[Objective]The aim was to explore the active compound of Morus alba leaves with the analysis of active components of hexane extractives of M.alba leaves.[Method]Antibacterial assays of hexane extractives of M.alba lea...[Objective]The aim was to explore the active compound of Morus alba leaves with the analysis of active components of hexane extractives of M.alba leaves.[Method]Antibacterial assays of hexane extractives of M.alba leaves were done and the volatile components of hexane extractives of M.alba leaves with the strongest antibacterial activity were analyzed by gas chromatography-mass spectrometry（GC-MS）.[Result]The results of inhibitory effect of hexane extractives of M.alba leaves on Staphylococcus aureus and Escherichia coli showed that hexane extractives of M.alba leaves collected in June,July and August had antibacterial activity against S.aureus and had no antibacterial effect against E.coli.Furthermore,hexane extractives of M.alba leaves collected in June had the strongest antibacterial activity against S.aureus（inhibition diameter 10.95 mm）.The hexane extractives of M.alba leaves collected in June was analyzed by GC-MS,the main volatile components were tetradecane（16.76%）,dodecane（13.20%）,diisobutyl phthalate（10.26%）,decane（9.10%）,hexadecane（8.71%）,linolenyl alcohol（7.25%）,octadecane（5.88%）,eicosane（3.26%）,dibutyl phthalate（2.59%）.[Conclusion]Linolenyl alcohol was the potential antibacterial compound.展开更多
AIM: To investigate the active constituents of Lignum Sappan(Caesalpinia sappan L.) on growth-related signaling and cell mitosis. METHOD: The influence of the ethyl acetate(EtOAc) extract of Lignum Sappan and its cons...AIM: To investigate the active constituents of Lignum Sappan(Caesalpinia sappan L.) on growth-related signaling and cell mitosis. METHOD: The influence of the ethyl acetate(EtOAc) extract of Lignum Sappan and its constituents on growth-related signaling were evaluated by a luciferase assay in cells stably- transfected with NF-κB, STAT1, or STAT3 responsive luciferase reporter plasmid. The inhibitory effect on the cell cycle was determined by flow cytometric analysis. The anti-tumor activities were assessed in vitro and in vivo. RESULTS: The EtOAc extract of Lignum Sappan had inhibitory activities on growth-related signaling and cell mitosis. Three major active compounds were sappanchalcone, brazilin, and butein. Sappanchalcone blocked cell cycle progression in the G2/M phase, brazilin inhibited TNFα/NF-κB signaling, while butein inhibited IL-6/STAT3 signaling, as well as TNFα/NF-κB signaling. The three compounds all demonstrated cytotoxic activities against human tumor cells in vitro. In a S180 tumor cell-bearing mice model, the anti-tumor efficacy of the EtOAc extract was better than the individual compounds acting alone. CONCLUSION: These results indicate that Lignum Sappan contains multiple active compounds with different antitumor activities, which act synergistically to enhance their anti-tumor effects. The EtOAc extract of Lignum Sappan may be better than individual active constituent as a novel medicine for the treatment of cancer.展开更多
INTRODUCTION:Withania somnifera(W.somnifera)is a plant with remarkable pharmacological properties.The plant has an impressive profile of medicinal uses in the folk medicine system of several civilizations.AIM:This com...INTRODUCTION:Withania somnifera(W.somnifera)is a plant with remarkable pharmacological properties.The plant has an impressive profile of medicinal uses in the folk medicine system of several civilizations.AIM:This comprehensive study is aimed to characterize phytochemicals in fruit of W.somnifera and tested for in vitro anticancer potential to find out active candidate in disease prevention and treatment.METHODS:The bioactive components from W.somnifera fruit were extracted with polar and non-polar solvents.Anticancer potential of the isolated bioactive was assessed against different cancer cell lines through MTT assay and Incucytes imaging analysis.The extracts were characterized for secondary metabolites using GCMS(gas chromatography-mass spectrometer),LCMS(liquid(electrospray Ionization)and 1H-NMR(electrospray Ionization)techniques.RESULTS:Both freeze-dried and rotary evaporator condensed extracts exhibited anticancer potential against MDA-MB-231,MCF7-SKOV3 and SKBR3 cell lines.The tested extracts have cell growth inhibition potential against mammalian cancer cell line.Hexacosanedioic acid purified from n-hexane extract through HPLC was investigated for its cytotoxicity against breast cancer cell line SKBR3 by using Incucytes imaging analysis.Conclusion:We found that a variety of bioactive compounds existed in this plant.One identified compound that was not investigated for cytotoxicity in previous studies was purified and its application showed cytotoxicity on breast cancer cell lines.A number of bioactive identified from W.somnifera fruit may have an effective potential for development into chemotherapy drugs.展开更多
Human beings have consumed lemon(Citrus limon)and lime(Citrus aurantifolia or Citrus latiflia)for thousands of years.Among the variety of citrus families,lemon and lime are originated from the hybridization of citron ...Human beings have consumed lemon(Citrus limon)and lime(Citrus aurantifolia or Citrus latiflia)for thousands of years.Among the variety of citrus families,lemon and lime are originated from the hybridization of citron with primitive papeda,hence they are similar from the nutritional and organoleptic standpoints,whereas very different from other citrus species such as orange and mandarin.Except for fresh produce,a signifi cant percentage of lemon and lime are processed and separated as juice,essential oils,pulps and other products.Lemon and lime juice or fruit itself is rich in vitamins,minerals and flavonoids which are rich sources for human nutrition.Consumption of lemon and lime fruit or juice are benefi cial for human health in the scope of urinary citrate increase,oxidative stress relief,improvement in lipid profi les and infl ammation markers,neuroprotective effects among others.These beneficial effects of lemon and lime are not only because of their high vitamin C content but also other bioactive micronutrients such as fl avonoids.Essential oils from lemon and lime have fresh and zesty aroma for perfumery and flavor applications for centuries.Compared with orange or mandarin,the integrated review for lemon and lime dietary bioactive compounds and essential oils is scarce.Therefore,in this review,we introduced the historical cultivation,consumption and process of lemon and lime,discussed the chemical and biological activities of phytochemicals in lemon and lime fruits and juice,and summarized volatile and non-volatile components in lemon and lime oil.This review may provide a comprehensive perspective for entire lemon and lime industry as well as their scientifi c values.展开更多
Objective: Exposure to certain stresses in small doses might lead to a protective effect by improving resistance to other stressors. Dead Sea (DS) minerals can be a relevant source to induce positive stress due to the...Objective: Exposure to certain stresses in small doses might lead to a protective effect by improving resistance to other stressors. Dead Sea (DS) minerals can be a relevant source to induce positive stress due to their high salinity and unique mineral combination. This concept could be further optimized using advanced unique cell biotechnology. The purpose of this study was to elucidate the innovative concept of DS minerals (water extract and black mud) supplementation in small amount to Pichia pastoris yeast growth media as a positive stress by testing the capability of accepted fermentation compounds to affect the appearance of skin. Methods: Skin equivalents were topically applied with different Pichia pastoris fermentations (Metabiotics?). Skin elasticity biomarkers were tested, since loss of elasticity and suppleness is a natural skin aging process leading to deeper wrinkles and loss of firmness. A preliminary screening at the gene level using DNA microarray was performed and subsequently, the following proteins were detected using ELISA or immunoblotting assays: elastin, fibulin-1, lysyl oxidase (LOX), metalloproteinase 3 (MMP-3), E-cadherin, claudin 4, tight junction protein (TJP)-1 and TJP-2. UVB irradiation was selected as a stressor. Results: Fermentation compounds generated in the presence of small doses of DS minerals affected the expression of various elasticity-related genes in skin. Moreover, they significantly attenuated the abnormal UVB-induced alterations, the proteins elastin, fibulin-1, LOX, MMP-3, E-cadherin and TJP-2. Conclusions: The observations clearly demonstrate that when DS Metabiotics? compounds are topically applied, significant alterations in several biomarkers that contribute to skin elasticity occur. Thus, these novel compounds have the potential to serve as skincare actives.展开更多
