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当归芍药散调控EPO/PPAR-γ/AXL通路增强小胶质细胞吞噬改善血管性认知障碍
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作者 李翔 袁宁宁 +2 位作者 徐莹 苏越 成小兰 《中国中西医结合杂志》 北大核心 2025年第6期706-713,共8页
目的 观察当归芍药散(DSS)对血管性认知障碍(VCI)大鼠认知功能和促红细胞生成素(EPO)/过氧化物酶体增殖物激活受体γ(PPAR-γ)/AXL受体酪氨酸激酶(AXL)/AXL通路的影响,探讨其作用机制。方法 将40只SD大鼠随机分为假手术组(Sham组)、模型... 目的 观察当归芍药散(DSS)对血管性认知障碍(VCI)大鼠认知功能和促红细胞生成素(EPO)/过氧化物酶体增殖物激活受体γ(PPAR-γ)/AXL受体酪氨酸激酶(AXL)/AXL通路的影响,探讨其作用机制。方法 将40只SD大鼠随机分为假手术组(Sham组)、模型组(VCI组)、模型+当归芍药散低剂量组(5 g/kg)、模型+当归芍药散高剂量组(10 g/kg)及模型+尼莫地平组(Nimodipine,NMP组,9.45 mg/kg),每组8只。除Sham组外,其余各组采用双侧颈总动永久性脉结扎法建立VCI模型,造模后立即给药干预,Sham组和模型组灌胃等量生理盐水,给药4周。采用Morris水迷宫测试大鼠学习记忆能力,HE染色检测大鼠海马组织损伤,牢固蓝染色(LFB)检测大鼠海马髓鞘损伤,免疫荧光染色检测大鼠小胶质细胞活化状态和髓鞘相关蛋白,荧光定量PCR检测小胶质细胞M2极化标志物和吞噬相关基因mRNA表达水平,ELISA检测炎症因子表达,Western Blot检测EPO通路相关蛋白表达。结果 与模型组比较,DSS高剂量组穿越平台次数增加,目标象限停留时间延长,逃逸潜伏期缩短(P<0.01)。ELISA检测结果显示,DSS高剂量组大鼠海马中炎症相关因子IL-6、TNF-α、单核细胞趋化蛋白1(MCP-1)降低(P<0.05)。荧光定量PCR结果显示,DSS高剂量组小胶质细胞M1极化标志物CD68表达下降,而M2极化标志物CD206、精氨酸酶1(Arg-1)表达升高(P<0.01,P<0.05)。Western Blot结果表明,DSS组EPO、PPAR-γ和AXL蛋白表达上调(P<0.01)。结论 DSS高剂量通过调控EPO/PPAR-γ/AXL通路增强海马小胶质细胞吞噬功能,并向M2型极化减轻神经炎症,进而改善VCI。 展开更多
关键词 血管性认知障碍 当归芍药散 小胶质细胞 促红细胞生成素 过氧化物酶体增殖物激活受体Γ axl受体酪氨酸激酶 中药复方 中西医结合
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益母草碱调节GAS6/Axl信号通路对冠心病大鼠心肌损伤的影响 被引量:2
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作者 孟王桃 崔冬玲 +2 位作者 吴冬景 陈超 张颖颖 《中国药房》 北大核心 2025年第1期51-56,共6页
目的探讨益母草碱对生长停滞特异性蛋白6(GAS6)/酪氨酸蛋白激酶受体(Axl)信号通路的影响,阐明其减轻冠心病大鼠心肌损伤的机制。方法构建冠心病大鼠模型,将造模成功的大鼠按随机抽样方法分为模型组,益母草碱低、高剂量组(分别灌胃25、10... 目的探讨益母草碱对生长停滞特异性蛋白6(GAS6)/酪氨酸蛋白激酶受体(Axl)信号通路的影响,阐明其减轻冠心病大鼠心肌损伤的机制。方法构建冠心病大鼠模型,将造模成功的大鼠按随机抽样方法分为模型组,益母草碱低、高剂量组(分别灌胃25、100 mg/kg益母草碱+腹腔注射75 mg/kg生理盐水),益母草碱高剂量+GAS6/Axl信号通路抑制剂组(灌胃100 mg/kg益母草碱+腹腔注射75 mg/kg的R428),每组12只;另取12只正常大鼠作对照组。各给药组大鼠给予相应药物,对照组和模型组大鼠灌胃并腹腔注射等体积生理盐水,每天1次,连续48 d。给药结束后,检测大鼠心功能和血清中炎症因子及心肌损伤标志物水平;观察其心肌组织病理形态;检测其心肌组织细胞凋亡率和心肌组织中凋亡及GAS6/Axl信号通路相关蛋白表达。结果与对照组比较,模型组大鼠出现心肌细胞与心肌纤维排列紊乱、心肌细胞肥大、细胞核固缩等病变,左室射血分数、左室短轴缩短率、二尖瓣环舒张早期与舒张晚期运动速度之比及GAS6蛋白相对表达量和B细胞淋巴瘤2/B细胞淋巴瘤2相关X蛋白、磷酸化Axl/Axl比值均显著降低(P<0.05),肿瘤坏死因子α、白细胞介素1β、白细胞介素6、肌酸激酶同工酶、肌钙蛋白Ⅰ、肌红蛋白水平以及心肌组织细胞凋亡率和剪切型胱天蛋白酶3/胱天蛋白酶3比值均显著升高(P<0.05);益母草碱各剂量组大鼠上述病理情况及各检测指标均显著改善(P<0.05),且益母草碱高剂量组效果较益母草碱低剂量组明显(P<0.05);R428处理可逆转高剂量益母草碱对冠心病大鼠心肌损伤的改善作用(P<0.05)。结论益母草碱可减轻冠心病大鼠心肌损伤,其作用机制可能与激活GAS6/Axl信号通路相关。 展开更多
关键词 益母草碱 GAS6/axl信号通路 冠心病 心肌损伤
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Dynamic modeling and simulation of irregular faults in axle box bearings of high-speed trains
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作者 Xiaohui GU Yajia WANG +2 位作者 Pengfei LIU Zechao LIU Shaopu YANG 《Applied Mathematics and Mechanics(English Edition)》 2025年第10期1883-1902,共20页
Axle box bearings are critical components of high-speed trains.Localized defects,such as pitting and spalling,on raceways or rollers pose significant threats to the operational safety of railway vehicles.In this work,... Axle box bearings are critical components of high-speed trains.Localized defects,such as pitting and spalling,on raceways or rollers pose significant threats to the operational safety of railway vehicles.In this work,a novel bearing-flexible axle boxvehicle coupling model is established to explore the vibration characteristics of axle box bearings with irregular localized defects.First,based on the contact and kinematic relationship between rollers and raceways,the three-dimensional(3D)bearing force elements are analyzed and formulated.Second,the established model and a flexible axle box are integrated into the vehicle,and the responses of the normal and faulty bearings under the combined excitations of wheel roughness and track irregularities are simulated.Third,the simulation results are verified through a rolling-vibrating test bench for full-scale wheelsets of high-speed trains.The comparisons of the fault-induced repetitive transients in the time-domain and the fault characteristic frequencies in the envelope spectra demonstrate the efficiency of the proposed model.Finally,based on the flexible axle box model,a sensitivity analysis of the accelerometer placements to the bearing faults is carried out,and the optimal one is identified based on both the time-domain and frequency-domain signal-to-noise ratios(SNRs)for engineering applications. 展开更多
