contents of nitrogen,sulfur,and ash.X-ray diffraction analysis showed that the metallization degree improved with increasing temperature and biomass content.Complete metallization was achieved at 1100℃ for 30 min with 65:35 and 50:50 ratios of the magnetite concentrate to the agave bagasse.These results demonstrate that agave bagasse promotes the efficient metallization of magnetite concentrate without the external addition of a reducing agent.Therefore,this biomass is a technical suitable alternative to replace fossil fuels in steelmaking.展开更多
Two new spirostanol glycosides named agamenoside A and B, were isolated from the fermented leaves of Agave americana. Their structures were elucidated as (23S, 25R)-5a -spirostan-3b, 6a, 23-triol 3-O-a-L-rhamnopyrano...Two new spirostanol glycosides named agamenoside A and B, were isolated from the fermented leaves of Agave americana. Their structures were elucidated as (23S, 25R)-5a -spirostan-3b, 6a, 23-triol 3-O-a-L-rhamnopyranosyl-(13)- b-D-glucopyranosyl-(12)-[b-D- xylopyranosyl-(13)]-b-D-glucopyranosyl-(14)-b-D-galactopyranoside (1) and (25R)-5a-spiro- stan-3b, 6a-diol 3-O-b-D-glucopyranosyl-(12)-[ b-D-xylopyranosyl-(13)]- b-D-glucopyra- nosyl-(14)- b-D-galactopyranoside (2) by a combination of chemical and spectral methods.展开更多
The current work is focused on the study of the bio-sorption of hexavalent chromium from aqueous solution using sisal natural fiber(Agave sisalana) treated by various chelating agents(ligands) such as urea(UR),thiocar...The current work is focused on the study of the bio-sorption of hexavalent chromium from aqueous solution using sisal natural fiber(Agave sisalana) treated by various chelating agents(ligands) such as urea(UR),thiocarbamide(TC), ethylenediaminetetraacetic acid(EDTA), and diphenyl carbazide(DCZ). The fiber treatments were investigated using Fourier Transform Infrared Spectroscopy(FTIR) and Scanning electron microscope(SEM). The kinetics of chromium bio-sorption was studied in batch presses under the effect of some physicochemical factors such as the nature of chelating agent(F@UR, F@TC, F@DCZ, and F@EDTA),adsorbent dose(2–10 g·L^(-1)), chromium initial concentration(100–500 mg·L^(-1)), solution pH(1–6), and batch temperature(20 ℃–50 ℃). This study resulted in an optimum adsorption at a chromium initial concentration of 100 mg·L^(-1), at pH 2, and at 20 ℃. The obtained results showed clearly that the treatment with chelating agent boosts the adsorptive capacity of A. sisalana fibers Cr(VI) 10.9 mg·g^(-1) to 58.6 mg·g^(-1). The modeling study showed that the adsorption kinetics obey the pseudo-second-order model, with an R2 in the range of 0.991 and 0.999. The bio-sorption isotherms followed the Langmuir model; the maximum uptake capacity of(F@N, F@UR,F@TC, F@DCZ, and F@EDTA) was found to be respectively, 12.3 mg·g^(-1), 25.33 mg·g^(-1), 28.73 mg·g^(-1),42.54 mg·g^(-1), and 61.45 mg·g^(-1). The determined adsorption thermodynamics parameters such as enthalpy,free energy, and entropy showed that the adsorption process is exothermic, spontaneous, and has a stable configuration.展开更多
A review is presented for give information and highlights the advantage of ISTR as molecular marker which with the genome changes after manipulation or breeding intervention can be detected.
A new minor pregnane glycoside was isolated from the fermented leaves of Agave americana. Its structure was elucidated as (20S)-5a-pregnane-3? 20-diol 20-O--D-glucopyrano- side (1) by spectral methods.
