[ Objective] The aim of this paper was to investigate the genetic diversity of mitochondrial DNA D-loop sequences of Cheilinus undulatus populations. [Method] Twenty-five individuals of C. undu/atus were collected fro...[ Objective] The aim of this paper was to investigate the genetic diversity of mitochondrial DNA D-loop sequences of Cheilinus undulatus populations. [Method] Twenty-five individuals of C. undu/atus were collected from the offshore area of Hainan Province. PCR and cloning techniques were used to clone and purify the mtDNA D-loop sequences of C. undu/atus populations （n =25）, about 1 400 bp long PCR product was obtained. ClustalX software was adopted for sequence alignment, and results were imported into MEGAS. 0. [ Result] According to experimental results, 66 mutation sites were detected from 25 individuals, including 0 deletion, 4 inserts, 59 transition sites, 2 transversion sites and 1 transition-transversion site. Pairwise genetic distances of these 25 individuals were calcu- lated by using MEGA5.0 software. Based on that, NJ phylogenetic tree of these 25 individuals was constructed. Analysis of C. undulatus populations using DNASP software showed that the polymorphic site number （S） was 62, nucleotide diversity （Pi） was 0.006 06, and average number of nucleotide differences （K） was 6. 907. [ Conclusion] Overall, there was no significant variation among mtDNA D-loops of C. undulatus populations. Key words展开更多
＂The Belt and Road＂ initiative is rooted in history and closely related to cultural heritage. The precious heritage and sites are ＂living fossils＂ that serve as witness of the historical period, symbols of cultura...＂The Belt and Road＂ initiative is rooted in history and closely related to cultural heritage. The precious heritage and sites are ＂living fossils＂ that serve as witness of the historical period, symbols of cultural exchanges and embodiment of the constantly relevant Silk Road spirit. They belong to the whole mankind and play an increasingly important role in promoting people＇s mutual understanding, advancing the Silk Road spirit and boosting the development of the Belt and Road initiative.展开更多
基金Supported by National Natural Science Foundation of China(40966003)Natural Science Foundation of Hainan Province(809009)
文摘[ Objective] The aim of this paper was to investigate the genetic diversity of mitochondrial DNA D-loop sequences of Cheilinus undulatus populations. [Method] Twenty-five individuals of C. undu/atus were collected from the offshore area of Hainan Province. PCR and cloning techniques were used to clone and purify the mtDNA D-loop sequences of C. undu/atus populations （n =25）, about 1 400 bp long PCR product was obtained. ClustalX software was adopted for sequence alignment, and results were imported into MEGAS. 0. [ Result] According to experimental results, 66 mutation sites were detected from 25 individuals, including 0 deletion, 4 inserts, 59 transition sites, 2 transversion sites and 1 transition-transversion site. Pairwise genetic distances of these 25 individuals were calcu- lated by using MEGA5.0 software. Based on that, NJ phylogenetic tree of these 25 individuals was constructed. Analysis of C. undulatus populations using DNASP software showed that the polymorphic site number （S） was 62, nucleotide diversity （Pi） was 0.006 06, and average number of nucleotide differences （K） was 6. 907. [ Conclusion] Overall, there was no significant variation among mtDNA D-loops of C. undulatus populations. Key words
文摘＂The Belt and Road＂ initiative is rooted in history and closely related to cultural heritage. The precious heritage and sites are ＂living fossils＂ that serve as witness of the historical period, symbols of cultural exchanges and embodiment of the constantly relevant Silk Road spirit. They belong to the whole mankind and play an increasingly important role in promoting people＇s mutual understanding, advancing the Silk Road spirit and boosting the development of the Belt and Road initiative.
