本研究以新疆库尔勒香梨园随机选取的三棵香梨树花朵为试验材料,采用Illumina Hi Seq/Mi Seq测序技术分离得到1个库尔勒香梨上的苹果茎沟病毒分离物ASGV-kfp的全长序列。序列分析结果显示:该分离物基因全长6 460 bp(ASGV-kfp,KR106996)...本研究以新疆库尔勒香梨园随机选取的三棵香梨树花朵为试验材料,采用Illumina Hi Seq/Mi Seq测序技术分离得到1个库尔勒香梨上的苹果茎沟病毒分离物ASGV-kfp的全长序列。序列分析结果显示:该分离物基因全长6 460 bp(ASGV-kfp,KR106996),包含3个完整的ORF,分别编码polyprotein、MP基因(Movement protein gene)、CP基因(Trichovirus coat protein gene)。ASGV-kfp的CP基因和MP基因与国内外已报道的该病毒的CP基因和MP基因序列同源性分别达到了93%~99%和97%~100%。进化树分析显示,ASGV的CP基因分子变异与寄主以及地理区域没有相关性;ASGV的MP基因分子变异与寄主有一定的相关性。研究结果可为进一步研究ASGV株系分化奠定良好基础。展开更多
Based on growth level and peroxidase activity,a comparative study and paried-samples t test were made between virus-free Zaosu pear seedlings and the seedlings inoculated with apple stem grooving virus(ASGV). The resu...Based on growth level and peroxidase activity,a comparative study and paried-samples t test were made between virus-free Zaosu pear seedlings and the seedlings inoculated with apple stem grooving virus(ASGV). The results showed : (1) virus-free Zaosu pear seedlings grew better than inoculated ones. Of the seedlings the height,diameter,branches and autumn braches differed statistically significantly; (2)the peroxidase activity of inoculated seedlings was higher than that of virus-free ones,and the peak of peroxidase activity occurred on about d 7 and d 24 after inoculation. Fig 1,Tab 1,Ref展开更多
文摘本研究以新疆库尔勒香梨园随机选取的三棵香梨树花朵为试验材料,采用Illumina Hi Seq/Mi Seq测序技术分离得到1个库尔勒香梨上的苹果茎沟病毒分离物ASGV-kfp的全长序列。序列分析结果显示:该分离物基因全长6 460 bp(ASGV-kfp,KR106996),包含3个完整的ORF,分别编码polyprotein、MP基因(Movement protein gene)、CP基因(Trichovirus coat protein gene)。ASGV-kfp的CP基因和MP基因与国内外已报道的该病毒的CP基因和MP基因序列同源性分别达到了93%~99%和97%~100%。进化树分析显示,ASGV的CP基因分子变异与寄主以及地理区域没有相关性;ASGV的MP基因分子变异与寄主有一定的相关性。研究结果可为进一步研究ASGV株系分化奠定良好基础。
文摘Based on growth level and peroxidase activity,a comparative study and paried-samples t test were made between virus-free Zaosu pear seedlings and the seedlings inoculated with apple stem grooving virus(ASGV). The results showed : (1) virus-free Zaosu pear seedlings grew better than inoculated ones. Of the seedlings the height,diameter,branches and autumn braches differed statistically significantly; (2)the peroxidase activity of inoculated seedlings was higher than that of virus-free ones,and the peak of peroxidase activity occurred on about d 7 and d 24 after inoculation. Fig 1,Tab 1,Ref
