Using cDNA representational difference analysis (cDNA RDA) method, we have successfully isolated a gene fragment whose expression was specifically induced by external GAa application. Screening a G2 pea cDNA library u...Using cDNA representational difference analysis (cDNA RDA) method, we have successfully isolated a gene fragment whose expression was specifically induced by external GAa application. Screening a G2 pea cDNA library using this fragment as a probe, we obtained a 2036 bp full-length cDNA. It contains a 1746 bp open reading frame and encodes a protein of 581 amino acids with a theoretical molecular weight of 64 ku. It shares high-level sequenceidentity with AAIR genes from other plant species. This cDNA was cloned into expression vector and recombinant E. coli DH5α cells with remarkable AAIR enzyme activity were obtained.展开更多
Two novel compounds 1-（4-fluorobenzyl）-4-chloro-（Z）-3-benzoylhydrazono-2-indolinone（1） and 1-（4-methoxybenzyl）-（Z）-3-benzoylhydrazono-2-indolinone（2） were synthesized and their crystal structures were dete...Two novel compounds 1-（4-fluorobenzyl）-4-chloro-（Z）-3-benzoylhydrazono-2-indolinone（1） and 1-（4-methoxybenzyl）-（Z）-3-benzoylhydrazono-2-indolinone（2） were synthesized and their crystal structures were determined by single-crystal X-ray diffraction.Compound 1（C22H15ClFN3O2） crystallized in the triclinic system,space group P1·with a=0.94198（19） nm,b=1.4339（3） nm,c=1.5018（3） nm,α=101.58（3）°,β=102.96（3）°,γ=102.73°,V=1.8602（6） nm3,Mr=407.82,Dc=1.456 g/cm3,μ=0.240 mm-1,F（000）=840,Z=4,R1=0.0442 and wR2=0.1064.Compound 2（C23H19N3O3） crystallized in the triclinic system,space group P1·with a=1.0022（2） nm,b=1.0192（2） nm,c=1.0461（2） nm,α=99.86（3）°,β=117.30（3）°,γ=94.13（3）°,V=0.9215（3） nm3,Mr=385.41,Dc=1.389 g/cm3,μ=0.094 mm-1,F（000）=404,Z=2,R1=0.0403 and wR2=0.1142.The preliminary herbicidal activities of the two compounds were also evaluated.展开更多
基金This work was supported by the National Science Foundation for Distinguished Young Scholars (Grant No. 39725002) and the National "863" High-Tech Project (Grant No. 101-01-01-02).
文摘Using cDNA representational difference analysis (cDNA RDA) method, we have successfully isolated a gene fragment whose expression was specifically induced by external GAa application. Screening a G2 pea cDNA library using this fragment as a probe, we obtained a 2036 bp full-length cDNA. It contains a 1746 bp open reading frame and encodes a protein of 581 amino acids with a theoretical molecular weight of 64 ku. It shares high-level sequenceidentity with AAIR genes from other plant species. This cDNA was cloned into expression vector and recombinant E. coli DH5α cells with remarkable AAIR enzyme activity were obtained.
基金Supported by the China-Australia Linkage Grant form of National Natural Science Foundation,China(No.20911120022)the National Key Technology Research and Development Program of China(No.2011BAE06B05-3)
文摘Two novel compounds 1-（4-fluorobenzyl）-4-chloro-（Z）-3-benzoylhydrazono-2-indolinone（1） and 1-（4-methoxybenzyl）-（Z）-3-benzoylhydrazono-2-indolinone（2） were synthesized and their crystal structures were determined by single-crystal X-ray diffraction.Compound 1（C22H15ClFN3O2） crystallized in the triclinic system,space group P1·with a=0.94198（19） nm,b=1.4339（3） nm,c=1.5018（3） nm,α=101.58（3）°,β=102.96（3）°,γ=102.73°,V=1.8602（6） nm3,Mr=407.82,Dc=1.456 g/cm3,μ=0.240 mm-1,F（000）=840,Z=4,R1=0.0442 and wR2=0.1064.Compound 2（C23H19N3O3） crystallized in the triclinic system,space group P1·with a=1.0022（2） nm,b=1.0192（2） nm,c=1.0461（2） nm,α=99.86（3）°,β=117.30（3）°,γ=94.13（3）°,V=0.9215（3） nm3,Mr=385.41,Dc=1.389 g/cm3,μ=0.094 mm-1,F（000）=404,Z=2,R1=0.0403 and wR2=0.1142.The preliminary herbicidal activities of the two compounds were also evaluated.
