[Objectives]This study was conducted to investigate the antibacterial effect of a self-designed acne-relieving Chinese herbal formula and prepare a kind of acne-removing soap according to the antibacterial effect.[Met...[Objectives]This study was conducted to investigate the antibacterial effect of a self-designed acne-relieving Chinese herbal formula and prepare a kind of acne-removing soap according to the antibacterial effect.[Methods]Chinese herbs Lonicera japonica,Taraxacum mongolicum,Scutellaria baicalensis,Angelica dahurica,Centella asiatica and Aloe vera,were decocted with water,and the antibacterial effects on Staphylococcus aureus,Staphylococcus albus,Candida albicans and Propionibacterium acnei were tested by inhibition zone experiments.The soap base was prepared by saponification of coconut oil,palm oil,hazelnut oil and sodium hydroxide,and then the herbal liquid was added into the soap.[Results]The liquid had bacteriostatic effect on S.aureus,S.albus and P.acnes,but no inhibitory effect on C.albicans.The best bacteriostatic effect on S.aureus and S.albus was achieved at 330 mg/ml,and the best bacteriostatic effect on P.acnes was at 160 mg/ml.[Conclusions]The Chinese herbal formula has antibacterial effect.The handmade soap made from the Chinese herbal formula has the characteristics of smooth surface,moderate hardness,fine texture,rich foam and fresh smell.展开更多
To verify the inhibitory ability of the combination on the biofilm production of Propionibacterium acnes and its ameliorative effect on facial acne problems.The ability of the combination to inhibit the biofilm format...To verify the inhibitory ability of the combination on the biofilm production of Propionibacterium acnes and its ameliorative effect on facial acne problems.The ability of the combination to inhibit the biofilm formation of Propionibacterium acnes was verified by suspension quantification and crystal violet staining,and then 60 effective volunteers were selected for the efficacy measurement of topical acne elimination combinations+bases for 4 consecutive weeks,which were measured by a professional instrument,and the volunteers’facial acne lesion areas and acne lesion colours were evaluated respectively.In vitro tests verified that the combination can inhibit the formation of P.acnes biofilm and will not completely kill P.acnes and will not disrupt the micro-ecological balance of the skin;3%and 5%acne combinations can reduce the biofilm of P.acnes by 61.3%and 61.6%respectively;after 4 weeks of continuous use,the area of acne lesions of the samples with 5%acne combinations decreased by 54.61%and the colour of acne lesions decreased by 54.61%compared with the area of acne lesions before the use of the combination.54.61%,acne lesion colour a^(*)decreased by 13.74%,and the subjects’self-assessment of the mildness and non-irritation of the samples in the test group had a 100%agreement rate.The acne combination can improve acne problems by inhibiting the biofilm formation of Propionibacterium acnes,and has a mild and effective acne elimination effect.展开更多
Cutibacterium acnes were used to induce lipase production,and to establish ex-vivo skin model of inflammatory response and post-inflammatory hyperpigmentation,in purpose of exploring the mechanism of chamomilla recuti...Cutibacterium acnes were used to induce lipase production,and to establish ex-vivo skin model of inflammatory response and post-inflammatory hyperpigmentation,in purpose of exploring the mechanism of chamomilla recutita extract.Clinical study was designed to investigate the effects of chamomilla recutita extract on Chinese volunteers with sequelae of adult acne,by evaluating the severity of acne,post-acne erythema and pigmentation,skin basic physiological conditions.The results showed that chamomilla recutita extract inhibited lipase activity,cellular inflammatory response,and melanin production.In the clinical study,the acnes were relieved 173%,with a 78%lower post-acne erythema index and 202%less hyperpigmentation as compared to placebo,after applying samples containing 1%chamomilla recutita extract for 28 days.Meanwhile,chamomilla recutita extract showed instant oil control effect.The extract significantly reduced sebum secretion by 293%,increased skin moisture content by 102%,and strengthened the skin barrier by 193%after 28 days application,which provided favorable skin physiological basis for the prevention and improvement of acne vulgaris and sequelae.展开更多
BACKGROUND Synovitis,acne,pustulosis,hyperostosis,and osteitis(SAPHO)syndrome,a rare inflammatory disease,was described in 1987 by Chamot et al,who identified its common characteristics and transitional forms through ...BACKGROUND Synovitis,acne,pustulosis,hyperostosis,and osteitis(SAPHO)syndrome,a rare inflammatory disease,was described in 1987 by Chamot et al,who identified its common characteristics and transitional forms through case reports.The inci-dence rate is now 1 in 10000 among Caucasians and 0.00144 in 10000 among Japanese.Pneumothorax occurs when gas enters the pleural cavity,typically due to lung disease or chest trauma,and is characterized as either spontaneous or traumatic.Spontaneous pneumothorax is commonly linked to congenital lung tissue abnormalities or lung conditions,such as ruptured pulmonary bullae,emphysema,and bronchial asthma.Respiratory involvement in SAPHO syn-drome has historically been noted.CASE SUMMARY We present the case of a 44-year-old male patient who had previously been diagnosed with SAPHO syndrome.Following a two-year cessation of methotrexate therapy,he experienced recurrent anterior chest wall and joint pain accompanied by dyspnea.We conducted a comprehensive examination for the patient,which included physical examination,laboratory tests,and imaging studies.The clinical presentation and treatment outcomes suggest that his pneumothorax was related to an episode of SAPHO syndrome.We performed closed thoracic drainage surgery for the patient and treated his symptoms with etoricoxib(60 mg daily).Upon discharge,methotrexate was prescribed again.In subsequent follow-ups,there was no recurrence of joint and respiratory symptoms observed in the patient.CONCLUSION SAPHO syndrome has been proposed as a transitional stage between ankylosing spondylitis and psoriatic arthritis.One study reported a 0.29%incidence of spontaneous pneumothorax in patients with ankylosing spondylitis,higher than in the general population.Additionally,30%of patients with SAPHO syndrome test positive for HLA-B27,an antigen linked to ankylosing spondylitis.Symptomatically,some patients with SAPHO syndrome meet diagnostic criteria for ankylosing spondylitis,with sternoclavicular joint involvement and paravertebral ossific-ations resembling syndesmophytes in ankylosing spondylitis.These features suggest a possible linked between SAPHO syndrome and ankylosing spondylitis.展开更多
Tamanu oil, derived from the nuts of Calophyllum inophyllum, has gained increasing attention for its potential in acne management due to its purported anti-inflammatory and wound-healing properties. This analysis eval...Tamanu oil, derived from the nuts of Calophyllum inophyllum, has gained increasing attention for its potential in acne management due to its purported anti-inflammatory and wound-healing properties. This analysis evaluates the efficacy of tamanu oil in acne treatment with a specific focus on its impact on inflammation and scar reduction. The novelty of this research lies in its comprehensive analysis of tamanu oil’s dual mechanism of action: reducing acne-related inflammation and promoting the healing of acne scars. Clinical trials and laboratory analyses were conducted to assess the oil’s effectiveness in diminishing erythema, swelling, and post-acne scarring compared to conventional treatments. Preliminary findings demonstrate that tamanu oil significantly reduces inflammation and accelerates wound healing, potentially offering a promising adjunct or alternative to standard acne therapies. Future research should aim to optimize formulation and application protocols, long-term effects, and comparative therapeutic efficacy with other anti-inflammatory agents. Tamanu oil offers a novel and effective approach to acne management, with potential advantages that go beyond inflammation reduction to include enhanced scar reduction, making it a subject that warrants further investigation.展开更多
