Background Salmonella enterica serovar Enteritidis(S.Enteritidis)is a global foodborne pathogen that poses a significant threat to human health,with poultry being the primary reservoir host.Therefore,addressing S.Ente...Background Salmonella enterica serovar Enteritidis(S.Enteritidis)is a global foodborne pathogen that poses a significant threat to human health,with poultry being the primary reservoir host.Therefore,addressing S.Enteritidis infections in poultry is crucial to protect human health and the poultry industry.In this study,we investigated the effect of co-housing Arbor Acres(AA)chickens,a commercial breed susceptible to S.Enteritidis,with Tibetan chickens,a local breed resistant to S.Enteritidis infection,on the resistance of the latter to the pathogen.Results Ninety-six 1-day-old Tibetan chickens and 961-day-old AA chickens were divided into a Tibetan chicken housed alone group(n=48),an AA chicken housed alone group(n=48),and a co-housed group(48 birds from each breed for 2 cages).All birds were provided the same diet,and the experimental period lasted 14 d.At d 7,all chickens were infected with S.Enteritidis,and samples were collected at 1-,3-,and 7-day-post-infection.We found that the body weight of AA chickens significantly increased when co-housed with Tibetan chickens at 1-and 3-day-post-infection(P<0.05).In addition,the cecal S.Enteritidis load in AA chickens was significantly reduced at 1-,3-,and 7-day-post-infection(P<0.05).Furthermore,the inflammatory response in AA chickens decreased,as evidenced by the decreased expression of proinflammatory cytokines NOS2,TNF-α,IL-8,IL-1β,and IFN-γin their cecal tonsils(P<0.05).Co-housing with Tibetan chickens significantly increased the height of villi and number of goblet cells(P<0.05),as well as the expression of claudin-1(P<0.05),a tight junction protein,in the jejunum of AA chickens.Further analysis revealed that co-housing altered the gut microbiota composition in AA chickens;specifically,the relative abundances of harmful microbes,such as Intestinimonas,Oscillibacter,Tuzzerella,Anaerotruncus,Paludicola,and Anaerofilum were reduced(P<0.05).Conclusions Our findings indicate that co-housing with Tibetan chickens enhanced the resistance of AA chickens to S.Enteritidis infection without compromising the resistance of Tibetan chickens.This study provides a novel approach for Salmonella control in practical poultry production.展开更多
As one of Shakespeare＇s four great tragedies, King Lear stands as a classic masterpiece firmly in world literary history. The Lear story goes through many retellings, among which Jane Smiley＇s A Thousand Acres prove...As one of Shakespeare＇s four great tragedies, King Lear stands as a classic masterpiece firmly in world literary history. The Lear story goes through many retellings, among which Jane Smiley＇s A Thousand Acres proves to be a successful one. Though exhibited from different perspectives, the two stories have something in common the ironies arising from father-daughter conflicts. In the way of an intertextual comparison, the paper analyzes the ironies from father-daughter conflicts with an aim of digging out deep meaning from behind in a new angle. It f＇mds out that the ironies shared by the two works help to increase the tragic atmosphere and reveal a lot of truths underneath the lines.展开更多
基金supported by the Earmarked fund for China Agriculture Research System of MOF and MARA(Grant No.CARS-41-G01).
文摘Background Salmonella enterica serovar Enteritidis(S.Enteritidis)is a global foodborne pathogen that poses a significant threat to human health,with poultry being the primary reservoir host.Therefore,addressing S.Enteritidis infections in poultry is crucial to protect human health and the poultry industry.In this study,we investigated the effect of co-housing Arbor Acres(AA)chickens,a commercial breed susceptible to S.Enteritidis,with Tibetan chickens,a local breed resistant to S.Enteritidis infection,on the resistance of the latter to the pathogen.Results Ninety-six 1-day-old Tibetan chickens and 961-day-old AA chickens were divided into a Tibetan chicken housed alone group(n=48),an AA chicken housed alone group(n=48),and a co-housed group(48 birds from each breed for 2 cages).All birds were provided the same diet,and the experimental period lasted 14 d.At d 7,all chickens were infected with S.Enteritidis,and samples were collected at 1-,3-,and 7-day-post-infection.We found that the body weight of AA chickens significantly increased when co-housed with Tibetan chickens at 1-and 3-day-post-infection(P<0.05).In addition,the cecal S.Enteritidis load in AA chickens was significantly reduced at 1-,3-,and 7-day-post-infection(P<0.05).Furthermore,the inflammatory response in AA chickens decreased,as evidenced by the decreased expression of proinflammatory cytokines NOS2,TNF-α,IL-8,IL-1β,and IFN-γin their cecal tonsils(P<0.05).Co-housing with Tibetan chickens significantly increased the height of villi and number of goblet cells(P<0.05),as well as the expression of claudin-1(P<0.05),a tight junction protein,in the jejunum of AA chickens.Further analysis revealed that co-housing altered the gut microbiota composition in AA chickens;specifically,the relative abundances of harmful microbes,such as Intestinimonas,Oscillibacter,Tuzzerella,Anaerotruncus,Paludicola,and Anaerofilum were reduced(P<0.05).Conclusions Our findings indicate that co-housing with Tibetan chickens enhanced the resistance of AA chickens to S.Enteritidis infection without compromising the resistance of Tibetan chickens.This study provides a novel approach for Salmonella control in practical poultry production.
文摘As one of Shakespeare＇s four great tragedies, King Lear stands as a classic masterpiece firmly in world literary history. The Lear story goes through many retellings, among which Jane Smiley＇s A Thousand Acres proves to be a successful one. Though exhibited from different perspectives, the two stories have something in common the ironies arising from father-daughter conflicts. In the way of an intertextual comparison, the paper analyzes the ironies from father-daughter conflicts with an aim of digging out deep meaning from behind in a new angle. It f＇mds out that the ironies shared by the two works help to increase the tragic atmosphere and reveal a lot of truths underneath the lines.