In this study,the fibers of invasive species Agave americana L.and Ricinus communis L.were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.Cell...In this study,the fibers of invasive species Agave americana L.and Ricinus communis L.were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.Cellulose nanofibers were obtained by two methods,based on either alkaline or acid hydrolysis.The morphology,chemical composition,and crystallinity of the obtained materials were characterized by scanning electron microscopy(SEM)together with energy-dispersive X-ray spectroscopy(EDX),dynamic light scattering(DLS),X-ray diffraction(XRD),and Fourier transform infrared(FTIR)spectroscopy.The crystallinity indexes(CIs)of the cellulose nanofibers extracted from A.americana and R.communis were very high(94.1%and 92.7%,respectively).Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293 T(HEK293 T)cells were also performed.The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed(i.e.,0-500μg/mL).Our results showed that the nanocellulose extracted from A.americana and R.communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.展开更多
Agave salmiana ash and poultry eggshell powder as CaO sources were used for obtaining nanostructured hydro-xyapatite(HAP).The synthesis was carried out by the Green Chemistry Hydrothermal Biosynthesis at 180℃ with a ...Agave salmiana ash and poultry eggshell powder as CaO sources were used for obtaining nanostructured hydro-xyapatite(HAP).The synthesis was carried out by the Green Chemistry Hydrothermal Biosynthesis at 180℃ with a pH of 5,by reacting CaO from Agave Salmiana ash and Eggshell powder,with dibasic caldium phosphate(CaH-PO_(4)+2H_(2)O)in an aqueous solution,with Aloe barbadensis extract.The product was characterized by X-ray dif-fraction,Fourier transform infrared spectroscopy(FT-IR),Scanning Electron Microscopy(SEM),and Transmission Electron Microscopy(TEM).The size and shape of the hydroxyapatite particles changed dramati-cally in the presence of Aloe barbadensis.Large crystals of Hydroxyapatite were observed when Eggshell powder and Agave salmiana ash were used as raw materials in the presence of the Aloe barbadensis surfactant.Crystals with shapes of ribbons and plates from 1 micrometers to 8 micrometers were observed when using the eggshell powder in the presence of Aloe bar badensis and,in the case of Agave salmiana ash in the presence of Aloe bar-badensis,crystals with shapes of quadra ngular prisms and hexagonal(polyhedra)with sizes from 2 micrometers to 20 micrometers were observed.Hydroxyapatite was therefore successfully biosynthesized by a green and sus-tainable method that reduces the environmental impact.展开更多
Agave durangensis propagates basically by seeds. This species is economically important because it supports a mescal industry in Durango, Mexico. At present, its exploitation is overall by collecting plants from the w...Agave durangensis propagates basically by seeds. This species is economically important because it supports a mescal industry in Durango, Mexico. At present, its exploitation is overall by collecting plants from the wild populations. In order to reduce the negative impact of the collection in the natural populations of A. durangensis and to improve the mescal industry, it is necessary to establish plantations with selected seeds for a high vigor. In this paper, the variation in morphological features, physiological behavior of germination, and biochemical indicators of seed vigor among three natural populations of A. durangensis was assessed. Variation was found in the seed weight (0.68 to 1.15 mg/seed), seed dimensions (3.51 × 5.29 to 4.62 × 5.92 mm), germinability reduction at 15°°C related to 25°C (4% to 51%), germination rate at 25°C (44.0 to 48.5 seeds/day) and at 15°C (5.17 to 6.77 seeds/day), development reduction at 15°C related to 25°C (86.66% to 91.99%), levels of seed accumulated phenols (71 to 85 μg/seed), antioxidant potential (42% to 50% reduction of DPPH*), seed alcohol dehydrogenase activity (ADH) (180 to 1100 μmol NAD+/mg protein/min), highest ADH activity after imbibitions at 25°C (310.24 to 520.2 μmol NAD+/mg protein/min), and highest ADH activity after imbibitions at 15°C (170.74 to 440.71 μmol NAD+/mg protein/min). The variation in the seed vigor was revealed by a principal component analysis (PCA) based on all the parameters evaluated. PCA clearly discriminated among the three populations.展开更多
The aim of the study was to contribute to the information on agave soluble fibers since research has been focused on chicory fiber but not in agave products;thus we assess the acute toxicity and genotoxicity of two or...The aim of the study was to contribute to the information on agave soluble fibers since research has been focused on chicory fiber but not in agave products;thus we assess the acute toxicity and genotoxicity of two organic and high purity dietary soluble fibers from agave, Metlin? and Metlos?. We performed an acute toxicity assay in Hsd:ICR mice and Hsd:Wistar rats and an in vivo genotoxic test. Results showed that there are no deaths at any doses or genotoxicity, so it can be concluded that these products are non-toxic, at the administrated doses and none showed a cytotoxic, clastogenic or aneuploidic effect.展开更多
A review is presented about the studies that have been carried out on the phenolic composition of the species of the genus Agave in order to show which species of the genus has been analyzed, what is the potential of ...A review is presented about the studies that have been carried out on the phenolic composition of the species of the genus Agave in order to show which species of the genus has been analyzed, what is the potential of the species of Agave as source of phenolics with important biological activities, and what is the significance of the phenolic profiles as chemotaxonomic markers in the genus. The results of the analysis of a dozen of Agave species reveal an important richness of phenolic compounds, including flavonoids, homoisoflavonoids, and phenolic acids, and a broad diversity of biological activities associated to those compounds, as antioxidant, antibacterial, antifungal, antinematod, and immunomodulatory capabilities. The phenols of Agave also have a major potential as specific chemotaxonomic markers.展开更多
