[Objectives] To study the typical HPLC chromatogram of petroleum ether in Abutilon indicum (L.) Sweet from different habitats, in order to provide experimental basis for the quality control of A. indicum and the spect...[Objectives] To study the typical HPLC chromatogram of petroleum ether in Abutilon indicum (L.) Sweet from different habitats, in order to provide experimental basis for the quality control of A. indicum and the spectrum-efficacy relationship of petroleum ether.[Methods] The HPLC chromatogram of petroleum ether was obtained using Agilent SB-C 18 (4.6 mm×250 mm, 5 μm) and acetine-0.1% phosphoric acid solution for gradient elution at temperature of 30 ℃, flow rate of 1.0 mL/min and detection wavelength of 205 nm.[Results] The typical HPLC chromatogram of petroleum ether in A. indicum was preliminarily established, with 12 characteristic peaks. It was verified by A. indicum from different habitats, characterized by reproducibility and stability.[Conclusions] Typical HPLC chromatogram provides a stable new method for the quality control of petroleum ether in A. indicum . This study provides experimental basis for further study on the medicinal parts of A. indicum .展开更多
The relationship between six </span><i><span style="font-family:Verdana;">Abutilon</span></i><span style="font-family:Verdana;"> species was examined using diffe...The relationship between six </span><i><span style="font-family:Verdana;">Abutilon</span></i><span style="font-family:Verdana;"> species was examined using different taxonomic investigation tools. The investigation was carried out using morphological and numerical studies. Fresh materials of </span><i><span style="font-family:Verdana;">Abutilon</span></i><span style="font-family:Verdana;"> species were collected from different localities in Saudi Arabia during 2018 and 2019. Numerical analysis was based on the Principle Coordinates, the Principle Component and the Unweighted Pair Group Method with Arithmetic Algorithm Clustering. The results indicated that there were significant differences based on the morphological characters especially in the leaves, fruits and flowers features. Morphometric studies revealed that the six species of </span><i><span style="font-family:Verdana;">Abutilon</span></i><span style="font-family:Verdana;"> clearly separated in all different analysis.展开更多
[目的]常规玉米田除草模式用药次数多用量大,“一次性除草”是本试验提出的除草新策略,通过多种除草剂的组合探索,获得除草效果最佳的“一次性除草”方案。[方法]玉米种植后待禾本科杂草为3~5叶期,阔叶杂草为4~6叶期,茎叶喷雾处理1次。...[目的]常规玉米田除草模式用药次数多用量大,“一次性除草”是本试验提出的除草新策略,通过多种除草剂的组合探索,获得除草效果最佳的“一次性除草”方案。[方法]玉米种植后待禾本科杂草为3~5叶期,阔叶杂草为4~6叶期,茎叶喷雾处理1次。[结果]30%苯唑草酮悬浮剂33.75 g a.i./hm^(2)+200 g/L氯氟吡氧乙酸异辛酯乳油210 g a.i./hm^(2)+40%砜吡草唑悬浮剂180 g a.i./hm^(2)+JZ-121350 g a.i./hm^(2)与“一封一杀”除草模式相比,“一次性除草”增产9.95%,效果显著。[结论]“一次性除草”技术可有效防控雨养春玉米田一年生杂草,对本试验玉米品种(东单1331)安全;优选出3个除草剂组合为一次性除草提供了可选择的化学防除方案,可根据不同地区雨养玉米田草相、杂草基数、土壤类型、气候特点等,选择适合的除草剂组合大面积示范、推广、应用。展开更多
Objective:To perform a simultaneous quantitative estimation of two biologically active triterpenoid compounds lupeol and a steroid compound,stigmasterol,in Abutilon indicum(A.indicum)using high-performance thin-layer ...Objective:To perform a simultaneous quantitative estimation of two biologically active triterpenoid compounds lupeol and a steroid compound,stigmasterol,in Abutilon indicum(A.indicum)using high-performance thin-layer chromatography(HPTLC).Methods:TLC aluminum plates precoated with silica-gel 60 F254(20 cmí10 cm)were used with a mobile phase of toluene-methanol-formic acid(7.0:2.7:0.3,v/v/v)and densitometric determination of these compounds was carried out at 530 nm in reflectance/absorbance mode.Results:Compact bands for lupeol and stigmasterol were obtained at R_(f)0.52±0.02 and 0.28±0.05.The limit of detection(45 and 18 ng/band),limit of quantification(135 and 54 ng/band),recovery(98.2%-99.7%and 97.2%-99.6%)and precision(≤2.18 and 1.91)were satisfactory for lupeol and stigmasterol respectively.Linearity range for lupeol and stigmasterol were 100-1000(r^(2)=0.9994)and 50-500 ng/band(r^(2)=0.9941)and the contents were estimated as(0.59±0.10)%and(0.83±0.10)%w/w respectively.The total phenolic,flavonoid,proanthocyanidin,alkaloidal and saponin contents of methanolic extract of A.indicum were also measured in this work.According to International Conference on Harmonization(ICH)guidelines,the method was validated for linearity,precision,accuracy,and recovery,limit of detection,limit of quantification,specificity,and robustness.Conclusions:The HPTLC method was found to be reproducible,accurate,and precise and could detect these two compounds at nanogram level from the A.indicum.展开更多
基金Supported by National Natural Science Foundation of China(81260673)
文摘[Objectives] To study the typical HPLC chromatogram of petroleum ether in Abutilon indicum (L.) Sweet from different habitats, in order to provide experimental basis for the quality control of A. indicum and the spectrum-efficacy relationship of petroleum ether.[Methods] The HPLC chromatogram of petroleum ether was obtained using Agilent SB-C 18 (4.6 mm×250 mm, 5 μm) and acetine-0.1% phosphoric acid solution for gradient elution at temperature of 30 ℃, flow rate of 1.0 mL/min and detection wavelength of 205 nm.[Results] The typical HPLC chromatogram of petroleum ether in A. indicum was preliminarily established, with 12 characteristic peaks. It was verified by A. indicum from different habitats, characterized by reproducibility and stability.[Conclusions] Typical HPLC chromatogram provides a stable new method for the quality control of petroleum ether in A. indicum . This study provides experimental basis for further study on the medicinal parts of A. indicum .