关键词 axle box bearing irregular fault track irregularity flexible axle box accelerometer placement selection
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当归六黄汤通过激活Tyro3/Axl受体活性治疗桥本甲状腺炎大鼠的实验研究 被引量:1
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作者 吴静泽 王晔恺 +1 位作者 陈位 金丹雯 《浙江中西医结合杂志》 2025年第3期200-204,共5页
目的探讨当归六黄汤治疗桥本甲状腺炎大鼠的可能作用机制。方法24只雄性大鼠适应性喂养1周后,按随机数字表法分为空白组、模型组、安慰剂组及当归六黄汤组,每组6只。空白组不予任何处理直接处死。模型组、安慰剂组和当归六黄汤组大鼠通... 目的探讨当归六黄汤治疗桥本甲状腺炎大鼠的可能作用机制。方法24只雄性大鼠适应性喂养1周后,按随机数字表法分为空白组、模型组、安慰剂组及当归六黄汤组,每组6只。空白组不予任何处理直接处死。模型组、安慰剂组和当归六黄汤组大鼠通过在尾根和足垫皮下注射甲状腺球蛋白各100μg,每周1次,持续7周,建立桥本甲状腺炎模型。从第8周开始,安慰剂组和当归六黄汤组分别予安慰剂(40 g/L)和当归六黄汤水煎液(144 g/L)灌胃,每次2 mL,每天1次。第11周处死模型组、安慰剂组和中药组所有大鼠,采集血液和甲状腺组织。CT检测大鼠甲状腺组织肿胀情况,流式细胞术检测外周血CD3^(+)T细胞表面Tyro3、Axl的平均荧光强度(MFI),酶联免疫吸附试验(ELISA)检测血清抗甲状腺球蛋白抗体(TG-Ab)和抗甲状腺过氧化物酶抗体(TPO-Ab)水平,免疫组化检测甲状腺组织Tyro3和Axl表达,实时荧光定量PCR检测甲状腺组织Tyro3、Axl、B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)mRNA表达。结果与模型组和安慰剂组比较,中药组CT下可见甲状腺组织炎性减退,边界逐渐清晰。与空白组比较,模型组血清TG-Ab[(79.77±6.06)U/mL比(43.00±9.36)U/mL,P<0.01]、TPO-Ab[(8.04±0.99)U/mL比(2.76±1.11)U/mL,P<0.01]及甲状腺组织Bax mRNA[(1.39±0.09)比(0.27±0.11),P<0.01]表达均升高,甲状腺组织Bcl-2 mRNA[(1.29±0.14)比(3.39±0.37),P<0.01]表达降低。与模型组和安慰剂组比较,当归六黄汤组血清TG-Ab[(43.69±11.51)U/mL比(79.77±6.06)U/mL、(82.17±12.11)U/mL,P<0.01]、TPO-Ab[(3.02±0.73)U/mL比(8.04±0.99)U/mL、(7.84±0.79)U/mL,P<0.01]及甲状腺组织Bax mRNA[(0.24±0.10)比(1.39±0.09)、(1.38±0.34),P<0.01]表达均降低,甲状腺组织Bcl-2 mRNA表达升高[(2.89±0.32)比(1.29±0.14)、(1.36±0.29),P<0.01]。与空白组比较,模型组外周血CD3^(+)T细胞Tyro3、Axl MFI[Tyro3:(1325.53±129.45)比(1775.95±120.73),Axl:(130.10±11.71)比(156.58±9.27),P<0.01]及甲状腺组织Tyro3、Axl mRNA[Tyro3:(0.67±0.13)比(3.06±0.23),Axl:(0.14±0.05)比(0.71±0.17),P<0.01]和平均光密度(AOD)[Tyro3:(119.45±14.47)比(266.20±33.21),Axl:(117.96±13.06)比(240.06±21.50),P<0.01]均降低。与模型组和安慰剂组比较,当归六黄汤组外周血CD3^(+)T细胞Tyro3、Axl MFI[Tyro3:(1769.92±151.90)比(1325.53±129.45)、(1241.57±193.40),Axl:(150.08±15.32)比(130.10±11.71)、(127.50±9.94),P<0.01]及甲状腺组织Tyro3、Axl mRNA[Tyro3:(2.69±0.44)比(0.67±0.13)、(0.71±0.33),Axl:(0.62±0.10)比(0.14±0.05)、(0.12±0.04),P<0.01]和AOD[Tyro3:(251.41±32.28)比(119.45±14.47)、(115.69±24.81),Axl:(223.23±19.12)比(117.96±13.06)、(119.69±20.48),P<0.01]均升高。结论当归六黄汤对桥本甲状腺炎大鼠的抑制效果可能和激活Tyro3/Axl受体信号通路进而引起凋亡抑制有关。 展开更多
关键词 大鼠 桥本甲状腺炎 当归六黄汤 Tyro3/axl受体 B细胞淋巴瘤-2 B细胞淋巴瘤-2相关X蛋白
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金合欢素调节GAS6/Axl信号通路减轻慢性心力衰竭大鼠心肌损伤
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作者 郝雨辉 贾亿卿 张志刚 《现代药物与临床》 2025年第10期2420-2425,共6页
目的探究金合欢素调节生长停滞特异性蛋白6(GAS6)/Axl受体酪氨酸激酶(Axl)信号通路对慢性心力衰竭大鼠心肌损伤的影响。方法采用ip阿霉素的方法构建慢性心力衰竭大鼠模型,将大鼠随机分为对照组、模型组、金合欢素(5、10、20 mg/kg)组、... 目的探究金合欢素调节生长停滞特异性蛋白6(GAS6)/Axl受体酪氨酸激酶(Axl)信号通路对慢性心力衰竭大鼠心肌损伤的影响。方法采用ip阿霉素的方法构建慢性心力衰竭大鼠模型,将大鼠随机分为对照组、模型组、金合欢素(5、10、20 mg/kg)组、卡托普利(100 mg/kg)组、金合欢素+R428(20 mg/kg金合欢素+75 mg/kg GAS6/Axl信号通路抑制剂R428)组,每组12只。心脏超声检测大鼠心功能指标;生化仪检测大鼠心肌损伤指标;ELISA检测大鼠心力衰竭指标;HE染色观察心肌组织病理变化;TUNEL检测心肌组织细胞凋亡;Western blotting检测GAS6/Axl信号通路相关蛋白表达。结果金合欢素各剂量组、卡托普利组较模型组心肌细胞损伤程度依次得到改善;相较于金合欢素20 mg/kg组,金合欢素+R428组心肌细胞损伤程度有所加剧。相比于模型组,金合欢素各剂量组、卡托普利组FS、LVEF、GAS6蛋白表达、p-Axl/Axl值升高,LVDs、LVDd、c TnT、cTnI、CK-MB、NT-proBNP水平,心肌细胞凋亡率降低(P<0.05);相比于金合欢素20 mg/kg组,金合欢素+R428组FS、LVEF、GAS6蛋白表达及p-Axl/Axl值降低,LVDs、LVDd、c TnT、cTnI、CKMB、NT-proBNP水平及心肌细胞凋亡率升高(P<0.05)。结论金合欢素可能通过激活GAS6/Axl信号通路减轻慢性心力衰竭大鼠心肌损伤。 展开更多