Adverse weather conditions and intensive use for fiber production have caused decline of natural populations of Agave lechuguilla. By these reasons, it is important to develop a sustainable management of this specie t...Adverse weather conditions and intensive use for fiber production have caused decline of natural populations of Agave lechuguilla. By these reasons, it is important to develop a sustainable management of this specie to avoid this situation. One of the first steps is to establish forest plantations as an alternative to restore A. lechuguilla habitat and increase its range while at the same time indirectly reducing erosion rates. The aim of this study was to identify the potential areas for establishment of plantations of A. lechuguilla in the State of Coahuila, Mexico. Areas for planting establishment were selected according to multi-criteria analysis using Geographic Information System (GIS) Idrisi32 Ver 2.0 and 3.2 Arc.View. The analysis was based on comparison of climate and soil requirements in contrast to environmental conditions of the tested area. The evaluated variables were: soil type, texture, depth, average annual temperature, annual precipitation, elevation and slope. Results derive in potential area maps, geo-referenced and their distribution by Agricultural Development District. An area of 5 million 159,273 hectares was determined with potential for establishment of A. lechuguilla forest plantations in the State of Coahuila. It was concluded that, use of GIS is a reliable tool to determine potential areas for A. lechuguilla forest plantations, where this specie has higher probability of success in survival and biomass production. This study will support programs aimed to strengthen the economy of rural producers and also recover and preserve the arid state.展开更多
Here, we evaluated the possibility of growing grape tomatoes on three different mixtures of composted agave bagasse substrate. Tomatoes were grown in 28-L pots for 106 days under a drip irrigation system with a hydrop...Here, we evaluated the possibility of growing grape tomatoes on three different mixtures of composted agave bagasse substrate. Tomatoes were grown in 28-L pots for 106 days under a drip irrigation system with a hydroponic crop nutrient solution. The average production of grape tomatoes was 338.9, 358.9, and 325.7 g/pot/cut for each of the substrates studied, and no significant difference was observed between treatments (p > 0.5). The mean of numbers of grape tomatoes were 34.6, 38.8, and 34.2/pot/cut for each of the substrates studied, and no significant difference was observed between treatments (p > 0.5). The mean weight of individual grape tomatoes was about 10.4 g for all of the substrates tested. These results confirmed the notion that a composted agave bagasse substrate could replace an expensive imported substrate. The remaining challenge is to produce agave bagasse substrate commercially, because to date, agave bagasse substrates have only been produced on a pilot scale for testing. Therefore, it is necessary to implement a stabilized process for producing agave bagasse at a commercial level. This process must consider economic production costs and market value to ensure that the product is competitive with other substrates typically used in soilless crops.展开更多
Kel l ey Lee是一位年纪轻轻的主厨,她打造了上海几家最为知名、最受欢迎的餐厅（拳击猫Boxi n g Cat Brewery、龙舌兰小馆Cantina Agave和豆苗工坊Sproutworks）Kelley是一位极为要强的女商人,她在上海的10年职业生涯中开设了19家餐厅...Kel l ey Lee是一位年纪轻轻的主厨,她打造了上海几家最为知名、最受欢迎的餐厅（拳击猫Boxi n g Cat Brewery、龙舌兰小馆Cantina Agave和豆苗工坊Sproutworks）Kelley是一位极为要强的女商人,她在上海的10年职业生涯中开设了19家餐厅。她的最新餐饮概念一Liquid Laundry是一家具有纽约风味的美食酒吧,占地900平方米,现已成为在上海度过悠闲时光的热门场地。展开更多
The blue agave (Agave tequilana Weber variety azul) is a species that is widely used to produce tequila. For this reason, in the last decade, large extensions of terrain have been established for its cultivation. Howe...The blue agave (Agave tequilana Weber variety azul) is a species that is widely used to produce tequila. For this reason, in the last decade, large extensions of terrain have been established for its cultivation. However, much of this land has been abandoned in a short time because at the beginning it was not known that at least six years were needed before the agave could be harvested. However, when the growers wanted to reintegrate some of these crops into the productive process, their exact age and the cultural activities needed for harvesting the agave were unknown because information regarding its vegetative development relative to its age was lacking. The hypothesis of this work was: If the morphological properties reflect the development state of the population individuals, then the properties height, basal area, rosette diameter, the north, south, east and west leaf, leaves number and foliar verticil will help to characterize the age categories in Agave tequilana Weber variety azul population under cultivated conditions. To this end, six plots with plants of different ages (one to six years) were selected in the municipality of Llera, Tamaulipas, Mexico. In each plot, 60 individuals were selected at random, and their morphological properties (height, basal area, rosette diameter, northern leaf, southern leaf, eastern leaf, western leaf;number of leaves and leaf whorl) were measured. With these data, a principal component analysis was performed to determine which of these properties explains the most variation of the data. The results indicate that height and basal area are the properties that are most closely associated with plant age. It is also for the first time demonstrated that harvest time can be reduced by one or two years, which favors producers economically because it could generate savings in production costs.展开更多
