In the face of carbon,nitrogen,and phosphorus starvation,microorganisms have evolved adaptive mechanisms to maintain growth.In a previous study,we identified a protein predicted to contain acylCoA-binding domains in t...In the face of carbon,nitrogen,and phosphorus starvation,microorganisms have evolved adaptive mechanisms to maintain growth.In a previous study,we identified a protein predicted to contain acylCoA-binding domains in the plant pathogenic fungus Verticillium dahliae.The predicted protein,designated VdAcb1,possesses an atypical signal peptide.However,the functions of this acyl-CoA-binding protein in V.dahliae are not clear.In this research,in vivo or in vitro assays confirmed that VdAcb1 is secreted extracellularly from V.dahliae,although it does not have the typical signal peptide.Furthermore,the unconventional secretion of VdAcb1 was dependent on VdGRASP,a member of the compartment for unconventional protein secretion(CUPS).The deletion mutant strain of VdAcb1(DVdAcb1)exhibited significant sensitivity to carbon starvation.RNA-seq revealed that the expression of genes related to filamentous growth(MSB2 pathway)and sugar transport were regulated by VdAcb1 under conditions of carbon starvation.Yeast one-hybrid experiments further showed that the expression of VdAcb1 was positively regulated by the transcription factor VdMsn4.The DVdAcb1 strain showed significantly reduced virulence on Gossypium hirsutum and Nicotiana benthamiana.We hypothesize that under conditions of carbon starvation,the expression of VdAcb1 is activated by VdMsn4 and VdAcb1 is secreted into the extracellular space.In turn,this activates the downstream MAPK pathway to enhance filamentous growth and virulence of V.dahliae.展开更多
Acyl-CoA-binding proteins(ACBPs)are important for the transport of acyl groups for macro molecular biosynthesis involved in plant growth,development,and diverse stress(e.g.,cold,drought,salinity,and heavy metals)respo...Acyl-CoA-binding proteins(ACBPs)are important for the transport of acyl groups for macro molecular biosynthesis involved in plant growth,development,and diverse stress(e.g.,cold,drought,salinity,and heavy metals)responses.Here,we report the phylogeny and characteristics of the ACBP family in the woody plant Populus trichocarpa.Eight genes encoding ACBP proteins were identified,and they are distributed on eight chromosomes in P.trichocarpa.These PtACBP genes were divided into four subgroups according to gene structure,conserved motifs and phylogenetic relationship.Promoter analysis revealed that cis-elements were related to stress response,phytohormone response,and physical and reproductive growth regulation.Expression levels of PtACBP genes varied among different organs,with the highest expression in leaves and the lowest in stems.Quantitative real-time PCR(qRT-PCR)analysis showed that under salinity-alkali stresses(i.e.,200 mM NaCl,75 mM Na2CO3,and 100 mM NaHCO3),four(PtACBP1,PtACBP3,PtACBP4 and PtACBP8)of eight PtACBP genes were significantly induced in roots and leaves.These data provide a comprehensive analysis of the ACBPs family in P.trichocarpa,which could be useful for gene function analyses.展开更多
基金supported by the National Key Research and Development Program of China(2022YFE0130800,2022YFD1400300)the National Natural Science Foundation of China(32370213,32302327)+1 种基金Shandong Provincial Natural Science Foundation(ZR2020MC115)the Agricultural Sciences Talent Program CAAS to JYC,the Agricultural Science and Technology Innovation Program grant to J-YC.
文摘In the face of carbon,nitrogen,and phosphorus starvation,microorganisms have evolved adaptive mechanisms to maintain growth.In a previous study,we identified a protein predicted to contain acylCoA-binding domains in the plant pathogenic fungus Verticillium dahliae.The predicted protein,designated VdAcb1,possesses an atypical signal peptide.However,the functions of this acyl-CoA-binding protein in V.dahliae are not clear.In this research,in vivo or in vitro assays confirmed that VdAcb1 is secreted extracellularly from V.dahliae,although it does not have the typical signal peptide.Furthermore,the unconventional secretion of VdAcb1 was dependent on VdGRASP,a member of the compartment for unconventional protein secretion(CUPS).The deletion mutant strain of VdAcb1(DVdAcb1)exhibited significant sensitivity to carbon starvation.RNA-seq revealed that the expression of genes related to filamentous growth(MSB2 pathway)and sugar transport were regulated by VdAcb1 under conditions of carbon starvation.Yeast one-hybrid experiments further showed that the expression of VdAcb1 was positively regulated by the transcription factor VdMsn4.The DVdAcb1 strain showed significantly reduced virulence on Gossypium hirsutum and Nicotiana benthamiana.We hypothesize that under conditions of carbon starvation,the expression of VdAcb1 is activated by VdMsn4 and VdAcb1 is secreted into the extracellular space.In turn,this activates the downstream MAPK pathway to enhance filamentous growth and virulence of V.dahliae.
基金supported by grants from the Fundamental Research Funds for the Central Universities(Nos.2572018B03)the College Students Innovations Special Project funded by NEFU(No.202010225173)+1 种基金the Fundamental Research Funds for the Central Universities(Nos.2572019CT03)the Natural Science Foundation of Heilongjiang Province(No.ZD2019C003)。
文摘Acyl-CoA-binding proteins(ACBPs)are important for the transport of acyl groups for macro molecular biosynthesis involved in plant growth,development,and diverse stress(e.g.,cold,drought,salinity,and heavy metals)responses.Here,we report the phylogeny and characteristics of the ACBP family in the woody plant Populus trichocarpa.Eight genes encoding ACBP proteins were identified,and they are distributed on eight chromosomes in P.trichocarpa.These PtACBP genes were divided into four subgroups according to gene structure,conserved motifs and phylogenetic relationship.Promoter analysis revealed that cis-elements were related to stress response,phytohormone response,and physical and reproductive growth regulation.Expression levels of PtACBP genes varied among different organs,with the highest expression in leaves and the lowest in stems.Quantitative real-time PCR(qRT-PCR)analysis showed that under salinity-alkali stresses(i.e.,200 mM NaCl,75 mM Na2CO3,and 100 mM NaHCO3),four(PtACBP1,PtACBP3,PtACBP4 and PtACBP8)of eight PtACBP genes were significantly induced in roots and leaves.These data provide a comprehensive analysis of the ACBPs family in P.trichocarpa,which could be useful for gene function analyses.