关键词 金合欢素 慢性心力衰竭 生长停滞特异性蛋白6 axl受体酪氨酸激酶 心肌损伤
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A clip-on composite sensor based packaging design method for fiber Bragg grating axle counter
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作者 Mengyao Zhao Xueyun Cao +2 位作者 Longsheng Wang Yang Peng Tao Wang 《Railway Sciences》 2025年第5期647-665,共19页
Purpose–To address the encapsulation challenge of fiber Bragg grating(FBG)sensors in complex railway environments,this paper designs a clip-on composite sensor enabling installation-friendly deployment and long-term ... Purpose–To address the encapsulation challenge of fiber Bragg grating(FBG)sensors in complex railway environments,this paper designs a clip-on composite sensor enabling installation-friendly deployment and long-term axle counting system monitoring.Design/methodology/approach–Wheel–rail mechanical behavior was simulated via finite element analysis(FEA)to determine optimal sensor placement.A clip-on composite sensor was subsequently engineered.Stress transduction efficacy was validated through FEA quantification of stress responses at the axle counter location.Findings–The proposed FBG axle counter integrates temperature compensation and anti-detachment monitoring as well as advantages such as simplified installation with minimal maintenance and sustained operational reliability.It effectively transmits stress,yielding a measured strain of 39μe under static loading conditions without sensitivity-enhancing elements.Originality/value–This study performs FEA of wheel-rail stress distribution and engineers the dual-slot composite sensor,FEAwas conducted to quantify the stress magnitude at the axle sensor position of the dual-slot composite sensor.Additionally,FEA was performed on sensors with different structural configurations,including adjustments to the axle sensor position,number of slots and axle position.The results confirmed that the designed composite sensor exhibits superior stress transfer characteristics. 展开更多
关键词 Fiber Bragg grating axle counting Finite element Sensor Wheel-rail forces
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Damage Tolerance Assessment of Heavy-Duty Freight Railway Axles with Artificial Defects
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作者 Hai Zhao Ding Liao +4 位作者 Jiewei Gao Shunpeng Zhu Ligen Liu Jing Han Shantong Li 《Chinese Journal of Mechanical Engineering》 2025年第2期394-406,共13页
Heavy-duty freight railway axles are no less important than those of passenger trains,owing to the potentially catastrophic results caused by the derailment of trains carrying hazardous substances.Intrinsic and extrin... Heavy-duty freight railway axles are no less important than those of passenger trains,owing to the potentially catastrophic results caused by the derailment of trains carrying hazardous substances.Intrinsic and extrinsic imperfections challenge classical design theories built based on the safe life concept,and damage tolerance assessment becomes vital for the safety and reliability of long-term serviced railway axles,as pits and scratches are common defects for heavy-duty railway axles.In this work,four-point rotating bending fatigue tests of AAR-CM railway axle steel specimens with semicircular and circumferential groove notches are conducted.The fatigue limit of the semicircular notched specimens was evaluated based on fracture mechanics theory,in which non-conservative results are obtained by the El Haddad model and the S–N curves of circumferential groove notched specimens are correlated by the theory of critical distance(TCD). 展开更多
