In the present study we investigated the impact of the combination of astragalin and rutin(CAR)on restoring gut-microbial dysbiosis and obesity and obesity related disorders.Randomized male C57BL/6J mice were experime...In the present study we investigated the impact of the combination of astragalin and rutin(CAR)on restoring gut-microbial dysbiosis and obesity and obesity related disorders.Randomized male C57BL/6J mice were experimentally divided into 5 groups and fed either a normal diet or a high-fat diet(HFD)for 16 weeks.Compared to vehicle treated group(HFD group),CAR could substantially improve selected gut microbiota abundance(Akkermansia,Lactobacillus,Bifidobacteria,Roseburia,Prevotella),reversed the Firmicutes/Bacteroidetes proportions,and inhibited the growth of Escherichia coli,Salmonella,and Klebsiella in obese mice.In addition,CAR-treated mice showed significantly increased total short-chain fatty acid production,reduced body weight gain,organs’weights,serum lipid profile(except HDL)and insulin resistance.The mRNA expressions of CCAAT/enhancer binding protein-α(C/EBP-α),sterol regulatory element-binding protein-1c(SREBP-1c),peroxisome proliferator-activated receptor-γ(PPAR-γ),acetyl-CoA carboxylase(ACC),adipocyte protein 2(aP2),and fatty acid synthase(FAS)were downregulated(P<0.05)and the protein expression of PPAR-γwas downregulated while adenosine 5’monophosphate-activated protein kinase(AMPK)was phosphorylated in CAR-treated HFD-fed mice compared to the HFD control group.Interestingly,CAR-treated HFD-fed mice showed significantly improved tissue architecture in the liver and fatty tissues.In conclusion,the findings suggest that CAR/Moringa oleifera may be beneficial in the treatment of insulin resistance and obesity disorders.展开更多
[ Objective] The paper was to explore the effect of astragalin on paraoxon-indueed vascular endothelium dysfunction and analyze the potential mecha- nism. [Method]The isolated rat thoracic aorta rings were exposed to ...[ Objective] The paper was to explore the effect of astragalin on paraoxon-indueed vascular endothelium dysfunction and analyze the potential mecha- nism. [Method]The isolated rat thoracic aorta rings were exposed to medium contained paraoxon （3.63 μmol/L）, and astragalin （10 μmol/L） was used to inhib- it the damage effect. Rat thoracic aorta rings were suspended in organ chambers to assess vas orelaxation activity in vitro by acetyleholine （ACh）-induced endotheli- um dependent relaxation reaction （EDRR） and sodium nitroprusside （SNP）-induced endothdium-independent relaxation reaction. [Result]The exposure to parao- xon （3.63 μmol/L） resulted in an inhibition of the EDRR, markedly reduced the level of nitric oxide （NO）, the activity of paraoxonasel （PON1） and superoxide dismutase （SOD）, and significantly increased the level of malondialdehyde （MDA） in isolated rat thoracic aorta. However, the presence of astragalin （10 μmol/L） markedly attenuated the vascular endothelium dysfunction induced by paraoxon via increasing level of NO, activity of PON1 and SOD, as well as reducing level of MDA. In addition, treatment of astragalin （ 10 μmol/L） showed a similar effect to hydrogen peroxide （ 1.0 μmol/L）, a kind of antioxidant, on paraoxon- induced vascular endothelium dysfunction. [ Conclusion] Astragalin could protect the vascular endothelium against the paraoxon-induced dysfunction in isolated rat thoracic aorta, and＇the beneficial effects of astragalin might be concerned with the antioxidation of astragalin due to inhibiting the decreased activity of PONI.展开更多
AIM:To investigate the aldose reductase(AR)inhibition capacity of astragalin(AST)against streptozoticin-induced diabetic cataracts(DCs)in rats.METHODS:Ex vivo investigations were conducted by treating the lens of a go...AIM:To investigate the aldose reductase(AR)inhibition capacity of astragalin(AST)against streptozoticin-induced diabetic cataracts(DCs)in rats.METHODS:Ex vivo investigations were conducted by treating the lens of a goat placed for 72h in artificial aqueous humor(AAH)of pH 7.8 at room temperature with cataract-causing substance(55 mmol/L of galactose)and in vivo studies were performed on rats via induction with streptozotocin.AST was administered at different dose levels and scrutinize for DC activity.RESULTS:In diabetic rats,AST improved the body weight,blood insulin,and glucose as well as the levels of galactitol in a dose-dependent way,other biochemical parameters i.e.inflammatory mediators and cytokines,and also suppress AR activity.The level of the antioxidant parameters such as superoxide dismutase(SOD),catalase(CAT),and glutathione(GSH)activity were also altered on a diabetic lens after the administration of the AST.CONCLUSION:AST protects against lens opacification to avoid cataracts and polyols formation,indicating that it could be used as a potential therapeutic agent for diabetes.展开更多