Jamun(Syzygium cumini Skeels)is a nutritious fruit and has historically been used as both as an edible and a traditional medicine.The jamun seed is an inedible by-product of the fruit.However,their high concentration ...Jamun(Syzygium cumini Skeels)is a nutritious fruit and has historically been used as both as an edible and a traditional medicine.The jamun seed is an inedible by-product of the fruit.However,their high concentration of phytochemicals makes them valuable components of nutraceuticals.Various studies on diverse biological activities of jamun seeds have warranted their application in human health and biomedical fields.This review paper discusses critically the phytochemical composition of jamun seeds and the bioactivities,including their antioxidant,anti-inflammatory,anti-cancer,antidiabetic,anti-microbial,anti-obesity,hepato-protective,cardio and gastro-protective properties.The bioactivity of jamun seed is related to the presence of phenols,flavonoids,steroids,alkaloids,triterpenoids,tannins,and saponins.Animal research in vivo,and in vitro experiments with human and animal cell models support the idea that jamun seed extract can be valuable additions in food and biomedical fields due to the diverse bio-functional properties.However,in-depth and systematic in vivo clinical trials using human subjects must be conducted to confirm the safe consumption limit and establish other therapeutic roles of jamun seeds for their abundant utilization as a nutraceutical or pharmacological component.Moreover,research is required to understand the exact mechanisms of the bioactivities exhibited by jamun seeds.展开更多
Crude extracts of Alpinia conchigera a species from the Malaysian Ginger (Zingiberaceae) family and its fractions obtained from various extraction methods were assayed for melanogenesis inhibition activity and cell vi...Crude extracts of Alpinia conchigera a species from the Malaysian Ginger (Zingiberaceae) family and its fractions obtained from various extraction methods were assayed for melanogenesis inhibition activity and cell viability. The crude extract obtained from the ethanolic extraction and the super critical fluid extraction did not exhibit significant melanin inhibition activity and was shown to be toxic to the melanocyte cells in comparison to the water extract. The crude aqueous extracts displayed melanin inhibition of 96.38 ± 1.60% and cell viability 109.90 ± 8.32% at a concentration of 500 μg/mL. Bioassay guided fractionation was performed on the water extracts to isolate the active compounds. The actives were identified as trans-cinnamaldehyde and chavicol glucopyranoside with both compound showing potent anti-melanogenesis activity. At 4.9 μg/ml, both trans-cinnamaldehyde and chavicol glucopyranoside gave 85% inhibition of melanin formation in vitro with 77% and 97% cell viability respectively. In comparison, kojic acid, a known skin lightening agent showed 90.0% inhibition at 100 μg/mL. The bioactive composition comprising the extract, active fraction, purified compounds or mixture thereof of Alpinia conchigera may be used for cosmetic and pharmaceutical applications, particularly for the purposes of reducing skin pigmentation.展开更多
The valorization of Amazonian wood residues into active chemical compounds could be an eco-friendly,cost-effective and valuable way to develop wood preservative formulations to enhance the decay and termite resistance...The valorization of Amazonian wood residues into active chemical compounds could be an eco-friendly,cost-effective and valuable way to develop wood preservative formulations to enhance the decay and termite resistance of low-durable wood species.Wacapou(Vouacapoua americana.,Fabaceae)is a well-known Guianese wood spe-cies commonly used in local wood construction due to its outstanding natural durability,which results from the presence of a large panel of extractives compounds.In addition,its industrial processing generates large amounts of residues.Wacapou residues were extracted by maceration using four different solvents(water/ethanol,ethyl acetate,hexane and dichloromethane/methanol),separately and successively.The yield of each extractive fraction was determined,and their chemical compositions were analyzed by Liquid Chromatography-Mass Spectrometry(LC-MS).Ethyl acetate led to the highest extraction yield,and the active compounds were identified in the obtained extractive fraction.In this sense,the fungicidal and termite-repellent properties of these extractives were then tested using a screening laboratory(with temperate and tropical microorganisms),according to the solution concentration(1%,2.5%,5%,8%and 10%).Finally,Virola michelii Heckel wood samples(low durable species)were impregnated with the 8%concentration solution.The impregnated wood samples were then exposed to a soil bed test.The results highlighted that the nature of the solvent used during wood maceration affects the con-tent of the obtained extractive fractions.Ultra-Performance Liquid Chromatography–High-Resolution Mass Spectrometry(UHPLC-HRMS)analyses showed the influence of extraction parameters on the nature of the extracted molecules.Wacapou extracts(from ethyl acetate maceration)showed good anti-fungal and anti-termite activities.Additionally,the concentration in extractives had an impact on the anti-termite activity level for Reti-culitermesflavipes and Cryptotermes sp.Formulations based on Wacapou extractives showed a good potential for valorization in eco-friendly preservatives,aiming to confer better durability to local low-durability wood species.展开更多
Methane(CH_(4))has a higher heat capacity(104.9 kcal/mol)than carbon dioxide(CO_(2)),and this has inspired research aimed at reducing methane levels to retard global warming.Hydroxylation under ambient conditions thro...Methane(CH_(4))has a higher heat capacity(104.9 kcal/mol)than carbon dioxide(CO_(2)),and this has inspired research aimed at reducing methane levels to retard global warming.Hydroxylation under ambient conditions through methanotrophs can provide crucial information for understanding the harsh C-H activation of methane.Soluble methane monooxygenase(sMMO)belongs to the bacterial multi-component monooxygenase superfamily and requires hydroxylase(MMOH),regulatory(MMOB),and reductase(MMOR)components.Recent structural and biophysical studies have demonstrated that these components accelerate and retard methane hydroxylation in MMOH through protein-protein interactions.Complex structures of sMMO,including MMOH-MMOB and MMOH-MMOD,illustrate how these regulatory and inhibitory components orchestrate the di-iron active sites located within the four-helix bundles of MMOH,specifically at the docking surface known as the canyon region.In addition,recent biophysical studies have demonstrated the role of MmoR,aσ54-dependent transcriptional regulator,in regulating sMMO expression.This perspective article introduces remarkable discoveries in recent reports on sMMO components that are crucial for understanding sMMO expression and activities.Our findings provide insight into how sMMO components interact with MMOH to control methane hydroxylation,shedding light on the mechanisms governing sMMO expression and the interactions between activating enzymes and promoters.展开更多
