The aim of the study was to clarify the role of Aurka in the process of formation and development of gastric cancer. The expression of Aurka in gastric cancer and corresponding non-cancerous gastric tissues of 52 gast...The aim of the study was to clarify the role of Aurka in the process of formation and development of gastric cancer. The expression of Aurka in gastric cancer and corresponding non-cancerous gastric tissues of 52 gastric cancer patients was assessed with the real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry. We also analyzed the relationship between the expression level and clinicopathological characteristics. Aurka gene and protein expression in gastric cancer tissues in both cases were significantly higher than in corresponding non-cancerous gastric tissues (both P < 0.01), but no significant relationship was found with clinicopathological parameters including tumor location, depth of invasion, differentiation, lymph node metastasis, stage, gender, age and carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9) level in peripheral blood preoperation of patients (P > 0.05, respectively). Furthermore, Aurka gene expression was markedly higher in gastric cancer tissues of Helicobacter pylori (HP)-positive patients than negative ones (P < 0.05). The result of the study showed that Aurka might play a significant role in the process of formation and development of gastric cancer as an oncogene. Its effect might be strengthened by HP infection.展开更多
目的检测极光激酶A(AURKA)基因在口腔鳞癌中的表达及意义。方法收集20例口腔正常组织及42例口腔鳞状细胞癌(OSCC)组织,采用Real-Time PCR检测AURKA基因在不同口腔组织中的表达。结果 AURKA mRNA在OSCC中高表达,且不同分化程度,不同临床...目的检测极光激酶A(AURKA)基因在口腔鳞癌中的表达及意义。方法收集20例口腔正常组织及42例口腔鳞状细胞癌(OSCC)组织,采用Real-Time PCR检测AURKA基因在不同口腔组织中的表达。结果 AURKA mRNA在OSCC中高表达,且不同分化程度,不同临床分期,组间差异有统计学意义。不同年龄,不同性别,组间差异无统计学意义。结论 AURKA在OSCC中高表达,AURKA作为激酶可能直接或间接地激活多种致癌蛋白或使多种抑癌蛋白失活,从而推动肿瘤的发生发展。展开更多
文摘目的 筛选宫颈癌组织中差异表达的基因,分析其与宫颈癌预后间的关系,得出其作为宫颈癌预后评估新标志物的可能性。方法 从GEO数据库获取宫颈癌和正常宫颈组织的转录组测序结果的数据。通过在线GEO2R工具分析得到宫颈癌和正常宫颈组织中的差异表达基因。用“仙桃学术”进行GO和KEGG分析。基于String网站和Cytoscape软件构建蛋白-蛋白相互作用(PPI)网络并筛选出枢纽基因。通过Kaplan-Meier和Cox单因素回归分析枢纽基因对患者总生存期(OS)和无复发生存期(RFS)的影响及与其临床特征的关联。通过在线数据库GEPIA database,the Human Protein Atlas和临床样本进行枢纽基因表达的验证。采用受试者工作特征(ROC)曲线来评估枢纽基因表达水平对区分宫颈癌组织和正常宫颈组织的可行性。结果 在公共数据集中得到79个差异表达基因,有30个基因表达上调,49个基因表达下调。富集分析显示,AURKA基因主要参与“细胞周期”、“孕酮介导的卵细胞成熟”和“铂耐药性”等通路。蛋白质相互作用网络及枢纽基因生存分析得出AURKA基因的高表达与宫颈癌患者总生存期及无复发生存期较短显著相关(P<0.05)。通过数据库及临床样本进一步验证得出AURKA基因的mRNA和蛋白在宫颈癌中表达显著上调且无种族人群差异。ROC结果显示曲线下面积(AUC)为0.999,表明AURKA基因表达水平对宫颈癌具有较高的预测价值。结论 宫颈癌组织中AURKA mRNA和蛋白表达上调,则宫颈癌患者的总生存期和无复发生存期均缩短,AURKA基因具有作为宫颈癌预后评估标志物及靶点的潜在可能。
文摘The aim of the study was to clarify the role of Aurka in the process of formation and development of gastric cancer. The expression of Aurka in gastric cancer and corresponding non-cancerous gastric tissues of 52 gastric cancer patients was assessed with the real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry. We also analyzed the relationship between the expression level and clinicopathological characteristics. Aurka gene and protein expression in gastric cancer tissues in both cases were significantly higher than in corresponding non-cancerous gastric tissues (both P < 0.01), but no significant relationship was found with clinicopathological parameters including tumor location, depth of invasion, differentiation, lymph node metastasis, stage, gender, age and carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9) level in peripheral blood preoperation of patients (P > 0.05, respectively). Furthermore, Aurka gene expression was markedly higher in gastric cancer tissues of Helicobacter pylori (HP)-positive patients than negative ones (P < 0.05). The result of the study showed that Aurka might play a significant role in the process of formation and development of gastric cancer as an oncogene. Its effect might be strengthened by HP infection.
文摘目的检测极光激酶A(AURKA)基因在口腔鳞癌中的表达及意义。方法收集20例口腔正常组织及42例口腔鳞状细胞癌(OSCC)组织,采用Real-Time PCR检测AURKA基因在不同口腔组织中的表达。结果 AURKA mRNA在OSCC中高表达,且不同分化程度,不同临床分期,组间差异有统计学意义。不同年龄,不同性别,组间差异无统计学意义。结论 AURKA在OSCC中高表达,AURKA作为激酶可能直接或间接地激活多种致癌蛋白或使多种抑癌蛋白失活,从而推动肿瘤的发生发展。