目的 优选盐沙苑子Astragali Complanati Semen最佳炮制工艺,并对其炮制前后抗炎作用进行研究。方法 在单因素实验基础上,采用L9(34)正交试验设计,结合熵权-优劣解距离法(逼近理想解排序法,technique for order preference by similarit...目的 优选盐沙苑子Astragali Complanati Semen最佳炮制工艺,并对其炮制前后抗炎作用进行研究。方法 在单因素实验基础上,采用L9(34)正交试验设计,结合熵权-优劣解距离法(逼近理想解排序法,technique for order preference by similarity to ideal solution,TOPSIS),以7个黄酮类成分(山柰酚-3-O-桑布双糖苷、紫云英苷、沙苑子苷B、沙苑子苷、杨梅素、山柰酚和山柰素)的含量作为评价指标,得到沙苑子最佳盐炙工艺并进行验证;建立以2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzenesulfonic acid,TNBS)诱导的斑马鱼肠炎模型,对沙苑子盐炙前后抗炎作用进行比较。结果 优选得出的沙苑子最佳盐炙工艺为每30克沙苑子,加2%食盐水4 m L,闷润8 h,于100℃炒15 min;药效实验结果表明,沙苑子盐炙后对斑马鱼肠炎模型肠道组织紊乱的改善及降低肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、IL-6和IL-8释放量等方面略优于生品。结论 熵权-TOPSIS法优选得到的盐沙苑子炮制工艺稳定、可行。与模型组相比,盐沙苑子水提物展现出显著的抗炎活性,可为阐明盐沙苑子的炮制增效机制提供参考。展开更多
Objective To investigate the effect of Astragalin on human renal mesangial cells Methods Cultured human mesangial cells were treated with Astragalin and Astragalin serum in different concentrations in the presence ...Objective To investigate the effect of Astragalin on human renal mesangial cells Methods Cultured human mesangial cells were treated with Astragalin and Astragalin serum in different concentrations in the presence or absence of PDGF BB, the proliferation and type Ⅳ collagen secretion of mesangial cells were measured by MTT assay and ELISA, and expression of β1 integrin gene was estimated by reverse transcription polymerase chain reaction (RT PCR) method, sespectively Results After 72 hours Astragalin or Astragalin serum treatment, the proliferation of mesangial cells induced by PDGF BB was inhibited significantly in a dose dependent manner compared with untreated controls ( P <0 05 and P <0 01) After 24 hours of Astragalin or Astragalin serum treatment, the secretion of type Ⅳ collagen protein in presence of PDGF BB was significantly decreased and β1 integrin mRNA level decreased significantly compared with untreated control ( P <0 05, P <0 01) Conclusions Astragalin inhibits cell proliferation and matrix over synthesis which might be mediated, at least, partly by decrease of β1 integrin gene over expression The study suggested that Astragalin might play a role in preventing the progression of chronic renal diseases展开更多
基金Authors are grateful to the Department of Science and Technology,New Delhi,for financial support(DST-SERB,EEQ/2016/000123,2016-17),and thank DST-FIST,Department of Biochemistry,S.V.University.
文摘In the present study we investigated the impact of the combination of astragalin and rutin(CAR)on restoring gut-microbial dysbiosis and obesity and obesity related disorders.Randomized male C57BL/6J mice were experimentally divided into 5 groups and fed either a normal diet or a high-fat diet(HFD)for 16 weeks.Compared to vehicle treated group(HFD group),CAR could substantially improve selected gut microbiota abundance(Akkermansia,Lactobacillus,Bifidobacteria,Roseburia,Prevotella),reversed the Firmicutes/Bacteroidetes proportions,and inhibited the growth of Escherichia coli,Salmonella,and Klebsiella in obese mice.In addition,CAR-treated mice showed significantly increased total short-chain fatty acid production,reduced body weight gain,organs’weights,serum lipid profile(except HDL)and insulin resistance.The mRNA expressions of CCAAT/enhancer binding protein-α(C/EBP-α),sterol regulatory element-binding protein-1c(SREBP-1c),peroxisome proliferator-activated receptor-γ(PPAR-γ),acetyl-CoA carboxylase(ACC),adipocyte protein 2(aP2),and fatty acid synthase(FAS)were downregulated(P<0.05)and the protein expression of PPAR-γwas downregulated while adenosine 5’monophosphate-activated protein kinase(AMPK)was phosphorylated in CAR-treated HFD-fed mice compared to the HFD control group.Interestingly,CAR-treated HFD-fed mice showed significantly improved tissue architecture in the liver and fatty tissues.In conclusion,the findings suggest that CAR/Moringa oleifera may be beneficial in the treatment of insulin resistance and obesity disorders.