Patients with complete spinal cord injury retain the potential for volitional muscle activity in muscles located below the spinal injury level.However,because of prolonged inactivity,initial attempts to activate these...Patients with complete spinal cord injury retain the potential for volitional muscle activity in muscles located below the spinal injury level.However,because of prolonged inactivity,initial attempts to activate these muscles may not effectively engage any of the remaining neurons in the descending pathway.A previous study unexpectedly found that a brief clinical round of passive activity significantly increased volitional muscle activation,as measured by surface electromyography.In this study,we further explored the effect of passive activity on surface electromyographic signals during volitional control tasks among individuals with complete spinal cord injury.Eleven patients with chronic complete thoracic spinal cord injury were recruited.Surface electromyography data from eight major leg muscles were acquired and compared before and after the passive activity protocol.The results indicated that the passive activity led to an increased number of activated volitional muscles and an increased frequency of activation.Although the cumulative root mean square of surface electromyography amplitude for volitional control of movement showed a slight increase after passive activity,the difference was not statistically significant.These findings suggest that brief passive activity may enhance the ability to initiate volitional muscle activity during surface electromyography tasks and underscore the potential of passive activity for improving residual motor control among patients with motor complete spinal cord injury.展开更多
Microglia are present throughout the central nervous system and are vital in neural repair,nutrition,phagocytosis,immunological regulation,and maintaining neuronal function.In a healthy spinal cord,microglia are accou...Microglia are present throughout the central nervous system and are vital in neural repair,nutrition,phagocytosis,immunological regulation,and maintaining neuronal function.In a healthy spinal cord,microglia are accountable for immune surveillance,however,when a spinal cord injury occurs,the microenvironment drastically changes,leading to glial scars and failed axonal regeneration.In this context,microglia vary their gene and protein expression during activation,and proliferation in reaction to the injury,influencing injury responses both favorably and unfavorably.A dynamic and multifaceted injury response is mediated by microglia,which interact directly with neurons,astrocytes,oligodendrocytes,and neural stem/progenitor cells.Despite a clear understanding of their essential nature and origin,the mechanisms of action and new functions of microglia in spinal cord injury require extensive research.This review summarizes current studies on microglial genesis,physiological function,and pathological state,highlights their crucial roles in spinal cord injury,and proposes microglia as a therapeutic target.展开更多
To expand the study on the structures and biological activities of the anthracyclines anticancer drugs and reduce their toxic side effects,the new anthraquinone derivatives,9‑pyridylanthrahydrazone(9‑PAH)and 9,10‑bisp...To expand the study on the structures and biological activities of the anthracyclines anticancer drugs and reduce their toxic side effects,the new anthraquinone derivatives,9‑pyridylanthrahydrazone(9‑PAH)and 9,10‑bispyridylanthrahydrazone(9,10‑PAH)were designed and synthesized.Utilizing 9‑PAH and 9,10‑PAH as promising anticancer ligands,their respective copper complexes,namely[Cu(L1)Cl_(2)]Cl(1)and{[Cu_(4)(μ_(2)‑Cl)_(3)Cl_(4)(9,10‑PAH)_(2)(DMSO)_(2)]Cl_(2)}_(n)(2),were subsequently synthesized,where the new ligand L1 is formed by coupling two 9‑PAH ligands in the coordination reaction.The chemical and crystal structures of 1 and 2 were elucidated by IR,MS,elemental analysis,and single‑crystal X‑ray diffraction.Complex 1 forms a mononuclear structure.L1 coordinates with Cu through its three N atoms,together with two Cl atoms,to form a five‑coordinated square pyramidal geometry.Complex 2 constitutes a polymeric structure,wherein each structural unit centrosymmetrically encompasses two five‑coordinated binuclear copper complexes(Cu1,Cu2)of 9,10‑PAH,with similar square pyramidal geometry.A chlorine atom(Cl_(2)),located at the symmetry center,bridges Cu1 and Cu1A to connect the two binuclear copper structures.Meanwhile,the two five‑coordinated Cu2 atoms symmetrically bridge the adjacent structural units via one coordinated Cl atom,respectively,thus forming a 1D chain‑like polymeric structure.In vitro anticancer activity assessments revealed that 1 and 2 showed significant cytotoxicity even higher than cisplatin.Specifically,the IC_(50)values of 2 against HeLa‑229 and SK‑OV‑3 cancer cell lines were determined to be(5.92±0.32)μmol·L^(-1)and(6.48±0.39)μmol·L^(-1),respectively.2 could also block the proliferation of HeLa‑229 cells in S phase and significantly induce cell apoptosis.In addition,fluorescence quenching competition experiments suggested that 2 might interact with DNA by an intercalative binding mode,offering insights into its underlying anticancer mechanism.CCDC:2388918,1;2388919,2.展开更多
Corrosion activities and related accidents are significant issues for marine facilities,leading to considerable economic losses.Waterborne epoxy(EP)coating has been seen as one of the optimal options for corrosion pro...Corrosion activities and related accidents are significant issues for marine facilities,leading to considerable economic losses.Waterborne epoxy(EP)coating has been seen as one of the optimal options for corrosion protection due to its stable properties and eco-friendliness(0 g/L volatile organic compounds).Nevertheless,several intrinsic deficiencies require improvement,such as fragile mechanical properties and defects(macro and micro),resulting in the continuous deterioration of comprehensive coating performances.In this work,a novel nanocomposite coating with mechanical enhancement,intelligent self-reporting,and active protection is fabricated by integrating the functionalized and compatible graphene oxide/cerium based metal-organic framework multiscale structure(GO-CeMOF-P/M).Notably,the homogenous dispersion of GO-CeMOF-P/M and its chemical interaction with the polymer matrix effectively reduces the defects resulting from solution volatilizing and enhances the compactness,which boosts the tensile strength(32.1 MPa/8.5%)and dry adhesion force(5.8 MPa)of the coating.Additionally,the controllable responsiveness and release of multiscale nanocomposite within external environments endow intelligent active protection and self-reporting characteristics for the GO-CeMOF-P/M-EP coating,making it especially suitable for a variety of practical marine applications.Furthermore,following immersion of 80 d in the aggressive environment,Zf=0.01 Hz value of GO-CeMOF-P/M-EP coating is 1.2×10^(10)Ωcm^(2),which is 164.4 times larger than that of EP coating(7.3×10^(7)Ωcm^(2)),demonstrating remarkably strengthened anti-corrosion ability.Consequently,by offering an intriguing design strategy,the current work anticipates addressing the inherent deficiencies of EP coating and facilitating its practicality and feasibility in real sea environments.展开更多
Sulfide-based all-solid-state lithium batteries(ASSLBs) with nickel-rich oxide cathodes are emerging as primary contenders for the next generation rechargeable batteries,owing to their superior safety and energy densi...Sulfide-based all-solid-state lithium batteries(ASSLBs) with nickel-rich oxide cathodes are emerging as primary contenders for the next generation rechargeable batteries,owing to their superior safety and energy density.However,the all-solid-state batteries with nickel-rich oxide cathodes suffer from performance degradation due to the reactions between the highly reactive surface oxygen of the cathode and the electrolyte,as well as the instability of the bulk oxygen structure in the cathode.Herein,we propose a synergistic modification design scheme to adjust the oxygen activity from surface to bulk.The LiBO_(2)coating inhibits the reactivity of surface lattice oxygen ions.Meanwhile,Zr doping in the bulk phase forms strong Zr-O covalent bonds that stabilize the bulk lattice oxygen structure.The synergistic effect of these modifications prevents the release of oxygen,thus avoiding the degradation of the cathode/SE interface.Additionally,the regulation of surface-to-bulk oxygen activity establishes a highly stable interface,thereby enhancing the lithium ion diffusion kinetics and mechanical stability of the cathode.Consequently,cathodes modified with this synergistic strategy exhibit outstanding performance in sulfide-based ASSLBs,including an ultra-long cycle life of 100,000 cycles,ultra-high rate capability at 45C,and 85% high active material content in the composite cathode.Additionally,ASSLB exhibits stable cycling under high loading conditions of 82.82 mg cm^(-2),achieving an areal capacity of 17.90 mA h cm^(-2).These encouraging results pave the way for practical applications of ASSLBs in fast charging,long cycle life,and high energy density in the future.展开更多