关键词 Heavy-duty railway axle Damage tolerance NOTCH K-T diagram Theory of critical distance
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Lightweight Design of Front Axle Structure of a Certain Type of Automobile
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作者 Chuan qi LI Jian ZOU +2 位作者 Bing Ll Yiming LEI Mingshuo ZHANG 《Mechanical Engineering Science》 2025年第1期26-32,共7页
In this paper,the front axle of a certain model is taken as the research object,and the stress and deformation of the frontaxle under three typical working conditions are analyzed by finite element technology.Based on... In this paper,the front axle of a certain model is taken as the research object,and the stress and deformation of the frontaxle under three typical working conditions are analyzed by finite element technology.Based on the simulation results,the 3D model of the front axle was optimized,and the finite element analysis of the optimized structure of the front axle under three typical working conditions was carried out to verify the correctness of the model.Finally,the fatigue tool module of ANSYS Workbench was used to analyze the fatigue life of the front axle under the optimized emergency conditions,and the feasibility of the model was verified.The analysis data shows that the design of the front axle components still has a lot of potential for lightweighting,and the weight of the front axle can be reduced by 6.73%through optimization,and the performance of the front axle can also meet the needs of use.The research conclusionhas a certain reference value for the lightweight design of automobile front axle. 展开更多
关键词 automobile front axle structural optimization Fatigue life
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甲基莲心碱调节Gas6/Axl信号通路对带状疱疹后遗神经痛小鼠疼痛反应的影响
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作者 雷剑 黄丹 葛兴森 《河北医学》 2025年第11期1801-1808,共8页
目的:探究甲基莲心碱(NEF)调节生长停滞特异性蛋白6(Gas6)/酪氨酸蛋白激酶受体(Axl)信号通路对带状疱疹后遗神经痛(PHN)小鼠疼痛反应的影响。方法:构建PHN小鼠模型。建模成功的小鼠随机分成PHN组、NEF-L组(5mg/kg)、NEF-M组(10mg/kg)、N... 目的:探究甲基莲心碱(NEF)调节生长停滞特异性蛋白6(Gas6)/酪氨酸蛋白激酶受体(Axl)信号通路对带状疱疹后遗神经痛(PHN)小鼠疼痛反应的影响。方法:构建PHN小鼠模型。建模成功的小鼠随机分成PHN组、NEF-L组(5mg/kg)、NEF-M组(10mg/kg)、NEF-H组(20mg/kg)、R428组(20mg/kg和125mg/kg Gas6/Axl信号通路抑制剂R428),NEF和R428均通过腹腔注射给药,每组各10只。另选取10只正常C57BL/6J小鼠作为对照组(NC组)。检测各组小鼠痛阈值及自发缩足反射次数;HE染色观察脊髓组织病理变化;ELISA检测小鼠脊髓组织炎症因子、前列腺素E2(PGE2)、5-羟色胺(5-HT)、P物质(SP)表达水平;TUNEL观察小鼠脊髓神经细胞凋亡;六胺银染色观察小鼠脊髓组织形态学改变;Western blot检测小鼠脊髓组织Gas6/Axl信号通路及凋亡相关蛋白表达。结果:PHN组小鼠脊髓组织及神经元纤维结构损伤严重,自发缩足反射次数、IL-6、IL-1β、PGE2、5-HT、SP、神经元凋亡率、Bax表达显著高于NC组(P<0.05),机械痛阈值、热痛阈值、Gas6、p-Axl/Axl、Bcl-2显著低于NC组(P<0.05);NEF-L组、NEF-M组、NEF-H组小鼠脊髓组织及神经元纤维结构损伤减轻,自发缩足反射次数、IL-6、IL-1β、PGE2、5-HT、SP、神经元凋亡率、Bax表达低于PHN组(P<0.05),机械痛阈值、热痛阈值、Gas6、p-Axl/Axl、Bcl-2高于PHN组(P<0.05);R428组小鼠脊髓组织及神经元纤维结构损伤加重,自发缩足反射次数、IL-6、IL-1β、PGE2、5-HT、SP、神经元凋亡率、Bax表达显著高于NEF-H组(P<0.05),机械痛阈值、热痛阈值、Gas6、p-Axl/Axl、Bcl-2显著低于NEF-H组(P<0.05)。结论:NEF能够改善PHN小鼠疼痛以及炎症反应,可能与激活Gas6/Axl信号通路密切相关。 展开更多
关键词 带状疱疹后遗神经痛 甲基莲心碱 生长停滞特异性蛋白6/酪氨酸蛋白激酶受体信号通路 疼痛反应
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受体酪氨酸激酶AXL在肿瘤生物学中的作用研究进展 被引量:2
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作者 何凯桐 周阳 +2 位作者 王明珠 郭婷 孙轶华 《现代肿瘤医学》 CAS 2024年第1期167-171,共5页
AXL是一种受体酪氨酸激酶(receptor tyrosine kinase,RTK),属于TAM(TYRO3-AXL-MER)家族。在生理条件下,AXL表达于人体的心脏、骨骼肌、甲状腺中。AXL通过调控多条信号通路介导了肿瘤细胞的血管生成、迁移、侵袭、增殖与转移等过程。此外... AXL是一种受体酪氨酸激酶(receptor tyrosine kinase,RTK),属于TAM(TYRO3-AXL-MER)家族。在生理条件下,AXL表达于人体的心脏、骨骼肌、甲状腺中。AXL通过调控多条信号通路介导了肿瘤细胞的血管生成、迁移、侵袭、增殖与转移等过程。此外,AXL在多种癌症中高表达,对于肿瘤患者的预后评价具有重要意义,并极有可能成为一种新的诊断及预后生物标志物和治疗靶点。本文就近年来AXL在肿瘤生物学中的作用研究进展进行综述。 展开更多
关键词 受体酪氨酸激酶 axl 肿瘤 耐药
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血清Occludin、Axl水平与急性缺血性脑卒中溶栓后出血转化的关系 被引量:6
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作者 张立娜 王海虹 王适达 《国际检验医学杂志》 CAS 2024年第2期246-249,共4页
