【目的】实验室前期筛选得到能够分泌具有抑菌作用挥发性物质的5株芽胞杆菌菌株,进一步明确生防芽胞杆菌挥发性物质的促生功能,并鉴定其挥发性活性组分,以期获得具有抑菌和促生双重功能的优良菌株,研发具有促生作用的挥发性天然产物。...【目的】实验室前期筛选得到能够分泌具有抑菌作用挥发性物质的5株芽胞杆菌菌株,进一步明确生防芽胞杆菌挥发性物质的促生功能,并鉴定其挥发性活性组分,以期获得具有抑菌和促生双重功能的优良菌株,研发具有促生作用的挥发性天然产物。【方法】采用盆栽熏蒸法、选择培养基测定和熵值计算法综合评定出最佳促生菌株。通过高效液相色谱技术检测其分泌的挥发性物质对马铃薯中细胞分裂素、赤霉素和生长素含量的影响,并采用实时荧光定量PCR分析挥发性物质对马铃薯内源激素相关合成基因(StCKX1、StLAX1和StGA2ox1)表达量的影响。通过全二维气相色谱-质谱(two-dimensional gas chromatography mass spectrometry,GC×GC-MS)对菌株挥发性成分进行检测,利用NIST/EPA/NIH数据库鉴定成分。根据峰面积比例,购买相对含量最高的3种纯品,体外检测其对植株的促生作用。【结果】贝莱斯芽胞杆菌NZ-4分泌的挥发性物质熏蒸后马铃薯促生作用最为显著,植株株高和根长分别增加了40.01%和15.15%;NZ-4具有溶磷、固氮和产生铁载体的能力;熵值法综合评价结果中NZ-4菌株得分最高,为0.9476。因此,确定NZ-4为最优促生菌株。经贝莱斯芽胞杆菌NZ-4挥发性物质熏蒸后,马铃薯叶片中细胞分裂素、赤霉素和生长素的含量较对照组分别增加了1.09倍、0.87倍和1.89倍,StGA20ox1和StLAX1基因的表达量较对照提升了3.61倍和4.71倍。GC×GC-MS结果表明,2-庚酮、3-乙基甲苯和2-壬酮相对含量最高;浓度为1μg/μL的2-庚酮和100 ng/μL的3-乙基甲苯能够显著促进植株的生长,2-壬酮对植株的生长没有明显作用。【结论】贝莱斯芽胞杆菌NZ-4产生的挥发性物质对马铃薯具有良好的促生作用,明确了2-庚酮和3-乙基甲苯为挥发性活性成分,NZ-4菌株产生的挥发性有机物具有开发新型天然产物的潜力。展开更多
The electrical resistivity of NZ30K-Mg alloy was measured at different heating rates during continuous heating to stud the precipitation kinetics.Two kinds of metastable phases,β＂ and β＇,formed during the heating....The electrical resistivity of NZ30K-Mg alloy was measured at different heating rates during continuous heating to stud the precipitation kinetics.Two kinds of metastable phases,β＂ and β＇,formed during the heating.Kissinger method and differentia isoconversional method were employed to assess the precipitation kinetic parameters of NZ30K-Mg alloy,activation energy Eα an pre-exponential factor A＇α.The fraction of transformation（α） and the precipitation sequence in NZ30K-Mg alloy were determinec Continuous heating transformation（CHT） and isothermal heating transformation（IHT） diagrams were further obtained for guidin the aging of NZ30K-Mg alloy.The analysis shows that the precipitation kinetic parameters of NZ30K-Mg alloy can be obtaine accurately using isoconversional method.展开更多
文摘【目的】实验室前期筛选得到能够分泌具有抑菌作用挥发性物质的5株芽胞杆菌菌株,进一步明确生防芽胞杆菌挥发性物质的促生功能,并鉴定其挥发性活性组分,以期获得具有抑菌和促生双重功能的优良菌株,研发具有促生作用的挥发性天然产物。【方法】采用盆栽熏蒸法、选择培养基测定和熵值计算法综合评定出最佳促生菌株。通过高效液相色谱技术检测其分泌的挥发性物质对马铃薯中细胞分裂素、赤霉素和生长素含量的影响,并采用实时荧光定量PCR分析挥发性物质对马铃薯内源激素相关合成基因(StCKX1、StLAX1和StGA2ox1)表达量的影响。通过全二维气相色谱-质谱(two-dimensional gas chromatography mass spectrometry,GC×GC-MS)对菌株挥发性成分进行检测,利用NIST/EPA/NIH数据库鉴定成分。根据峰面积比例,购买相对含量最高的3种纯品,体外检测其对植株的促生作用。【结果】贝莱斯芽胞杆菌NZ-4分泌的挥发性物质熏蒸后马铃薯促生作用最为显著,植株株高和根长分别增加了40.01%和15.15%;NZ-4具有溶磷、固氮和产生铁载体的能力;熵值法综合评价结果中NZ-4菌株得分最高,为0.9476。因此,确定NZ-4为最优促生菌株。经贝莱斯芽胞杆菌NZ-4挥发性物质熏蒸后,马铃薯叶片中细胞分裂素、赤霉素和生长素的含量较对照组分别增加了1.09倍、0.87倍和1.89倍,StGA20ox1和StLAX1基因的表达量较对照提升了3.61倍和4.71倍。GC×GC-MS结果表明,2-庚酮、3-乙基甲苯和2-壬酮相对含量最高;浓度为1μg/μL的2-庚酮和100 ng/μL的3-乙基甲苯能够显著促进植株的生长,2-壬酮对植株的生长没有明显作用。【结论】贝莱斯芽胞杆菌NZ-4产生的挥发性物质对马铃薯具有良好的促生作用,明确了2-庚酮和3-乙基甲苯为挥发性活性成分,NZ-4菌株产生的挥发性有机物具有开发新型天然产物的潜力。
基金Project (2011ZX04014-052,2012ZX04012011) supported by CNC Machine Tools and Basic Manufacturing Equipment Technology Comments
文摘The electrical resistivity of NZ30K-Mg alloy was measured at different heating rates during continuous heating to stud the precipitation kinetics.Two kinds of metastable phases,β＂ and β＇,formed during the heating.Kissinger method and differentia isoconversional method were employed to assess the precipitation kinetic parameters of NZ30K-Mg alloy,activation energy Eα an pre-exponential factor A＇α.The fraction of transformation（α） and the precipitation sequence in NZ30K-Mg alloy were determinec Continuous heating transformation（CHT） and isothermal heating transformation（IHT） diagrams were further obtained for guidin the aging of NZ30K-Mg alloy.The analysis shows that the precipitation kinetic parameters of NZ30K-Mg alloy can be obtaine accurately using isoconversional method.