To explore the potential utilization of Elaeagnus mollis,we conducted a comprehensive assessment of its phytochemical composition,antioxidant properties,cholinesterase inhibition,and anti-HepG2 cell proliferation acti...To explore the potential utilization of Elaeagnus mollis,we conducted a comprehensive assessment of its phytochemical composition,antioxidant properties,cholinesterase inhibition,and anti-HepG2 cell proliferation activity across different plant parts(branch wood,branch bark,and pericarp)using various solvents(water,methanol,ethanol,and n-hexane).Our findings revealed that water extracts displayed superior antioxidant activities in ABTS and RP assays,while methanol extracts exhibited better performance in DPPH and FRAP assays.Moreover,methanol extracts demonstrated the highest effectiveness against anti-HepG2 cell proliferation,whereas n-hexane extracts showed greater efficiency in cholinesterase inhibition.Notably,branch bark extracts exhibited the highest levels of phytochemical compounds,with both branch bark and pericarp extracts demonstrating significant effects in cholinesterase inhibition and anti-HepG2 cell proliferation.Correlation analysis indicated that phytochemical compounds were primarily responsible for the observed biological activities.Overall,extracts from the branch bark and pericarp of E.mollis showed promising potential for antioxidant and anticancer activities,suggesting their suitability for applications in the pharmaceutical industry as health-promoting products.展开更多
1.Introduction Changes in land use are key factors promoting global climate change,and the side effects of mining activity that destroy the soil,vegetation,and biodiversity lead to imbalanced carbon cycling in terrest...1.Introduction Changes in land use are key factors promoting global climate change,and the side effects of mining activity that destroy the soil,vegetation,and biodiversity lead to imbalanced carbon cycling in terrestrial ecosystems.展开更多
Thirteen novel diterpenoids,comprising seven tiglianes(walliglianes G−M,1−7),four rhamnofolanes(wallinofolanes A−D,8−11),and two daphnanes(wallaphnanes A and B,12 and 13),together with two known rhamnofolane diterpeno...Thirteen novel diterpenoids,comprising seven tiglianes(walliglianes G−M,1−7),four rhamnofolanes(wallinofolanes A−D,8−11),and two daphnanes(wallaphnanes A and B,12 and 13),together with two known rhamnofolane diterpenoids(euphorwallside H and euphorwallside I,14 and 15),were isolated and characterized from Euphorbia wallichii(E.wallichii).The chemical structures of these compounds were elucidated through nuclear magnetic resonance(NMR),mass spectrometry(MS),and quantum chemical calculations.Compounds 9 and 11 demonstrated protective effects against H2O2-induced BV-2 microglial cell damage.Molecular docking analyses indicated that compound 9 exhibited binding affinity to the anti-oxidant-related targets HMGCR,GSTP1,and SHBG.展开更多
The ongoing revolution in information technology is reshaping human life. In the realm of health behavior, wearable technology emerges as a leading digital solution,capturing physical behaviors (i.e., physical activit...The ongoing revolution in information technology is reshaping human life. In the realm of health behavior, wearable technology emerges as a leading digital solution,capturing physical behaviors (i.e., physical activity, sedentary habits, sleep patterns) within the 24-h cycle of daily life. Wearables are applied in research, clinical practice, and as lifestyle devices;most obvious, they promise to be a key element for increasing human physical activity, one of the biggest health challenges nowadays.展开更多
Reproductive hormones associated with the hypothalamic-pituitary-gonadal(HPG)axis are closely linked to bone homeostasis.In this study,we demonstrate that Gonadotropin inhibitory hormone(GnIH,one of the key reproducti...Reproductive hormones associated with the hypothalamic-pituitary-gonadal(HPG)axis are closely linked to bone homeostasis.In this study,we demonstrate that Gonadotropin inhibitory hormone(GnIH,one of the key reproductive hormones upstream of the HPG axis)plays an indispensable role in regulating bone homeostasis and maintaining bone mass.We find that deficiency of GnIH or its receptor Gpr147 leads to a significant reduction in bone mineral density(BMD)in mice primarily by enhancement of osteoclast activation in vivo and in vitro.Mechanistically,GnIH/Gpr147 inhibits osteoclastogenesis by the PI3K/AKT,MAPK,NF-κB and Nfatc1 signaling pathways.Furthermore,GnIH treatment was able to alleviate bone loss in aging,ovariectomy(OVX)or LPS-induced mice.Moreover,the therapy using green light promotes the release of GnIH and rescues OVX-induced bone loss.In humans,serum GnIH increases and bone resorption markers decrease after green light exposure.Therefore,our study elucidates that GnIH plays an important role in maintaining bone homeostasis via modulating osteoclast differentiation and demonstrates the potential of GnIH therapy or green light therapy in preventing osteoporosis.展开更多
Catalytic oxidation of organic pollutants is a well-known and effective technique for pollutant abatement.Unfortunately,this method is significantly hindered in practical applications by the lowefficiency and difficul...Catalytic oxidation of organic pollutants is a well-known and effective technique for pollutant abatement.Unfortunately,this method is significantly hindered in practical applications by the lowefficiency and difficult recovery of the catalysts in a powdery form.Herein,a three-dimensional(3D)framework of Fe-incorporated Ni_(3)S_(2)nanosheets in-situ grown on Ni foam(Fe-Ni_(3)S_(2)@NF)was fabricated by a facile two-step hydrothermal process and applied to trigger peroxymonosulfate(PMS)oxidation of organic compounds inwater.A homogeneous growth environment enabled the uniform and scalable growth of Fe-Ni_(3)S_(2)nanosheets on the Ni foam.Fe-Ni_(3)S_(2)@NF possessed outstanding activity and durability in activating PMS,as it effectively facilitated electron transfer from organic pollutants to PMS.Fe-Ni_(3)S_(2)@NF initially supplied electrons to PMS,causing the catalyst to undergo oxidation,and subsequently accepted electrons from organic compounds,returning to its initial state.The introduction of Fe into the Ni_(3)S_(2)lattice enhanced electrical conductivity,promoting mediated electron transfer between PMS and organic compounds.The 3D conductive Ni foam provided an ideal platform for the nucleation and growth of Fe-Ni_(3)S_(2),accelerating pollutant abatement due to its porous structure and high conductivity.Furthermore,its monolithic nature simplified the catalyst recycling process.A continuous flow packed-bed reactor by encapsulating Fe-Ni_(3)S_(2)@NF catalyst achieved complete pollutant abatement with continuous operation for 240 h,highlighting its immense potential for practical environmental remediation.This study presents a facile synthesis method for creating a novel type of monolithic catalyst with high activity and durability for decontamination through Fenton-like processes.展开更多
基金Supported by the Forestry Department of Heilongjiang Province Science and Technology Promotion Project(01043208003)the Project of Science and Technology Department of Heilongjiang Province(LC07C27)~~
文摘[Objective]The aim was to explore the active compound of Morus alba leaves with the analysis of active components of hexane extractives of M.alba leaves.[Method]Antibacterial assays of hexane extractives of M.alba leaves were done and the volatile components of hexane extractives of M.alba leaves with the strongest antibacterial activity were analyzed by gas chromatography-mass spectrometry（GC-MS）.[Result]The results of inhibitory effect of hexane extractives of M.alba leaves on Staphylococcus aureus and Escherichia coli showed that hexane extractives of M.alba leaves collected in June,July and August had antibacterial activity against S.aureus and had no antibacterial effect against E.coli.Furthermore,hexane extractives of M.alba leaves collected in June had the strongest antibacterial activity against S.aureus（inhibition diameter 10.95 mm）.The hexane extractives of M.alba leaves collected in June was analyzed by GC-MS,the main volatile components were tetradecane（16.76%）,dodecane（13.20%）,diisobutyl phthalate（10.26%）,decane（9.10%）,hexadecane（8.71%）,linolenyl alcohol（7.25%）,octadecane（5.88%）,eicosane（3.26%）,dibutyl phthalate（2.59%）.[Conclusion]Linolenyl alcohol was the potential antibacterial compound.
