[Objective] Cloning of the AtrMYB transcription factor gene from Acer truncatum was conducted to further explore the red leaf development mechanism and breed cultivars of colored-leaf maple. [Method] The Acer truncat...[Objective] Cloning of the AtrMYB transcription factor gene from Acer truncatum was conducted to further explore the red leaf development mechanism and breed cultivars of colored-leaf maple. [Method] The Acer truncatum ‘Luhong No.1＇ cultivar was used as the material for cloning the MYB gene by mean of RTPCR and RACE-PCR. [Results] Sequence analysis showed that the fragment contained a full coding region of 831 bp encoding 276 amino acid residues with a molecular weight of 32.17 kD and a molecular formula C_（1430）H_（14052）N_（2247）O_（406）S_（14）. The gene was named as AtrMYB with a Gen Bank accession number of 1825712. This coded protein had apI of 9.44. The results showed that the AtrMYB exhibited typical features of the R2R3-MYB domain. The AtrMYB was highly homologous with the MYB of other species at nucleotide and amino acid levels. The AtrMYB had no signal peptide, but a nuclear localization signal. The phylogenetic tree showed that the AtrMYB was at the same clade as the MYB from Citrus sinensis. [Conclusion] The AtrMYB was cloned from Acer truncatum ‘Luhong No.1＇ cultivar. These results have provided a foundation for further purification and identification of target protein and function study of the AtrMYB.展开更多
[Objective] This study aimed to clone and analyze the sequence of CHS gene from Acer truncatum leaves. [Method] Using A. truncatum cultivars No.1-6 as experimental materials, total RNA was extracted from A. truncatum ...[Objective] This study aimed to clone and analyze the sequence of CHS gene from Acer truncatum leaves. [Method] Using A. truncatum cultivars No.1-6 as experimental materials, total RNA was extracted from A. truncatum leaves with the modified CTAB method. CHS gene sequences were downloaded from the NCBI and aligned by BLAST. Degenerate primers were designed by DNAMAN and Primer- premier5 to amplify the target band. CHS gene fragment was amplified by RT-PCR and ligated to pMD18-T vector. The identified positive colonies were sequenced. [Result] A 1 365 bp fragment was amplified. Sequence analysis suggested that the obtained fragment encoded 365 amino acids and shared above 90% homology to nucleotide sequence of CHS gene from A. palmatum and A. [Conclusion] In this study, CHS gene was successfully cloned from A. truncatum for the first time, which laid the foundation for efficient utilization of CHS gene.展开更多
Nervonic acid is the world’s first and only potent substance that can repair damaged nerve fibers and promote nerve cell regeneration with high nutritional value.The wide variety of fatty acids in plant oils and fats...Nervonic acid is the world’s first and only potent substance that can repair damaged nerve fibers and promote nerve cell regeneration with high nutritional value.The wide variety of fatty acids in plant oils and fats with similar structures makes the large-scale separation and purification of high-purity nervonic acid very difficult.A new combined process of molecular distillation,urea inclusion and solvent crystallization was established to prepare high-purity nervonic acid with the mixed fatty acids obtained after saponification and acidification of Acer truncatum Bunge oil as raw materials.First,according to the difference in the mean free path of fatty acids,molecular distillation was used to separate and remove C16 saturated fatty acid of palmitic acid and four C18-C20 fatty acids of stearic,oleic,linoleic,and linolenic acids.The content of C16-C20 fatty acids decreased from 72.92% to 19.22% after two-stage molecular distillation processes,in which the contents of saturated fatty acid of palmitic acid decreased to about 0.5%.Then,according to the difference in carbon chain length and saturation of fatty acid,the contents of C22-C24 saturated fatty acids of tetracosanoic and docosanoic acids decreased to 0.21% and 0.07% by urea inclusion with urea/free fatty acid preparation by saponification(SPOMFs)ratio as 0.6.In addition,all saturated fatty acids were basically separated.Finally,according