A series of new pyrazole oximes bearing substituted thiazole ring were designed and prepared. The structures of the title compounds were identified by spectral analyses, The results of primary bioassay indicated that ...A series of new pyrazole oximes bearing substituted thiazole ring were designed and prepared. The structures of the title compounds were identified by spectral analyses, The results of primary bioassay indicated that some targeted compounds exhibited promising insecticidal activity besides acaricidal activity, particularly; compounds 8c and 8d were more potent against Tetranychus cinnabarinus and Plutella xylostella than other analogues.展开更多
Objective:To examine the acaricidal effects of the essential oil of Cymbopogon citratits leaf extract(lemongrass)and ethanolic Azadirachta indica leaf extract(neem)against house dust mites Dermatophagoides farinae(D.f...Objective:To examine the acaricidal effects of the essential oil of Cymbopogon citratits leaf extract(lemongrass)and ethanolic Azadirachta indica leaf extract(neem)against house dust mites Dermatophagoides farinae(D.farinae)and Dermalophagoides pteronyssinus(D.pteronyssinus).Methods:Twenty-five adults mites were placed onto treated filter paper that is soaked with plant extract and been tested at different concentrations(50.00%,25.00%,12.50%,6.25%and 3.13%)and exposure times(24hrs,48hrs,72hrs and 96 hrs).All treatments were replicated 7 times,and the experiment repeated once.The topical and contact activities of the two herbs were investigated.Results:Mortalities from lemongrass extract were higher than neem for both topical and contact activities.At 50%concentration,both 24 hrs topical and contact exposures to lemongrass resulted in more than 91%mortalities for both species of inites.At the same concentration and exposure time,neem resulted in topical mortalities of 40.3%and 15.7%against D.pteronyssinus and D.farinae respectively;contact mortalities were 8.0%and 8.9%against the 2 mites,respectively.There was no difference in topical mortalities of D.pteronyssinus from exposure to concentrations oflemongrass and neem up to 12.50%;lemongrass was more effective than neem at the higher concentrations.Conclusions:Generally,topical mortalities of D.farinae due to lemongrass are higher than that due to neem.Contact mortalities of lemongrass are always higher that neem against both species of mites.展开更多
Objective: To evaluate the acaricidal activity of the essential oil obtained from roots of Derris floribunda(D. floribunda)(Miq.) Benth, and its main constituent nerolidol against the Mexican mite Tetranychus mexicanu...Objective: To evaluate the acaricidal activity of the essential oil obtained from roots of Derris floribunda(D. floribunda)(Miq.) Benth, and its main constituent nerolidol against the Mexican mite Tetranychus mexicanus(T. mexicanus)(Mc Gregor).Methods: The essential oil from the roots of D. floribunda collected in the Amazon region(Brazil) was obtained by hydrodistillation. Its chemical composition was determined by GC–MS analysis. The acaricidal activities of this essential oil and nerolidol,were evaluated by recording the number of dead females(mortality) and eggs(fertility).Results: The essential oil showed sesquiterpenes as major volatile components. Nerolidol, the main component, represented 68.5% of the total composition of the essential oil. D. floribunda essential oil and nerolidol showed acaricidal activity, with LC_(50) of9.61 mg/m L air and 9.2 mg/m L air, respectively, over a 72 h period. In addition, both the essential oil and nerolidol significantly reduced the fecundity of T. mexicanus.Conclusions: Due to the economic importance of T. mexicanus and the lack of new pesticides, our data are very promising in the search for efficient and safer acaricidal products. Furthermore, this is the first report about the chemical composition and bioactivity of the essential oil of the Amazon plant species D. floribunda.展开更多
Acaricidal activity of Boenninghausenia sessilicarpa against Panonychus cirri was tested in the laboratory. Four solvents were used to prepare crude extracts, petroleum ether, chloroform, ethyl acetate, and ethanol, a...Acaricidal activity of Boenninghausenia sessilicarpa against Panonychus cirri was tested in the laboratory. Four solvents were used to prepare crude extracts, petroleum ether, chloroform, ethyl acetate, and ethanol, among which ethanol was the most effective one. The results suggested that ethanol extracts of B. sessilicarpa had eminent acaricidal and ovicidal activities. Concentrated extracts were prepared using petroleum ether, chloroform, ethyl acetate, or distilled water as solvent. Mite mortality rates in the concentrated extracts by petroleum ether, chloroform, or distilled water were significantly lower than those by ethyl acetate. The LC50 values of eggs and female mites were 0.7639 and 1.1033 mg mL^-1, respectively. After liquid chromatography and thin layer chromatography, the concentrated extracts were separated into 14 groups of fractions and further tests for their acaricidal and ovicidal activities were conducted. Fraction 2 was found to possess higher acaricidal and ovicidal activities. The mortality of eggs and adult mites were 85.83 and 63.07%, respectively. Moreover, fraction 2 showed moderate oviposition inhibition effect （0.8795） against P. citri when the used dose was higher than 2.5 mg mL^-1展开更多
Pyriminostrobin, a new acaricide, was discovered in our previous studies. Because introducing fluorine into organic compounds can increase bioactivity, pyriminostrobin was modified as a series of strobilurin-pyrimidin...Pyriminostrobin, a new acaricide, was discovered in our previous studies. Because introducing fluorine into organic compounds can increase bioactivity, pyriminostrobin was modified as a series of strobilurin-pyrimidine derivatives for biological screening. The compounds were characterized by 1H NMR, MS and elemental analysis. Preliminary bioassays demonstrated that compounds 7e and 7i exhibited significant control against Tetranychus cinnabarinus （Boisd.） at 0.625 mg L^-1, and their acaricidal potencies were higher than pyriminostrobin in a greenhouse. The relationship between structure and acaricidal activity was also studied.展开更多