Somatic embryogenesis is a very efficient way to propagate economically important plants;however, not all genotypes within a species can be propagated using this method, as a combined effect of both genetic and epigen...Somatic embryogenesis is a very efficient way to propagate economically important plants;however, not all genotypes within a species can be propagated using this method, as a combined effect of both genetic and epigenetic mechanisms may be involved in the response. The aim of the present study was to perform a comparative analysis of the genetic differences through amplified fragment length polymorphism (AFLP) and the epigenetic differences through methylation-sensitive amplified polymorphism (MSAP) of two Agave fourcroydes clonal lines, one highly embryogenic (K33) and the other non-embryogenic (K7). Genetic and epigenetic variabilities existed within each clonal line;however, the polymorphic profiles from the two marker systems allowed us to clearly distinguish the two clonal lines before somatic embryogenesis induction. During the induction, the changes detected were mainly 1) unmethylated fragments in the initial explants that were methylated during induction (methylation events) and 2) fragments with different methylation states in the initial explant that were unmethylated in some stages of the process (demethylation events). K33 showed greater dynamism in relation to methylation/demethylation events, while K7 presented the methylation events in a more constant range and at higher levels during all process.展开更多
This paper presents an overview of different handling systems and use of the agave bagasse. These systems have appeared from different research works always taking in account the environmental sustainability. It is me...This paper presents an overview of different handling systems and use of the agave bagasse. These systems have appeared from different research works always taking in account the environmental sustainability. It is mentioned that the agave bagasse can be used for animal feeding, for the elaboration of compound materials, as an element for agricultural and hydroponic vegetables cultivation purposes, and also as a means to treat biosolids, vinasses, and bagasses of slaughterhouses and tanneries.展开更多
The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana L.In order to do the establishment of cells,the formation of callus was induced;for it...The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana L.In order to do the establishment of cells,the formation of callus was induced;for it two auxins were evaluated:2,4-dichlorophenoxyacetic acid(2,4-D)and 4-mino-3,5,6-trichloropicolinic acid(picloram)at three concentrations(0.25,0.5 and 0.75 mg L−1)in three explants(leaf,root and meristems)cultured in MS semisolid medium.The callogenesis response was related to the type and section of the explant,as well as the regulator used,and a cell suspension was established using 0.5 mg L−1 naphthaleneacetic acid(NAA)+0.5 mg L−1 Benzylaminopurine(BAP).Seedlings were exposed to polyethyleneglycol(15%and 30%w/v)with a water potential of−0.87 and−2.67 MPa,respectively,under soil conditions.Water stress was applied through restricted irrigation.Fresh weight,root system growth,and chlorophyll concentration were some of the parameters that were affected by the effect of water deficit on A.americana L.Chlorophyll concentration values were significantly decreased by 15 at 30%PEG(19.6 SPAD units)compared to the control treatment.In in vitro plants,the highest concentration of proline was found in the roots,being the treatment with 30%polyethylene glycol where the highest concentration of this osmoregulator was obtained(62.5 mg g−1 DW).Under restricted irrigation conditions,an increase in proline concentration was observed both in the aerial part(2.2μg 100 g−1 DW)and in the root system(1.8μg 100 g−1 DW).However,the concentrations found were approximately ten times greater,less than those found under in vitro conditions.Therefore,the accumulation of proline can be considered an indicator of stress in Agave Americana L.growth in vitro.展开更多
The Agave genus has played a key role in the food security of communities at the local and regional level for millennia.The use and knowledge of these plants led to the development of the oldest agricultural centres i...The Agave genus has played a key role in the food security of communities at the local and regional level for millennia.The use and knowledge of these plants led to the development of the oldest agricultural centres in America.Therefore,a review of the literature on the ethnic and cultural particularities of the use of agave species in food and current knowledge on their nutritional contribution to the human diet is presented.56 species of agaves with food uses were registered by 55 ethnic groups in the American continent.Of this total,23 species also have a medicinal purpose.The number of agave species used by these ethnic groups shows an important agromorphological diversity.People collect and cultivate agaves to use their stem and foliar bases,the floral scape,the flowers and flower buds,the leaves,and the sap to prepare different foods.In many cases these foods help them survive in adverse conditions.The contribution of agaves to human nutrition has been studied from nutraceutical components such as saponins,inulin,fructans,phenolic compounds and antioxidants,dietary fiber,amino acids,and minerals.In the face of global climate change,agaves represent a strategic biological resource for the world,making their use,conservation and study a priority.Agave products will continue to show their health benefits.Human use of agaves aids diversification of the genus while contributing to the well-being and permanence of a variety of cultures.展开更多
文摘Two new spirostanol sapogenins named agavegenin A and B were isolated from the fermented leaves of Agave americana L. Their structures were elucidated as (23S, 25R)-5a- spirostan-3b, 6a, 11a, 23-tetraol (1) and (23S, 25S)-5a-spirostan-3b, 23, 27-triol (2) by spectral methods.