to the difference in the solubility of fatty acids in solvents,the C18-C20 unsaturated fatty acids of oleic,linoleic,and linolenic acids and C22 unsaturated fatty acid of erucic acid were removed by solvent crystallization.The content of C18-C20 unsaturated fatty acids decreased to less than 5% with pentanol as the solvent after the first stage solvent crystallization.The content of erucic acid decreased to 3.47% with anhydrous ethanol as the solvent after the second to fifth stage solvent crystallization.The combined process of molecular distillation,urea inclusion and low temperature crystallization innovatively adopted an efficient,simple and easy-toindustrial solvent crystallization method to separate erucic and nervonic acids,obtaining nervonic acid with purity of 96.53% and final yield of 47.99%.展开更多
Two new species of Eurytoma,E.truncatumi Yang,Liu&Cao,sp.nov.and E.acutibialis Yang,Liu&Cao,sp.nov.,are described and illustrated,as the solitary larval and pupal ectoparasitoid of Bradybatus sp.(Coleoptera:Cu...Two new species of Eurytoma,E.truncatumi Yang,Liu&Cao,sp.nov.and E.acutibialis Yang,Liu&Cao,sp.nov.,are described and illustrated,as the solitary larval and pupal ectoparasitoid of Bradybatus sp.(Coleoptera:Curculionidae),a serious pest of purpleblow maple,Acer truncatum Bunge(Aceraceae).Photographs of the characters of the new species were provided.Typical materials were deposited in Insect Museum,Chinese Academy of Forestry,Beijing.The biological characters observed and their biocontrol potential were also discussed.展开更多
[ Objective] This study aimed to establish DNA fingerprints of 23 Acer truncatum clones, thus providing the theoretical basis for selection, classification and identification of A. truncatum varieties. [Metho...[ Objective] This study aimed to establish DNA fingerprints of 23 Acer truncatum clones, thus providing the theoretical basis for selection, classification and identification of A. truncatum varieties. [Method] Sixteen pairs of SRAP primers with rich polymorphism and high specificity were used to establish DNA fin-gerprints. [Result] A total of 223 bands were amplified with 16 primer pairs, including 197 polymorphic bands. Averagely 13.9 loci and 12.3 polymorphic loci were amplified with each primer pair. The average percentage of polymorphic loci reached 88. 34% . [ Conclusion] The classification result drawn by cluster analy-sis is consistent with that obtained based on main characteristics and genetic relationships of A. truncatum, clones. By using DNA fingerprints established with prim-er pairs ME1-EM4 and ME2-EM1, 23 A. truncatum clones can be effectively distinguished, and the confidence probability is greater than 99.99%.展开更多
Leaves of Acer truncatum ＇ Luhong No. 1 ＇ contain large amounts of polysaccharides and polyphenols, which seriously affect extraction yield and quality of total RNA. In order to explore the appropriate total RNA ext...Leaves of Acer truncatum ＇ Luhong No. 1 ＇ contain large amounts of polysaccharides and polyphenols, which seriously affect extraction yield and quality of total RNA. In order to explore the appropriate total RNA extraction method, total RNA was extracted from leaves of A. truncatum ＇ Luhong No. 1 ＇ with three methods, including kit method, Trizol method and modified CTAB method. The results showed that ODE6o/OD2so and OD^o/OD2ao ratios of total RNA extracted from leaves of A. truncatum ＇ Luhong No. 1 ＇ with kit method were higher than 1.8, with a general yield and certain level of DNA contamination ; OD^o/OD~ and OD^o/OD^o ratios of total RNA extracted with Trizol method were about 1.5, with the lowest yield; OD260/OD280 and OD260/OD230 ratios of total RNA extracted with modified CTAB method were about 2.0, with the highest yield and distinct eleetrophoresis patterns. The results demonstrated that total RNA extracted with modified CTAB method exhibited high yield and purity, which could meet the demands of subsequent molecular biology research. Thus, modified CTAB method is the appro- oriate method for extracting total RNA from leaves of A. truncatum ＇ Luhong No. 1 ＇展开更多