基金Supported by Natural Sciences Research Project of Henan Province(No.2010B320011,No.2010B330002)~~
文摘[ Objective] The paper was to explore the effect of astragalin on paraoxon-indueed vascular endothelium dysfunction and analyze the potential mecha- nism. [Method]The isolated rat thoracic aorta rings were exposed to medium contained paraoxon （3.63 μmol/L）, and astragalin （10 μmol/L） was used to inhib- it the damage effect. Rat thoracic aorta rings were suspended in organ chambers to assess vas orelaxation activity in vitro by acetyleholine （ACh）-induced endotheli- um dependent relaxation reaction （EDRR） and sodium nitroprusside （SNP）-induced endothdium-independent relaxation reaction. [Result]The exposure to parao- xon （3.63 μmol/L） resulted in an inhibition of the EDRR, markedly reduced the level of nitric oxide （NO）, the activity of paraoxonasel （PON1） and superoxide dismutase （SOD）, and significantly increased the level of malondialdehyde （MDA） in isolated rat thoracic aorta. However, the presence of astragalin （10 μmol/L） markedly attenuated the vascular endothelium dysfunction induced by paraoxon via increasing level of NO, activity of PON1 and SOD, as well as reducing level of MDA. In addition, treatment of astragalin （ 10 μmol/L） showed a similar effect to hydrogen peroxide （ 1.0 μmol/L）, a kind of antioxidant, on paraoxon- induced vascular endothelium dysfunction. [ Conclusion] Astragalin could protect the vascular endothelium against the paraoxon-induced dysfunction in isolated rat thoracic aorta, and＇the beneficial effects of astragalin might be concerned with the antioxidation of astragalin due to inhibiting the decreased activity of PONI.
文摘AIM:To investigate the aldose reductase(AR)inhibition capacity of astragalin(AST)against streptozoticin-induced diabetic cataracts(DCs)in rats.METHODS:Ex vivo investigations were conducted by treating the lens of a goat placed for 72h in artificial aqueous humor(AAH)of pH 7.8 at room temperature with cataract-causing substance(55 mmol/L of galactose)and in vivo studies were performed on rats via induction with streptozotocin.AST was administered at different dose levels and scrutinize for DC activity.RESULTS:In diabetic rats,AST improved the body weight,blood insulin,and glucose as well as the levels of galactitol in a dose-dependent way,other biochemical parameters i.e.inflammatory mediators and cytokines,and also suppress AR activity.The level of the antioxidant parameters such as superoxide dismutase(SOD),catalase(CAT),and glutathione(GSH)activity were also altered on a diabetic lens after the administration of the AST.CONCLUSION:AST protects against lens opacification to avoid cataracts and polyols formation,indicating that it could be used as a potential therapeutic agent for diabetes.
文摘目的 优选盐沙苑子Astragali Complanati Semen最佳炮制工艺,并对其炮制前后抗炎作用进行研究。方法 在单因素实验基础上,采用L9(34)正交试验设计,结合熵权-优劣解距离法(逼近理想解排序法,technique for order preference by similarity to ideal solution,TOPSIS),以7个黄酮类成分(山柰酚-3-O-桑布双糖苷、紫云英苷、沙苑子苷B、沙苑子苷、杨梅素、山柰酚和山柰素)的含量作为评价指标,得到沙苑子最佳盐炙工艺并进行验证;建立以2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzenesulfonic acid,TNBS)诱导的斑马鱼肠炎模型,对沙苑子盐炙前后抗炎作用进行比较。结果 优选得出的沙苑子最佳盐炙工艺为每30克沙苑子,加2%食盐水4 m L,闷润8 h,于100℃炒15 min;药效实验结果表明,沙苑子盐炙后对斑马鱼肠炎模型肠道组织紊乱的改善及降低肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、IL-6和IL-8释放量等方面略优于生品。结论 熵权-TOPSIS法优选得到的盐沙苑子炮制工艺稳定、可行。与模型组相比,盐沙苑子水提物展现出显著的抗炎活性,可为阐明盐沙苑子的炮制增效机制提供参考。
文摘Objective To investigate the effect of Astragalin on human renal mesangial cells Methods Cultured human mesangial cells were treated with Astragalin and Astragalin serum in different concentrations in the presence or absence of PDGF BB, the proliferation and type Ⅳ collagen secretion of mesangial cells were measured by MTT assay and ELISA, and expression of β1 integrin gene was estimated by reverse transcription polymerase chain reaction (RT PCR) method, sespectively Results After 72 hours Astragalin or Astragalin serum treatment, the proliferation of mesangial cells induced by PDGF BB was inhibited significantly in a dose dependent manner compared with untreated controls ( P <0 05 and P <0 01) After 24 hours of Astragalin or Astragalin serum treatment, the secretion of type Ⅳ collagen protein in presence of PDGF BB was significantly decreased and β1 integrin mRNA level decreased significantly compared with untreated control ( P <0 05, P <0 01) Conclusions Astragalin inhibits cell proliferation and matrix over synthesis which might be mediated, at least, partly by decrease of β1 integrin gene over expression The study suggested that Astragalin might play a role in preventing the progression of chronic renal diseases