目的探讨血清闭合蛋白(Occludin)、Axl水平与急性缺血性脑卒中(AIS)溶栓治疗后出血转化(HT)的关系。方法选取2019年1月至2022年1月该院AIS患者126例为研究对象,根据是否发生HT分为HT组(43例)和非HT组(83例)。比较两组一般资料及血清Occl... 目的探讨血清闭合蛋白(Occludin)、Axl水平与急性缺血性脑卒中(AIS)溶栓治疗后出血转化(HT)的关系。方法选取2019年1月至2022年1月该院AIS患者126例为研究对象,根据是否发生HT分为HT组(43例)和非HT组(83例)。比较两组一般资料及血清Occludin、Axl水平,采用多因素Logistic回归分析AIS患者发生HT的影响因素,采用受试者工作特征(ROC)曲线分析血清Occludin、Axl对AIS患者发生HT的预测价值,并分析血清Occludin、Axl预测HT发生的交互作用。结果两组心房颤动、入院时美国国立卫生研究院卒中量表(NIHSS)评分及血清Occludin、Axl水平比较,差异有统计学意义(P<0.05);多因素Logistic回归分析显示,心房颤动、入院时NIHSS评分高、血清Occludin高水平、Axl低水平均为AIS患者发生HT的独立危险因素(P<0.05);ROC曲线结果显示,血清Occludin、Axl预测AIS患者溶栓治疗后HT风险均有较高价值,二者联合预测的曲线下面积(AUC)、灵敏度、特异度分别为0.742、70.73%、66.93%。Occludin和Axl对HT发生存在负向交互作用。结论AIS患者血清Occludin、Axl均异常表达,可作为HT的预测指标,且二者对HT发生存在负向交互作用。 展开更多
关键词 闭合蛋白 axl 急性缺血性脑卒中 出血转化 交互作用
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可溶性Axl受体酪氨酸激酶在老年急性失代偿性心力衰竭患者短期预后中的价值
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作者 马同亮 韩若东 +1 位作者 黄羽 朱德才 《中华老年心脑血管病杂志》 CAS 北大核心 2024年第3期262-266,共5页
目的 探讨急性失代偿性心力衰竭(ADHF)患者血清可溶性Axl受体酪氨酸激酶(sAxlTK)水平在评估短期预后中的临床价值。方法 选择2018年1月1日至2021年10月1日亳州市人民医院收治的老年ADHF患者238例,根据随访90 d内复合事件发生情况分为预... 目的 探讨急性失代偿性心力衰竭(ADHF)患者血清可溶性Axl受体酪氨酸激酶(sAxlTK)水平在评估短期预后中的临床价值。方法 选择2018年1月1日至2021年10月1日亳州市人民医院收治的老年ADHF患者238例,根据随访90 d内复合事件发生情况分为预后不良组45例和预后良好组193例。又根据血清sAxlTK最佳截断值分为高水平组80例和低水平组158例。检测血清sAxlTK、肌钙蛋白I(cTnI)和N末端B型钠尿肽前体(NT-proBNP)水平。结果 预后不良组血清sAxlTK水平明显高于预后良好组[43.89(33.95,51.44)μg/L vs 23.89(18.73,33.92)μg/L,P<0.01]。多因素logistic回归分析显示,血清cTnI和sAxlTK是ADHF患者短期预后不良的独立危险因素(OR=1.922,95%CI:1.035~3.568,P=0.039;OR=1.021,95%CI:1.008~1.034,P=0.001)。ROC曲线分析显示,血清sAxlTK、cTnI和NT-proBNP联合预测短期预后不良的曲线下面积为0.836(95%CI:0.778~0.895)。高水平组90 d内复合事件发生率明显高于低水平组(45.0%vs 5.7%,P<0.05)。Kaplan-Meier曲线分析显示,高水平组复合事件累积发生率较低水平组更高(χ^(2)=66.991,P_(log rank)<0.01)。高水平组总生存率较低水平组更低,生存预后更差(χ^(2)=16.899,P_(log rank)<0.01)。结论 老年ADHF患者入院时高血清sAxlTK水平与短期预后不良高风险有关。血清sAxlTK有希望成为短期预后不良早期预测的有用工具,且其与cTnI、NT-proBNP联合使用可进一步提高预后预测准确性。 展开更多
关键词 心力衰竭 axl受体酪氨酸激酶 预后 预测 危险因素
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贝母素乙通过调节Gas6/Axl信号通路对糖尿病大鼠心肌纤维化的影响
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作者 王瑞琪 庞国明 李立 《天津中医药》 CAS 2024年第12期1593-1598,共6页
[目的]探讨贝母素乙(PMI)对糖尿病(DM)大鼠心肌纤维化及生长停滞特异性蛋白6(Gas6)/Axl信号通路的影响。[方法]构建DM大鼠模型,将48只造模成功大鼠随机分为DM组、贝母素乙低、高剂量组(PMI-L、PMI-H组)、贝母素乙高剂量+通路抑制剂R428... [目的]探讨贝母素乙(PMI)对糖尿病(DM)大鼠心肌纤维化及生长停滞特异性蛋白6(Gas6)/Axl信号通路的影响。[方法]构建DM大鼠模型,将48只造模成功大鼠随机分为DM组、贝母素乙低、高剂量组(PMI-L、PMI-H组)、贝母素乙高剂量+通路抑制剂R428组(PMI-H+R428组),各12只,另取12只正常大鼠作对照组(Control组);超声检查各组大鼠心功能指标;全自动血液分析仪检测各组大鼠空腹血糖(FBG)及血清肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)水平;苏木精-伊红(HE)染色及Masson染色分别观察各组大鼠心肌组织病理变化及纤维化程度;TUNEL染色检测心肌组织细胞凋亡情况;免疫组化检测胶原蛋白Ⅰ(COLⅠ)、胶原蛋白Ⅲ(COLⅢ)表达;蛋白免疫印迹法检测Gas6、p-Axl/Axl表达。[结果]DM组较Control组心肌纤维断裂,心肌细胞排列紊乱,细胞肥大,细胞核固缩深染,炎性细胞浸润明显,细胞减少,蓝色胶原纤维沉积增多,且排列紊乱,分布弥散,左室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)和FBG、CK-MB、LDH水平及COLⅠ、COLⅢ表达升高,LVFS及Gas6、p-Axl/Axl表达降低(P<0.05);PMI-L、PMI-H组较DM组心肌纤维排列相对整齐,细胞形态相对正常,炎性细胞浸润减少,细胞增多,纤维化现象减少,蓝色胶原纤维沉积减少,且细胞排列相对整齐,LVEDD、LVESD和FBG、CK-MB、LDH水平及COLⅠ、COLⅢ表达降低,LVFS及Gas6、p-Axl/Axl表达升高(P<0.05);PMI-H+R248组较PMI-H组心肌组织病理损伤加重,炎性细胞浸润及心肌组织纤维化明显,蓝色胶原纤维沉积明显增多,LVEDD、LVESD和FBG、CK-MB、LDH水平及COLⅠ、COLⅢ表达升高,LVFS及Gas6、p-Axl/Axl表达降低(P<0.05)。[结论]贝母素乙可抑制DM大鼠心肌纤维化,其作用机制与激活GAS6/Axl信号通路有关。 展开更多
关键词 贝母素乙 生长停滞特异性蛋白6/axl信号通路 糖尿病 心肌纤维化
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熊果苷调节Gas6/Axl信号通路对缺氧缺血性脑损伤新生大鼠的神经保护作用 被引量:2
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作者 王晓静 敖雪 +1 位作者 张宏燕 马兵 《中国优生与遗传杂志》 2024年第2期261-266,共6页