Plant volatile organic compounds (Biogenic Volatile Organic compounds, referred BVOCs) have a significant impact on the atmospheric environment, air quality and human health. This experiment takes Acer truncatum flowe...Plant volatile organic compounds (Biogenic Volatile Organic compounds, referred BVOCs) have a significant impact on the atmospheric environment, air quality and human health. This experiment takes Acer truncatum flowers as the research object, uses solid-phase micro-extraction combine GC-MS (SPME-GC-MS) to detect the main component of volatiles released by the flowers from 10 individual trees of Acer truncatum (Acer truncatum Bunge). The results showed that 37 kinds of volatiles were detected and they are belonged to four types organic compouds, such as terpenoids, alcohols, ketones, esters. According to the analysis of the main components of Acer truncatum flower volatiles includes Fluorene, 4,8 -Dimethyl-1,3 (E), 7-Nonene, (cis, trans)-2,6-Dimethyl-2,4,6-triene-Partenkirchen, Myrcene, Basil hexene, 3-Carene, (E)-Basil, Camphene, Caryophyllene, Linalool, α-Terpinolene, O-cymene, 3-Vinyl-1,2-dimethyl-1, Eucalyptus alcohols and Alcohol vinegar-12. However, there were no significant differences between individual trees in terms of obscure material O-cymene, Eucalyptus alcohols, Alcohol vinegar-12, as well as the significant differences in terms of remaining volatiles.展开更多
Acer truncatum is considered a promising species as a raw material to produce nervonic acid(NA). The goal of this study was to explore the variation of oil content and nervonic acid content of 138 accessions native to...Acer truncatum is considered a promising species as a raw material to produce nervonic acid(NA). The goal of this study was to explore the variation of oil content and nervonic acid content of 138 accessions native to 14 regions in China as well as provide guidance for establishing plantations and improving the production of nervonic acid. A large range of variation was found in oil content, nervonic acid per gram of oil, and nervonic acid per gram of seed(17.81%–36.56%, 3.90%–7.85%, and 0.84%–2.31%, respectively). Accessions YS-6, ABZ-6 and DQTL-8 were found as the most promising potential oil sources; PQ-2 was considered the optimal germplasm, and JY-6, NJ-8, LF-6, and CC-8 were found as promising potential sources for producing NA. A very significant difference(P < 0.01) among 14 regions was observed. DQTL and YS regions are considered the optimal farming regions for crops with high oil content, while the CC and PQ regions are regarded as the most suitable regions for nervonic acid production. A geographical trend from South to North was observed in which amount of seed oil increased but no such trend in nervonic acid content was observed. The data collected in the study on oil content and nervonic acid in accessions of A. truncatum in various regions can be utilized for establishing plantations of promising genotypes through clonal means.展开更多
基金Supported by Agricultural Elite Cultivar Project of Shandong Province(lkz2014[96])~~
文摘[Objective] Cloning of the AtrMYB transcription factor gene from Acer truncatum was conducted to further explore the red leaf development mechanism and breed cultivars of colored-leaf maple. [Method] The Acer truncatum ‘Luhong No.1＇ cultivar was used as the material for cloning the MYB gene by mean of RTPCR and RACE-PCR. [Results] Sequence analysis showed that the fragment contained a full coding region of 831 bp encoding 276 amino acid residues with a molecular weight of 32.17 kD and a molecular formula C_（1430）H_（14052）N_（2247）O_（406）S_（14）. The gene was named as AtrMYB with a Gen Bank accession number of 1825712. This coded protein had apI of 9.44. The results showed that the AtrMYB exhibited typical features of the R2R3-MYB domain. The AtrMYB was highly homologous with the MYB of other species at nucleotide and amino acid levels. The AtrMYB had no signal peptide, but a nuclear localization signal. The phylogenetic tree showed that the AtrMYB was at the same clade as the MYB from Citrus sinensis. [Conclusion] The AtrMYB was cloned from Acer truncatum ‘Luhong No.1＇ cultivar. These results have provided a foundation for further purification and identification of target protein and function study of the AtrMYB.
