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大蒜叶凝集素基因ASAL的克隆与表达载体的构建 被引量:3
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作者 陈德西 何忠全 +1 位作者 向运佳 胡荣平 《西南农业学报》 CSCD 北大核心 2016年第10期2445-2449,共5页
大蒜叶凝集素基因(Allium sativum leaf agglutinin,ASAL)是一种高活性的凝集素基因,且具显著的抗虫性。克隆大蒜ASAL基因将为其在转基因作物中抗虫特性研究奠定基础。本文以GenBank公布的ASAL基因序列设计特异引物,采用RT-PCR法从当地... 大蒜叶凝集素基因(Allium sativum leaf agglutinin,ASAL)是一种高活性的凝集素基因,且具显著的抗虫性。克隆大蒜ASAL基因将为其在转基因作物中抗虫特性研究奠定基础。本文以GenBank公布的ASAL基因序列设计特异引物,采用RT-PCR法从当地大蒜品种二水早和新都软叶的幼苗中克隆ASAL基因。结果表明,克隆的两个ASAL基因与已知基因的碱基序列相似性分别为98.7%和98.9%,氨基酸序列相似性为98.3%。将其亚克隆至表达载体中,经菌落PCR和酶切鉴定,成功构建了重组质粒。将该载体导入农杆菌EHA5a进行保存。 展开更多
关键词 ASAL基因 刺吸式害虫 基因克隆 表达载体 构建
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Trends of Extreme Temperature and Rainfall Indices for Arid and Semi-Arid Lands of South Eastern Kenya 被引量:3
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作者 Samwel N. Marigi Andrew K. Njogu William N. Githungo 《Journal of Geoscience and Environment Protection》 2016年第12期158-171,共14页
Extreme climate events have profound impacts on economies and livelihoods of many regions of the world. In Kenya, the extreme climate events often have strong impacts on agriculture production systems in the Arid and ... Extreme climate events have profound impacts on economies and livelihoods of many regions of the world. In Kenya, the extreme climate events often have strong impacts on agriculture production systems in the Arid and Semi-Arid Lands (ASALs). A small change in the mean climate condition can cause large changes in these production systems. There is a paucity of information on trends in climate and climate extremes in the country. However, a joint World Meteorological Commission for Climatology/World Climate Research Programme (WCPRP) project on climate Variability and Predictability (WMO CCl/CLIVAR) Expert Team (ET) on Climate Change Detection, Monitoring and Indices has defined 27 core climate indices mainly focusing on extreme events which can be derived through the use of RClimDex Software. In this study, therefore, the RClimDex software has been used to derive climate extreme indices for five stations in the ASALs of South-Eastern Kenya based on climate data for the period 1961 to 2009. The objective was to examine trends in these extremes to aid agricultural planning and practice. These indices have shown decreasing trends in annual rainfall, rainfall intensity and consecutive wet days but increasing trends in consecutive dry days. Steady warming patterns were evident in both the maximum and minimum temperature indices. This paper concludes that indeed significant changes in climate extremes are apparent in the ASALs of the country and recommends a re-thinking of planning and practice of rain-fed agriculture in the ASALs of South-Eastern Kenya. 展开更多
关键词 Extreme Climate Events Climate Indices Agriculture Systems asals RClimDex Soft Software
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Zai Pit Effects on Selected Soil Properties and Cowpea (<i>Vigna unguiculata</i>) Growth and Grain Yield in Two Selected Dryland Regions of Kenya
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作者 Sillus O. Oduor Nancy W. Mungai Seth F. O. Owido 《Open Journal of Soil Science》 2021年第1期39-57,共19页
Erratic rainfall and temperature regimes, strongly affect agricultural productivity. To address the reduction in production, this study assessed the effect of Zai pit depths on selected soil properties and cowpea grow... Erratic rainfall and temperature regimes, strongly affect agricultural productivity. To address the reduction in production, this study assessed the effect of Zai pit depths on selected soil properties and cowpea growth and grain yield. “Zai” pit technology was tested in two locations falling under Agroecological Zone IV (relatively dry areas) <i>i.e.</i> Katumani in Machakos County and Naivasha in Nakuru County, Kenya, aiming to determine the combined effect of four “Zai” pit depths and two levels of manure (plots with manure and plots without manure) on selected soil properties, growth and yield of cowpea. Experiment was laid out in split plot arrangement, with manure levels as the main plot factor and “Zai” pit depths (Flat: Z<sub>0</sub>, 30 cm: Z<sub>30</sub>, 45 cm: Z<sub>45</sub> and 60 cm: Z<sub>60</sub>) as subplot factor, replicated four times. Cowpea (M66 variety) was used as the test crop. Inorganic N and extractable P were significantly (P < 0.05) higher, at 1.37 mg<span style="white-space:nowrap;"><span style="white-space:nowrap;">&sdot;</span></span>kg<sup><span style="white-space:nowrap;"><span style="white-space:nowrap;">&minus;</span></span>1</sup> for N<sub>in</sub> and 80.4 mg<span style="white-space:nowrap;"><span style="white-space:nowrap;">&sdot;</span></span>kg<sup><span style="white-space:nowrap;"><span style="white-space:nowrap;">&minus;</span></span>1</sup> for P<sub>ex</sub> in Zai pits compared to flat plots which were at 0.91 mg<span style="white-space:nowrap;"><span style="white-space:nowrap;">&sdot;</span></span>kg<sup><span