Downregulation of the inwardly rectifying potassium channel Kir4.1 is a key step for inducing retinal Müller cell activation and interaction with other glial cells,which is involved in retinal ganglion cell apopt...Downregulation of the inwardly rectifying potassium channel Kir4.1 is a key step for inducing retinal Müller cell activation and interaction with other glial cells,which is involved in retinal ganglion cell apoptosis in glaucoma.Modulation of Kir4.1 expression in Müller cells may therefore be a potential strategy for attenuating retinal ganglion cell damage in glaucoma.In this study,we identified seven predicted phosphorylation sites in Kir4.1 and constructed lentiviral expression systems expressing Kir4.1 mutated at each site to prevent phosphorylation.Following this,we treated Müller glial cells in vitro and in vivo with the m Glu R I agonist DHPG to induce Kir4.1 or Kir4.1 Tyr^(9)Asp overexpression.We found that both Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression inhibited activation of Müller glial cells.Subsequently,we established a rat model of chronic ocular hypertension by injecting microbeads into the anterior chamber and overexpressed Kir4.1 or Kir4.1 Tyr^(9)Asp in the eye,and observed similar results in Müller cells in vivo as those seen in vitro.Both Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression inhibited Müller cell activation,regulated the balance of Bax/Bcl-2,and reduced the m RNA and protein levels of pro-inflammatory factors,including interleukin-1βand tumor necrosis factor-α.Furthermore,we investigated the regulatory effects of Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression on the release of pro-inflammatory factors in a co-culture system of Müller glial cells and microglia.In this co-culture system,we observed elevated adenosine triphosphate concentrations in activated Müller cells,increased levels of translocator protein(a marker of microglial activation),and elevated interleukin-1βm RNA and protein levels in microglia induced by activated Müller cells.These changes could be reversed by Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression in Müller cells.Kir4.1 overexpression,but not Kir4.1 Tyr^(9)Asp overexpression,reduced the number of proliferative and migratory microglia induced by activated Müller cells.Collectively,these results suggest that the tyrosine residue at position nine in Kir4.1 may serve as a functional modulation site in the retina in an experimental model of glaucoma.Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression attenuated Müller cell activation,reduced ATP/P2X receptor–mediated interactions between glial cells,inhibited microglial activation,and decreased the synthesis and release of pro-inflammatory factors,consequently ameliorating retinal ganglion cell apoptosis in glaucoma.展开更多
ASP.NET-based agricultural machinery monitoring WEBGIS is flexible and dynamic,but this flexibility and dynamic characteristics reduce the performance of WEBGIS.Therefore,it is necessary to use built-in optimization f...ASP.NET-based agricultural machinery monitoring WEBGIS is flexible and dynamic,but this flexibility and dynamic characteristics reduce the performance of WEBGIS.Therefore,it is necessary to use built-in optimization features of.NET Framework,some performance optimization techniques in program design and ASP.NET cache technology to reduce the loading of server,and make the designed system work more efficiently.展开更多
Based on ASP.NET,a orange fruit tree fertilizer expert system software was developed.The system could simulate and decide an annual fertilization plan for young and mature trees in terms of geographical position and c...Based on ASP.NET,a orange fruit tree fertilizer expert system software was developed.The system could simulate and decide an annual fertilization plan for young and mature trees in terms of geographical position and climate.This paper introduced the design conditions,framework,production,and deployment of the system.It exhibited characters of orange specialty and was a typical online agriculture expert system.The use of the system for orange fruit management could decrease production cost,guarantee orange quality and improve economical benefit at the same time.Farmer using the system saved N input by 41-238 g/plant,P2O5 input 3-24 g/plant,and K2O input 1-36 g/plant,and got higher yield by 6-17 kg/plant.展开更多
随着Internet的日益发展、普及,人们对WEB的要求越来越高,需要灵活的动态网页技术。动态网页技术ASP具有保密性好、与所有脚本语言兼容、易编写、无需编译等特点,且ASP在与数据库的连接上采用ADO(ActiveX Data Objects)技术访问后台数据...随着Internet的日益发展、普及,人们对WEB的要求越来越高,需要灵活的动态网页技术。动态网页技术ASP具有保密性好、与所有脚本语言兼容、易编写、无需编译等特点,且ASP在与数据库的连接上采用ADO(ActiveX Data Objects)技术访问后台数据库,这些深受网页制作者的欢迎。在Online Testing System中动态网页制作中采用ASP,具有一定实用价值。展开更多