目的 研究熊果苷通过调节Gas6/Axl信号通路对缺氧缺血性脑损伤(HIBD)新生大鼠神经保护的作用。方法 采用Rice-Vannucci法构建HIBD新生大鼠模型,并将造模成功的48只新生大鼠随机分为Model组、低剂量熊果苷组(50 mg/kg)、高剂量熊果苷组(1... 目的 研究熊果苷通过调节Gas6/Axl信号通路对缺氧缺血性脑损伤(HIBD)新生大鼠神经保护的作用。方法 采用Rice-Vannucci法构建HIBD新生大鼠模型,并将造模成功的48只新生大鼠随机分为Model组、低剂量熊果苷组(50 mg/kg)、高剂量熊果苷组(100 mg/kg)、高剂量熊果苷+Anticancer agent 109组(100 mg/kg+3.00 mg/kg Gas6/Axl抑制剂);再选12只新生大鼠作为NC组。平衡木法对各组大鼠进行行为学评估;苏木精-伊红(H-E)染色检测大鼠脑组织病理损伤;TTC染色法检测脑梗死面积;ELISA法检测大鼠血清中白细胞介素6(IL-6)、超氧化物歧化酶(SOD)、丙二醛(MDA)、脑源性神经营养因子(BDNF)的表达;Western blot检测大鼠海马组织中Gas6、磷酸化Axl(p-Axl)、Axl蛋白表达。结果 与Model组比较,低剂量熊果苷组和高剂量熊果苷组大鼠行为学评分降低,脑组织结构较正常,大鼠脑梗死面积减少,血清中IL-6、MDA表达减少,SOD、BDNF表达增加,Gas6、Axl蛋白表达增多,p-Axl蛋白表达减少(P<0.05)。高剂量熊果苷+Anticancer agent 109组与高剂量熊果苷组比较,结果相反,HIBD大鼠神经损伤加重(P<0.05)。结论 熊果苷可能通过激活Gas6/Axl信号通路对缺氧缺血性脑损伤新生大鼠发挥神经保护作用。 展开更多
关键词 熊果苷 Gas6/axl信号通路 缺氧缺血性脑损伤 神经保护
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Establishment of the thermo-mechanical coupling model of axle box bearings with track irregularity excitation and analysis of its temperature characteristics
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作者 Min WANG Shaopu YANG +2 位作者 Yongqiang LIU Yanhong CHEN Kai ZHANG 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2024年第11期1965-1986,共22页
As an important component of the running gear of high-speed trains,axle box bearings can cause lubricating grease failure and damage to bearing components under continuous high-temperature operation,which will affect ... As an important component of the running gear of high-speed trains,axle box bearings can cause lubricating grease failure and damage to bearing components under continuous high-temperature operation,which will affect the normal operation of highspeed trains.Therefore,bearing temperature is one of the key parameters to be monitored in the online monitoring system for trains.Based on the thermal network method,this paper establishes a thermal network model for the axle box bearing,considering the radial thermal deformation of the double-row tapered roller bearing components caused by the oil film characteristics and the temperature variations of the lubricating grease.A thermo-mechanical coupling model for the grease-lubricated double-row tapered roller axle box bearing of high-speed trains with track irregularity excitation is established.The correctness of the model is verified using the test bench data,and the temperature of the bearing at different rotational speeds,loads,fault sizes,and ambient temperatures are investigated. 展开更多
关键词 axle box bearing thermal network method TEMPERATURE rotational speed
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Instrumented oscillographic study on impact toughness of an axle steel DZ2 with different tempering temperatures
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作者 Shuo Liu Peng Zhang +6 位作者 Bin Wang Kaizhong Wang Zikuan Xu Fangzhong Hu Xin Bai Qiqiang Duan Zhefeng Zhang 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第7期1590-1598,共9页
Compared with the conventional Charpy impact test method,the oscillographic impact test can help in the behavioral analysis of materials during the fracture process.In this study,the trade-off relationship between the... Compared with the conventional Charpy impact test method,the oscillographic impact test can help in the behavioral analysis of materials during the fracture process.In this study,the trade-off relationship between the strength and toughness of a DZ2 axle steel at various tempering temperatures and the cause of the improvement in impact toughness was evaluated.The tempering process dramatically influenced carbide precipitation behavior,which resulted in different aspect ratios of carbides.Impact toughness improved along with the rise in tempering temperature mainly due to the increase in energy required in impact crack propagation.The characteristics of the impact crack propagation process were studied through a comprehensive analysis of stress distribution,oscilloscopic impact statistics,fracture morphology,and carbide morphology.The poor impact toughness of low-tempering-temperature specimens was attributed to the increased number of stress concentration points caused by carbide morphology in the small plastic zone during the propagation process,which resulted in a mixed distribution of brittle and ductile fractures on the fracture surface. 展开更多
