When a spiral groove is formed using superplastic molding, precision casting, additive manufacturing, or other non?mechanical processing technology, it is diffcult to meet the molding precision required for direct use...When a spiral groove is formed using superplastic molding, precision casting, additive manufacturing, or other non?mechanical processing technology, it is diffcult to meet the molding precision required for direct use, and the surface quality and accuracy of the shape need to be improved through a finishing process. In view of the poor reachability of the current tool?based polishing process, a tool?less polishing method using free?abrasive grains for complex spiral grooves is proposed. With this method, by controlling the movement of the workpiece, the design basis and relative motion of the abrasive particles along a helical path remain consistent, resulting in a better polishing profile. A spiral groove of a revolving body is taken as the research object; the influence of the installation method and the position of the parts, as well as the effect of the rotational speed of the abrasive ball on its relative motion along a helical trajectory, are studied, and the polishing cutting process of an abrasive ball is reasonably simplified. A consistent math?ematical model of the trajectory of an abrasive ball relative to the design helix is constructed. The grooved drum parts are verified through a polishing experiment. The spiral groove of the revolving body is modified and polished. Experiments show that the process not only corrects the shape a spiral groove error, but also reduces the surface roughness of a spiral groove. This study provides a theoretical basis for achieving free?abrasive, tool?free polishing.展开更多
Aiming to ensure the consistency of quality control of Traditional Chinese Medicines(TCMs),a combination method of high-performance liquid chromatography(HPLC),ultraviolet(UV),electrochemical(EC)was developed in this ...Aiming to ensure the consistency of quality control of Traditional Chinese Medicines(TCMs),a combination method of high-performance liquid chromatography(HPLC),ultraviolet(UV),electrochemical(EC)was developed in this study to comprehensively evaluate the quality of Antiviral Mixture(AM),and Comprehensive Linear Quantification Fingerprint Method(CLQFM)was used to process the data.Quantitative analysis of three active substances in TCM was conducted.A fivewavelength fusion fingerprint(FWFF)was developed,using second-order derivatives of UV spectral data to differentiate sample levels effectively.The combination of HPLC and UV spectrophotometry,along with electrochemical fingerprinting(ECFP),successfully evaluated total active substances.Ultimately,a multidimensional profiling analytical system for TCM was developed.展开更多
This paper presents a new optimization study of the placement and size of a photovoltaic source(PVS)in a distribution grid,based on annual records of meteorological parameters(irradiance,temperature).Based on the reco...This paper presents a new optimization study of the placement and size of a photovoltaic source(PVS)in a distribution grid,based on annual records of meteorological parameters(irradiance,temperature).Based on the recorded data,the production output as well as the daily average power(24-h vector)of the PVS is extracted over the year.When a power vector is available,it can be used as an input when searching for the optimal size of the PVS.This allows to take into account the constraint of the variation of the power generated by this source considering the variation of the power consumed by the electrical loads during the whole day.A multi-objective fitness function has been considered.The latter minimizes the active losses and maximizes the voltage stability index during the day,while considering the constraints of the system,that is,the security,technical,geographical,and meteorological constraints.This problem was solved using the Non-dominated Sorting Genetic Algorithm NSGA-II optimization technique under MATLAB 2021.It was applied to the distribution network of Ghardaïa of 59 nodes.展开更多
On the one hand,the separation of thousands of compounds in a complex extract is thrilling,but may be still be separated unsatisfactorily.Hence,the question arises where to stop in high-sophisticated separation scienc...On the one hand,the separation of thousands of compounds in a complex extract is thrilling,but may be still be separated unsatisfactorily.Hence,the question arises where to stop in high-sophisticated separation science?Which technical effort is economically justifiable in routine?On the other hand,the separation itself does not imply an effect-directed answer to questions such展开更多