基金supported by the China National Natural Science Foundation Grant(Nos.9112970181102848+1 种基金31129004)the China National Science and Technology 973 grant(No.2012CB910704)
文摘AIM: To investigate the active constituents of Lignum Sappan(Caesalpinia sappan L.) on growth-related signaling and cell mitosis. METHOD: The influence of the ethyl acetate(EtOAc) extract of Lignum Sappan and its constituents on growth-related signaling were evaluated by a luciferase assay in cells stably- transfected with NF-κB, STAT1, or STAT3 responsive luciferase reporter plasmid. The inhibitory effect on the cell cycle was determined by flow cytometric analysis. The anti-tumor activities were assessed in vitro and in vivo. RESULTS: The EtOAc extract of Lignum Sappan had inhibitory activities on growth-related signaling and cell mitosis. Three major active compounds were sappanchalcone, brazilin, and butein. Sappanchalcone blocked cell cycle progression in the G2/M phase, brazilin inhibited TNFα/NF-κB signaling, while butein inhibited IL-6/STAT3 signaling, as well as TNFα/NF-κB signaling. The three compounds all demonstrated cytotoxic activities against human tumor cells in vitro. In a S180 tumor cell-bearing mice model, the anti-tumor efficacy of the EtOAc extract was better than the individual compounds acting alone. CONCLUSION: These results indicate that Lignum Sappan contains multiple active compounds with different antitumor activities, which act synergistically to enhance their anti-tumor effects. The EtOAc extract of Lignum Sappan may be better than individual active constituent as a novel medicine for the treatment of cancer.
基金the IRSIP Scholarship Program of Higher Education commission of Pakistan for funding。
文摘INTRODUCTION:Withania somnifera(W.somnifera)is a plant with remarkable pharmacological properties.The plant has an impressive profile of medicinal uses in the folk medicine system of several civilizations.AIM:This comprehensive study is aimed to characterize phytochemicals in fruit of W.somnifera and tested for in vitro anticancer potential to find out active candidate in disease prevention and treatment.METHODS:The bioactive components from W.somnifera fruit were extracted with polar and non-polar solvents.Anticancer potential of the isolated bioactive was assessed against different cancer cell lines through MTT assay and Incucytes imaging analysis.The extracts were characterized for secondary metabolites using GCMS(gas chromatography-mass spectrometer),LCMS(liquid(electrospray Ionization)and 1H-NMR(electrospray Ionization)techniques.RESULTS:Both freeze-dried and rotary evaporator condensed extracts exhibited anticancer potential against MDA-MB-231,MCF7-SKOV3 and SKBR3 cell lines.The tested extracts have cell growth inhibition potential against mammalian cancer cell line.Hexacosanedioic acid purified from n-hexane extract through HPLC was investigated for its cytotoxicity against breast cancer cell line SKBR3 by using Incucytes imaging analysis.Conclusion:We found that a variety of bioactive compounds existed in this plant.One identified compound that was not investigated for cytotoxicity in previous studies was purified and its application showed cytotoxicity on breast cancer cell lines.A number of bioactive identified from W.somnifera fruit may have an effective potential for development into chemotherapy drugs.
基金supported by Hubei Science and Technology Plan Key Project(G2019ABA100).
文摘Human beings have consumed lemon(Citrus limon)and lime(Citrus aurantifolia or Citrus latiflia)for thousands of years.Among the variety of citrus families,lemon and lime are originated from the hybridization of citron with primitive papeda,hence they are similar from the nutritional and organoleptic standpoints,whereas very different from other citrus species such as orange and mandarin.Except for fresh produce,a signifi cant percentage of lemon and lime are processed and separated as juice,essential oils,pulps and other products.Lemon and lime juice or fruit itself is rich in vitamins,minerals and flavonoids which are rich sources for human nutrition.Consumption of lemon and lime fruit or juice are benefi cial for human health in the scope of urinary citrate increase,oxidative stress relief,improvement in lipid profi les and infl ammation markers,neuroprotective effects among others.These beneficial effects of lemon and lime are not only because of their high vitamin C content but also other bioactive micronutrients such as fl avonoids.Essential oils from lemon and lime have fresh and zesty aroma for perfumery and flavor applications for centuries.Compared with orange or mandarin,the integrated review for lemon and lime dietary bioactive compounds and essential oils is scarce.Therefore,in this review,we introduced the historical cultivation,consumption and process of lemon and lime,discussed the chemical and biological activities of phytochemicals in lemon and lime fruits and juice,and summarized volatile and non-volatile components in lemon and lime oil.This review may provide a comprehensive perspective for entire lemon and lime industry as well as their scientifi c values.
文摘Objective: Exposure to certain stresses in small doses might lead to a protective effect by improving resistance to other stressors. Dead Sea (DS) minerals can be a relevant source to induce positive stress due to their high salinity and unique mineral combination. This concept could be further optimized using advanced unique cell biotechnology. The purpose of this study was to elucidate the innovative concept of DS minerals (water extract and black mud) supplementation in small amount to Pichia pastoris yeast growth media as a positive stress by testing the capability of accepted fermentation compounds to affect the appearance of skin. Methods: Skin equivalents were topically applied with different Pichia pastoris fermentations (Metabiotics?). Skin elasticity biomarkers were tested, since loss of elasticity and suppleness is a natural skin aging process leading to deeper wrinkles and loss of firmness. A preliminary screening at the gene level using DNA microarray was performed and subsequently, the following proteins were detected using ELISA or immunoblotting assays: elastin, fibulin-1, lysyl oxidase (LOX), metalloproteinase 3 (MMP-3), E-cadherin, claudin 4, tight junction protein (TJP)-1 and TJP-2. UVB irradiation was selected as a stressor. Results: Fermentation compounds generated in the presence of small doses of DS minerals affected the expression of various elasticity-related genes in skin. Moreover, they significantly attenuated the abnormal UVB-induced alterations, the proteins elastin, fibulin-1, LOX, MMP-3, E-cadherin and TJP-2. Conclusions: The observations clearly demonstrate that when DS Metabiotics? compounds are topically applied, significant alterations in several biomarkers that contribute to skin elasticity occur. Thus, these novel compounds have the potential to serve as skincare actives.
