The tobacco whitefly,Bemisia tabaci,is a notorious pest affecting various crops globally,and it exhibits high levels of resistance to various insecticides.Afidopyropen is a recently commercialized pyropene insecticide...The tobacco whitefly,Bemisia tabaci,is a notorious pest affecting various crops globally,and it exhibits high levels of resistance to various insecticides.Afidopyropen is a recently commercialized pyropene insecticide for B.tabaci control with high selectivity and a novel mode of action.We previously identified a high level of afidopyropen resistance in a field-collected population after selection in the lab,and named it the HD-Afi strain.In the present study,minimal cross-resistance in the HD-Afi strain was found between afidopyropen and other common chemical agents.However,the P450 enzyme activity in HD-Afi was 2.18 times the level in susceptible strain HD-S.Expression analysis revealed that two of 12 candidate P450 genes,namely CYP6DW3 and CYP4C64,were significantly up-regulated in HD-Afi.Silencing CYP6DW3 and CYP4C64 by RNA interference(RNAi)substantially increased the susceptibility of whitefly adults,confirming their involvement in afidopyropen resistance.Homology modeling and molecular docking analyses demonstrated stable binding of afidopyropen to CYP6DW3 and CYP4C64,with binding free energies of–6.87 and–6.11 kcal mol^(-1),respectively.The findings of this study suggest that the induction of CYP6DW3 and CYP4C64 facilitates afidopyropen detoxification,contributing to the development of resistance in B.tabaci.展开更多
The cotton aphid,Aphis gossypii Glover(Hemiptera:Aphididae),is an important insect pest of cotton crops worldwide.The objectives of this study were to determine the acute toxicity of afidopyropen and whether there are...The cotton aphid,Aphis gossypii Glover(Hemiptera:Aphididae),is an important insect pest of cotton crops worldwide.The objectives of this study were to determine the acute toxicity of afidopyropen and whether there are any effects of sublethal concentrations of afidopyropen on the biological characteristics of A.gossypii.The results showed that afidopyropen possesses high acute toxicity to adult A.gossypii with a 72 h LC_(50) value of 1.062 mg L^(–1).A sublethal concentration(LC_(10))of afidopyropen significantly decreased adult longevity,fecundity and oviposition days of female adults in both F0 and F1 generations.The total pre-adult survival of F_(1) progeny was also significantly reduced by 30%at the LC_(10) of afidopyropen.In addition,the nymph developmental time,pre-adult period,adult pre-reproductive period(APRP),and total pre-reproductive period(TPRP)of the F1 progeny were significantly prolonged compared with the control.Several population parameters,including the net reproductive rate(R_(0)),intrinsic rate of increase(r)and finite rate of increase(λ)of F_(1) progeny were significantly decreased by a sublethal afidopyropen concentration exposure.These results indicated that sublethal concentration of afidopyropen can significantly suppress A.gossypii population growth.It would be useful for assessing the overall effects of afidopyropen on A.gossypii.展开更多
[目的]评价新型杀虫剂5%双丙环虫酯可分散液剂对黄瓜蚜虫的田间防效。[方法]分别于2016、2017年进行田间药效试验。[结果]5%双丙环虫酯可分散液剂7.5~12.5 g a.i./hm^2对黄瓜蚜虫防效显著,药后3 d防效达94.33%~98.32%,药后7 d防效为92.0...[目的]评价新型杀虫剂5%双丙环虫酯可分散液剂对黄瓜蚜虫的田间防效。[方法]分别于2016、2017年进行田间药效试验。[结果]5%双丙环虫酯可分散液剂7.5~12.5 g a.i./hm^2对黄瓜蚜虫防效显著,药后3 d防效达94.33%~98.32%,药后7 d防效为92.07%~96.20%,持效期可达14 d左右。[结论]5%双丙环虫酯可分散液剂可作为防治黄瓜蚜虫的高效低毒药剂应用推广。展开更多
基金partly supported by research grants from the Outstanding Youth Foundation of the Beijing Academy of Agriculture and Forestry Sciences,China(YXQN202301)the National Natural Science Foundation of China(32272522)the Beijing Natural Science Foundation,China(6232005)。
文摘The tobacco whitefly,Bemisia tabaci,is a notorious pest affecting various crops globally,and it exhibits high levels of resistance to various insecticides.Afidopyropen is a recently commercialized pyropene insecticide for B.tabaci control with high selectivity and a novel mode of action.We previously identified a high level of afidopyropen resistance in a field-collected population after selection in the lab,and named it the HD-Afi strain.In the present study,minimal cross-resistance in the HD-Afi strain was found between afidopyropen and other common chemical agents.However,the P450 enzyme activity in HD-Afi was 2.18 times the level in susceptible strain HD-S.Expression analysis revealed that two of 12 candidate P450 genes,namely CYP6DW3 and CYP4C64,were significantly up-regulated in HD-Afi.Silencing CYP6DW3 and CYP4C64 by RNA interference(RNAi)substantially increased the susceptibility of whitefly adults,confirming their involvement in afidopyropen resistance.Homology modeling and molecular docking analyses demonstrated stable binding of afidopyropen to CYP6DW3 and CYP4C64,with binding free energies of–6.87 and–6.11 kcal mol^(-1),respectively.The findings of this study suggest that the induction of CYP6DW3 and CYP4C64 facilitates afidopyropen detoxification,contributing to the development of resistance in B.tabaci.
基金supported by the National Natural Science Foundation of China(31801760 and 31871997)the Fundamental Research Funds for the Central Universities,China(2662019QD052)。
文摘The cotton aphid,Aphis gossypii Glover(Hemiptera:Aphididae),is an important insect pest of cotton crops worldwide.The objectives of this study were to determine the acute toxicity of afidopyropen and whether there are any effects of sublethal concentrations of afidopyropen on the biological characteristics of A.gossypii.The results showed that afidopyropen possesses high acute toxicity to adult A.gossypii with a 72 h LC_(50) value of 1.062 mg L^(–1).A sublethal concentration(LC_(10))of afidopyropen significantly decreased adult longevity,fecundity and oviposition days of female adults in both F0 and F1 generations.The total pre-adult survival of F_(1) progeny was also significantly reduced by 30%at the LC_(10) of afidopyropen.In addition,the nymph developmental time,pre-adult period,adult pre-reproductive period(APRP),and total pre-reproductive period(TPRP)of the F1 progeny were significantly prolonged compared with the control.Several population parameters,including the net reproductive rate(R_(0)),intrinsic rate of increase(r)and finite rate of increase(λ)of F_(1) progeny were significantly decreased by a sublethal afidopyropen concentration exposure.These results indicated that sublethal concentration of afidopyropen can significantly suppress A.gossypii population growth.It would be useful for assessing the overall effects of afidopyropen on A.gossypii.