文摘Agave americana Var. Marginata Hort. （Agavaceae） is a perennial herb. This paper summarized its classification, distribution, morphological characteristics, habit, flowering and fruiting, cultivation and reproduction, as well as multiple uses in daily life, which provided references for the exploitation and utilization of Agave americana Vat. Marginata Hort. （Agavaceae）.
基金National Science and Technology Council(CONACYT)for the grant No.665811 awarded to his Master thesis。
文摘The reduction behavior and metallization degree of magnetite concentrate with agave bagasse were investigated in an inert atmosphere.The effects of temperature,biomass content,and residence time on reduction experiments and metallization degree were investigated by X-ray diffraction and scanning electron microscopy.Compared with other types of biomass,agave bagasse had lower contents of nitrogen,sulfur,and ash.X-ray diffraction analysis showed that the metallization degree improved with increasing temperature and biomass content.Complete metallization was achieved at 1100℃ for 30 min with 65:35 and 50:50 ratios of the magnetite concentrate to the agave bagasse.These results demonstrate that agave bagasse promotes the efficient metallization of magnetite concentrate without the external addition of a reducing agent.Therefore,this biomass is a technical suitable alternative to replace fossil fuels in steelmaking.
基金This work was financially supported by the National Natural Science Foundation of China (NSFC) (39969005)The authors wish to thanks the members of analytic group of Laboratory of Phytochemistry,Kunming Institute of Botany for spectral measurements.
文摘Two new spirostanol glycosides named agamenoside A and B, were isolated from the fermented leaves of Agave americana. Their structures were elucidated as (23S, 25R)-5a -spirostan-3b, 6a, 23-triol 3-O-a-L-rhamnopyranosyl-(13)- b-D-glucopyranosyl-(12)-[b-D- xylopyranosyl-(13)]-b-D-glucopyranosyl-(14)-b-D-galactopyranoside (1) and (25R)-5a-spiro- stan-3b, 6a-diol 3-O-b-D-glucopyranosyl-(12)-[ b-D-xylopyranosyl-(13)]- b-D-glucopyra- nosyl-(14)- b-D-galactopyranoside (2) by a combination of chemical and spectral methods.
文摘The current work is focused on the study of the bio-sorption of hexavalent chromium from aqueous solution using sisal natural fiber(Agave sisalana) treated by various chelating agents(ligands) such as urea(UR),thiocarbamide(TC), ethylenediaminetetraacetic acid(EDTA), and diphenyl carbazide(DCZ). The fiber treatments were investigated using Fourier Transform Infrared Spectroscopy(FTIR) and Scanning electron microscope(SEM). The kinetics of chromium bio-sorption was studied in batch presses under the effect of some physicochemical factors such as the nature of chelating agent(F@UR, F@TC, F@DCZ, and F@EDTA),adsorbent dose(2–10 g·L^(-1)), chromium initial concentration(100–500 mg·L^(-1)), solution pH(1–6), and batch temperature(20 ℃–50 ℃). This study resulted in an optimum adsorption at a chromium initial concentration of 100 mg·L^(-1), at pH 2, and at 20 ℃. The obtained results showed clearly that the treatment with chelating agent boosts the adsorptive capacity of A. sisalana fibers Cr(VI) 10.9 mg·g^(-1) to 58.6 mg·g^(-1). The modeling study showed that the adsorption kinetics obey the pseudo-second-order model, with an R2 in the range of 0.991 and 0.999. The bio-sorption isotherms followed the Langmuir model; the maximum uptake capacity of(F@N, F@UR,F@TC, F@DCZ, and F@EDTA) was found to be respectively, 12.3 mg·g^(-1), 25.33 mg·g^(-1), 28.73 mg·g^(-1),42.54 mg·g^(-1), and 61.45 mg·g^(-1). The determined adsorption thermodynamics parameters such as enthalpy,free energy, and entropy showed that the adsorption process is exothermic, spontaneous, and has a stable configuration.
文摘A review is presented for give information and highlights the advantage of ISTR as molecular marker which with the genome changes after manipulation or breeding intervention can be detected.
基金supported by the National Natulal Science Foundation of China(NSFC)(39969005).