The emergence rate and vitality of maize are directly affected by the sowing depth,and the uniformity of this depth is an important performance indicator of a planter,while the effective soil surface height informatio...The emergence rate and vitality of maize are directly affected by the sowing depth,and the uniformity of this depth is an important performance indicator of a planter,while the effective soil surface height information acquisition is the prerequisite for ensuring the accuracy of sowing depth control.The soil surface height variation acquisition system of a precision corn planter often produces profiling errors when performing active profiling due to interference from ground debris.In this study,a multipoint soil surface height variation information acquisition system was investigated,which consists of a ranging sensor group and a microcontroller unit(MCU)using a data comparison and screening method.The structure and specifications of the ranging sensors were determined according to the soil surface height variation and debris size,and a nonessential profiling control program was developed.Performed tests on the information acquisition system indicated that the measurement accuracy of the system was 3 mm,and when advancing at a speed of 8 km/h,the accuracy of the profiling decision and the system stability were 97.1%and 94.1%,respectively,indicating that the system was capable of nonessential profile control.The designed ranging system could provide a reference for the design of a ground information acquisition system of precision planters with an active profiling mechanism.展开更多
Gram‐negative bacteria are particularly prone to developing antimicrobial resistance(AMR),as evidenced by the WHO's ESKAPEE list of high‐priority pathogens.One strategy that has increased is the use of antibioti...Gram‐negative bacteria are particularly prone to developing antimicrobial resistance(AMR),as evidenced by the WHO's ESKAPEE list of high‐priority pathogens.One strategy that has increased is the use of antibiotic enhancers,which can reempower abandoned or poorly active antibiotics against the resistant strain of interest.In this study,the polyamino‐isoprenyl antibiotic enhancer,NV716,was tested in combination with two families of multi‐target Ser/Cys‐based enzyme inhibitors,the oxadiazolone derivatives(OX)and the Cyclipostins and Cyclophostin analogs(CyC),which are inactive against Gram‐negative ESKAPEE bacteria,to potentiate their antibacterial activity and thus make them active against these bacteria.We demonstrated that NV716 potentiates some OX and CyC compounds by permeabilizing the outer membrane and thus by increasing the inhibitor accumulation,as shown by fluorescence microscopy.By using the click‐chemistry activity‐based protein profiling(ABPP)approach coupled with proteomic analysis,we also confirmed the multi‐target nature of the best OX and CyC inhibitors by identifying their target proteins on a bacterial culture of Enterobacter cloacae.Remarkably,a large set of these identified proteins had already been captured in previous ABPP experiments conducted on Mycobacterium tuberculosis and/or Mycobacterium abscessus culture.Furthermore,we showed that five of the identified target proteins were present in a total lysate of Pseudomonas aeruginosa.Importantly,these latter enzymes are highly conserved among Gram‐negative bacteria,with two of them annotated as essential for bacterial survival.These results provide proof of concept that both OX and CyC,if successfully potentiated,could be used against ESKAPEE Gram‐negative bacteria.展开更多
Plant b-1,3-glucanases are members of the pathogenesis-related protein 2（PR-2） family,which is one of the 17 PR protein families and plays important roles in biotic and abiotic stress responses.One of the differenti...Plant b-1,3-glucanases are members of the pathogenesis-related protein 2（PR-2） family,which is one of the 17 PR protein families and plays important roles in biotic and abiotic stress responses.One of the differentially expressed proteins（spot 842） identified in a recent proteomic comparison between five pairs of closely related maize（Zea mays L.） lines differing in aflatoxin resistance was further investigated in the present study.Here,the corresponding cDNA was cloned from maize and designated as ZmGns.ZmGns encodes a protein of338 amino acids containing a potential signal peptide.The expression of Zm Gns was detectible in all tissues studied with the highest level in silks.ZmGns was significantly induced by biotic stresses including three bacteria and the fungus Aspergillus flavus.ZmGns was also induced by most abiotic stresses tested and growth hormones including salicylic acid.In vivo,ZmGns showed a significant inhibitory activity against thebacterial pathogen Pseudomonas syringae pv.tomato DC3000 and fungal pathogen Botrytis cinerea when it overexpressed in Arabidopsis.Its high level of expression in the silk tissue and its induced expression by phytohormone treatment,as well as by bacterial and fungal infections,suggest it plays a complex role in maize growth,development,and defense.展开更多