Objective:To green synthesize and characterize copper nanoparticles(Cu NPs)using Astragalus sinicus,as well as evaluate the acaricidal,larvacidal,and repellent activities of Cu NPs against Hyalomma anatolicum(H.anatol...Objective:To green synthesize and characterize copper nanoparticles(Cu NPs)using Astragalus sinicus,as well as evaluate the acaricidal,larvacidal,and repellent activities of Cu NPs against Hyalomma anatolicum(H.anatolicum),one of the most prevalent ticks infesting cattle in Saudi Arabia.Methods:Cu NPs were green synthesized by adding the Astragalus sinicus extract to a copper sulfate solution.The acaricidal,larvicidal,and repellent activities of Cu NPs against H.anatolicum were assessed via the adult immersion test,the larval packet test,and the vertical movement behavior of tick larvae,respectively.The effects of Cu NPs on acetylcholinesterase as well as oxidative enzyme activities were examined.Results:The green synthesized Cu NPs displayed a spherical form with a size range of 15-75 nm.After exposure of adult H.anatolicum to different concentrations of Cu NPs,the viability rate of adult H.anatolicum and the mean number,weight,and hatchability of eggs were noticeably reduced,in comparison to the control group(P<0.001).In addition,the viability rate of larvae considerably declined(P<0.001)with the LC_(50)and LC_(90)values of 11.30 and 20.34μg/m L,respectively.The maximum repellent activity of Cu NPs was observed at 50,100,and 200μg/m L with complete repellent activity after 60,120,and 180 min of exposure,respectively.Cu NPs,mainly at 1/2 LC_(50)and LC_(50)concentrations,markedly suppressed the acetylcholinesterase activity of the larval stage of H.anatolicum(P<0.001).Moreover,Cu NPs,mainly at LC_(50)dose,significantly elevated malondialdehyde level while declining glutathione-S-transferase level in H.anatolicum larvae(P<0.001).Conclusions:Cu NPs show potent acaricidal,larvicidal,and repellent activities against adults and larvae of H.anatolicum.However,further studies must be performed to clarify the precise mechanisms and the efficacy of Cu NPs in practical use.展开更多
Tricyclohexyltin 4-amino-3-methyl-benzoate （1） and tricyclohexyltin 3,4-dime- thoxybenzoate （2） were synthesized and their crystal structures were determined by X-ray diffraction. Compound 1 belongs to the monocli...Tricyclohexyltin 4-amino-3-methyl-benzoate （1） and tricyclohexyltin 3,4-dime- thoxybenzoate （2） were synthesized and their crystal structures were determined by X-ray diffraction. Compound 1 belongs to the monoclinic system, space group P21/c with a = 14.0554（15）, b = 11.6947（13）, c = 16.2720（17）A, Z = 4, V= 2565.7（5） A3, Dc = 1.341 g.cm-3,μ（MoKa） = 1.016 mm-1, F（000） = 1080, R = 0.0833 and wR = 0.2964. Compound 2 is of monoclinic system, space group C2/c with a = 17.8712（6）, b = 8.4433（3）, c = 35.5783（13） A, Z = 8, V= 5366.8（3） A3, Dc = 1.360 g.cm-3,/1（MoKa） = 0.980 mm-1, F（000） = 2288, R = 0.0549, wR = 0.1279. In compounds 1 and 2, the central Sn atom is coordinated in a tetradentate manner to assume a distorted tetrahedral configuration. Preliminary biological tests showed that these two compounds have strong acaricidal activity.展开更多
Hyoscyamus niger L. （Solanaceae） and Hypericum calycinum L. （Hypericaceae） were collected from two provinces Bursa and Ankara in Turkey during flowering stages. Extracts obtained from H. niger and H. calycinum wer...Hyoscyamus niger L. （Solanaceae） and Hypericum calycinum L. （Hypericaceae） were collected from two provinces Bursa and Ankara in Turkey during flowering stages. Extracts obtained from H. niger and H. calycinum were tested against two-spotted mite as an acaricidal pesticide. Bioassay experiments were carried out under laboratory conditions using two different methods namely, leaf disc dipping and direct leaf spraying. Two-spotted mite was reared in insectary using Phaseolus vulgaris L. （Fabaceae）. In the leaf dipping method, H. niger extract resulted in an effective adjusted mortality for larvae of 87.13% and for adults of 81.66%. For H. calycinum the adjusted mortality for larvae and adults was 77.77% and 85.97% respectively at the highest concentration. In larval stage, LCs0 values were 5.47 w/v and 3.32 w/v dipping and spraying method respectively. In addition, adult stage, it was determined that LCs0 values were 1.17 w/v and 3.56 w/v in both methods. For leaf spraying method. H. niger resulted in an adjusted showed an effect against larvae and adults mortality rate was 95% and 91% respectively. However, H. calycinum mortality percentage was 80% and 87% at 12% concentration, with LC50 values of 9.62 w/v and 2.13 w/v in dipping method. In this research apart from mortality effects on egg, reproduction was also investigated. Each concentration showed that treated females laid less eggs compared to the untreated females.展开更多
Two-spotted spider mite(Tetranychus urticae Koch)(Acari:Tetranychidae)is a very serious pest worldwide,causing considerable damage to vegetables,flowers and fruit crops.The application of plant extracts to control ins...Two-spotted spider mite(Tetranychus urticae Koch)(Acari:Tetranychidae)is a very serious pest worldwide,causing considerable damage to vegetables,flowers and fruit crops.The application of plant extracts to control insects can be an effective alternative.In this study,the efficacy of an extract from Juglans regia L.(Juglandecaea)was tested as an alternative to conventional acaricides.Using two different methods,bioassays were used to determine the effects of varying concentrations J.regia(1%,3%,6%,12%)of the extracts.Experiments were carried out using 3 cm diameter leaf disks of Phaseolus vulgaris L.In addition,the effects of the extract on mite reproduction and oviposition were investigated.As a result,in the leaf dipping method,the 12%concentration of the extract caused the highest mortality of nymph(90%)and adult(83.00%)stages.For the spraying method,the mortality of adults at the same concentration was 100%.In bioassays at lower concentrations,the adult mites laid lower numbers of eggs in comparison to the untreated mites.The highest effect occurred at concentration of 12%and the lowest effect was at 1%.The extract was no observed ovicidal effect.展开更多