文摘A new minor pregnane glycoside was isolated from the fermented leaves of Agave americana. Its structure was elucidated as (20S)-5a-pregnane-3? 20-diol 20-O--D-glucopyrano- side (1) by spectral methods.
基金the Programa de Cooperación Territorial INTERREG V-A MAC 2014-2020 and Inv2Mac Project(MAC/4.6d/229)the partial support of FCT-Funda??o para a Ciência e a Tecnologia(Base Fund UIDB/00674/2020)+1 种基金ARDITI-Agência Regional para o Desenvolvimento da Investiga??o Tecnologia e Inova??o supported the study through the project M1420-01-0145FEDER-000005-CQM+(Madeira 14-20 Program)the Postdoc Grant(M1420-09-5369-FSE-000001,002458/2015/132)for Carla S.ALVES is also acknowledged。
文摘In this study,the fibers of invasive species Agave americana L.and Ricinus communis L.were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.Cellulose nanofibers were obtained by two methods,based on either alkaline or acid hydrolysis.The morphology,chemical composition,and crystallinity of the obtained materials were characterized by scanning electron microscopy(SEM)together with energy-dispersive X-ray spectroscopy(EDX),dynamic light scattering(DLS),X-ray diffraction(XRD),and Fourier transform infrared(FTIR)spectroscopy.The crystallinity indexes(CIs)of the cellulose nanofibers extracted from A.americana and R.communis were very high(94.1%and 92.7%,respectively).Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293 T(HEK293 T)cells were also performed.The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed(i.e.,0-500μg/mL).Our results showed that the nanocellulose extracted from A.americana and R.communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.
基金The funding was provided by Project CE1645-21 PAICYTUANL.
文摘Agave salmiana ash and poultry eggshell powder as CaO sources were used for obtaining nanostructured hydro-xyapatite(HAP).The synthesis was carried out by the Green Chemistry Hydrothermal Biosynthesis at 180℃ with a pH of 5,by reacting CaO from Agave Salmiana ash and Eggshell powder,with dibasic caldium phosphate(CaH-PO_(4)+2H_(2)O)in an aqueous solution,with Aloe barbadensis extract.The product was characterized by X-ray dif-fraction,Fourier transform infrared spectroscopy(FT-IR),Scanning Electron Microscopy(SEM),and Transmission Electron Microscopy(TEM).The size and shape of the hydroxyapatite particles changed dramati-cally in the presence of Aloe barbadensis.Large crystals of Hydroxyapatite were observed when Eggshell powder and Agave salmiana ash were used as raw materials in the presence of the Aloe barbadensis surfactant.Crystals with shapes of ribbons and plates from 1 micrometers to 8 micrometers were observed when using the eggshell powder in the presence of Aloe bar badensis and,in the case of Agave salmiana ash in the presence of Aloe bar-badensis,crystals with shapes of quadra ngular prisms and hexagonal(polyhedra)with sizes from 2 micrometers to 20 micrometers were observed.Hydroxyapatite was therefore successfully biosynthesized by a green and sus-tainable method that reduces the environmental impact.
文摘Agave durangensis propagates basically by seeds. This species is economically important because it supports a mescal industry in Durango, Mexico. At present, its exploitation is overall by collecting plants from the wild populations. In order to reduce the negative impact of the collection in the natural populations of A. durangensis and to improve the mescal industry, it is necessary to establish plantations with selected seeds for a high vigor. In this paper, the variation in morphological features, physiological behavior of germination, and biochemical indicators of seed vigor among three natural populations of A. durangensis was assessed. Variation was found in the seed weight (0.68 to 1.15 mg/seed), seed dimensions (3.51 × 5.29 to 4.62 × 5.92 mm), germinability reduction at 15°°C related to 25°C (4% to 51%), germination rate at 25°C (44.0 to 48.5 seeds/day) and at 15°C (5.17 to 6.77 seeds/day), development reduction at 15°C related to 25°C (86.66% to 91.99%), levels of seed accumulated phenols (71 to 85 μg/seed), antioxidant potential (42% to 50% reduction of DPPH*), seed alcohol dehydrogenase activity (ADH) (180 to 1100 μmol NAD+/mg protein/min), highest ADH activity after imbibitions at 25°C (310.24 to 520.2 μmol NAD+/mg protein/min), and highest ADH activity after imbibitions at 15°C (170.74 to 440.71 μmol NAD+/mg protein/min). The variation in the seed vigor was revealed by a principal component analysis (PCA) based on all the parameters evaluated. PCA clearly discriminated among the three populations.