基金supported by the National Natural Science Foundation of China,Nos.32271043(to ZW)and 82171047(to YM)the both Science and Technology Major Project of Shanghai,No.2018SHZDZX01 and ZJLabShanghai Center for Brain Science and Brain-Inspired Technology(to ZW)。
文摘Downregulation of the inwardly rectifying potassium channel Kir4.1 is a key step for inducing retinal Müller cell activation and interaction with other glial cells,which is involved in retinal ganglion cell apoptosis in glaucoma.Modulation of Kir4.1 expression in Müller cells may therefore be a potential strategy for attenuating retinal ganglion cell damage in glaucoma.In this study,we identified seven predicted phosphorylation sites in Kir4.1 and constructed lentiviral expression systems expressing Kir4.1 mutated at each site to prevent phosphorylation.Following this,we treated Müller glial cells in vitro and in vivo with the m Glu R I agonist DHPG to induce Kir4.1 or Kir4.1 Tyr^(9)Asp overexpression.We found that both Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression inhibited activation of Müller glial cells.Subsequently,we established a rat model of chronic ocular hypertension by injecting microbeads into the anterior chamber and overexpressed Kir4.1 or Kir4.1 Tyr^(9)Asp in the eye,and observed similar results in Müller cells in vivo as those seen in vitro.Both Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression inhibited Müller cell activation,regulated the balance of Bax/Bcl-2,and reduced the m RNA and protein levels of pro-inflammatory factors,including interleukin-1βand tumor necrosis factor-α.Furthermore,we investigated the regulatory effects of Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression on the release of pro-inflammatory factors in a co-culture system of Müller glial cells and microglia.In this co-culture system,we observed elevated adenosine triphosphate concentrations in activated Müller cells,increased levels of translocator protein(a marker of microglial activation),and elevated interleukin-1βm RNA and protein levels in microglia induced by activated Müller cells.These changes could be reversed by Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression in Müller cells.Kir4.1 overexpression,but not Kir4.1 Tyr^(9)Asp overexpression,reduced the number of proliferative and migratory microglia induced by activated Müller cells.Collectively,these results suggest that the tyrosine residue at position nine in Kir4.1 may serve as a functional modulation site in the retina in an experimental model of glaucoma.Kir4.1 and Kir4.1 Tyr^(9)Asp overexpression attenuated Müller cell activation,reduced ATP/P2X receptor–mediated interactions between glial cells,inhibited microglial activation,and decreased the synthesis and release of pro-inflammatory factors,consequently ameliorating retinal ganglion cell apoptosis in glaucoma.
基金Supported by National High Technology Research and Development Program of China(2006AA10A310)Key Task Project in Scientific and Technological Research in Heilongjing Province(GB06B601)Innovation Fund in Daqing Hi-tech Zone(DQGX07YF012)~~
文摘ASP.NET-based agricultural machinery monitoring WEBGIS is flexible and dynamic,but this flexibility and dynamic characteristics reduce the performance of WEBGIS.Therefore,it is necessary to use built-in optimization features of.NET Framework,some performance optimization techniques in program design and ASP.NET cache technology to reduce the loading of server,and make the designed system work more efficiently.
基金fund by the Major Science and Technology Program (2009ZX07102-004),Chinathe IPNI (International Plant Nutrition Institute) Program,Canada (2009ZX07102-004)
文摘Based on ASP.NET,a orange fruit tree fertilizer expert system software was developed.The system could simulate and decide an annual fertilization plan for young and mature trees in terms of geographical position and climate.This paper introduced the design conditions,framework,production,and deployment of the system.It exhibited characters of orange specialty and was a typical online agriculture expert system.The use of the system for orange fruit management could decrease production cost,guarantee orange quality and improve economical benefit at the same time.Farmer using the system saved N input by 41-238 g/plant,P2O5 input 3-24 g/plant,and K2O input 1-36 g/plant,and got higher yield by 6-17 kg/plant.
文摘随着Internet的日益发展、普及,人们对WEB的要求越来越高,需要灵活的动态网页技术。动态网页技术ASP具有保密性好、与所有脚本语言兼容、易编写、无需编译等特点,且ASP在与数据库的连接上采用ADO(ActiveX Data Objects)技术访问后台数据库,这些深受网页制作者的欢迎。在Online Testing System中动态网页制作中采用ASP,具有一定实用价值。
