We developed a transient model for actin-based motility.Diffusion of actin monomers was included in the formulation and its influence on the speed of actin-driven cargos was examined in detail.Our results clearly demo...We developed a transient model for actin-based motility.Diffusion of actin monomers was included in the formulation and its influence on the speed of actin-driven cargos was examined in detail.Our results clearly demonstrated how actin polymerization accelerates cargos that are initially stationary,as well as how steady-state is eventually reached.We also found that,due to polymerization and diffusion,actin monomer concentration near the load surface can be significantly lower than that in the rest of the comet tail,suggesting that many previous models may not be very accurate.展开更多
Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors.These tubules are propelled by actin filaments an...Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors.These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors.This is known as actin-based tubule motility(ABTM),in which insect proteins,especially actin-associated proteins participate.To better understand the insect components that play a role in the ABTM,the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus(RGDV)in the leafhopper vector were investigated.We found that gelsolin,an actin-modulating protein,interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells.The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector.Further,the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects.The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation,thus increasing the viruliferous rates of the leafhopper vector.This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules,finally acting to negatively regulate RGDV infection.The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.A020307 and 11072094)the program for New Century Excellent Talents in University(NCET-10-0445)
文摘We developed a transient model for actin-based motility.Diffusion of actin monomers was included in the formulation and its influence on the speed of actin-driven cargos was examined in detail.Our results clearly demonstrated how actin polymerization accelerates cargos that are initially stationary,as well as how steady-state is eventually reached.We also found that,due to polymerization and diffusion,actin monomer concentration near the load surface can be significantly lower than that in the rest of the comet tail,suggesting that many previous models may not be very accurate.
基金supported by the National Natural Science Foundation of China under grant numbers 3173007131772124+2 种基金the Natural Science Foundation of Fujian Province,China under grant number 2017 J06011the National Key R&D Program of China under grant number 2017YFD0200900the Program for New Century Excellent Talents in Fujian Province University under grant number Kla18057A.
文摘Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors.These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors.This is known as actin-based tubule motility(ABTM),in which insect proteins,especially actin-associated proteins participate.To better understand the insect components that play a role in the ABTM,the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus(RGDV)in the leafhopper vector were investigated.We found that gelsolin,an actin-modulating protein,interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells.The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector.Further,the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects.The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation,thus increasing the viruliferous rates of the leafhopper vector.This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules,finally acting to negatively regulate RGDV infection.The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus.