关键词 axle steel DZ2 tempering process impact toughness oscillographic impact test impact crack propagation carbides
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血清Decorin、sAxl和CYTL1水平与膝骨关节炎患者K-L分级的关系研究
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作者 胡国新 李太极 刘春晖 《国际检验医学杂志》 CAS 2024年第17期2132-2135,2140,共5页
目的探讨血清核心蛋白聚糖(Decorin)、可溶性Axl(sAxl)和细胞因子样蛋白1(CYTL1)水平与膝骨关节炎患者Kellgren-Lawrence(K-L)分级的关系。方法选取2021年8月至2023年5月该院收治的膝骨关节炎患者328例作为研究组,另选取同期体检健康者... 目的探讨血清核心蛋白聚糖(Decorin)、可溶性Axl(sAxl)和细胞因子样蛋白1(CYTL1)水平与膝骨关节炎患者Kellgren-Lawrence(K-L)分级的关系。方法选取2021年8月至2023年5月该院收治的膝骨关节炎患者328例作为研究组,另选取同期体检健康者328例作为对照组。采用酶联免疫吸附试验检测血清Decorin、sAxl和CYTL1水平,西安大略和麦克马斯特大学骨性关节炎调查量表(WOMAC)评估患者临床症状。采用Spearman法分析血清Decorin、sAxl和CYTL1与K-L分级和WOMAC评分的相关性,Logistic回归分析膝骨关节炎的影响因素,绘制受试者工作特征(ROC)曲线分析血清Decorin、sAxl和CYTL1对膝骨关节炎Ⅰ~Ⅱ级、Ⅲ~Ⅳ级的诊断价值。结果研究组血清Decorin、sAxl水平显著高于对照组,CYTL1显著低于对照组,差异均有统计学意义(P<0.05)。K-L分级Ⅰ级、Ⅱ级、Ⅲ级、Ⅳ级血清Decorin、sAxl水平和WOMAC评分依次升高(P<0.05),CYTL1水平依次降低(P<0.05)。Spearman相关性分析显示,血清Decorin、sAxl与K-L分级和WOMAC评分均呈正相关(P<0.05),CYTL1与K-L分级和WOMAC评分呈负相关(P<0.05)。Decorin、sAxl高水平、CYTL1低水平为膝骨关节炎的危险因素(P<0.05)。血清Decorin、sAxl和CYTL1水平对膝骨关节炎Ⅰ~Ⅱ级、Ⅲ~Ⅳ级有一定诊断价值。结论血清Decorin、sAxl和CYTL1水平与膝骨关节炎K-L分级密切相关,均可用于膝骨关节炎Ⅰ~Ⅱ级、Ⅲ~Ⅳ级的辅助诊断。 展开更多
关键词 膝骨关节炎 核心蛋白聚糖 可溶性axl 细胞因子样蛋白1 Kellgren-Lawrence分级
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Optimal allocation method of electric/air braking force of high-speed train considering axle load transfer
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作者 Feng Guo Jing He 《High-Speed Railway》 2024年第2期77-84,共8页
Reasonable distribution of braking force is a factor for a smooth,safe,and comfortable braking of trains.A dynamic optimal allocation strategy of electric-air braking force is proposed in this paper to solve the probl... Reasonable distribution of braking force is a factor for a smooth,safe,and comfortable braking of trains.A dynamic optimal allocation strategy of electric-air braking force is proposed in this paper to solve the problem of the lack of consideration of adhesion difference of train wheelsets in the existing high-speed train electric-air braking force optimal allocation strategies.In this method,the braking strategy gives priority to the use of electric braking force.The force model of a single train in the braking process is analyzed to calculate the change of adhesion between the wheel and rail of each wheelset after axle load transfer,and then the adhesion of the train is estimated in real time.Next,with the goal of maximizing the total adhesion utilization ratio of trailer/motor vehicles,a linear programming distribution function is constructed.The proportional coefficient of adhesion utilization ratio of each train and the application upper limit of braking force in the function is updated according to the change time point of wheelset adhesion.Finally,the braking force is dynamically allocated.The simulation results of Matlab/Simulink show that the proposed algorithm not only uses the different adhesion limits of each trailer to reduce the total amount of braking force undertaken by the motor vehicle,but also considers the adhesion difference of each wheelset.The strategy can effectively reduce the risk and time of motor vehicles during the braking process and improve the stability of the train braking. 展开更多