Objective Proprionibacterium acnes(P.acnes)-induced inflammatory responses,proliferation and differentiation of keratinocytes contribute to the progression of acne vulgaris(AV).P.acnes was found to enhance the product...Objective Proprionibacterium acnes(P.acnes)-induced inflammatory responses,proliferation and differentiation of keratinocytes contribute to the progression of acne vulgaris(AV).P.acnes was found to enhance the production of interleukin-8(IL-8)by keratinocytes.This study aimed to investigate the role of IL-8 in P.acnes-induced proliferation and differentiation of keratinocytes and the underlying mechanism.Methods The P.acnes-stimulated HaCaT cell(a human keratinocyte cell line)model was established.Western blotting and immunofluorescence were performed to detect the expression of the IL-8 receptors C-X-C motif chemokine receptor 1(CXCR1)and C-X-C motif chemokine receptor 2(CXCR2)on HaCaT cells.Cell counting kit-8(CCK-8)assay,5-ethynyl-20-deoxyuridine(EdU)assay and Western blotting were performed to examine the effects of IL-8/CXCR2 axis on the proliferation and differentiation of HaCaT cells treated with P.acnes,the IL-8 neutralizing antibody,the CXCR2 antagonist(SB225002),or the CXCR1/CXCR2 antagonist(G31P).Western blotting,nuclear and cytoplasmic separation,CCK-8 assay,and EdU assay were employed to determine the downstream pathway of CXCR2 after P.acnes-stimulated HaCaT cells were treated with the CXCR2 antagonist,the protein kinase B(AKT)antagonist(AZD5363),or the constitutively active forkhead box O1(FOXO1)mutant.Finally,autophagy markers were measured in HaCaT cells following the transfection of the FOXO1 mutant or treatment with the autophagy inhibitor 3-methyladenine(3-MA).Results The expression levels of CXCR1 and CXCR2 were significantly increased on the membrane of HaCaT cells following P.acnes stimulation.The IL-8/CXCR2 axis predominantly promoted the proliferation and differentiation of P.acnes-induced HaCaT cells by activating AKT/FOXO1/autophagy signaling.In brief,IL-8 bound to its receptor CXCR2 on the membrane of keratinocytes to activate the AKT/FOXO1 axis.Subsequently,phosphorylated FOXO1 facilitated autophagy to promote the proliferation and differentiation of P.acnes-induced keratinocytes.Conclusion This study demonstrated the novel autocrine effect of IL-8 on the proliferation and differentiation of P.acnes-induced keratinocytes,suggesting a potential therapeutic target for AV.展开更多
AIM:To investigate ocular surface disorders and tear function changes in patients with acne vulgaris and explore the potential relationship between acne vulgaris and dry eye.METHODS:This cross-sectional study included...AIM:To investigate ocular surface disorders and tear function changes in patients with acne vulgaris and explore the potential relationship between acne vulgaris and dry eye.METHODS:This cross-sectional study included right eyes of 53 patients with acne vulgaris and 54 healthy controls.The participants completed the Ocular Surface Disease Index(OSDI)questionnaire.The following ocular surface-related parameters were measured:tear meniscus height(TMH),noninvasive tear breakup time(NIBUT),Schirmer I test(SIT),lipid layer thickness(LLT)score of the tear film,meibum score,meibomian gland orifice obstruction score,the ratio of meibomian gland loss,conjunctival hyperemia score,and corneal fluorescein staining(CFS)score.RESULTS:The stability of the tear film decreased in acne vulgaris patients.In the acne group,the TMH and NIBUT were lower,whereas the OSDI,meibum score,meibomian gland orifice obstruction score,ratio of meibomian gland loss,and conjunctival hyperemia score were higher compared with controls(P<0.05).There were no significant differences in the CFS score,SIT,or LLT score between the groups(P>0.05).In two dry eye groups,the TMH,NIBUT,and LLT score were lower in the acne with dry eye(acne-DE)group,and the meibum score,meibomian gland orifice obstruction score,ratio of meibomian gland loss and conjunctival hyperemia score in the acne-DE group were higher(P<0.05).There were no significant differences between OSDI,SIT,and CFS score(P>0.05).CONCLUSION:Patients with moderate-to-severe acne vulgaris are more likely to experience dry eye than those without acne vulgaris.Reduced tear film stability and meibomian gland structure dysfunction are more pronounced in patients with moderate-to-severe acne and dry eye.展开更多
Acne vulgaris ranks as the second most prevalent dermatological condition worldwide,and there are still insufficient safe and reliable drugs to treat it.Cryptotanshinone(CTS),a bioactive compound derived from traditio...Acne vulgaris ranks as the second most prevalent dermatological condition worldwide,and there are still insufficient safe and reliable drugs to treat it.Cryptotanshinone(CTS),a bioactive compound derived from traditional Chinese medicine Salvia miltiorrhiza,has shown promise for treating acne vulgaris due to its broad-spectrum antimicrobial and significant anti-inflammatory properties.Nevertheless,its local application is hindered by its low solubility and poor skin permeability.To overcome these challenges,a carrierfree pure drug self-assembled nanosystem is employed,which can specifically modify drug molecules based on the disease type and microenvironment,offering a potential for more effective treatment.We designed and synthesized three distinct structures of cationic CTS-peptide conjugates,creating self-assembled nanoparticles.This study has explored their self-assembly behavior,skin permeation,cellular uptake,and both in vitro and in vivo anti-acne effects.Molecular dynamics simulations revealed these nanoparticles form through intermolecular hydrogen bonding andπ-πstacking interactions.Notably,self-assembled nanoparticles demonstrated enhanced bioavailability with higher skin permeation and cellular uptake rates.Furthermore,the nanoparticles exhibited superior anti-acne effects compared to the parent drug,attributed to heightened antimicrobial activity and significant downregulation of the MAPK/NF-κB pathway,leading to reduced expression of pro-inflammatory factors including TNF-α,IL-1βand IL-8.In summary,the carrier-free self-assembled nanoparticles based on CTS-peptide conjugate effectively address the issue of poor skin bioavailability,offering a promising new approach for acne treatment.展开更多
In this letter,we discuss the topic of necessity of routine laboratory monitoring during isotretinoin treatment for acne.According to Park and colleagues,it is advisable to monitor the levels of triglycerides,alanine ...In this letter,we discuss the topic of necessity of routine laboratory monitoring during isotretinoin treatment for acne.According to Park and colleagues,it is advisable to monitor the levels of triglycerides,alanine aminotransferase,and aspartate aminotransferase every 5 to 6 months.Additionally,the levels of total cholesterol and low-density lipoprotein should be checked within the first two months of treatment.Isotretinoin is a commonly prescribed agent mainly used to treat acne.Despite its high effectiveness,it necessitates regular monitoring of laboratory parameters due to its side effect profile.Currently,there remains a lack of consensus on the appropriate frequency for monitoring these parameters during treatment with isotretinoin.This letter will provide insight into this complex and controversial topic.Based on existing literature,we concluded that the incidence of changes in lipid and liver aminotransferase levels during isotretinoin treatment for acne was low and likely clinically insignificant.For generally healthy people,we recommend testing lipid and liver profiles once at baseline and a second time at the peak dosage.However,frequent testing might still be beneficial in certain populations of patients.展开更多
Glycolipids are lipid compounds,which are a type of amphiphilic molecules containing glycosyl ligands.This experiment studied the efficacy of glycolipids on acne skin care from the aspects of antibacterial,anti-inflam...Glycolipids are lipid compounds,which are a type of amphiphilic molecules containing glycosyl ligands.This experiment studied the efficacy of glycolipids on acne skin care from the aspects of antibacterial,anti-inflammatory,anti-allergic,oil-control,soothing and repair.Research results show that glycolipids have excellent antibacterial properties against P.acnes;when the dosage of glycolipids reaches 10μg/mL,the inhibition rate of glycolipids on lipid synthesis in SZ95 cells can reach 20%;glycolipids can induce LPS induction RAW264.7 cells have the inhibitory effect on the release of inflammatory factors IL-6 and NO;when the glycolipids concentration is 15 mg/mL,the inhibition rate of glycolipids on hyaluronidase reaches 45.8%;when the glycolipids concentration is 25μg/mL,the inhibition rate on calcium ion concentration reaches 45.3%;glycolipids have a significant promoting effect on wound healing.Furthermore,human efficacy evaluation shows that glycolipids products have comprehensive care effects on acne skin.This study will help further promote the application of glycolipids in cosmetic products,especially in skin care products for acne skin.展开更多