The acaricidal activities of fourteen essential oils and fourteen of their major monoterpenoids were tested against house dust mites Dermatophagoides pteronyssinus. Five concentrations were used over two different tim...The acaricidal activities of fourteen essential oils and fourteen of their major monoterpenoids were tested against house dust mites Dermatophagoides pteronyssinus. Five concentrations were used over two different time intervals 24 and 48 h under laboratory conditions. In general, it was noticed that the acaricidal effect based on LC50 of either essential oils or monoterpenoids against the mite was time dependant. The LCso values were decreased by increasing of exposure time. Clove, matrecary, chenopodium, rosemary, eucalyptus and caraway oils were shown to have high activity. As for the monoterpenoids, cinnamaldehyde and chlorothymol were found to be the most effective followed by citronellol. This study suggests the use of the essential oils and their major constituents as ecofriendly biodegradable agents for the control of house dust mite, D. pteronyssinus.展开更多
To search for novel 2-arylpyrroles with unique biological activities,a series of novel 2-arylpyrrole derivatives were designed and synthesized,and their structures were characterized by 1 H and 13 C NMR spectroscopy,M...To search for novel 2-arylpyrroles with unique biological activities,a series of novel 2-arylpyrrole derivatives were designed and synthesized,and their structures were characterized by 1 H and 13 C NMR spectroscopy,MS spectrometry,and elemental analysis.Their insecticidal activities against Lepidopteran pests (e.g.Mythimna separata) and acaricidal activities against mites (e.g.Tetranychus urticae) were evaluated.The results of bioassays indicate that some of these title compounds exhibited excellent insecticidal and acaricidal activities.For example,4-bromo-1-((chloromethoxy)methyl)-2-(4-chloro phenyl)-5-(trifluoromethyl)pyrrole-3-carbonitrile (6a),4-bromo-2-(4-chlorophenyl)-1-((2-fluoroethoxy)-methyl)-5-(trifluoromethyl)pyrrole-3-carbonitrile (6d) showed insecticidal activity against Mythimna separata and 4-bromo-2-(4-chlorophenyl)1-((isopropoxymethoxy)methyl)-5-(trifluoro methyl)pyrrole-3-carbonitrile (7d) showed acaricidal activity against Tetranychus urticae.They were more effective than Chlorfenapyr,which has been the only commercialized member of a new class of chemicals of 2-arylpyrroles.展开更多
This study compared the control effect of 110 g/L etoxazole SC,15%abamectin·etoxazole SC,30%cyetpyrafen SC,43%bifenazate SC and 1.8%abamectin EC five acaricides sprayed by unmanned aerial vehicle(UAV)on Panonychu...This study compared the control effect of 110 g/L etoxazole SC,15%abamectin·etoxazole SC,30%cyetpyrafen SC,43%bifenazate SC and 1.8%abamectin EC five acaricides sprayed by unmanned aerial vehicle(UAV)on Panonychus citri,aiming to screen out the appropriate acaricide for the control of this pest by UAV spraying.The results showed that 15%abamectin·etoxazole SC and 30%cyetpyrafen SC had the highest control efficacy,which remained above 90%14 d after application.Secondary performance was observed in 43%bifenazate SC and 110 g/L etoxazole SC,which demonstrated enhancing control effect.However,1.8%abamectin EC showed slower effect.Considering the control effect and population reduction rate of P.citri,15%abamectin·etoxazole SC and 30%cyetpyrafen SC were suggested as the effective acaricides for the control of this pest.展开更多
[Objective] This study aimed to compare the effects of sublimed sulfur and acaricide on controlling bee mites.[Method] Three independent experiments were carried out in the same apiary during 2007-2009.[Results] The r...[Objective] This study aimed to compare the effects of sublimed sulfur and acaricide on controlling bee mites.[Method] Three independent experiments were carried out in the same apiary during 2007-2009.[Results] The results showed that this method is effective in eliminating mites settled on combs or hidden during pupae phase,but not in controlling the mites parasitized on adult bees.The control effect on killing Varroa destructor after treated appeared optimal in the first day and then decreased,and that on killing Tvopilaelaps clareae happened in the second day.The results indicated that the effect would be much better if the bees can be treated 1 time to 2 times again in the following 2-5 days after the first treatment.Back to data pool,we found that the treatment of sublimed sulfur paired with acaricide is effective in controlling V.destructor and T.clareae.[Conclusion] This technique could facilitate the apicultural production in the regions suffered from V.destructor and T.clareae.展开更多
A series of novel pyrazole oxime derivatives containing oxazole ring were designed and synthesized. The title compounds were structurally confirmed by 1H NMR, 13C NMR spectra and elemental analyses. Preliminary bioass...A series of novel pyrazole oxime derivatives containing oxazole ring were designed and synthesized. The title compounds were structurally confirmed by 1H NMR, 13C NMR spectra and elemental analyses. Preliminary bioassay results showed that some of the title compounds displayed promising fungicidal activity besides insecticidal and acaricidal activity. Particularly, compound 8c exhibited potent fungicidal activity against cucumber Pseudoperonospora cubensis beyond good insecticidal activity against Aphis craccivora and Nilaparvata lugens.展开更多
A novel synthesis of 2,3-dihydroimidazo[1,2-a]pyridin-5 (1H)-one 4 and its derivatives were described.Preliminary bioassays showed that some of the target compounds exhibited excellent insecticidal activities against ...A novel synthesis of 2,3-dihydroimidazo[1,2-a]pyridin-5 (1H)-one 4 and its derivatives were described.Preliminary bioassays showed that some of the target compounds exhibited excellent insecticidal activities against brown planthopper (Nilaparvata lugens), cowpea aphids (Aphis craccivora) (4,5 a, 5 c, 5 g,5 h, 5 j, 5 r, 6 b, 6 e) and carmine spider mite (Tetranychus cinnabarinus (5 f, 5 s, 6 a) at 500 mg/L. Among them,compound 4 was still active against brown planthopper and cowpea aphids at 4 mg/L. The insecticidal activities were influenced by the types and position of the substituents, which provided guidance for the structure modifications.展开更多