文摘Jamun(Syzygium cumini Skeels)is a nutritious fruit and has historically been used as both as an edible and a traditional medicine.The jamun seed is an inedible by-product of the fruit.However,their high concentration of phytochemicals makes them valuable components of nutraceuticals.Various studies on diverse biological activities of jamun seeds have warranted their application in human health and biomedical fields.This review paper discusses critically the phytochemical composition of jamun seeds and the bioactivities,including their antioxidant,anti-inflammatory,anti-cancer,antidiabetic,anti-microbial,anti-obesity,hepato-protective,cardio and gastro-protective properties.The bioactivity of jamun seed is related to the presence of phenols,flavonoids,steroids,alkaloids,triterpenoids,tannins,and saponins.Animal research in vivo,and in vitro experiments with human and animal cell models support the idea that jamun seed extract can be valuable additions in food and biomedical fields due to the diverse bio-functional properties.However,in-depth and systematic in vivo clinical trials using human subjects must be conducted to confirm the safe consumption limit and establish other therapeutic roles of jamun seeds for their abundant utilization as a nutraceutical or pharmacological component.Moreover,research is required to understand the exact mechanisms of the bioactivities exhibited by jamun seeds.
文摘Crude extracts of Alpinia conchigera a species from the Malaysian Ginger (Zingiberaceae) family and its fractions obtained from various extraction methods were assayed for melanogenesis inhibition activity and cell viability. The crude extract obtained from the ethanolic extraction and the super critical fluid extraction did not exhibit significant melanin inhibition activity and was shown to be toxic to the melanocyte cells in comparison to the water extract. The crude aqueous extracts displayed melanin inhibition of 96.38 ± 1.60% and cell viability 109.90 ± 8.32% at a concentration of 500 μg/mL. Bioassay guided fractionation was performed on the water extracts to isolate the active compounds. The actives were identified as trans-cinnamaldehyde and chavicol glucopyranoside with both compound showing potent anti-melanogenesis activity. At 4.9 μg/ml, both trans-cinnamaldehyde and chavicol glucopyranoside gave 85% inhibition of melanin formation in vitro with 77% and 97% cell viability respectively. In comparison, kojic acid, a known skin lightening agent showed 90.0% inhibition at 100 μg/mL. The bioactive composition comprising the extract, active fraction, purified compounds or mixture thereof of Alpinia conchigera may be used for cosmetic and pharmaceutical applications, particularly for the purposes of reducing skin pigmentation.
基金PROTEXTWOOD (ID 2202-102) funded through LabEx AGRO ANR-10-LABX-0001-01 (under ISiteUniversité de Montpellier framework)the project PANTHER2-Guyane funded through AgenceNationale de la Recherche (ANR-22-CE43-0019)+2 种基金“Investissement d’Avenir” grant managed by Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-25-01)supported by the FEDER (European Regional Development Fund)research project “EcovaloBois” (Project number: GY0015430)by the CNRS peps INSIS2018 research project “GuyavaloFibres”.
文摘The valorization of Amazonian wood residues into active chemical compounds could be an eco-friendly,cost-effective and valuable way to develop wood preservative formulations to enhance the decay and termite resistance of low-durable wood species.Wacapou(Vouacapoua americana.,Fabaceae)is a well-known Guianese wood spe-cies commonly used in local wood construction due to its outstanding natural durability,which results from the presence of a large panel of extractives compounds.In addition,its industrial processing generates large amounts of residues.Wacapou residues were extracted by maceration using four different solvents(water/ethanol,ethyl acetate,hexane and dichloromethane/methanol),separately and successively.The yield of each extractive fraction was determined,and their chemical compositions were analyzed by Liquid Chromatography-Mass Spectrometry(LC-MS).Ethyl acetate led to the highest extraction yield,and the active compounds were identified in the obtained extractive fraction.In this sense,the fungicidal and termite-repellent properties of these extractives were then tested using a screening laboratory(with temperate and tropical microorganisms),according to the solution concentration(1%,2.5%,5%,8%and 10%).Finally,Virola michelii Heckel wood samples(low durable species)were impregnated with the 8%concentration solution.The impregnated wood samples were then exposed to a soil bed test.The results highlighted that the nature of the solvent used during wood maceration affects the con-tent of the obtained extractive fractions.Ultra-Performance Liquid Chromatography–High-Resolution Mass Spectrometry(UHPLC-HRMS)analyses showed the influence of extraction parameters on the nature of the extracted molecules.Wacapou extracts(from ethyl acetate maceration)showed good anti-fungal and anti-termite activities.Additionally,the concentration in extractives had an impact on the anti-termite activity level for Reti-culitermesflavipes and Cryptotermes sp.Formulations based on Wacapou extractives showed a good potential for valorization in eco-friendly preservatives,aiming to confer better durability to local low-durability wood species.
基金This research was supported by"Regional Innovation Strategy"(2023RIS-008)and"C1 Gas Refinery Program"(NRF-2015M3D3D3A1A01064876)through the National Research Foundation of Koreafunded by the Ministry of Education(NRF-2017R1A6A1A03015876).
文摘Methane(CH_(4))has a higher heat capacity(104.9 kcal/mol)than carbon dioxide(CO_(2)),and this has inspired research aimed at reducing methane levels to retard global warming.Hydroxylation under ambient conditions through methanotrophs can provide crucial information for understanding the harsh C-H activation of methane.Soluble methane monooxygenase(sMMO)belongs to the bacterial multi-component monooxygenase superfamily and requires hydroxylase(MMOH),regulatory(MMOB),and reductase(MMOR)components.Recent structural and biophysical studies have demonstrated that these components accelerate and retard methane hydroxylation in MMOH through protein-protein interactions.Complex structures of sMMO,including MMOH-MMOB and MMOH-MMOD,illustrate how these regulatory and inhibitory components orchestrate the di-iron active sites located within the four-helix bundles of MMOH,specifically at the docking surface known as the canyon region.In addition,recent biophysical studies have demonstrated the role of MmoR,aσ54-dependent transcriptional regulator,in regulating sMMO expression.This perspective article introduces remarkable discoveries in recent reports on sMMO components that are crucial for understanding sMMO expression and activities.Our findings provide insight into how sMMO components interact with MMOH to control methane hydroxylation,shedding light on the mechanisms governing sMMO expression and the interactions between activating enzymes and promoters.