Routine pelvic examinations in women prescribed oral contraceptive pills (OCPs) for acne management are scrutinized to assess their impact on dermatological outcomes versus gynecological health. A synthesis of clinica...Routine pelvic examinations in women prescribed oral contraceptive pills (OCPs) for acne management are scrutinized to assess their impact on dermatological outcomes versus gynecological health. A synthesis of clinical trials, patient surveys, and medical guidelines reveals that these routine exams do not significantly enhance the effectiveness of acne treatment with OCPs but are linked to increased patient discomfort and false-positive results. Evidence suggests that routine pelvic examinations do not significantly impact the effectiveness of acne management with OCPs but are associated with increased patient discomfort and higher rates of false-positive results. The analysis indicates that a more personalized approach, focusing on selective pelvic examinations based on individual risk factors and clinical symptoms, may offer a more practical and patient-centered alternative. The review advocates for a reexamination of existing guidelines to better align with a model of individualized care, emphasizing the need for further research to optimize best practices at the intersection of dermatological and gynecological care. Adopting a personalized approach to pelvic examinations could substantially improve patient care by minimizing unnecessary interventions and discomfort while maintaining the efficacy of acne treatment with oral contraceptive pills.展开更多
Background: Post-acne scarring remains a current challenge to prevent and treat despite advancement in acne treatment. There are two types of scars, atrophic and hypertrophic scars, depending on whether there is a net...Background: Post-acne scarring remains a current challenge to prevent and treat despite advancement in acne treatment. There are two types of scars, atrophic and hypertrophic scars, depending on whether there is a net loss or gain of collagen. Objective: The main objectives of this study were to compare the efficacy of using microneedling combined with either plasma gel or amniotic fluid gel in treatment of atrophic post acne scars with clinical and histological evaluation. Methods: This study included Twenty-three cases with atrophic post acne scars. The treated area on the face was divided equally into two halves. Before the procedure, we applied thick layer of local anesthetic cream for approximately 45 to 60 minutes. Microneedling using dermapen combined with irradiated amniotic gel topically was applied to left side of the face and platelet rich plasma gel was applied to the right side. Patients received four sessions with 3 weeks interval between sessions. Clinical examination with histopathological analysis was carried out one month after the last session. Results: There was a significant increase in the improvement percentage of acne scars on right side (dermarpen and platelet rich plasma gel) than left side of face (dermapen and irradiated amniotic gel). Histologically, improvement of character of collagen and elastic fibers was noticed, especially on right side. Meanwhile, a significant increase in epidermal thickness on both sides of face was detected. Conclusion: Dermapen combined with either plasma gel or amniotic gel are promising effective therapeutic modalities for atrophic acne scars. However, combination of platelet rich plasma gel with dermapen has a higher therapeutic efficacy than that of amniotic gel with dermapen.展开更多
Background:Acne vulgaris is one of the most common skin diseases that can significantly impact a considerable proportion of individuals over their lifetime.Objective:This study focuses on the exploration of the applic...Background:Acne vulgaris is one of the most common skin diseases that can significantly impact a considerable proportion of individuals over their lifetime.Objective:This study focuses on the exploration of the application potential of bamboo vinegar in cosmetics.Materials and Methods:The stock solution of bamboo vinegar is subjected to reduced-pressure distillation at different temperatures to obtain refined bamboo vinegar.Then,inhibition of Propionibacterium acnes(P.acnes)by refined bamboo vinegar is observed.Moreover,the refined bamboo vinegar is adsorbed and released with activated bamboo charcoal as the carrier.In all,this study aims to probe into the mechanism of the controlledrelease system of bamboo vinegar.Results:The results shows that the harmful substances(tar)in bamboo vinegar distilled at 70℃decreased by 94.44%,which is a more notable decrease compared with that in the stock solution.The total organic acid content in bamboo vinegar after reduced-pressure distillation is 11.840%,reaching the national standard for refined bamboo vinegar(GB/T 31734–2015).Additionally,the minimum inhibitory concentration of refined bamboo vinegar against P.acnes using the punch method is 7.90 mg/mL.This indicates that refined bamboo vinegar has the potential as a prospective raw material for formulations in anti-acne cosmetic products.Furthermore,the release rate of bamboo charcoal/bamboo vinegar in water for 15 min reaches 70.57%,which then slows down to a plateau.The slow-release behavior is agreed with the Ritger-Peppas model and is beneficial to relieve the irritation of bamboo vinegar to the skin and lengthen its bacteriostatic duration.Conclusion:The foregoing conclusions can serve as the theoretical foundation for the application of bamboo vinegar in anti-acne cosmetics.展开更多
Background:Over 85%of teenagers and young adults worldwide suffer from acne vulgaris(AV),a widespread chronic condition that makes up 15–20%of all skin disease cases.Objective:For AV treatment,15%Azelaic acid gel has...Background:Over 85%of teenagers and young adults worldwide suffer from acne vulgaris(AV),a widespread chronic condition that makes up 15–20%of all skin disease cases.Objective:For AV treatment,15%Azelaic acid gel has long been promoted as a safe and efficient therapeutic agent.Our goal was to investigate how the skin microbiota of individuals with acne vulgaris was affected by a 15%Azelaic acid gel.Materials and methods:A total of 55 volunteers who clearly displayed signs of acne vulgaris were recruited.They used a 15%Azelaic acid gel every day for 28 days,with 3 follow-up appointments on the 1st,14th,and 28th days.The V3–V4 region of the 16S rRNA gene was sequenced to examine the skin microbial samples that were collected.Results:The taxonomic makeup of the microbiome communities in acne-affected vs.acne-free locations differs significantly.The Alpha and Beta diversity of acne-affected areas improved after 28 days of applying 15%Azelaic acid gel.There were slight decreases in values of Cutibacterium and Staphylococci,which were not statistically significant.Lactobacillus increased considerably(p^(*)=0.01)from 0.97%to 1.60%.Propionibacterium and Staphylococci relative numbers after extended usage of 15%Azelaic acid gel were similar to those of skin not affected by acne.Conclusion:15%Azelaic acid gel aided in modifying the skin microbiota within the acne vulgaris group increased the diversity of skin microbiota in the acne-affected area.展开更多