基金Supported by Agricultural Improved Variety Project of Shandong Province(LKZ[2014]No.96)
文摘[Objective] This study aimed to clone and analyze the sequence of CHS gene from Acer truncatum leaves. [Method] Using A. truncatum cultivars No.1-6 as experimental materials, total RNA was extracted from A. truncatum leaves with the modified CTAB method. CHS gene sequences were downloaded from the NCBI and aligned by BLAST. Degenerate primers were designed by DNAMAN and Primer- premier5 to amplify the target band. CHS gene fragment was amplified by RT-PCR and ligated to pMD18-T vector. The identified positive colonies were sequenced. [Result] A 1 365 bp fragment was amplified. Sequence analysis suggested that the obtained fragment encoded 365 amino acids and shared above 90% homology to nucleotide sequence of CHS gene from A. palmatum and A. [Conclusion] In this study, CHS gene was successfully cloned from A. truncatum for the first time, which laid the foundation for efficient utilization of CHS gene.
基金supported by the National Natural Science Foundation of China(22125802 and 22078010)Beijing Natural Science Foundation(2222017)Big Science Project from BUCT(XK180301).
文摘Nervonic acid is the world’s first and only potent substance that can repair damaged nerve fibers and promote nerve cell regeneration with high nutritional value.The wide variety of fatty acids in plant oils and fats with similar structures makes the large-scale separation and purification of high-purity nervonic acid very difficult.A new combined process of molecular distillation,urea inclusion and solvent crystallization was established to prepare high-purity nervonic acid with the mixed fatty acids obtained after saponification and acidification of Acer truncatum Bunge oil as raw materials.First,according to the difference in the mean free path of fatty acids,molecular distillation was used to separate and remove C16 saturated fatty acid of palmitic acid and four C18-C20 fatty acids of stearic,oleic,linoleic,and linolenic acids.The content of C16-C20 fatty acids decreased from 72.92% to 19.22% after two-stage molecular distillation processes,in which the contents of saturated fatty acid of palmitic acid decreased to about 0.5%.Then,according to the difference in carbon chain length and saturation of fatty acid,the contents of C22-C24 saturated fatty acids of tetracosanoic and docosanoic acids decreased to 0.21% and 0.07% by urea inclusion with urea/free fatty acid preparation by saponification(SPOMFs)ratio as 0.6.In addition,all saturated fatty acids were basically separated.Finally,according to the difference in the solubility of fatty acids in solvents,the C18-C20 unsaturated fatty acids of oleic,linoleic,and linolenic acids and C22 unsaturated fatty acid of erucic acid were removed by solvent crystallization.The content of C18-C20 unsaturated fatty acids decreased to less than 5% with pentanol as the solvent after the first stage solvent crystallization.The content of erucic acid decreased to 3.47% with anhydrous ethanol as the solvent after the second to fifth stage solvent crystallization.The combined process of molecular distillation,urea inclusion and low temperature crystallization innovatively adopted an efficient,simple and easy-toindustrial solvent crystallization method to separate erucic and nervonic acids,obtaining nervonic acid with purity of 96.53% and final yield of 47.99%.
基金financially supported by the Key Project of the Fundamental Fund of the Chinese Academy of Forestry(CAFYBB2020ZB005)。
文摘Two new species of Eurytoma,E.truncatumi Yang,Liu&Cao,sp.nov.and E.acutibialis Yang,Liu&Cao,sp.nov.,are described and illustrated,as the solitary larval and pupal ectoparasitoid of Bradybatus sp.(Coleoptera:Curculionidae),a serious pest of purpleblow maple,Acer truncatum Bunge(Aceraceae).Photographs of the characters of the new species were provided.Typical materials were deposited in Insect Museum,Chinese Academy of Forestry,Beijing.The biological characters observed and their biocontrol potential were also discussed.
基金Supported by Agricultural Improved Variety Project of Shandong Province(LKZ[2012]No.213,LKZ[2014]No.96)
文摘[ Objective] This study aimed to establish DNA fingerprints of 23 Acer truncatum clones, thus providing the theoretical basis for selection, classification and identification of A. truncatum varieties. [Method] Sixteen pairs of SRAP primers with rich polymorphism and high specificity were used to establish DNA fin-gerprints. [Result] A total of 223 bands were amplified with 16 primer pairs, including 197 polymorphic bands. Averagely 13.9 loci and 12.3 polymorphic loci were amplified with each primer pair. The average percentage of polymorphic loci reached 88. 34% . [ Conclusion] The classification result drawn by cluster analy-sis is consistent with that obtained based on main characteristics and genetic relationships of A. truncatum, clones. By using DNA fingerprints established with prim-er pairs ME1-EM4 and ME2-EM1, 23 A. truncatum clones can be effectively distinguished, and the confidence probability is greater than 99.99%.