Sixteen new derivatives of spiromesifen were synthesized from 3-（2,4,6-trimethylphenyl）-4-hydroxy-△^3-dihydrofuran-2-one. Their bioactivities against diamondbackmoth （Plutella xylostella） and spider mites （Tetra...Sixteen new derivatives of spiromesifen were synthesized from 3-（2,4,6-trimethylphenyl）-4-hydroxy-△^3-dihydrofuran-2-one. Their bioactivities against diamondbackmoth （Plutella xylostella） and spider mites （Tetranychus cinnabarinus） were also evaluated. The structures of these derivatives were confirmed by ^1H NMR, MS.展开更多
Six new 13-O-acylavermectin Bl aglycones(3-8) were synthesized from avermectin B1 aglycone and their bioactivities were evaluated against Spodoptera exigua, Spodoptera eridania, Tetranychus urticae and Aphis fabae.
Groundwater in Egypt plays an important role in the country＇s water budget. South Western desert represent an arid of desert biome within world＇s net-work of the biosphere reserves. In this study, the plant wild veg...Groundwater in Egypt plays an important role in the country＇s water budget. South Western desert represent an arid of desert biome within world＇s net-work of the biosphere reserves. In this study, the plant wild vegetation were surveyed in Abu Tartur mining area located in the Southern part of Western depending essentially on the seepage from water line tubes which supplies water demands at Abu Tartur. The vegetation-environment relationships in Abu Tartur are described. Data sets （42 species in 38 plots） beside the pip-line enriched from 13 wells were analyzed, using multivariate procedures, i.e., two-way indicator species analysis （TW1NSPAN）, detrended correspondence analysis （DCA） and detrended canonical correspondence analysis （CCA）, to produce a classification of plant communities in the studied areas and to examine the relationships of that plant communities to certain edaphic factors. Five plant communities were identified. Astragalus vogelii, Chenopodium murale, Citrullus colocynthis, Fagonia arabica, Farsetia aegyptia, Hyoscyamus muticus, Morettia philaeana, Cynodon dactylon, Trichodesma africana, Tarnarix nilotica, Senna italica, Schouwia purpurea, Salsola volkensii and Phragmites australis were common in the study area. Phytochemical survey for nitroprpionic compounds in some taxa belonging to Fabaceae family showed the absence of these toxic compounds by using GC/MS analysis. Also some phytochemical components ofAstragalus vogalii were extracted and identified by GC/MS spectra. A biological activity, in this regard was the screening of methanol extracts for some wild taxa of Abu Tartur against natural Tetranychus urticae as acricidal activity and the methanol extracts of some taxa give high mortality results, like Farsetia aegyptia （86.6%） and Fagonia arabica Burm. f. （70.0%）.展开更多
This study reports the extraction of <i>Jatropha curcas</i> leaves using supercritical CO<sub>2</sub>. Experiments were performed varying the pressure (13 and 20 MPa) and the temperature (50...This study reports the extraction of <i>Jatropha curcas</i> leaves using supercritical CO<sub>2</sub>. Experiments were performed varying the pressure (13 and 20 MPa) and the temperature (50°C and 60°C). The model of Sovová for supercritical fluid extraction was fitted to the experimental kinetic extraction curves. Two cell sizes were used and scale up equations compared. GC analysis showed phytol, carvacrol, and hexahydrofarnesyl acetone as major compounds in all the experiments. A maximum yield of 0.95% dry-weight basis was obtained. It was observed a maximum yield (0.95% dry-weight basis) extract obtained at 20 MPa and 50°C. The results indicated that the mass yield increased with the increase of pressure. The bioassays showed that the extract of <i>J. curcas</i> possessed toxicity against <i>Hyalomma lusitanicum</i>.展开更多
In the present work, the potential of acaricidal activity of chamomile flowers＇ extract was studied against engorged Rhipicephalus annulatus tick under laboratory condition. For this purpose, the engorged females ofR...In the present work, the potential of acaricidal activity of chamomile flowers＇ extract was studied against engorged Rhipicephalus annulatus tick under laboratory condition. For this purpose, the engorged females ofRhipicephalus annulatus were exposed to two-fold serial dilutions of chamomile flowers＇ extract （0.5%, 1.0%, 2.0%, 4.0% and 8.0%） using ＂dipping method＂ in vitro. The engorged ticks were immersed in different plant dilutions （five ticks for each dilution） for l rain and they were immediately incubated in separate Petri dishes for each replicate at 26℃ and 80% relative humidity. Mortality rate for each treatment was recorded 5 d after incubation. The mortality rate caused by different dilutions of chamomile flowers＇ extract ranged from 6.67% to 26.7%, whereas no mortality was recorded for non-treated control group. The mass of produced eggs varied from 0.23 g （in 8.0% solution） to 0.58 g （in control）, with no statistical differences between the treatments and control （/＇〉0.05）. Also the chamomile flowers＇ extract in highest concentration used （8.0%） caused 46.67% failure in egg laying in engorged females while no failure was observed for non-treated control group. Macroscopic observations indicated that in effective concentrations of plant （4.0% and 8.0%）, patchy hemorrhagic swelling appeared on the skin of treated ticks. The results presented for the first time in this study imply that chamomile may be considered as a promising plant for biocontrol of cattle fever tick disease in the field condition.展开更多
基金financial supported by the National Natural Science Foundation of China(No.21202089)China Postdoctoral Science Foundation(No.2013M531145)the Research Foundation of the Six People Peak of Jiangsu Province(No.2013-SWYY-013)
文摘A series of new pyrazole oximes bearing substituted thiazole ring were designed and prepared. The structures of the title compounds were identified by spectral analyses, The results of primary bioassay indicated that some targeted compounds exhibited promising insecticidal activity besides acaricidal activity, particularly; compounds 8c and 8d were more potent against Tetranychus cinnabarinus and Plutella xylostella than other analogues.