文摘The aim of the study was to contribute to the information on agave soluble fibers since research has been focused on chicory fiber but not in agave products;thus we assess the acute toxicity and genotoxicity of two organic and high purity dietary soluble fibers from agave, Metlin? and Metlos?. We performed an acute toxicity assay in Hsd:ICR mice and Hsd:Wistar rats and an in vivo genotoxic test. Results showed that there are no deaths at any doses or genotoxicity, so it can be concluded that these products are non-toxic, at the administrated doses and none showed a cytotoxic, clastogenic or aneuploidic effect.
文摘A review is presented about the studies that have been carried out on the phenolic composition of the species of the genus Agave in order to show which species of the genus has been analyzed, what is the potential of the species of Agave as source of phenolics with important biological activities, and what is the significance of the phenolic profiles as chemotaxonomic markers in the genus. The results of the analysis of a dozen of Agave species reveal an important richness of phenolic compounds, including flavonoids, homoisoflavonoids, and phenolic acids, and a broad diversity of biological activities associated to those compounds, as antioxidant, antibacterial, antifungal, antinematod, and immunomodulatory capabilities. The phenols of Agave also have a major potential as specific chemotaxonomic markers.
文摘Adverse weather conditions and intensive use for fiber production have caused decline of natural populations of Agave lechuguilla. By these reasons, it is important to develop a sustainable management of this specie to avoid this situation. One of the first steps is to establish forest plantations as an alternative to restore A. lechuguilla habitat and increase its range while at the same time indirectly reducing erosion rates. The aim of this study was to identify the potential areas for establishment of plantations of A. lechuguilla in the State of Coahuila, Mexico. Areas for planting establishment were selected according to multi-criteria analysis using Geographic Information System (GIS) Idrisi32 Ver 2.0 and 3.2 Arc.View. The analysis was based on comparison of climate and soil requirements in contrast to environmental conditions of the tested area. The evaluated variables were: soil type, texture, depth, average annual temperature, annual precipitation, elevation and slope. Results derive in potential area maps, geo-referenced and their distribution by Agricultural Development District. An area of 5 million 159,273 hectares was determined with potential for establishment of A. lechuguilla forest plantations in the State of Coahuila. It was concluded that, use of GIS is a reliable tool to determine potential areas for A. lechuguilla forest plantations, where this specie has higher probability of success in survival and biomass production. This study will support programs aimed to strengthen the economy of rural producers and also recover and preserve the arid state.
文摘Here, we evaluated the possibility of growing grape tomatoes on three different mixtures of composted agave bagasse substrate. Tomatoes were grown in 28-L pots for 106 days under a drip irrigation system with a hydroponic crop nutrient solution. The average production of grape tomatoes was 338.9, 358.9, and 325.7 g/pot/cut for each of the substrates studied, and no significant difference was observed between treatments (p > 0.5). The mean of numbers of grape tomatoes were 34.6, 38.8, and 34.2/pot/cut for each of the substrates studied, and no significant difference was observed between treatments (p > 0.5). The mean weight of individual grape tomatoes was about 10.4 g for all of the substrates tested. These results confirmed the notion that a composted agave bagasse substrate could replace an expensive imported substrate. The remaining challenge is to produce agave bagasse substrate commercially, because to date, agave bagasse substrates have only been produced on a pilot scale for testing. Therefore, it is necessary to implement a stabilized process for producing agave bagasse at a commercial level. This process must consider economic production costs and market value to ensure that the product is competitive with other substrates typically used in soilless crops.
文摘Kel l ey Lee是一位年纪轻轻的主厨,她打造了上海几家最为知名、最受欢迎的餐厅（拳击猫Boxi n g Cat Brewery、龙舌兰小馆Cantina Agave和豆苗工坊Sproutworks）Kelley是一位极为要强的女商人,她在上海的10年职业生涯中开设了19家餐厅。她的最新餐饮概念一Liquid Laundry是一家具有纽约风味的美食酒吧,占地900平方米,现已成为在上海度过悠闲时光的热门场地。
文摘The blue agave (Agave tequilana Weber variety azul) is a species that is widely used to produce tequila. For this reason, in the last decade, large extensions of terrain have been established for its cultivation. However, much of this land has been abandoned in a short time because at the beginning it was not known that at least six years were needed before the agave could be harvested. However, when the growers wanted to reintegrate some of these crops into the productive process, their exact age and the cultural activities needed for harvesting the agave were unknown because information regarding its vegetative development relative to its age was lacking. The hypothesis of this work was: If the morphological properties reflect the development state of the population individuals, then the properties height, basal area, rosette diameter, the north, south, east and west leaf, leaves number and foliar verticil will help to characterize the age categories in Agave tequilana Weber variety azul population under cultivated conditions. To this end, six plots with plants of different ages (one to six years) were selected in the municipality of Llera, Tamaulipas, Mexico. In each plot, 60 individuals were selected at random, and their morphological properties (height, basal area, rosette diameter, northern leaf, southern leaf, eastern leaf, western leaf;number of leaves and leaf whorl) were measured. With these data, a principal component analysis was performed to determine which of these properties explains the most variation of the data. The results indicate that height and basal area are the properties that are most closely associated with plant age. It is also for the first time demonstrated that harvest time can be reduced by one or two years, which favors producers economically because it could generate savings in production costs.