Objective To evaluate the efficacy of fire-needle therapy for acne to provide an objective basis for clinical decisions.Method Pub Med,Chinese Biomedical Medicine disc（CBM）,Chinese National knowledge infrastructure...Objective To evaluate the efficacy of fire-needle therapy for acne to provide an objective basis for clinical decisions.Method Pub Med,Chinese Biomedical Medicine disc（CBM）,Chinese National knowledge infrastructure（CNKI）,and Wanfang database were searched to include eligible randomized controlled trails.Bias risk was assessed and data were extracted.Meta-analysis was performed and as was subgroup analysis.Results Thirty-three RCTs involving 3362 patients were included.Most of them had a high risk or unclear risk of bias regarding allocation concealment,incomplete outcome data and selective reporting.Compared with control groups,meta-analysis revealed that fireneedle therapy had an overall higher total effectiveness rate（RR=1.19,95% CI：1.16–1.22,P0.000 01）.Subgroup analysis showed fire-needle therapy was associated with an increased total effective rate（RR=1.20,95%CI：1.14–1.28,P0.000 01）,when compared against drug therapy.Fire-Needle therapy was associated with an increased total effective rate（RR=1.18,95%CI：1.12–1.24,P0.000 01）,when fire-needle plus other TCM therapy was compared against other therapy.fire-needle therapy was associated with an increased total effective rate（RR=1.18,95%CI：1.13–1.24,P0.000 01）,when fire-needle plus Chinese herb therapy was compared against Chinese herb therapy alone.fireneedle therapy was associated with an increased total effective rate（RR=1.28,95%CI：1.18–1.39,P0.000 01）,when compared fire-needle plus Western drug therapy against western drug therapy alone.Adverse events were not reported in most articles.Conclusion Our study showed that fire-needle appears to be an effective therapy for treating acne,but the evidence is currently insufficient due to the poor quality of the studies.The safety of fire-needle therapy is also uncertain due to the small sample size and the lack of reporting in included articles.Larger sample,higher quality studies are needed.展开更多
Objective To observe clinical effect of fire needling and pricking bloodletting on acne vulgaris. Methods Sixty-seven patients of acne vulgaris were treated by fire needling at pimple, blackhead and pustule, and prick...Objective To observe clinical effect of fire needling and pricking bloodletting on acne vulgaris. Methods Sixty-seven patients of acne vulgaris were treated by fire needling at pimple, blackhead and pustule, and pricking bloodletting at ěrjiān （耳尖 EX 11） and Géshū （膈俞 BL 17） by three-edged needle, twice a week. Clinical effect was observed after 8 times of treatment. Results Among 67 cases, 42 cases were cured, 21 cases were markedly effective, and 4 cases were effective. The cured rate was 62.69%, and the cured and markedly effective rate was 94.03%. Conclusion The fire needling and pricking bloodletting has a significant effect in the treatment of acne vulgaris with high cured rate, worthy of clinical promotion展开更多
OBJECTIVE: To culturally translate the cardiff acne disability index(CADI) into Korean, and to examine its relationship with clinical acne severity,pathological patterns, and general quality of life(Qo L).METHODS: The...OBJECTIVE: To culturally translate the cardiff acne disability index(CADI) into Korean, and to examine its relationship with clinical acne severity,pathological patterns, and general quality of life(Qo L).METHODS: The CADI was culturally and lin-guistically translated into Korean via translation,back-translation, and face validity test process. Two hundred and fifty-four Korean adolescents were asked to complete the Korean version of the CADI(K-CADI), the Phlegm Pattern, the Cold-Heat Pattern, and the Korean version of the General Health Questionnaires. A clinician estimated acne severity for the adolescents, using the Korean Acne Grading System. Finally, reliability and validity of the K-CADI was examined, and the relationships between acne severity, Phlegm, Cold, and Heat patterns, and Qo L level were examined via pathway analysis.RESULTS: The K-CADI had satisfactory internal consistency(α = 0.827). The examination of construct validity indicated that the K-CADI had one factor(explaining 59.6% of the total variance). Pathway analysis showed satisfactory model fit(normal fit index = 0.960 and comparative fit index = 0.983),and acne-related Qo L was determinant to Phlegm,Heat, and Cold patterns(0.13-0.27 of β), and Phlegm and Heat patterns lowered one's Qo L level(0.17-0.34 of β).CONCLUSION: The K-CADI is a valid and reliable instrument. Phlegm and Heat patterns should be managed when treating acne since they have a moderating effect on general Qo L aggravation.展开更多
Acne vulgaris is a chronic dermatologic problem with multiple factors involved in its pathogenesis. Alternative solutions to acne treatment were instigated by antibiotic resistance despite of its extensive use. Purifi...Acne vulgaris is a chronic dermatologic problem with multiple factors involved in its pathogenesis. Alternative solutions to acne treatment were instigated by antibiotic resistance despite of its extensive use. Purified bee venom （PBV） has been proposed as a promising candidate for that purpose. The present study was designed to confirm the antibacterial effect of PBV and access the efficacy of cosmetics containing PBV in subjects with acne vulgaris. METHODS： The skin bacterium Propionibacterium acnes was incubated with PBV at various concentrations and bacterial growth was evaluated using the colony forming unit （CFU） assay. The mechanism of PBV employed in killing P. acnes was examined by scanning electron microscopy （SEM） and transmission electron microscopy （TEM）. In addition, a total of 12 subjects were randomized in a double-blind, controlled trial to receive either cosmetics containing PBV or cosmetics without PBV for two weeks. Evaluations included lesion counts and skin microorganism. RESULTS： PBV exhibited antimicrobial activity in a concentration-dependent manner, reducing the number of P. acnes CFU by approximately 6 logs at a concentration of 0.5 mg. When PBV concentration was higher than 1.0 mg, no P. acnes colonies were spotted on an agar. TEM and SEM of untreated P. acnes illustrated the normal pleomorphic structure, whereas the PBV- treated bacterium lost the integrity of surface architecture. Significant difference （P=0.027） in the grading levels based on numbers of lesion counts for inflammatory and noninflammatory was observed in favour of the PBV group compared with the control group. In terms of average decrement of skin microorganism, subjects receiving cosmetics containing PBV experienced a significant 57.5% decrease of adenosine triphosphate levels, whereas participants receiving cosmetics without PBV experienced a nonsignificant decrease of 4.7%. CONCLUSION： These results show that the in vitro actions of antimicrobial activity of PBV were translated in vivo. Cosmetics containing PBV provided a certain degree of efficacy in terms of lesion counts and skin microorganism concentration compared with cosmetics without PBV in subjects with acne vulgaris. PBV may be a good candidate compound for developing therapeutic drua for the treatment of acne vulaaris.展开更多
Objective:Propionibacterium acnes(P.acnes)plays an important role in the development of acne,an inflammatory skin disease with a high-incidence.In this study,we used high-throughput RNA sequencing(RNA-seq)to reveal th...Objective:Propionibacterium acnes(P.acnes)plays an important role in the development of acne,an inflammatory skin disease with a high-incidence.In this study,we used high-throughput RNA sequencing(RNA-seq)to reveal the anti-inflammatory mechanism of baicalin on P.acnes-induced acne in rabbits.Methods:The Kligman method was used to induce acne in the ears of New Zealand rabbits.The effect of baicalin on the acne model was evaluated by the number of acne lesions and hematoxylin and eosin(H&E)staining of acne tissues.Enzyme-linked immunoabsorbent assay was used to measure the protein expression levels of tumor necrosis factor a(TNFA),interleukin-1 b(IL1B),IL6,and IL8 in the serum of rabbits.RNA-seq was performed to investigate the mechanism of anti-inflammatory activities of baicalin on acne.Immunohistochemical analysis and Western blot were used to validate the expression levels of related proteins in acne tissues.Results:Baicalin treatment significantly reduced the number of acne lesions and lesions of the ear as well as levels of serum inflammatory cytokines.RNA-seq data showed that baicalin treatment globally suppressed inflammation,especially the TNF signaling pathway and Staphylococcus aureus infection pathway,in the rabbit acne model.Conclusion: Baicalin effectively ameliorates P. acnes-induced acne in rabbits by suppressingthe inflammatory response in rabbits.展开更多
基金Supported by National Undergraduate Innovation and Entrepreneurship Training Program of Guangxi University of Chinese Medicine in 2022(202210600026).