文摘Objective:To examine the acaricidal effects of the essential oil of Cymbopogon citratits leaf extract(lemongrass)and ethanolic Azadirachta indica leaf extract(neem)against house dust mites Dermatophagoides farinae(D.farinae)and Dermalophagoides pteronyssinus(D.pteronyssinus).Methods:Twenty-five adults mites were placed onto treated filter paper that is soaked with plant extract and been tested at different concentrations(50.00%,25.00%,12.50%,6.25%and 3.13%)and exposure times(24hrs,48hrs,72hrs and 96 hrs).All treatments were replicated 7 times,and the experiment repeated once.The topical and contact activities of the two herbs were investigated.Results:Mortalities from lemongrass extract were higher than neem for both topical and contact activities.At 50%concentration,both 24 hrs topical and contact exposures to lemongrass resulted in more than 91%mortalities for both species of inites.At the same concentration and exposure time,neem resulted in topical mortalities of 40.3%and 15.7%against D.pteronyssinus and D.farinae respectively;contact mortalities were 8.0%and 8.9%against the 2 mites,respectively.There was no difference in topical mortalities of D.pteronyssinus from exposure to concentrations oflemongrass and neem up to 12.50%;lemongrass was more effective than neem at the higher concentrations.Conclusions:Generally,topical mortalities of D.farinae due to lemongrass are higher than that due to neem.Contact mortalities of lemongrass are always higher that neem against both species of mites.
基金financially supported by Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM – PAPAC program EDITAL N. 015/2014)
文摘Objective: To evaluate the acaricidal activity of the essential oil obtained from roots of Derris floribunda(D. floribunda)(Miq.) Benth, and its main constituent nerolidol against the Mexican mite Tetranychus mexicanus(T. mexicanus)(Mc Gregor).Methods: The essential oil from the roots of D. floribunda collected in the Amazon region(Brazil) was obtained by hydrodistillation. Its chemical composition was determined by GC–MS analysis. The acaricidal activities of this essential oil and nerolidol,were evaluated by recording the number of dead females(mortality) and eggs(fertility).Results: The essential oil showed sesquiterpenes as major volatile components. Nerolidol, the main component, represented 68.5% of the total composition of the essential oil. D. floribunda essential oil and nerolidol showed acaricidal activity, with LC_(50) of9.61 mg/m L air and 9.2 mg/m L air, respectively, over a 72 h period. In addition, both the essential oil and nerolidol significantly reduced the fecundity of T. mexicanus.Conclusions: Due to the economic importance of T. mexicanus and the lack of new pesticides, our data are very promising in the search for efficient and safer acaricidal products. Furthermore, this is the first report about the chemical composition and bioactivity of the essential oil of the Amazon plant species D. floribunda.
基金supported by the Special Public Sector Research of China (nyhyzx07-057)the National Key Technology R&D Program of China(2007BAD47B04, 2008BAD92B08)
文摘Acaricidal activity of Boenninghausenia sessilicarpa against Panonychus cirri was tested in the laboratory. Four solvents were used to prepare crude extracts, petroleum ether, chloroform, ethyl acetate, and ethanol, among which ethanol was the most effective one. The results suggested that ethanol extracts of B. sessilicarpa had eminent acaricidal and ovicidal activities. Concentrated extracts were prepared using petroleum ether, chloroform, ethyl acetate, or distilled water as solvent. Mite mortality rates in the concentrated extracts by petroleum ether, chloroform, or distilled water were significantly lower than those by ethyl acetate. The LC50 values of eggs and female mites were 0.7639 and 1.1033 mg mL^-1, respectively. After liquid chromatography and thin layer chromatography, the concentrated extracts were separated into 14 groups of fractions and further tests for their acaricidal and ovicidal activities were conducted. Fraction 2 was found to possess higher acaricidal and ovicidal activities. The mortality of eggs and adult mites were 85.83 and 63.07%, respectively. Moreover, fraction 2 showed moderate oviposition inhibition effect （0.8795） against P. citri when the used dose was higher than 2.5 mg mL^-1
基金the Outstanding Young Scholarship from the National Natural Science Foundation of China(No.30825043)NSFC(No.21172202)+2 种基金the Outstanding Scholar Foundation of Henan Province(No.094100510019)for financial supportsupported by the National Key Basic Research Program of China(973 Program)(Nos.2010CB126105 and 2012CB724501)the National Key Technology Support Program during the 12th Five-Year Plan Period(Nos.2011BAE06B00,2011BAE06B01,2011BAE06B02,2011BAE06B03 and 2011BAE06B05)
文摘Pyriminostrobin, a new acaricide, was discovered in our previous studies. Because introducing fluorine into organic compounds can increase bioactivity, pyriminostrobin was modified as a series of strobilurin-pyrimidine derivatives for biological screening. The compounds were characterized by 1H NMR, MS and elemental analysis. Preliminary bioassays demonstrated that compounds 7e and 7i exhibited significant control against Tetranychus cinnabarinus （Boisd.） at 0.625 mg L^-1, and their acaricidal potencies were higher than pyriminostrobin in a greenhouse. The relationship between structure and acaricidal activity was also studied.
