Pseudomonas aeruginosa is one of the leading nosocomial pathogens worldwide, and their infections are difficult to treat due to acquired resistance to many antibiotics. This study aimed to detect class 1 and 2 integro...Pseudomonas aeruginosa is one of the leading nosocomial pathogens worldwide, and their infections are difficult to treat due to acquired resistance to many antibiotics. This study aimed to detect class 1 and 2 integrons and antibiotic susceptibility of clinical isolates of P. aeruginosa. Two hundred and five P. aeruginosa strains were collected from the seven general state hospitals in Turkey. They were characterized by antimicrobial susceptibility testing, screened for class 1 and class 2 integrons, and evaluated for the association between antibiotic resistance phenotypes and the presence of integrons. intI gene was amplified in 10 isolates (4.87%) by PCR and in seven isolates of them (70%) were found different gene cassettes. The aadA gene integrated into the class 1 integrons was most frequently found and it was followed by aac genes and blaOXA family genes. Sequence analysis of variable regions of the class 1 integrons showed five gene cassette arrays;aadA1(99%), aac(3)-Id(82%)-orf-aac(3”)-Ia(99%), aac(3)-Ie(83%)-blaoxa10(100%)- aadA1 (100%), aadA6(99%, 100%), aac(6’)-I(97%)-orf-aadA2(99%). No class 2 integron was detected. This study is the first multicenter study for class 1 integrons and it indicates the low rate of presence of class 1 gene cassette in P. aeruginosa.展开更多
Plastid transformation offers valuable benefits in plant biotechnology,such as high-level transgene expression and the absence of gene silencing.Here we describe the first protocol of a plastid transformation system f...Plastid transformation offers valuable benefits in plant biotechnology,such as high-level transgene expression and the absence of gene silencing.Here we describe the first protocol of a plastid transformation system for a woody vine(liana)kiwifruit(Actinidia chinensis).The transgenic DNA carries a spectinomycin-resistance gene(aadA)cassette and a green fluorescent protein(GFP)reporter gene cassette,flanked by two adjacent kiwifruit plastid genome sequences,thereby allowing targeted insertion between the trnfM and trnG genes.Six spectinomycin-resistant shoots were obtained out of 12 plates subjected to bombardment,and two were positive events,confirmed through PCR and Southern blot analyses.The GFP was localized to plastids as monitored by confocal laser scanning microscopy and reached 2.5%of leaf total soluble protein.Success in kiwifruit extends transplastomic technology of woody species beyond poplar,and will provide an attractive biosynthetic chassis for molecular farming.展开更多
基金supported by Recep Tayyip Erdogan University Research Fund Grants BAP-2013.102.03.12 and BAP-2013.102.03.13.
文摘Pseudomonas aeruginosa is one of the leading nosocomial pathogens worldwide, and their infections are difficult to treat due to acquired resistance to many antibiotics. This study aimed to detect class 1 and 2 integrons and antibiotic susceptibility of clinical isolates of P. aeruginosa. Two hundred and five P. aeruginosa strains were collected from the seven general state hospitals in Turkey. They were characterized by antimicrobial susceptibility testing, screened for class 1 and class 2 integrons, and evaluated for the association between antibiotic resistance phenotypes and the presence of integrons. intI gene was amplified in 10 isolates (4.87%) by PCR and in seven isolates of them (70%) were found different gene cassettes. The aadA gene integrated into the class 1 integrons was most frequently found and it was followed by aac genes and blaOXA family genes. Sequence analysis of variable regions of the class 1 integrons showed five gene cassette arrays;aadA1(99%), aac(3)-Id(82%)-orf-aac(3”)-Ia(99%), aac(3)-Ie(83%)-blaoxa10(100%)- aadA1 (100%), aadA6(99%, 100%), aac(6’)-I(97%)-orf-aadA2(99%). No class 2 integron was detected. This study is the first multicenter study for class 1 integrons and it indicates the low rate of presence of class 1 gene cassette in P. aeruginosa.
基金supported by grants from the National Natural Science Foundation of China(32271912,32071477).
文摘Plastid transformation offers valuable benefits in plant biotechnology,such as high-level transgene expression and the absence of gene silencing.Here we describe the first protocol of a plastid transformation system for a woody vine(liana)kiwifruit(Actinidia chinensis).The transgenic DNA carries a spectinomycin-resistance gene(aadA)cassette and a green fluorescent protein(GFP)reporter gene cassette,flanked by two adjacent kiwifruit plastid genome sequences,thereby allowing targeted insertion between the trnfM and trnG genes.Six spectinomycin-resistant shoots were obtained out of 12 plates subjected to bombardment,and two were positive events,confirmed through PCR and Southern blot analyses.The GFP was localized to plastids as monitored by confocal laser scanning microscopy and reached 2.5%of leaf total soluble protein.Success in kiwifruit extends transplastomic technology of woody species beyond poplar,and will provide an attractive biosynthetic chassis for molecular farming.