文摘[Objectives]This study was conducted to investigate the antibacterial effect of a self-designed acne-relieving Chinese herbal formula and prepare a kind of acne-removing soap according to the antibacterial effect.[Methods]Chinese herbs Lonicera japonica,Taraxacum mongolicum,Scutellaria baicalensis,Angelica dahurica,Centella asiatica and Aloe vera,were decocted with water,and the antibacterial effects on Staphylococcus aureus,Staphylococcus albus,Candida albicans and Propionibacterium acnei were tested by inhibition zone experiments.The soap base was prepared by saponification of coconut oil,palm oil,hazelnut oil and sodium hydroxide,and then the herbal liquid was added into the soap.[Results]The liquid had bacteriostatic effect on S.aureus,S.albus and P.acnes,but no inhibitory effect on C.albicans.The best bacteriostatic effect on S.aureus and S.albus was achieved at 330 mg/ml,and the best bacteriostatic effect on P.acnes was at 160 mg/ml.[Conclusions]The Chinese herbal formula has antibacterial effect.The handmade soap made from the Chinese herbal formula has the characteristics of smooth surface,moderate hardness,fine texture,rich foam and fresh smell.
文摘To verify the inhibitory ability of the combination on the biofilm production of Propionibacterium acnes and its ameliorative effect on facial acne problems.The ability of the combination to inhibit the biofilm formation of Propionibacterium acnes was verified by suspension quantification and crystal violet staining,and then 60 effective volunteers were selected for the efficacy measurement of topical acne elimination combinations+bases for 4 consecutive weeks,which were measured by a professional instrument,and the volunteers’facial acne lesion areas and acne lesion colours were evaluated respectively.In vitro tests verified that the combination can inhibit the formation of P.acnes biofilm and will not completely kill P.acnes and will not disrupt the micro-ecological balance of the skin;3%and 5%acne combinations can reduce the biofilm of P.acnes by 61.3%and 61.6%respectively;after 4 weeks of continuous use,the area of acne lesions of the samples with 5%acne combinations decreased by 54.61%and the colour of acne lesions decreased by 54.61%compared with the area of acne lesions before the use of the combination.54.61%,acne lesion colour a^(*)decreased by 13.74%,and the subjects’self-assessment of the mildness and non-irritation of the samples in the test group had a 100%agreement rate.The acne combination can improve acne problems by inhibiting the biofilm formation of Propionibacterium acnes,and has a mild and effective acne elimination effect.
文摘Cutibacterium acnes were used to induce lipase production,and to establish ex-vivo skin model of inflammatory response and post-inflammatory hyperpigmentation,in purpose of exploring the mechanism of chamomilla recutita extract.Clinical study was designed to investigate the effects of chamomilla recutita extract on Chinese volunteers with sequelae of adult acne,by evaluating the severity of acne,post-acne erythema and pigmentation,skin basic physiological conditions.The results showed that chamomilla recutita extract inhibited lipase activity,cellular inflammatory response,and melanin production.In the clinical study,the acnes were relieved 173%,with a 78%lower post-acne erythema index and 202%less hyperpigmentation as compared to placebo,after applying samples containing 1%chamomilla recutita extract for 28 days.Meanwhile,chamomilla recutita extract showed instant oil control effect.The extract significantly reduced sebum secretion by 293%,increased skin moisture content by 102%,and strengthened the skin barrier by 193%after 28 days application,which provided favorable skin physiological basis for the prevention and improvement of acne vulgaris and sequelae.
基金Supported by The National Natural Science Foundation of China,No.82074246.
文摘BACKGROUND Synovitis,acne,pustulosis,hyperostosis,and osteitis(SAPHO)syndrome,a rare inflammatory disease,was described in 1987 by Chamot et al,who identified its common characteristics and transitional forms through case reports.The inci-dence rate is now 1 in 10000 among Caucasians and 0.00144 in 10000 among Japanese.Pneumothorax occurs when gas enters the pleural cavity,typically due to lung disease or chest trauma,and is characterized as either spontaneous or traumatic.Spontaneous pneumothorax is commonly linked to congenital lung tissue abnormalities or lung conditions,such as ruptured pulmonary bullae,emphysema,and bronchial asthma.Respiratory involvement in SAPHO syn-drome has historically been noted.CASE SUMMARY We present the case of a 44-year-old male patient who had previously been diagnosed with SAPHO syndrome.Following a two-year cessation of methotrexate therapy,he experienced recurrent anterior chest wall and joint pain accompanied by dyspnea.We conducted a comprehensive examination for the patient,which included physical examination,laboratory tests,and imaging studies.The clinical presentation and treatment outcomes suggest that his pneumothorax was related to an episode of SAPHO syndrome.We performed closed thoracic drainage surgery for the patient and treated his symptoms with etoricoxib(60 mg daily).Upon discharge,methotrexate was prescribed again.In subsequent follow-ups,there was no recurrence of joint and respiratory symptoms observed in the patient.CONCLUSION SAPHO syndrome has been proposed as a transitional stage between ankylosing spondylitis and psoriatic arthritis.One study reported a 0.29%incidence of spontaneous pneumothorax in patients with ankylosing spondylitis,higher than in the general population.Additionally,30%of patients with SAPHO syndrome test positive for HLA-B27,an antigen linked to ankylosing spondylitis.Symptomatically,some patients with SAPHO syndrome meet diagnostic criteria for ankylosing spondylitis,with sternoclavicular joint involvement and paravertebral ossific-ations resembling syndesmophytes in ankylosing spondylitis.These features suggest a possible linked between SAPHO syndrome and ankylosing spondylitis.