文摘Objective:To green synthesize and characterize copper nanoparticles(Cu NPs)using Astragalus sinicus,as well as evaluate the acaricidal,larvacidal,and repellent activities of Cu NPs against Hyalomma anatolicum(H.anatolicum),one of the most prevalent ticks infesting cattle in Saudi Arabia.Methods:Cu NPs were green synthesized by adding the Astragalus sinicus extract to a copper sulfate solution.The acaricidal,larvicidal,and repellent activities of Cu NPs against H.anatolicum were assessed via the adult immersion test,the larval packet test,and the vertical movement behavior of tick larvae,respectively.The effects of Cu NPs on acetylcholinesterase as well as oxidative enzyme activities were examined.Results:The green synthesized Cu NPs displayed a spherical form with a size range of 15-75 nm.After exposure of adult H.anatolicum to different concentrations of Cu NPs,the viability rate of adult H.anatolicum and the mean number,weight,and hatchability of eggs were noticeably reduced,in comparison to the control group(P<0.001).In addition,the viability rate of larvae considerably declined(P<0.001)with the LC_(50)and LC_(90)values of 11.30 and 20.34μg/m L,respectively.The maximum repellent activity of Cu NPs was observed at 50,100,and 200μg/m L with complete repellent activity after 60,120,and 180 min of exposure,respectively.Cu NPs,mainly at 1/2 LC_(50)and LC_(50)concentrations,markedly suppressed the acetylcholinesterase activity of the larval stage of H.anatolicum(P<0.001).Moreover,Cu NPs,mainly at LC_(50)dose,significantly elevated malondialdehyde level while declining glutathione-S-transferase level in H.anatolicum larvae(P<0.001).Conclusions:Cu NPs show potent acaricidal,larvicidal,and repellent activities against adults and larvae of H.anatolicum.However,further studies must be performed to clarify the precise mechanisms and the efficacy of Cu NPs in practical use.
基金supported by the Natural Science Foundation of Hunan Province(No.13JJ3112)Scientific & Technological Projects of Hunan Province(No.2014FJ3060)+4 种基金the Open Fund Project of Key Laboratory in Hunan Universities(No.13K105)Scientific Research Fund of Hunan Provincial Education Department of China(No.12C0537)the Construct Program of the Key Discipline in Hunan Province,Scientific & Technological Projects of Hengyang city(2011KG56,2012KJ30)Science Foundation of Hengyang Normal University(No.12C45)the Youth Backbone Teacher Training Program of Hengyang Normal University(2012)
文摘Tricyclohexyltin 4-amino-3-methyl-benzoate （1） and tricyclohexyltin 3,4-dime- thoxybenzoate （2） were synthesized and their crystal structures were determined by X-ray diffraction. Compound 1 belongs to the monoclinic system, space group P21/c with a = 14.0554（15）, b = 11.6947（13）, c = 16.2720（17）A, Z = 4, V= 2565.7（5） A3, Dc = 1.341 g.cm-3,μ（MoKa） = 1.016 mm-1, F（000） = 1080, R = 0.0833 and wR = 0.2964. Compound 2 is of monoclinic system, space group C2/c with a = 17.8712（6）, b = 8.4433（3）, c = 35.5783（13） A, Z = 8, V= 5366.8（3） A3, Dc = 1.360 g.cm-3,/1（MoKa） = 0.980 mm-1, F（000） = 2288, R = 0.0549, wR = 0.1279. In compounds 1 and 2, the central Sn atom is coordinated in a tetradentate manner to assume a distorted tetrahedral configuration. Preliminary biological tests showed that these two compounds have strong acaricidal activity.
文摘Hyoscyamus niger L. （Solanaceae） and Hypericum calycinum L. （Hypericaceae） were collected from two provinces Bursa and Ankara in Turkey during flowering stages. Extracts obtained from H. niger and H. calycinum were tested against two-spotted mite as an acaricidal pesticide. Bioassay experiments were carried out under laboratory conditions using two different methods namely, leaf disc dipping and direct leaf spraying. Two-spotted mite was reared in insectary using Phaseolus vulgaris L. （Fabaceae）. In the leaf dipping method, H. niger extract resulted in an effective adjusted mortality for larvae of 87.13% and for adults of 81.66%. For H. calycinum the adjusted mortality for larvae and adults was 77.77% and 85.97% respectively at the highest concentration. In larval stage, LCs0 values were 5.47 w/v and 3.32 w/v dipping and spraying method respectively. In addition, adult stage, it was determined that LCs0 values were 1.17 w/v and 3.56 w/v in both methods. For leaf spraying method. H. niger resulted in an adjusted showed an effect against larvae and adults mortality rate was 95% and 91% respectively. However, H. calycinum mortality percentage was 80% and 87% at 12% concentration, with LC50 values of 9.62 w/v and 2.13 w/v in dipping method. In this research apart from mortality effects on egg, reproduction was also investigated. Each concentration showed that treated females laid less eggs compared to the untreated females.
