Trehalose production via a one-step enzymatic route using trehalose synthase(TreS)holds significant promise for industrial-scale applications due to its simplicity and utilization of low-cost substrates.However,the de...Trehalose production via a one-step enzymatic route using trehalose synthase(TreS)holds significant promise for industrial-scale applications due to its simplicity and utilization of low-cost substrates.However,the development of a robust whole-cell biocatalyst expressing TreS remains crucial for enabling practical and economically viable production.In this study,a high-sugar tolerant strain of S.cerevisiae was screened and employed as a host cell for the cell surface display of TreS from Acidiplasma aeolicum.The resultant strain,S.cerevisiae I3A,exhibited remarkable surface displayed TreS activity of 3358 U/g CDW and achieved approximately 64%trehalose yield(10.8 g/L/h productivity)from maltose.Interestingly,no glucose by-product was observed during trehalose production.The S.cerevisiae I3A cells exhibited reusability for up to 12 cycles leading to potential cost reduction of trehalose products.Therefore,our study demonstrated the development of a high-sugar tolerant S.cerevisiae strain expressing TreS on its surface as a whole-cell biocatalyst for efficient and economical trehalose production with potential applications in the food and pharmaceutical industries.展开更多
基金financially supported by the National Science,Research and Innovation Fund,Thailand Science Research and Innovation(TSRI)[Grant No.:FFB670076/0337]the National Science and Technology Development Agency(NSTDA)[grant number P-20-51068]the NSRF via the Program Management Unit for Human Resources&Institutional Development,Research and Innovation[grant number B13F670055].
文摘Trehalose production via a one-step enzymatic route using trehalose synthase(TreS)holds significant promise for industrial-scale applications due to its simplicity and utilization of low-cost substrates.However,the development of a robust whole-cell biocatalyst expressing TreS remains crucial for enabling practical and economically viable production.In this study,a high-sugar tolerant strain of S.cerevisiae was screened and employed as a host cell for the cell surface display of TreS from Acidiplasma aeolicum.The resultant strain,S.cerevisiae I3A,exhibited remarkable surface displayed TreS activity of 3358 U/g CDW and achieved approximately 64%trehalose yield(10.8 g/L/h productivity)from maltose.Interestingly,no glucose by-product was observed during trehalose production.The S.cerevisiae I3A cells exhibited reusability for up to 12 cycles leading to potential cost reduction of trehalose products.Therefore,our study demonstrated the development of a high-sugar tolerant S.cerevisiae strain expressing TreS on its surface as a whole-cell biocatalyst for efficient and economical trehalose production with potential applications in the food and pharmaceutical industries.