文摘The relationship between six </span><i><span style="font-family:Verdana;">Abutilon</span></i><span style="font-family:Verdana;"> species was examined using different taxonomic investigation tools. The investigation was carried out using morphological and numerical studies. Fresh materials of </span><i><span style="font-family:Verdana;">Abutilon</span></i><span style="font-family:Verdana;"> species were collected from different localities in Saudi Arabia during 2018 and 2019. Numerical analysis was based on the Principle Coordinates, the Principle Component and the Unweighted Pair Group Method with Arithmetic Algorithm Clustering. The results indicated that there were significant differences based on the morphological characters especially in the leaves, fruits and flowers features. Morphometric studies revealed that the six species of </span><i><span style="font-family:Verdana;">Abutilon</span></i><span style="font-family:Verdana;"> clearly separated in all different analysis.
文摘[目的]常规玉米田除草模式用药次数多用量大,“一次性除草”是本试验提出的除草新策略,通过多种除草剂的组合探索,获得除草效果最佳的“一次性除草”方案。[方法]玉米种植后待禾本科杂草为3~5叶期,阔叶杂草为4~6叶期,茎叶喷雾处理1次。[结果]30%苯唑草酮悬浮剂33.75 g a.i./hm^(2)+200 g/L氯氟吡氧乙酸异辛酯乳油210 g a.i./hm^(2)+40%砜吡草唑悬浮剂180 g a.i./hm^(2)+JZ-121350 g a.i./hm^(2)与“一封一杀”除草模式相比,“一次性除草”增产9.95%,效果显著。[结论]“一次性除草”技术可有效防控雨养春玉米田一年生杂草,对本试验玉米品种(东单1331)安全;优选出3个除草剂组合为一次性除草提供了可选择的化学防除方案,可根据不同地区雨养玉米田草相、杂草基数、土壤类型、气候特点等,选择适合的除草剂组合大面积示范、推广、应用。
文摘Objective:To perform a simultaneous quantitative estimation of two biologically active triterpenoid compounds lupeol and a steroid compound,stigmasterol,in Abutilon indicum(A.indicum)using high-performance thin-layer chromatography(HPTLC).Methods:TLC aluminum plates precoated with silica-gel 60 F254(20 cmí10 cm)were used with a mobile phase of toluene-methanol-formic acid(7.0:2.7:0.3,v/v/v)and densitometric determination of these compounds was carried out at 530 nm in reflectance/absorbance mode.Results:Compact bands for lupeol and stigmasterol were obtained at R_(f)0.52±0.02 and 0.28±0.05.The limit of detection(45 and 18 ng/band),limit of quantification(135 and 54 ng/band),recovery(98.2%-99.7%and 97.2%-99.6%)and precision(≤2.18 and 1.91)were satisfactory for lupeol and stigmasterol respectively.Linearity range for lupeol and stigmasterol were 100-1000(r^(2)=0.9994)and 50-500 ng/band(r^(2)=0.9941)and the contents were estimated as(0.59±0.10)%and(0.83±0.10)%w/w respectively.The total phenolic,flavonoid,proanthocyanidin,alkaloidal and saponin contents of methanolic extract of A.indicum were also measured in this work.According to International Conference on Harmonization(ICH)guidelines,the method was validated for linearity,precision,accuracy,and recovery,limit of detection,limit of quantification,specificity,and robustness.Conclusions:The HPTLC method was found to be reproducible,accurate,and precise and could detect these two compounds at nanogram level from the A.indicum.