文摘Two-spotted spider mite(Tetranychus urticae Koch)(Acari:Tetranychidae)is a very serious pest worldwide,causing considerable damage to vegetables,flowers and fruit crops.The application of plant extracts to control insects can be an effective alternative.In this study,the efficacy of an extract from Juglans regia L.(Juglandecaea)was tested as an alternative to conventional acaricides.Using two different methods,bioassays were used to determine the effects of varying concentrations J.regia(1%,3%,6%,12%)of the extracts.Experiments were carried out using 3 cm diameter leaf disks of Phaseolus vulgaris L.In addition,the effects of the extract on mite reproduction and oviposition were investigated.As a result,in the leaf dipping method,the 12%concentration of the extract caused the highest mortality of nymph(90%)and adult(83.00%)stages.For the spraying method,the mortality of adults at the same concentration was 100%.In bioassays at lower concentrations,the adult mites laid lower numbers of eggs in comparison to the untreated mites.The highest effect occurred at concentration of 12%and the lowest effect was at 1%.The extract was no observed ovicidal effect.
文摘The acaricidal activities of fourteen essential oils and fourteen of their major monoterpenoids were tested against house dust mites Dermatophagoides pteronyssinus. Five concentrations were used over two different time intervals 24 and 48 h under laboratory conditions. In general, it was noticed that the acaricidal effect based on LC50 of either essential oils or monoterpenoids against the mite was time dependant. The LCso values were decreased by increasing of exposure time. Clove, matrecary, chenopodium, rosemary, eucalyptus and caraway oils were shown to have high activity. As for the monoterpenoids, cinnamaldehyde and chlorothymol were found to be the most effective followed by citronellol. This study suggests the use of the essential oils and their major constituents as ecofriendly biodegradable agents for the control of house dust mite, D. pteronyssinus.
基金supported by the National Basic Research Program of China(973 Program,2010CB126104)National Twelfth-five Key Project of China(2011BAE06B01-03)+2 种基金National Natural Science Foundation of China(21272062)the Hunan Provincial Natural Science Foundation (11JJ2006,11JJ3022 and 10JJ3053)Science Plan of Hunan Province (2010FJ2003)
文摘To search for novel 2-arylpyrroles with unique biological activities,a series of novel 2-arylpyrrole derivatives were designed and synthesized,and their structures were characterized by 1 H and 13 C NMR spectroscopy,MS spectrometry,and elemental analysis.Their insecticidal activities against Lepidopteran pests (e.g.Mythimna separata) and acaricidal activities against mites (e.g.Tetranychus urticae) were evaluated.The results of bioassays indicate that some of these title compounds exhibited excellent insecticidal and acaricidal activities.For example,4-bromo-1-((chloromethoxy)methyl)-2-(4-chloro phenyl)-5-(trifluoromethyl)pyrrole-3-carbonitrile (6a),4-bromo-2-(4-chlorophenyl)-1-((2-fluoroethoxy)-methyl)-5-(trifluoromethyl)pyrrole-3-carbonitrile (6d) showed insecticidal activity against Mythimna separata and 4-bromo-2-(4-chlorophenyl)1-((isopropoxymethoxy)methyl)-5-(trifluoro methyl)pyrrole-3-carbonitrile (7d) showed acaricidal activity against Tetranychus urticae.They were more effective than Chlorfenapyr,which has been the only commercialized member of a new class of chemicals of 2-arylpyrroles.
文摘This study compared the control effect of 110 g/L etoxazole SC,15%abamectin·etoxazole SC,30%cyetpyrafen SC,43%bifenazate SC and 1.8%abamectin EC five acaricides sprayed by unmanned aerial vehicle(UAV)on Panonychus citri,aiming to screen out the appropriate acaricide for the control of this pest by UAV spraying.The results showed that 15%abamectin·etoxazole SC and 30%cyetpyrafen SC had the highest control efficacy,which remained above 90%14 d after application.Secondary performance was observed in 43%bifenazate SC and 110 g/L etoxazole SC,which demonstrated enhancing control effect.However,1.8%abamectin EC showed slower effect.Considering the control effect and population reduction rate of P.citri,15%abamectin·etoxazole SC and 30%cyetpyrafen SC were suggested as the effective acaricides for the control of this pest.
基金Supported by Special Fund for the Construction of Modern Agricultural Technology System(NYCYTX-43-syz3)~~
文摘[Objective] This study aimed to compare the effects of sublimed sulfur and acaricide on controlling bee mites.[Method] Three independent experiments were carried out in the same apiary during 2007-2009.[Results] The results showed that this method is effective in eliminating mites settled on combs or hidden during pupae phase,but not in controlling the mites parasitized on adult bees.The control effect on killing Varroa destructor after treated appeared optimal in the first day and then decreased,and that on killing Tvopilaelaps clareae happened in the second day.The results indicated that the effect would be much better if the bees can be treated 1 time to 2 times again in the following 2-5 days after the first treatment.Back to data pool,we found that the treatment of sublimed sulfur paired with acaricide is effective in controlling V.destructor and T.clareae.[Conclusion] This technique could facilitate the apicultural production in the regions suffered from V.destructor and T.clareae.
基金funded by the National Natural Science Foundation of China (No.21202089)the Research Foundation of the Six People Peak of Jiangsu Province (Nos.2011-SWYY-009,2013-SWYY-013)the Technology Project Fund of Nantong City (Nos.AS2013004,CP12013002)
文摘A series of novel pyrazole oxime derivatives containing oxazole ring were designed and synthesized. The title compounds were structurally confirmed by 1H NMR, 13C NMR spectra and elemental analyses. Preliminary bioassay results showed that some of the title compounds displayed promising fungicidal activity besides insecticidal and acaricidal activity. Particularly, compound 8c exhibited potent fungicidal activity against cucumber Pseudoperonospora cubensis beyond good insecticidal activity against Aphis craccivora and Nilaparvata lugens.