关键词 Braking force allocation WHEELSET Dynamicity axle load transfer total Adhesion utilization ratio
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Effect of Seasonal Variations on the Behavior of Flexible Pavements in Burkina Faso: Towards Alternating and Periodic Loading of Multi-Axle Heavy Goods Vehicles for Road Durability
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作者 Kokoro Kobori Doua Allain Gnabahou Bouto Kossi Imbga 《Journal of Materials Science and Chemical Engineering》 2024年第6期24-42,共19页
Bituminous materials are heat-sensitive, and their mechanical properties vary with temperature. This variation in properties is not without consequences on the performance of flexible road structures under the repeate... Bituminous materials are heat-sensitive, and their mechanical properties vary with temperature. This variation in properties is not without consequences on the performance of flexible road structures under the repeated passage of multi-axles. This study determines the influence of seasonal variations on the rate of permanent deformation, the rut depth of flexible pavements and the effect of alternating loading of heavy goods vehicles following the temperature variations on the durability of roads. Thus, an ambient and pavement surface temperature measurement was carried out in 2022. The temperature profile at different layers of the modelled pavement, the evaluation of deformation rates and rutting depth were determined using several models. The results show that the permanent deformation and rutting rates are higher at the level of the bituminous concrete layer than at the level of the asphalt gravel layer because the stresses decrease from the surface to the depth of the pavement. On the other hand, the variations in these rates, permanent deformations and ruts between the hot and so-called cold periods are more pronounced in the bitumen gravel than in bituminous concrete, showing that gravel bitumen is more sensitive to temperature variations than bituminous concrete despite its higher rigidity. Of these results, we suggested a periodic and alternating loading of the different types of heavy goods vehicles. These loads consist of fully applying the WAEMU standards with a tolerance of 15% during periods of high and low temperatures. This regulation has increased 2 to 3 times in the durability of roadways depending on the type of heavy goods vehicle. 展开更多
关键词 PAVEMENT RUTTING Permanent Deformation Multi-axle Seasonal
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Axl调控的miRNA表达在NSCLC吉非替尼获得性耐药中的意义 被引量:7
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作者 曹新广 吕慧芳 +6 位作者 陈贝贝 常吉梅 唐菲 朱彩华 樊鑫鑫 秦建军 陈小兵 《郑州大学学报(医学版)》 CAS 北大核心 2015年第5期651-655,共5页
目的:探讨酪氨酸激酶Axl调控的miRNA在非小细胞肺癌(NSCLC)吉非替尼获得性耐药中的意义。方法:比较表皮生长因子受体(EGFR)突变型NSCLC细胞株HCC827及其吉非替尼耐药株HCC827-Gef中Axl mRNA[实时荧光定量PCR(qRT-PCR)]和蛋白(Western bl... 目的:探讨酪氨酸激酶Axl调控的miRNA在非小细胞肺癌(NSCLC)吉非替尼获得性耐药中的意义。方法:比较表皮生长因子受体(EGFR)突变型NSCLC细胞株HCC827及其吉非替尼耐药株HCC827-Gef中Axl mRNA[实时荧光定量PCR(qRT-PCR)]和蛋白(Western blot)的表达,再利用微阵列分析筛选出20个差异表达miRNA,其中miR-374a、miR-548b敏感度最高;采用qRT-PCR检测Axl沉默对HCC827-Gef细胞miR-374a、miR-548b表达的影响;对不同Axl mRNA表达水平的NSCLC患者(共40例)miR-374a、miR-548b表达进行比较并进行Kaplan-Meier法生存分析。结果:HCC827-Gef细胞中Axl mRNA及蛋白的表达均高于HCC827细胞(t=9.393,24.038;P<0.001)。与转染空质粒的HCC827-Gef细胞比较,HCC827-Gef-esiA XL细胞中Axl mRNA、miR-374a表达下降,miR-548b表达则上调(t=22.155,56.000,16.000;P<0.001)。与Axl低表达的NSCLC患者比较,Axl mRNA高表达者癌组织中miR-374a表达升高,miR-548b表达降低(t=5.231,2.473;P<0.05),无病生存期缩短(χ2=6.550,P=0.011)。结论:Axl及其调控的miR-374a和miR-548b在NSCLC吉非替尼获得性耐药中起重要作用,可作为NSCLC预后标记或潜在的治疗靶点。 展开更多
关键词 非小细胞肺癌 吉非替尼 axl MIRNA 耐药
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