文摘Tamanu oil, derived from the nuts of Calophyllum inophyllum, has gained increasing attention for its potential in acne management due to its purported anti-inflammatory and wound-healing properties. This analysis evaluates the efficacy of tamanu oil in acne treatment with a specific focus on its impact on inflammation and scar reduction. The novelty of this research lies in its comprehensive analysis of tamanu oil’s dual mechanism of action: reducing acne-related inflammation and promoting the healing of acne scars. Clinical trials and laboratory analyses were conducted to assess the oil’s effectiveness in diminishing erythema, swelling, and post-acne scarring compared to conventional treatments. Preliminary findings demonstrate that tamanu oil significantly reduces inflammation and accelerates wound healing, potentially offering a promising adjunct or alternative to standard acne therapies. Future research should aim to optimize formulation and application protocols, long-term effects, and comparative therapeutic efficacy with other anti-inflammatory agents. Tamanu oil offers a novel and effective approach to acne management, with potential advantages that go beyond inflammation reduction to include enhanced scar reduction, making it a subject that warrants further investigation.
基金supported by the National Natural Science Foundation of China(No.82103756).
文摘Objective Proprionibacterium acnes(P.acnes)-induced inflammatory responses,proliferation and differentiation of keratinocytes contribute to the progression of acne vulgaris(AV).P.acnes was found to enhance the production of interleukin-8(IL-8)by keratinocytes.This study aimed to investigate the role of IL-8 in P.acnes-induced proliferation and differentiation of keratinocytes and the underlying mechanism.Methods The P.acnes-stimulated HaCaT cell(a human keratinocyte cell line)model was established.Western blotting and immunofluorescence were performed to detect the expression of the IL-8 receptors C-X-C motif chemokine receptor 1(CXCR1)and C-X-C motif chemokine receptor 2(CXCR2)on HaCaT cells.Cell counting kit-8(CCK-8)assay,5-ethynyl-20-deoxyuridine(EdU)assay and Western blotting were performed to examine the effects of IL-8/CXCR2 axis on the proliferation and differentiation of HaCaT cells treated with P.acnes,the IL-8 neutralizing antibody,the CXCR2 antagonist(SB225002),or the CXCR1/CXCR2 antagonist(G31P).Western blotting,nuclear and cytoplasmic separation,CCK-8 assay,and EdU assay were employed to determine the downstream pathway of CXCR2 after P.acnes-stimulated HaCaT cells were treated with the CXCR2 antagonist,the protein kinase B(AKT)antagonist(AZD5363),or the constitutively active forkhead box O1(FOXO1)mutant.Finally,autophagy markers were measured in HaCaT cells following the transfection of the FOXO1 mutant or treatment with the autophagy inhibitor 3-methyladenine(3-MA).Results The expression levels of CXCR1 and CXCR2 were significantly increased on the membrane of HaCaT cells following P.acnes stimulation.The IL-8/CXCR2 axis predominantly promoted the proliferation and differentiation of P.acnes-induced HaCaT cells by activating AKT/FOXO1/autophagy signaling.In brief,IL-8 bound to its receptor CXCR2 on the membrane of keratinocytes to activate the AKT/FOXO1 axis.Subsequently,phosphorylated FOXO1 facilitated autophagy to promote the proliferation and differentiation of P.acnes-induced keratinocytes.Conclusion This study demonstrated the novel autocrine effect of IL-8 on the proliferation and differentiation of P.acnes-induced keratinocytes,suggesting a potential therapeutic target for AV.
基金Supported by the National Natural Science Foundation of China(No.81260145No.82060175)Priority Union Foundation of Yunnan Provincial Science and Technology Department and Kunming Medical University(No.202001AY070001-050).
文摘AIM:To investigate ocular surface disorders and tear function changes in patients with acne vulgaris and explore the potential relationship between acne vulgaris and dry eye.METHODS:This cross-sectional study included right eyes of 53 patients with acne vulgaris and 54 healthy controls.The participants completed the Ocular Surface Disease Index(OSDI)questionnaire.The following ocular surface-related parameters were measured:tear meniscus height(TMH),noninvasive tear breakup time(NIBUT),Schirmer I test(SIT),lipid layer thickness(LLT)score of the tear film,meibum score,meibomian gland orifice obstruction score,the ratio of meibomian gland loss,conjunctival hyperemia score,and corneal fluorescein staining(CFS)score.RESULTS:The stability of the tear film decreased in acne vulgaris patients.In the acne group,the TMH and NIBUT were lower,whereas the OSDI,meibum score,meibomian gland orifice obstruction score,ratio of meibomian gland loss,and conjunctival hyperemia score were higher compared with controls(P<0.05).There were no significant differences in the CFS score,SIT,or LLT score between the groups(P>0.05).In two dry eye groups,the TMH,NIBUT,and LLT score were lower in the acne with dry eye(acne-DE)group,and the meibum score,meibomian gland orifice obstruction score,ratio of meibomian gland loss and conjunctival hyperemia score in the acne-DE group were higher(P<0.05).There were no significant differences between OSDI,SIT,and CFS score(P>0.05).CONCLUSION:Patients with moderate-to-severe acne vulgaris are more likely to experience dry eye than those without acne vulgaris.Reduced tear film stability and meibomian gland structure dysfunction are more pronounced in patients with moderate-to-severe acne and dry eye.
文摘Acne vulgaris ranks as the second most prevalent dermatological condition worldwide,and there are still insufficient safe and reliable drugs to treat it.Cryptotanshinone(CTS),a bioactive compound derived from traditional Chinese medicine Salvia miltiorrhiza,has shown promise for treating acne vulgaris due to its broad-spectrum antimicrobial and significant anti-inflammatory properties.Nevertheless,its local application is hindered by its low solubility and poor skin permeability.To overcome these challenges,a carrierfree pure drug self-assembled nanosystem is employed,which can specifically modify drug molecules based on the disease type and microenvironment,offering a potential for more effective treatment.We designed and synthesized three distinct structures of cationic CTS-peptide conjugates,creating self-assembled nanoparticles.This study has explored their self-assembly behavior,skin permeation,cellular uptake,and both in vitro and in vivo anti-acne effects.Molecular dynamics simulations revealed these nanoparticles form through intermolecular hydrogen bonding andπ-πstacking interactions.Notably,self-assembled nanoparticles demonstrated enhanced bioavailability with higher skin permeation and cellular uptake rates.Furthermore,the nanoparticles exhibited superior anti-acne effects compared to the parent drug,attributed to heightened antimicrobial activity and significant downregulation of the MAPK/NF-κB pathway,leading to reduced expression of pro-inflammatory factors including TNF-α,IL-1βand IL-8.In summary,the carrier-free self-assembled nanoparticles based on CTS-peptide conjugate effectively address the issue of poor skin bioavailability,offering a promising new approach for acne treatment.
文摘In this letter,we discuss the topic of necessity of routine laboratory monitoring during isotretinoin treatment for acne.According to Park and colleagues,it is advisable to monitor the levels of triglycerides,alanine aminotransferase,and aspartate aminotransferase every 5 to 6 months.Additionally,the levels of total cholesterol and low-density lipoprotein should be checked within the first two months of treatment.Isotretinoin is a commonly prescribed agent mainly used to treat acne.Despite its high effectiveness,it necessitates regular monitoring of laboratory parameters due to its side effect profile.Currently,there remains a lack of consensus on the appropriate frequency for monitoring these parameters during treatment with isotretinoin.This letter will provide insight into this complex and controversial topic.Based on existing literature,we concluded that the incidence of changes in lipid and liver aminotransferase levels during isotretinoin treatment for acne was low and likely clinically insignificant.For generally healthy people,we recommend testing lipid and liver profiles once at baseline and a second time at the peak dosage.However,frequent testing might still be beneficial in certain populations of patients.
