The concise building of the spiroketal core of acortatarin-type alkaloids as potential therapeutic agents in diabetic nephropathy was established in four steps,through a tandem N-alkylation/hemiacetalization between p...The concise building of the spiroketal core of acortatarin-type alkaloids as potential therapeutic agents in diabetic nephropathy was established in four steps,through a tandem N-alkylation/hemiacetalization between pyrrole units and the corresponding halo alcohols generated by convenient halomethylation of chiral lactones from natural aldoses.展开更多
Background Diabetic nephropathy （DN） is the leading cause of end-stage renal disease. Various treatment regimens and combinations of therapies provide only partial renoprotection. Therefore new approaches are needed...Background Diabetic nephropathy （DN） is the leading cause of end-stage renal disease. Various treatment regimens and combinations of therapies provide only partial renoprotection. Therefore new approaches are needed to retard the progression of DN. The aim of the present study was to evaluate the role of a novel spiroalkaloid from Acorus tatarinowii named acortatarin A （AcorA） in inhibiting high glucose-induced extracellular matrix accumulation in mesangial cells （MCs）. Methods The cytotoxity of AcorA on MCs was examined by 3-（4,5-dimethylthiazol-2-yl）-2.5-diphenyltetrazolium bromide （MTT） assay. The expression of fibronectin and collagen IV was examined by real time PCR and western blotting. The expression of p22phox and p47phox was detected by western blot. The interaction between p22phox and p47phox was examined by co-immunoprecipitation. The phosphorylation of p47phox was examined by immunoprecipitation. The phosphorylation of protein kinase C （PKC） α, PKCβ, phospholiase C gamma （PLCγ1）, and the p85 subunit of PI3K was determined by Western blotting. Results AcorA significantly inhibited high glucose-induced activation of NADPH oxidase, a ROS-generating enzyme, by increasing phosphorylation of p47phox and enhancing interaction between p22phox and p47phox. Preincubation of AcorA with MCs inhibited high glucose-induced collagen IV and fibronectin production in a dose-dependent manner. Moreover, AcorA attenuated high glucose enhanced phosphorylation of PKCα, PKCβ, PLCγ1, and the p85 subunit of PI3K. Conclusion AcorA inhibits high glucose-induced extracellular matrix production via blockinq NADPH oxidase activation.展开更多
基金the“Hundred Talents Project”of Chinese Academy of Science and“High-end Science and Technology Talents Program”’of Yunnan Province(2011HA008)。
文摘The concise building of the spiroketal core of acortatarin-type alkaloids as potential therapeutic agents in diabetic nephropathy was established in four steps,through a tandem N-alkylation/hemiacetalization between pyrrole units and the corresponding halo alcohols generated by convenient halomethylation of chiral lactones from natural aldoses.
基金This stucty was supported by the National ＂973＂ Program （No. 2012CB517700） and the National Natural Science Foundation of China （No. U0932002） to Dr. HOU Fan-fan the Knowledge Innovation Program of Chinese Academy of Sciences （No. KSCX2-YW-R-211） and the Project of Natural Compound Library Construction from Chinese Academy of Sciences （No. KSCX2-EW-R-15） to Dr. CHENG Yong-xian.
文摘Background Diabetic nephropathy （DN） is the leading cause of end-stage renal disease. Various treatment regimens and combinations of therapies provide only partial renoprotection. Therefore new approaches are needed to retard the progression of DN. The aim of the present study was to evaluate the role of a novel spiroalkaloid from Acorus tatarinowii named acortatarin A （AcorA） in inhibiting high glucose-induced extracellular matrix accumulation in mesangial cells （MCs）. Methods The cytotoxity of AcorA on MCs was examined by 3-（4,5-dimethylthiazol-2-yl）-2.5-diphenyltetrazolium bromide （MTT） assay. The expression of fibronectin and collagen IV was examined by real time PCR and western blotting. The expression of p22phox and p47phox was detected by western blot. The interaction between p22phox and p47phox was examined by co-immunoprecipitation. The phosphorylation of p47phox was examined by immunoprecipitation. The phosphorylation of protein kinase C （PKC） α, PKCβ, phospholiase C gamma （PLCγ1）, and the p85 subunit of PI3K was determined by Western blotting. Results AcorA significantly inhibited high glucose-induced activation of NADPH oxidase, a ROS-generating enzyme, by increasing phosphorylation of p47phox and enhancing interaction between p22phox and p47phox. Preincubation of AcorA with MCs inhibited high glucose-induced collagen IV and fibronectin production in a dose-dependent manner. Moreover, AcorA attenuated high glucose enhanced phosphorylation of PKCα, PKCβ, PLCγ1, and the p85 subunit of PI3K. Conclusion AcorA inhibits high glucose-induced extracellular matrix production via blockinq NADPH oxidase activation.