style="white-space:nowrap;"><span style="white-space:nowrap;">&minus;</span></span>1</sup> for N<sub>in</sub> and 47.1 mg<span style="white-space:nowrap;"><span style="white-space:nowrap;">&sdot;</span></span>kg<sup><span style="white-space:nowrap;"><span style="white-space:nowrap;">&minus;</span></span>1</sup> for P<sub>ex</sub>. The values of N<sub>in</sub> and P<sub>ex</sub> also varied depending on depths, with Z<sub>45</sub> having highest N<sub>in</sub> at 1.17 against the least, at 0.89 in the Z<sub>0</sub>, while Pex was highest in Z<sub>30</sub> at 102.3 mg<span style="white-space:nowrap;"><span style="white-space:nowrap;">&sdot;</span></span>kg<sup><span style="white-space:nowrap;"><span style="white-space:nowrap;">&minus;</span></span>1</sup> while Z<sub>0</sub> having the least P<sub>ex</sub> of 89.7 mg<span style="white-space:nowrap;"><span style="white-space:nowrap;">&sdot;</span></span>kg<sup><span style="white-space:nowrap;"><span style="white-space:nowrap;">&minus;</span></span>1</sup>. Generally, crops in “Zai” pitted plots were larger in diameter at 0.46 cm than crops in flat plots at 0.42 cm. Better performance was observed in yield, with Z<sub>30</sub> yielding 30.5% against 18.2% Flat plots in Machakos while 27.9% in Z<sub>30</sub> against 22.5% from Flat plots in Naivasha. This study demonstrated great potential of “Zai” pit technology on crop production, as reflected on improved growth and yield of cowpeas. Combining “Zai” pits with manure increases soil N<sub>in</sub>, P<sub>ex</sub> and is a guarantee of great crop performance in terms of high final yields. 展开更多
关键词 Zai Pits asals Soil Moisture Dryland Agriculture Cattle Manure Inorganic Nitrogen Extractable Phosphorus
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防止ADSL数据对话音的干扰
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作者 柳华栋 《电信网技术》 2003年第1期54-56,共3页
介绍了ADSL的数据信道与话音信道以及ADSL的频带衰减,并对防止数据信号干扰电话信号进行了分析研究。
关键词 ADSL 数据信道 话音信道 分离器 ASAL 频带衰减
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Climate Change and Pastoral Economy in Kenya:A Blinking Future
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作者 Julius M.Huho Josephine K.W.Ngaira Harun O.Ogindo 《地学前缘》 EI CAS CSCD 北大核心 2009年第S1期107-107,共1页
This paper examines the changing climatic scenarios and the associated effects on livestock farming in the arid lands(ASALs)of Kenya which covers over 80%of the country.This study was carried out in the semi-arid Muko... This paper examines the changing climatic scenarios and the associated effects on livestock farming in the arid lands(ASALs)of Kenya which covers over 80%of the country.This study was carried out in the semi-arid Mukogodo Division of Laikipia District.This division received a mean annual rainfall of about 507.8 mm and the main source of livelihood was livestock farming.Questionnaire, structured interview,observation and literature 展开更多
关键词 Kenya ASAL pastoralism CLIMATE change DROUGHT
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Insight to the Mode of Action of <i>Allium sativum</i>Leaf Agglutinin (ASAL) Expressing in T<sub>3</sub>Rice Lines on Brown Planthopper
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作者 Arpita Bala Amit Roy +2 位作者 Niranjana Behura Daniel Hess Sampa Das 《American Journal of Plant Sciences》 2013年第2期400-407,共8页
Brown planthopper, the sap sucking hemipteran pest, is one of the major contributors to the yield loss of rice through the world. To combat the situation researchers are interested identifying genes from plant origin ... Brown planthopper, the sap sucking hemipteran pest, is one of the major contributors to the yield loss of rice through the world. To combat the situation researchers are interested identifying genes from plant origin having potentiality to develop hemipteran pest resistance. Interestingly, it was observed that rice plants expressing ASAL, a monocot mannose binding lectin, showed significant resistance to brown planthopper and green leafhopper. Additionally, antibiotic resistant marker gene free ASAL expressing rice lines were developed to overcome the biosafety issues. However, the basis behind the resistance against planthoppers is still not clearly understood. Ligand blot assay was performed with total BBMV protein from BPH and a ~56 kDa receptor protein was detected. LC MS/MS analysis revealed that the receptor protein is NADH quinone oxidoreductase (NQO), a key player in electron transport chain, insect defense response and male/female gametogenesis. Presumably interaction of ASAL with NQO may lead to toxicity and loss of fecundity among BPH feeding on ASAL expressing transgenic rice plants. These findings provide a stable scientific basis for considering these transgenic ASAL expressing rice plants as significant product for combating BPH attack associated yield loss of rice. 展开更多
关键词 Allium sativum LEAF AGGLUTININ (ASAL) Hemipteran Pest Management Brown Planthopper NADH Qui-none Oxidoreductase (NQO) Fecundity Transgenic RICE Brush BOARDER Membrane Vesicle (BBMV) Protein
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