文摘Glycolipids are lipid compounds,which are a type of amphiphilic molecules containing glycosyl ligands.This experiment studied the efficacy of glycolipids on acne skin care from the aspects of antibacterial,anti-inflammatory,anti-allergic,oil-control,soothing and repair.Research results show that glycolipids have excellent antibacterial properties against P.acnes;when the dosage of glycolipids reaches 10μg/mL,the inhibition rate of glycolipids on lipid synthesis in SZ95 cells can reach 20%;glycolipids can induce LPS induction RAW264.7 cells have the inhibitory effect on the release of inflammatory factors IL-6 and NO;when the glycolipids concentration is 15 mg/mL,the inhibition rate of glycolipids on hyaluronidase reaches 45.8%;when the glycolipids concentration is 25μg/mL,the inhibition rate on calcium ion concentration reaches 45.3%;glycolipids have a significant promoting effect on wound healing.Furthermore,human efficacy evaluation shows that glycolipids products have comprehensive care effects on acne skin.This study will help further promote the application of glycolipids in cosmetic products,especially in skin care products for acne skin.
文摘Routine pelvic examinations in women prescribed oral contraceptive pills (OCPs) for acne management are scrutinized to assess their impact on dermatological outcomes versus gynecological health. A synthesis of clinical trials, patient surveys, and medical guidelines reveals that these routine exams do not significantly enhance the effectiveness of acne treatment with OCPs but are linked to increased patient discomfort and false-positive results. Evidence suggests that routine pelvic examinations do not significantly impact the effectiveness of acne management with OCPs but are associated with increased patient discomfort and higher rates of false-positive results. The analysis indicates that a more personalized approach, focusing on selective pelvic examinations based on individual risk factors and clinical symptoms, may offer a more practical and patient-centered alternative. The review advocates for a reexamination of existing guidelines to better align with a model of individualized care, emphasizing the need for further research to optimize best practices at the intersection of dermatological and gynecological care. Adopting a personalized approach to pelvic examinations could substantially improve patient care by minimizing unnecessary interventions and discomfort while maintaining the efficacy of acne treatment with oral contraceptive pills.
文摘Background: Post-acne scarring remains a current challenge to prevent and treat despite advancement in acne treatment. There are two types of scars, atrophic and hypertrophic scars, depending on whether there is a net loss or gain of collagen. Objective: The main objectives of this study were to compare the efficacy of using microneedling combined with either plasma gel or amniotic fluid gel in treatment of atrophic post acne scars with clinical and histological evaluation. Methods: This study included Twenty-three cases with atrophic post acne scars. The treated area on the face was divided equally into two halves. Before the procedure, we applied thick layer of local anesthetic cream for approximately 45 to 60 minutes. Microneedling using dermapen combined with irradiated amniotic gel topically was applied to left side of the face and platelet rich plasma gel was applied to the right side. Patients received four sessions with 3 weeks interval between sessions. Clinical examination with histopathological analysis was carried out one month after the last session. Results: There was a significant increase in the improvement percentage of acne scars on right side (dermarpen and platelet rich plasma gel) than left side of face (dermapen and irradiated amniotic gel). Histologically, improvement of character of collagen and elastic fibers was noticed, especially on right side. Meanwhile, a significant increase in epidermal thickness on both sides of face was detected. Conclusion: Dermapen combined with either plasma gel or amniotic gel are promising effective therapeutic modalities for atrophic acne scars. However, combination of platelet rich plasma gel with dermapen has a higher therapeutic efficacy than that of amniotic gel with dermapen.
文摘Background:Acne vulgaris is one of the most common skin diseases that can significantly impact a considerable proportion of individuals over their lifetime.Objective:This study focuses on the exploration of the application potential of bamboo vinegar in cosmetics.Materials and Methods:The stock solution of bamboo vinegar is subjected to reduced-pressure distillation at different temperatures to obtain refined bamboo vinegar.Then,inhibition of Propionibacterium acnes(P.acnes)by refined bamboo vinegar is observed.Moreover,the refined bamboo vinegar is adsorbed and released with activated bamboo charcoal as the carrier.In all,this study aims to probe into the mechanism of the controlledrelease system of bamboo vinegar.Results:The results shows that the harmful substances(tar)in bamboo vinegar distilled at 70℃decreased by 94.44%,which is a more notable decrease compared with that in the stock solution.The total organic acid content in bamboo vinegar after reduced-pressure distillation is 11.840%,reaching the national standard for refined bamboo vinegar(GB/T 31734–2015).Additionally,the minimum inhibitory concentration of refined bamboo vinegar against P.acnes using the punch method is 7.90 mg/mL.This indicates that refined bamboo vinegar has the potential as a prospective raw material for formulations in anti-acne cosmetic products.Furthermore,the release rate of bamboo charcoal/bamboo vinegar in water for 15 min reaches 70.57%,which then slows down to a plateau.The slow-release behavior is agreed with the Ritger-Peppas model and is beneficial to relieve the irritation of bamboo vinegar to the skin and lengthen its bacteriostatic duration.Conclusion:The foregoing conclusions can serve as the theoretical foundation for the application of bamboo vinegar in anti-acne cosmetics.
文摘Background:Over 85%of teenagers and young adults worldwide suffer from acne vulgaris(AV),a widespread chronic condition that makes up 15–20%of all skin disease cases.Objective:For AV treatment,15%Azelaic acid gel has long been promoted as a safe and efficient therapeutic agent.Our goal was to investigate how the skin microbiota of individuals with acne vulgaris was affected by a 15%Azelaic acid gel.Materials and methods:A total of 55 volunteers who clearly displayed signs of acne vulgaris were recruited.They used a 15%Azelaic acid gel every day for 28 days,with 3 follow-up appointments on the 1st,14th,and 28th days.The V3–V4 region of the 16S rRNA gene was sequenced to examine the skin microbial samples that were collected.Results:The taxonomic makeup of the microbiome communities in acne-affected vs.acne-free locations differs significantly.The Alpha and Beta diversity of acne-affected areas improved after 28 days of applying 15%Azelaic acid gel.There were slight decreases in values of Cutibacterium and Staphylococci,which were not statistically significant.Lactobacillus increased considerably(p^(*)=0.01)from 0.97%to 1.60%.Propionibacterium and Staphylococci relative numbers after extended usage of 15%Azelaic acid gel were similar to those of skin not affected by acne.Conclusion:15%Azelaic acid gel aided in modifying the skin microbiota within the acne vulgaris group increased the diversity of skin microbiota in the acne-affected area.