基金Supported by Project of Agricultural Fine Varieties of Shandong Province[LKNZ(2012)No.213]
文摘Leaves of Acer truncatum ＇ Luhong No. 1 ＇ contain large amounts of polysaccharides and polyphenols, which seriously affect extraction yield and quality of total RNA. In order to explore the appropriate total RNA extraction method, total RNA was extracted from leaves of A. truncatum ＇ Luhong No. 1 ＇ with three methods, including kit method, Trizol method and modified CTAB method. The results showed that ODE6o/OD2so and OD^o/OD2ao ratios of total RNA extracted from leaves of A. truncatum ＇ Luhong No. 1 ＇ with kit method were higher than 1.8, with a general yield and certain level of DNA contamination ; OD^o/OD~ and OD^o/OD^o ratios of total RNA extracted with Trizol method were about 1.5, with the lowest yield; OD260/OD280 and OD260/OD230 ratios of total RNA extracted with modified CTAB method were about 2.0, with the highest yield and distinct eleetrophoresis patterns. The results demonstrated that total RNA extracted with modified CTAB method exhibited high yield and purity, which could meet the demands of subsequent molecular biology research. Thus, modified CTAB method is the appro- oriate method for extracting total RNA from leaves of A. truncatum ＇ Luhong No. 1 ＇
文摘Plant volatile organic compounds (Biogenic Volatile Organic compounds, referred BVOCs) have a significant impact on the atmospheric environment, air quality and human health. This experiment takes Acer truncatum flowers as the research object, uses solid-phase micro-extraction combine GC-MS (SPME-GC-MS) to detect the main component of volatiles released by the flowers from 10 individual trees of Acer truncatum (Acer truncatum Bunge). The results showed that 37 kinds of volatiles were detected and they are belonged to four types organic compouds, such as terpenoids, alcohols, ketones, esters. According to the analysis of the main components of Acer truncatum flower volatiles includes Fluorene, 4,8 -Dimethyl-1,3 (E), 7-Nonene, (cis, trans)-2,6-Dimethyl-2,4,6-triene-Partenkirchen, Myrcene, Basil hexene, 3-Carene, (E)-Basil, Camphene, Caryophyllene, Linalool, α-Terpinolene, O-cymene, 3-Vinyl-1,2-dimethyl-1, Eucalyptus alcohols and Alcohol vinegar-12. However, there were no significant differences between individual trees in terms of obscure material O-cymene, Eucalyptus alcohols, Alcohol vinegar-12, as well as the significant differences in terms of remaining volatiles.
基金supported by Central Fiscal Forestry Science and Technology Extension Demonstration Fund Project(No.2016LZGC014)a Major Agricultural Project of Shandong Province(No.[2012] 213 and [2014] 96)Shandong "Double Tops" Program
文摘Acer truncatum is considered a promising species as a raw material to produce nervonic acid(NA). The goal of this study was to explore the variation of oil content and nervonic acid content of 138 accessions native to 14 regions in China as well as provide guidance for establishing plantations and improving the production of nervonic acid. A large range of variation was found in oil content, nervonic acid per gram of oil, and nervonic acid per gram of seed(17.81%–36.56%, 3.90%–7.85%, and 0.84%–2.31%, respectively). Accessions YS-6, ABZ-6 and DQTL-8 were found as the most promising potential oil sources; PQ-2 was considered the optimal germplasm, and JY-6, NJ-8, LF-6, and CC-8 were found as promising potential sources for producing NA. A very significant difference(P < 0.01) among 14 regions was observed. DQTL and YS regions are considered the optimal farming regions for crops with high oil content, while the CC and PQ regions are regarded as the most suitable regions for nervonic acid production. A geographical trend from South to North was observed in which amount of seed oil increased but no such trend in nervonic acid content was observed. The data collected in the study on oil content and nervonic acid in accessions of A. truncatum in various regions can be utilized for establishing plantations of promising genotypes through clonal means.