基金supported by the Fundamental Research Funds for Central Public Welfare Research Institute,No.2020CZ-5(to WS and GS)the National Natural Science Foundation of China,No.31970970(to JSR)Fundamental Research Funds for the Central Universities,No.YWF-23-YG-QB-010(to JSR)。
文摘Patients with complete spinal cord injury retain the potential for volitional muscle activity in muscles located below the spinal injury level.However,because of prolonged inactivity,initial attempts to activate these muscles may not effectively engage any of the remaining neurons in the descending pathway.A previous study unexpectedly found that a brief clinical round of passive activity significantly increased volitional muscle activation,as measured by surface electromyography.In this study,we further explored the effect of passive activity on surface electromyographic signals during volitional control tasks among individuals with complete spinal cord injury.Eleven patients with chronic complete thoracic spinal cord injury were recruited.Surface electromyography data from eight major leg muscles were acquired and compared before and after the passive activity protocol.The results indicated that the passive activity led to an increased number of activated volitional muscles and an increased frequency of activation.Although the cumulative root mean square of surface electromyography amplitude for volitional control of movement showed a slight increase after passive activity,the difference was not statistically significant.These findings suggest that brief passive activity may enhance the ability to initiate volitional muscle activity during surface electromyography tasks and underscore the potential of passive activity for improving residual motor control among patients with motor complete spinal cord injury.
文摘Microglia are present throughout the central nervous system and are vital in neural repair,nutrition,phagocytosis,immunological regulation,and maintaining neuronal function.In a healthy spinal cord,microglia are accountable for immune surveillance,however,when a spinal cord injury occurs,the microenvironment drastically changes,leading to glial scars and failed axonal regeneration.In this context,microglia vary their gene and protein expression during activation,and proliferation in reaction to the injury,influencing injury responses both favorably and unfavorably.A dynamic and multifaceted injury response is mediated by microglia,which interact directly with neurons,astrocytes,oligodendrocytes,and neural stem/progenitor cells.Despite a clear understanding of their essential nature and origin,the mechanisms of action and new functions of microglia in spinal cord injury require extensive research.This review summarizes current studies on microglial genesis,physiological function,and pathological state,highlights their crucial roles in spinal cord injury,and proposes microglia as a therapeutic target.
文摘To expand the study on the structures and biological activities of the anthracyclines anticancer drugs and reduce their toxic side effects,the new anthraquinone derivatives,9‑pyridylanthrahydrazone(9‑PAH)and 9,10‑bispyridylanthrahydrazone(9,10‑PAH)were designed and synthesized.Utilizing 9‑PAH and 9,10‑PAH as promising anticancer ligands,their respective copper complexes,namely[Cu(L1)Cl_(2)]Cl(1)and{[Cu_(4)(μ_(2)‑Cl)_(3)Cl_(4)(9,10‑PAH)_(2)(DMSO)_(2)]Cl_(2)}_(n)(2),were subsequently synthesized,where the new ligand L1 is formed by coupling two 9‑PAH ligands in the coordination reaction.The chemical and crystal structures of 1 and 2 were elucidated by IR,MS,elemental analysis,and single‑crystal X‑ray diffraction.Complex 1 forms a mononuclear structure.L1 coordinates with Cu through its three N atoms,together with two Cl atoms,to form a five‑coordinated square pyramidal geometry.Complex 2 constitutes a polymeric structure,wherein each structural unit centrosymmetrically encompasses two five‑coordinated binuclear copper complexes(Cu1,Cu2)of 9,10‑PAH,with similar square pyramidal geometry.A chlorine atom(Cl_(2)),located at the symmetry center,bridges Cu1 and Cu1A to connect the two binuclear copper structures.Meanwhile,the two five‑coordinated Cu2 atoms symmetrically bridge the adjacent structural units via one coordinated Cl atom,respectively,thus forming a 1D chain‑like polymeric structure.In vitro anticancer activity assessments revealed that 1 and 2 showed significant cytotoxicity even higher than cisplatin.Specifically,the IC_(50)values of 2 against HeLa‑229 and SK‑OV‑3 cancer cell lines were determined to be(5.92±0.32)μmol·L^(-1)and(6.48±0.39)μmol·L^(-1),respectively.2 could also block the proliferation of HeLa‑229 cells in S phase and significantly induce cell apoptosis.In addition,fluorescence quenching competition experiments suggested that 2 might interact with DNA by an intercalative binding mode,offering insights into its underlying anticancer mechanism.CCDC:2388918,1;2388919,2.
基金financially supported by the National Natural Science Foundation of China(Nos.52371088,52071347,and U20A20233)Guangdong Basic and Applied Basic Research Foundation(No.2023A1515240007)Shenzhen Science and Technology Program(No.KJZD20230923114819041).
文摘Corrosion activities and related accidents are significant issues for marine facilities,leading to considerable economic losses.Waterborne epoxy(EP)coating has been seen as one of the optimal options for corrosion protection due to its stable properties and eco-friendliness(0 g/L volatile organic compounds).Nevertheless,several intrinsic deficiencies require improvement,such as fragile mechanical properties and defects(macro and micro),resulting in the continuous deterioration of comprehensive coating performances.In this work,a novel nanocomposite coating with mechanical enhancement,intelligent self-reporting,and active protection is fabricated by integrating the functionalized and compatible graphene oxide/cerium based metal-organic framework multiscale structure(GO-CeMOF-P/M).Notably,the homogenous dispersion of GO-CeMOF-P/M and its chemical interaction with the polymer matrix effectively reduces the defects resulting from solution volatilizing and enhances the compactness,which boosts the tensile strength(32.1 MPa/8.5%)and dry adhesion force(5.8 MPa)of the coating.Additionally,the controllable responsiveness and release of multiscale nanocomposite within external environments endow intelligent active protection and self-reporting characteristics for the GO-CeMOF-P/M-EP coating,making it especially suitable for a variety of practical marine applications.Furthermore,following immersion of 80 d in the aggressive environment,Zf=0.01 Hz value of GO-CeMOF-P/M-EP coating is 1.2×10^(10)Ωcm^(2),which is 164.4 times larger than that of EP coating(7.3×10^(7)Ωcm^(2)),demonstrating remarkably strengthened anti-corrosion ability.Consequently,by offering an intriguing design strategy,the current work anticipates addressing the inherent deficiencies of EP coating and facilitating its practicality and feasibility in real sea environments.
基金financially supported by the National Natural Science Foundation of China (52474338,22109084 and 52304338)the Hunan Provincial Key Research and Development Program (2024JK2093,2023GK2016)supported in part by the High Performance Computing Center of Central South University.
文摘Sulfide-based all-solid-state lithium batteries(ASSLBs) with nickel-rich oxide cathodes are emerging as primary contenders for the next generation rechargeable batteries,owing to their superior safety and energy density.However,the all-solid-state batteries with nickel-rich oxide cathodes suffer from performance degradation due to the reactions between the highly reactive surface oxygen of the cathode and the electrolyte,as well as the instability of the bulk oxygen structure in the cathode.Herein,we propose a synergistic modification design scheme to adjust the oxygen activity from surface to bulk.The LiBO_(2)coating inhibits the reactivity of surface lattice oxygen ions.Meanwhile,Zr doping in the bulk phase forms strong Zr-O covalent bonds that stabilize the bulk lattice oxygen structure.The synergistic effect of these modifications prevents the release of oxygen,thus avoiding the degradation of the cathode/SE interface.Additionally,the regulation of surface-to-bulk oxygen activity establishes a highly stable interface,thereby enhancing the lithium ion diffusion kinetics and mechanical stability of the cathode.Consequently,cathodes modified with this synergistic strategy exhibit outstanding performance in sulfide-based ASSLBs,including an ultra-long cycle life of 100,000 cycles,ultra-high rate capability at 45C,and 85% high active material content in the composite cathode.Additionally,ASSLB exhibits stable cycling under high loading conditions of 82.82 mg cm^(-2),achieving an areal capacity of 17.90 mA h cm^(-2).These encouraging results pave the way for practical applications of ASSLBs in fast charging,long cycle life,and high energy density in the future.