基金Supported by Key Scientific&Technological Research Project of Henan Province,China(Grant No.102102210497)
文摘When a spiral groove is formed using superplastic molding, precision casting, additive manufacturing, or other non?mechanical processing technology, it is diffcult to meet the molding precision required for direct use, and the surface quality and accuracy of the shape need to be improved through a finishing process. In view of the poor reachability of the current tool?based polishing process, a tool?less polishing method using free?abrasive grains for complex spiral grooves is proposed. With this method, by controlling the movement of the workpiece, the design basis and relative motion of the abrasive particles along a helical path remain consistent, resulting in a better polishing profile. A spiral groove of a revolving body is taken as the research object; the influence of the installation method and the position of the parts, as well as the effect of the rotational speed of the abrasive ball on its relative motion along a helical trajectory, are studied, and the polishing cutting process of an abrasive ball is reasonably simplified. A consistent math?ematical model of the trajectory of an abrasive ball relative to the design helix is constructed. The grooved drum parts are verified through a polishing experiment. The spiral groove of the revolving body is modified and polished. Experiments show that the process not only corrects the shape a spiral groove error, but also reduces the surface roughness of a spiral groove. This study provides a theoretical basis for achieving free?abrasive, tool?free polishing.
基金This study was supported by the National Natural Science Foundation of China(No.81573586).
文摘Aiming to ensure the consistency of quality control of Traditional Chinese Medicines(TCMs),a combination method of high-performance liquid chromatography(HPLC),ultraviolet(UV),electrochemical(EC)was developed in this study to comprehensively evaluate the quality of Antiviral Mixture(AM),and Comprehensive Linear Quantification Fingerprint Method(CLQFM)was used to process the data.Quantitative analysis of three active substances in TCM was conducted.A fivewavelength fusion fingerprint(FWFF)was developed,using second-order derivatives of UV spectral data to differentiate sample levels effectively.The combination of HPLC and UV spectrophotometry,along with electrochemical fingerprinting(ECFP),successfully evaluated total active substances.Ultimately,a multidimensional profiling analytical system for TCM was developed.
基金the deanship of Scientific Research at Jouf University for founding this work through research grant no(DSR2020-02-387).https://www.ju.edu.sa/.
文摘This paper presents a new optimization study of the placement and size of a photovoltaic source(PVS)in a distribution grid,based on annual records of meteorological parameters(irradiance,temperature).Based on the recorded data,the production output as well as the daily average power(24-h vector)of the PVS is extracted over the year.When a power vector is available,it can be used as an input when searching for the optimal size of the PVS.This allows to take into account the constraint of the variation of the power generated by this source considering the variation of the power consumed by the electrical loads during the whole day.A multi-objective fitness function has been considered.The latter minimizes the active losses and maximizes the voltage stability index during the day,while considering the constraints of the system,that is,the security,technical,geographical,and meteorological constraints.This problem was solved using the Non-dominated Sorting Genetic Algorithm NSGA-II optimization technique under MATLAB 2021.It was applied to the distribution network of Ghardaïa of 59 nodes.
文摘On the one hand,the separation of thousands of compounds in a complex extract is thrilling,but may be still be separated unsatisfactorily.Hence,the question arises where to stop in high-sophisticated separation science?Which technical effort is economically justifiable in routine?On the other hand,the separation itself does not imply an effect-directed answer to questions such
基金This study was financially supported by the Special Funding Project for Public Welfare Industries(Agriculture)Scientific Research:Research on Mechanized Cultivation Model in Maize Planting Area of State Farm(201503116-04-04)and conducted in the Conservation Tillage Technology Center of the Heilongjiang Provincial Education Department jointly established by both corporations and universities.