基金financial supported by the National Key Research Program of China(No. 2017YFD0200500)National Natural Science Foundation of China(No.21672061)supported by the Fundamental Research Funds for the Central Universities(No. 222201718004)
文摘A novel synthesis of 2,3-dihydroimidazo[1,2-a]pyridin-5 (1H)-one 4 and its derivatives were described.Preliminary bioassays showed that some of the target compounds exhibited excellent insecticidal activities against brown planthopper (Nilaparvata lugens), cowpea aphids (Aphis craccivora) (4,5 a, 5 c, 5 g,5 h, 5 j, 5 r, 6 b, 6 e) and carmine spider mite (Tetranychus cinnabarinus (5 f, 5 s, 6 a) at 500 mg/L. Among them,compound 4 was still active against brown planthopper and cowpea aphids at 4 mg/L. The insecticidal activities were influenced by the types and position of the substituents, which provided guidance for the structure modifications.
基金supported by National Natural Science Foundation of China(No.30700532)the Science and Technology Project of Zhejiang province(Nos.2008C02007-3 and 2008C21029)
文摘Sixteen new derivatives of spiromesifen were synthesized from 3-（2,4,6-trimethylphenyl）-4-hydroxy-△^3-dihydrofuran-2-one. Their bioactivities against diamondbackmoth （Plutella xylostella） and spider mites （Tetranychus cinnabarinus） were also evaluated. The structures of these derivatives were confirmed by ^1H NMR, MS.
文摘Six new 13-O-acylavermectin Bl aglycones(3-8) were synthesized from avermectin B1 aglycone and their bioactivities were evaluated against Spodoptera exigua, Spodoptera eridania, Tetranychus urticae and Aphis fabae.
文摘Groundwater in Egypt plays an important role in the country＇s water budget. South Western desert represent an arid of desert biome within world＇s net-work of the biosphere reserves. In this study, the plant wild vegetation were surveyed in Abu Tartur mining area located in the Southern part of Western depending essentially on the seepage from water line tubes which supplies water demands at Abu Tartur. The vegetation-environment relationships in Abu Tartur are described. Data sets （42 species in 38 plots） beside the pip-line enriched from 13 wells were analyzed, using multivariate procedures, i.e., two-way indicator species analysis （TW1NSPAN）, detrended correspondence analysis （DCA） and detrended canonical correspondence analysis （CCA）, to produce a classification of plant communities in the studied areas and to examine the relationships of that plant communities to certain edaphic factors. Five plant communities were identified. Astragalus vogelii, Chenopodium murale, Citrullus colocynthis, Fagonia arabica, Farsetia aegyptia, Hyoscyamus muticus, Morettia philaeana, Cynodon dactylon, Trichodesma africana, Tarnarix nilotica, Senna italica, Schouwia purpurea, Salsola volkensii and Phragmites australis were common in the study area. Phytochemical survey for nitroprpionic compounds in some taxa belonging to Fabaceae family showed the absence of these toxic compounds by using GC/MS analysis. Also some phytochemical components ofAstragalus vogalii were extracted and identified by GC/MS spectra. A biological activity, in this regard was the screening of methanol extracts for some wild taxa of Abu Tartur against natural Tetranychus urticae as acricidal activity and the methanol extracts of some taxa give high mortality results, like Farsetia aegyptia （86.6%） and Fagonia arabica Burm. f. （70.0%）.
文摘This study reports the extraction of <i>Jatropha curcas</i> leaves using supercritical CO<sub>2</sub>. Experiments were performed varying the pressure (13 and 20 MPa) and the temperature (50°C and 60°C). The model of Sovová for supercritical fluid extraction was fitted to the experimental kinetic extraction curves. Two cell sizes were used and scale up equations compared. GC analysis showed phytol, carvacrol, and hexahydrofarnesyl acetone as major compounds in all the experiments. A maximum yield of 0.95% dry-weight basis was obtained. It was observed a maximum yield (0.95% dry-weight basis) extract obtained at 20 MPa and 50°C. The results indicated that the mass yield increased with the increase of pressure. The bioassays showed that the extract of <i>J. curcas</i> possessed toxicity against <i>Hyalomma lusitanicum</i>.
基金the Research Fund of Shahrekord Uni-versity, Iran
文摘In the present work, the potential of acaricidal activity of chamomile flowers＇ extract was studied against engorged Rhipicephalus annulatus tick under laboratory condition. For this purpose, the engorged females ofRhipicephalus annulatus were exposed to two-fold serial dilutions of chamomile flowers＇ extract （0.5%, 1.0%, 2.0%, 4.0% and 8.0%） using ＂dipping method＂ in vitro. The engorged ticks were immersed in different plant dilutions （five ticks for each dilution） for l rain and they were immediately incubated in separate Petri dishes for each replicate at 26℃ and 80% relative humidity. Mortality rate for each treatment was recorded 5 d after incubation. The mortality rate caused by different dilutions of chamomile flowers＇ extract ranged from 6.67% to 26.7%, whereas no mortality was recorded for non-treated control group. The mass of produced eggs varied from 0.23 g （in 8.0% solution） to 0.58 g （in control）, with no statistical differences between the treatments and control （/＇〉0.05）. Also the chamomile flowers＇ extract in highest concentration used （8.0%） caused 46.67% failure in egg laying in engorged females while no failure was observed for non-treated control group. Macroscopic observations indicated that in effective concentrations of plant （4.0% and 8.0%）, patchy hemorrhagic swelling appeared on the skin of treated ticks. The results presented for the first time in this study imply that chamomile may be considered as a promising plant for biocontrol of cattle fever tick disease in the field condition.