文摘Somatic embryogenesis is a very efficient way to propagate economically important plants;however, not all genotypes within a species can be propagated using this method, as a combined effect of both genetic and epigenetic mechanisms may be involved in the response. The aim of the present study was to perform a comparative analysis of the genetic differences through amplified fragment length polymorphism (AFLP) and the epigenetic differences through methylation-sensitive amplified polymorphism (MSAP) of two Agave fourcroydes clonal lines, one highly embryogenic (K33) and the other non-embryogenic (K7). Genetic and epigenetic variabilities existed within each clonal line;however, the polymorphic profiles from the two marker systems allowed us to clearly distinguish the two clonal lines before somatic embryogenesis induction. During the induction, the changes detected were mainly 1) unmethylated fragments in the initial explants that were methylated during induction (methylation events) and 2) fragments with different methylation states in the initial explant that were unmethylated in some stages of the process (demethylation events). K33 showed greater dynamism in relation to methylation/demethylation events, while K7 presented the methylation events in a more constant range and at higher levels during all process.
文摘This paper presents an overview of different handling systems and use of the agave bagasse. These systems have appeared from different research works always taking in account the environmental sustainability. It is mentioned that the agave bagasse can be used for animal feeding, for the elaboration of compound materials, as an element for agricultural and hydroponic vegetables cultivation purposes, and also as a means to treat biosolids, vinasses, and bagasses of slaughterhouses and tanneries.
基金To CONACYT for the Master’s scholarship of Arnoldo Enrique Alfaro Corres(Scholarship No.621617).
文摘The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana L.In order to do the establishment of cells,the formation of callus was induced;for it two auxins were evaluated:2,4-dichlorophenoxyacetic acid(2,4-D)and 4-mino-3,5,6-trichloropicolinic acid(picloram)at three concentrations(0.25,0.5 and 0.75 mg L−1)in three explants(leaf,root and meristems)cultured in MS semisolid medium.The callogenesis response was related to the type and section of the explant,as well as the regulator used,and a cell suspension was established using 0.5 mg L−1 naphthaleneacetic acid(NAA)+0.5 mg L−1 Benzylaminopurine(BAP).Seedlings were exposed to polyethyleneglycol(15%and 30%w/v)with a water potential of−0.87 and−2.67 MPa,respectively,under soil conditions.Water stress was applied through restricted irrigation.Fresh weight,root system growth,and chlorophyll concentration were some of the parameters that were affected by the effect of water deficit on A.americana L.Chlorophyll concentration values were significantly decreased by 15 at 30%PEG(19.6 SPAD units)compared to the control treatment.In in vitro plants,the highest concentration of proline was found in the roots,being the treatment with 30%polyethylene glycol where the highest concentration of this osmoregulator was obtained(62.5 mg g−1 DW).Under restricted irrigation conditions,an increase in proline concentration was observed both in the aerial part(2.2μg 100 g−1 DW)and in the root system(1.8μg 100 g−1 DW).However,the concentrations found were approximately ten times greater,less than those found under in vitro conditions.Therefore,the accumulation of proline can be considered an indicator of stress in Agave Americana L.growth in vitro.
基金Instituto Politécnico Nacional(IPN)and Consejo Nacional de Ciencia y Tecnología(CONACYT)for financial support(Scholarship number:785612).
文摘The Agave genus has played a key role in the food security of communities at the local and regional level for millennia.The use and knowledge of these plants led to the development of the oldest agricultural centres in America.Therefore,a review of the literature on the ethnic and cultural particularities of the use of agave species in food and current knowledge on their nutritional contribution to the human diet is presented.56 species of agaves with food uses were registered by 55 ethnic groups in the American continent.Of this total,23 species also have a medicinal purpose.The number of agave species used by these ethnic groups shows an important agromorphological diversity.People collect and cultivate agaves to use their stem and foliar bases,the floral scape,the flowers and flower buds,the leaves,and the sap to prepare different foods.In many cases these foods help them survive in adverse conditions.The contribution of agaves to human nutrition has been studied from nutraceutical components such as saponins,inulin,fructans,phenolic compounds and antioxidants,dietary fiber,amino acids,and minerals.In the face of global climate change,agaves represent a strategic biological resource for the world,making their use,conservation and study a priority.Agave products will continue to show their health benefits.Human use of agaves aids diversification of the genus while contributing to the well-being and permanence of a variety of cultures.
