Kiwifruit bacterial canker is a devastating disease caused by Pseudomonas syringae pv.actinidiae(Psa).NAC transcription factors play a significant role in host immunity.However,the potential molecular mechanism of res...Kiwifruit bacterial canker is a devastating disease caused by Pseudomonas syringae pv.actinidiae(Psa).NAC transcription factors play a significant role in host immunity.However,the potential molecular mechanism of resistance to semi-biotrophic Psa mediated by NAC transcription factors in kiwifruit remains unclear.In this study,we identified a typical NAC transcription factor,AcNAC10,which is involved in the jasmonic acid(JA)pathway and is highly expressed in resistant variety RH12 responsing to Psa.By overexpression and silencing of AcNAC10 in kiwifruit,it plays a positive role in enhancing kiwifruit resistance.Likewise,heterologous expression of AcNAC10 in transgenic Arabidopsis and tomato enhanced resistance to P.syringae.By directly binding to the promoter of AcLOX3,AcNAC10 inhibited its expression as a transcriptional suppressor.Using a yeast one-hybrid screening library,electrophoretic mobility shift assay(EMSA),and dual-luciferase reporter assays,it showed that AcTGA07 can activate the expression of AcNAC10.Moreover,we demonstrated that AcTGA07 decreased JA accumulation independently of the AcNAC10-AcLOX3 pathway.Our study elucidated the transcriptional cascade regulatory network of AcTGA07-AcNAC10-AcLOX3,which enhanced the disease resistance of kiwifruit to Psa by inhibiting JA synthesis.展开更多
基金financially supported by the National Key R&D Program of China(2022YFD1400200)the Natural Science Foundation of China grant 32102174 awarded to W.L.
文摘Kiwifruit bacterial canker is a devastating disease caused by Pseudomonas syringae pv.actinidiae(Psa).NAC transcription factors play a significant role in host immunity.However,the potential molecular mechanism of resistance to semi-biotrophic Psa mediated by NAC transcription factors in kiwifruit remains unclear.In this study,we identified a typical NAC transcription factor,AcNAC10,which is involved in the jasmonic acid(JA)pathway and is highly expressed in resistant variety RH12 responsing to Psa.By overexpression and silencing of AcNAC10 in kiwifruit,it plays a positive role in enhancing kiwifruit resistance.Likewise,heterologous expression of AcNAC10 in transgenic Arabidopsis and tomato enhanced resistance to P.syringae.By directly binding to the promoter of AcLOX3,AcNAC10 inhibited its expression as a transcriptional suppressor.Using a yeast one-hybrid screening library,electrophoretic mobility shift assay(EMSA),and dual-luciferase reporter assays,it showed that AcTGA07 can activate the expression of AcNAC10.Moreover,we demonstrated that AcTGA07 decreased JA accumulation independently of the AcNAC10-AcLOX3 pathway.Our study elucidated the transcriptional cascade regulatory network of AcTGA07-AcNAC10-AcLOX3,which enhanced the disease resistance of kiwifruit to Psa by inhibiting JA synthesis.