文摘The emergence rate and vitality of maize are directly affected by the sowing depth,and the uniformity of this depth is an important performance indicator of a planter,while the effective soil surface height information acquisition is the prerequisite for ensuring the accuracy of sowing depth control.The soil surface height variation acquisition system of a precision corn planter often produces profiling errors when performing active profiling due to interference from ground debris.In this study,a multipoint soil surface height variation information acquisition system was investigated,which consists of a ranging sensor group and a microcontroller unit(MCU)using a data comparison and screening method.The structure and specifications of the ranging sensors were determined according to the soil surface height variation and debris size,and a nonessential profiling control program was developed.Performed tests on the information acquisition system indicated that the measurement accuracy of the system was 3 mm,and when advancing at a speed of 8 km/h,the accuracy of the profiling decision and the system stability were 97.1%and 94.1%,respectively,indicating that the system was capable of nonessential profile control.The designed ranging system could provide a reference for the design of a ground information acquisition system of precision planters with an active profiling mechanism.
基金supported by the CNRS,INSERM,and Aix Marseille University.Proteomics analyses were carried out using the mass spectrometry facility of Marseille Proteomics(marseille‐proteomique.univ‐amu.fr)supported by IBISAthe Cancéropôle PACA,the Provence‐Alpes‐Côte d'Azur Region,the Institut Paoli‐Calmettes,and Fonds Européen de Développement Regional(FEDER)+1 种基金Emma Forest PhD fellowship is supported by the Ministère de l'Enseignement Supérieur et de la RechercheThe authors would like to thank Pierre Santucci(LISM UMR7255 CNRS)for his help with Biorender.
文摘Gram‐negative bacteria are particularly prone to developing antimicrobial resistance(AMR),as evidenced by the WHO's ESKAPEE list of high‐priority pathogens.One strategy that has increased is the use of antibiotic enhancers,which can reempower abandoned or poorly active antibiotics against the resistant strain of interest.In this study,the polyamino‐isoprenyl antibiotic enhancer,NV716,was tested in combination with two families of multi‐target Ser/Cys‐based enzyme inhibitors,the oxadiazolone derivatives(OX)and the Cyclipostins and Cyclophostin analogs(CyC),which are inactive against Gram‐negative ESKAPEE bacteria,to potentiate their antibacterial activity and thus make them active against these bacteria.We demonstrated that NV716 potentiates some OX and CyC compounds by permeabilizing the outer membrane and thus by increasing the inhibitor accumulation,as shown by fluorescence microscopy.By using the click‐chemistry activity‐based protein profiling(ABPP)approach coupled with proteomic analysis,we also confirmed the multi‐target nature of the best OX and CyC inhibitors by identifying their target proteins on a bacterial culture of Enterobacter cloacae.Remarkably,a large set of these identified proteins had already been captured in previous ABPP experiments conducted on Mycobacterium tuberculosis and/or Mycobacterium abscessus culture.Furthermore,we showed that five of the identified target proteins were present in a total lysate of Pseudomonas aeruginosa.Importantly,these latter enzymes are highly conserved among Gram‐negative bacteria,with two of them annotated as essential for bacterial survival.These results provide proof of concept that both OX and CyC,if successfully potentiated,could be used against ESKAPEE Gram‐negative bacteria.
基金supported by USDA cooperative agreement 58-6435-6-055
文摘Plant b-1,3-glucanases are members of the pathogenesis-related protein 2（PR-2） family,which is one of the 17 PR protein families and plays important roles in biotic and abiotic stress responses.One of the differentially expressed proteins（spot 842） identified in a recent proteomic comparison between five pairs of closely related maize（Zea mays L.） lines differing in aflatoxin resistance was further investigated in the present study.Here,the corresponding cDNA was cloned from maize and designated as ZmGns.ZmGns encodes a protein of338 amino acids containing a potential signal peptide.The expression of Zm Gns was detectible in all tissues studied with the highest level in silks.ZmGns was significantly induced by biotic stresses including three bacteria and the fungus Aspergillus flavus.ZmGns was also induced by most abiotic stresses tested and growth hormones including salicylic acid.In vivo,ZmGns showed a significant inhibitory activity against thebacterial pathogen Pseudomonas syringae pv.tomato DC3000 and fungal pathogen Botrytis cinerea when it overexpressed in Arabidopsis.Its high level of expression in the silk tissue and its induced expression by phytohormone treatment,as well as by bacterial and fungal infections,suggest it plays a complex role in maize growth,development,and defense.