基金National Natural Science Foundation of China(Grant No.31600549).
文摘To explore the potential utilization of Elaeagnus mollis,we conducted a comprehensive assessment of its phytochemical composition,antioxidant properties,cholinesterase inhibition,and anti-HepG2 cell proliferation activity across different plant parts(branch wood,branch bark,and pericarp)using various solvents(water,methanol,ethanol,and n-hexane).Our findings revealed that water extracts displayed superior antioxidant activities in ABTS and RP assays,while methanol extracts exhibited better performance in DPPH and FRAP assays.Moreover,methanol extracts demonstrated the highest effectiveness against anti-HepG2 cell proliferation,whereas n-hexane extracts showed greater efficiency in cholinesterase inhibition.Notably,branch bark extracts exhibited the highest levels of phytochemical compounds,with both branch bark and pericarp extracts demonstrating significant effects in cholinesterase inhibition and anti-HepG2 cell proliferation.Correlation analysis indicated that phytochemical compounds were primarily responsible for the observed biological activities.Overall,extracts from the branch bark and pericarp of E.mollis showed promising potential for antioxidant and anticancer activities,suggesting their suitability for applications in the pharmaceutical industry as health-promoting products.
基金supported by the National Natural Science Foun dation of China(52374170 and 51974313)the National Key Research and Development Plan Project(2022YFF1303300).
文摘1.Introduction Changes in land use are key factors promoting global climate change,and the side effects of mining activity that destroy the soil,vegetation,and biodiversity lead to imbalanced carbon cycling in terrestrial ecosystems.
基金supported by the National Natural Science Foundation of China(Nos.82405016 and 82141216)the Project of Frontier Technology Platform for Research Projects of Liaoning Provincial Department of Education in 2024(No.LJ232410163056)+3 种基金"Select the best candidates to lead key research projects"of Fujian University of Traditional Chinese Medicine(Nos.XJB2022008 and XJB2023001)the Foundation of Fujian University of Traditional Chinese Medicine(Nos.X2023001-Talent and X2024002-Talent)the Young and Middle-aged Teacher Education Research Project in Fujian Province(No.JZ230023)the Central Government Guides Local Science and Technology Development Fund Projects(No.2023L3014).
文摘Thirteen novel diterpenoids,comprising seven tiglianes(walliglianes G−M,1−7),four rhamnofolanes(wallinofolanes A−D,8−11),and two daphnanes(wallaphnanes A and B,12 and 13),together with two known rhamnofolane diterpenoids(euphorwallside H and euphorwallside I,14 and 15),were isolated and characterized from Euphorbia wallichii(E.wallichii).The chemical structures of these compounds were elucidated through nuclear magnetic resonance(NMR),mass spectrometry(MS),and quantum chemical calculations.Compounds 9 and 11 demonstrated protective effects against H2O2-induced BV-2 microglial cell damage.Molecular docking analyses indicated that compound 9 exhibited binding affinity to the anti-oxidant-related targets HMGCR,GSTP1,and SHBG.
基金funded in part by the German Research Foundation(Grant reference:496846758).
文摘The ongoing revolution in information technology is reshaping human life. In the realm of health behavior, wearable technology emerges as a leading digital solution,capturing physical behaviors (i.e., physical activity, sedentary habits, sleep patterns) within the 24-h cycle of daily life. Wearables are applied in research, clinical practice, and as lifestyle devices;most obvious, they promise to be a key element for increasing human physical activity, one of the biggest health challenges nowadays.
基金National Key Research and Development Program of China(2023YFB3810200 to J.L.)National Natural Science Foundation of China(92168204,82225030 to J.L.)Fundamental Research Funds for the Central Universities(22120210586 to J.L.)。
文摘Reproductive hormones associated with the hypothalamic-pituitary-gonadal(HPG)axis are closely linked to bone homeostasis.In this study,we demonstrate that Gonadotropin inhibitory hormone(GnIH,one of the key reproductive hormones upstream of the HPG axis)plays an indispensable role in regulating bone homeostasis and maintaining bone mass.We find that deficiency of GnIH or its receptor Gpr147 leads to a significant reduction in bone mineral density(BMD)in mice primarily by enhancement of osteoclast activation in vivo and in vitro.Mechanistically,GnIH/Gpr147 inhibits osteoclastogenesis by the PI3K/AKT,MAPK,NF-κB and Nfatc1 signaling pathways.Furthermore,GnIH treatment was able to alleviate bone loss in aging,ovariectomy(OVX)or LPS-induced mice.Moreover,the therapy using green light promotes the release of GnIH and rescues OVX-induced bone loss.In humans,serum GnIH increases and bone resorption markers decrease after green light exposure.Therefore,our study elucidates that GnIH plays an important role in maintaining bone homeostasis via modulating osteoclast differentiation and demonstrates the potential of GnIH therapy or green light therapy in preventing osteoporosis.
基金supported by the National Natural Science Foundation of China(No.21876039)Y.Yao acknowledges the scholarship support from the China Scholarship Council(No.202106695010)Partial support from the Australian Research Council for DP230102406 is also acknowledged.
文摘Catalytic oxidation of organic pollutants is a well-known and effective technique for pollutant abatement.Unfortunately,this method is significantly hindered in practical applications by the lowefficiency and difficult recovery of the catalysts in a powdery form.Herein,a three-dimensional(3D)framework of Fe-incorporated Ni_(3)S_(2)nanosheets in-situ grown on Ni foam(Fe-Ni_(3)S_(2)@NF)was fabricated by a facile two-step hydrothermal process and applied to trigger peroxymonosulfate(PMS)oxidation of organic compounds inwater.A homogeneous growth environment enabled the uniform and scalable growth of Fe-Ni_(3)S_(2)nanosheets on the Ni foam.Fe-Ni_(3)S_(2)@NF possessed outstanding activity and durability in activating PMS,as it effectively facilitated electron transfer from organic pollutants to PMS.Fe-Ni_(3)S_(2)@NF initially supplied electrons to PMS,causing the catalyst to undergo oxidation,and subsequently accepted electrons from organic compounds,returning to its initial state.The introduction of Fe into the Ni_(3)S_(2)lattice enhanced electrical conductivity,promoting mediated electron transfer between PMS and organic compounds.The 3D conductive Ni foam provided an ideal platform for the nucleation and growth of Fe-Ni_(3)S_(2),accelerating pollutant abatement due to its porous structure and high conductivity.Furthermore,its monolithic nature simplified the catalyst recycling process.A continuous flow packed-bed reactor by encapsulating Fe-Ni_(3)S_(2)@NF catalyst achieved complete pollutant abatement with continuous operation for 240 h,highlighting its immense potential for practical environmental remediation.This study presents a facile synthesis method for creating a novel type of monolithic catalyst with high activity and durability for decontamination through Fenton-like processes.