基金Supported by National Basic Research Program of China(973 program):2012CB518504Guangdong Administration of TCM Research Foundation:20151233
文摘Objective To evaluate the efficacy of fire-needle therapy for acne to provide an objective basis for clinical decisions.Method Pub Med,Chinese Biomedical Medicine disc（CBM）,Chinese National knowledge infrastructure（CNKI）,and Wanfang database were searched to include eligible randomized controlled trails.Bias risk was assessed and data were extracted.Meta-analysis was performed and as was subgroup analysis.Results Thirty-three RCTs involving 3362 patients were included.Most of them had a high risk or unclear risk of bias regarding allocation concealment,incomplete outcome data and selective reporting.Compared with control groups,meta-analysis revealed that fireneedle therapy had an overall higher total effectiveness rate（RR=1.19,95% CI：1.16–1.22,P0.000 01）.Subgroup analysis showed fire-needle therapy was associated with an increased total effective rate（RR=1.20,95%CI：1.14–1.28,P0.000 01）,when compared against drug therapy.Fire-Needle therapy was associated with an increased total effective rate（RR=1.18,95%CI：1.12–1.24,P0.000 01）,when fire-needle plus other TCM therapy was compared against other therapy.fire-needle therapy was associated with an increased total effective rate（RR=1.18,95%CI：1.13–1.24,P0.000 01）,when fire-needle plus Chinese herb therapy was compared against Chinese herb therapy alone.fireneedle therapy was associated with an increased total effective rate（RR=1.28,95%CI：1.18–1.39,P0.000 01）,when compared fire-needle plus Western drug therapy against western drug therapy alone.Adverse events were not reported in most articles.Conclusion Our study showed that fire-needle appears to be an effective therapy for treating acne,but the evidence is currently insufficient due to the poor quality of the studies.The safety of fire-needle therapy is also uncertain due to the small sample size and the lack of reporting in included articles.Larger sample,higher quality studies are needed.
文摘Objective To observe clinical effect of fire needling and pricking bloodletting on acne vulgaris. Methods Sixty-seven patients of acne vulgaris were treated by fire needling at pimple, blackhead and pustule, and pricking bloodletting at ěrjiān （耳尖 EX 11） and Géshū （膈俞 BL 17） by three-edged needle, twice a week. Clinical effect was observed after 8 times of treatment. Results Among 67 cases, 42 cases were cured, 21 cases were markedly effective, and 4 cases were effective. The cured rate was 62.69%, and the cured and markedly effective rate was 94.03%. Conclusion The fire needling and pricking bloodletting has a significant effect in the treatment of acne vulgaris with high cured rate, worthy of clinical promotion
文摘OBJECTIVE: To culturally translate the cardiff acne disability index(CADI) into Korean, and to examine its relationship with clinical acne severity,pathological patterns, and general quality of life(Qo L).METHODS: The CADI was culturally and lin-guistically translated into Korean via translation,back-translation, and face validity test process. Two hundred and fifty-four Korean adolescents were asked to complete the Korean version of the CADI(K-CADI), the Phlegm Pattern, the Cold-Heat Pattern, and the Korean version of the General Health Questionnaires. A clinician estimated acne severity for the adolescents, using the Korean Acne Grading System. Finally, reliability and validity of the K-CADI was examined, and the relationships between acne severity, Phlegm, Cold, and Heat patterns, and Qo L level were examined via pathway analysis.RESULTS: The K-CADI had satisfactory internal consistency(α = 0.827). The examination of construct validity indicated that the K-CADI had one factor(explaining 59.6% of the total variance). Pathway analysis showed satisfactory model fit(normal fit index = 0.960 and comparative fit index = 0.983),and acne-related Qo L was determinant to Phlegm,Heat, and Cold patterns(0.13-0.27 of β), and Phlegm and Heat patterns lowered one's Qo L level(0.17-0.34 of β).CONCLUSION: The K-CADI is a valid and reliable instrument. Phlegm and Heat patterns should be managed when treating acne since they have a moderating effect on general Qo L aggravation.
基金supported by a grant from BioGreen21 Program, Rural Development Administration (Code#:PJ009519), Republic of Korea
文摘Acne vulgaris is a chronic dermatologic problem with multiple factors involved in its pathogenesis. Alternative solutions to acne treatment were instigated by antibiotic resistance despite of its extensive use. Purified bee venom （PBV） has been proposed as a promising candidate for that purpose. The present study was designed to confirm the antibacterial effect of PBV and access the efficacy of cosmetics containing PBV in subjects with acne vulgaris. METHODS： The skin bacterium Propionibacterium acnes was incubated with PBV at various concentrations and bacterial growth was evaluated using the colony forming unit （CFU） assay. The mechanism of PBV employed in killing P. acnes was examined by scanning electron microscopy （SEM） and transmission electron microscopy （TEM）. In addition, a total of 12 subjects were randomized in a double-blind, controlled trial to receive either cosmetics containing PBV or cosmetics without PBV for two weeks. Evaluations included lesion counts and skin microorganism. RESULTS： PBV exhibited antimicrobial activity in a concentration-dependent manner, reducing the number of P. acnes CFU by approximately 6 logs at a concentration of 0.5 mg. When PBV concentration was higher than 1.0 mg, no P. acnes colonies were spotted on an agar. TEM and SEM of untreated P. acnes illustrated the normal pleomorphic structure, whereas the PBV- treated bacterium lost the integrity of surface architecture. Significant difference （P=0.027） in the grading levels based on numbers of lesion counts for inflammatory and noninflammatory was observed in favour of the PBV group compared with the control group. In terms of average decrement of skin microorganism, subjects receiving cosmetics containing PBV experienced a significant 57.5% decrease of adenosine triphosphate levels, whereas participants receiving cosmetics without PBV experienced a nonsignificant decrease of 4.7%. CONCLUSION： These results show that the in vitro actions of antimicrobial activity of PBV were translated in vivo. Cosmetics containing PBV provided a certain degree of efficacy in terms of lesion counts and skin microorganism concentration compared with cosmetics without PBV in subjects with acne vulgaris. PBV may be a good candidate compound for developing therapeutic drua for the treatment of acne vulaaris.
基金This work was supported by the National Natural Science Foundation of China(81430099)International S&T Cooperation Program of China(2014DFA32950).
文摘Objective:Propionibacterium acnes(P.acnes)plays an important role in the development of acne,an inflammatory skin disease with a high-incidence.In this study,we used high-throughput RNA sequencing(RNA-seq)to reveal the anti-inflammatory mechanism of baicalin on P.acnes-induced acne in rabbits.Methods:The Kligman method was used to induce acne in the ears of New Zealand rabbits.The effect of baicalin on the acne model was evaluated by the number of acne lesions and hematoxylin and eosin(H&E)staining of acne tissues.Enzyme-linked immunoabsorbent assay was used to measure the protein expression levels of tumor necrosis factor a(TNFA),interleukin-1 b(IL1B),IL6,and IL8 in the serum of rabbits.RNA-seq was performed to investigate the mechanism of anti-inflammatory activities of baicalin on acne.Immunohistochemical analysis and Western blot were used to validate the expression levels of related proteins in acne tissues.Results:Baicalin treatment significantly reduced the number of acne lesions and lesions of the ear as well as levels of serum inflammatory cytokines.RNA-seq data showed that baicalin treatment globally suppressed inflammation,especially the TNF signaling pathway and Staphylococcus aureus infection pathway,in the rabbit acne model.Conclusion: Baicalin effectively ameliorates P. acnes-induced acne in rabbits by suppressingthe inflammatory response in rabbits.
