目的:分析大鼠小脑颗粒神经元(CGNs)“低钾”性凋亡过程中Arnt2亚细胞定位的变化。方法:W estern b lotting法分析“低钾”性凋亡过程中CGNs核抽提物中Arnt2蛋白质的表达变化,间接免疫荧光结合激光扫描共聚焦显微术(LSM)分析Arnt2蛋白...目的:分析大鼠小脑颗粒神经元(CGNs)“低钾”性凋亡过程中Arnt2亚细胞定位的变化。方法:W estern b lotting法分析“低钾”性凋亡过程中CGNs核抽提物中Arnt2蛋白质的表达变化,间接免疫荧光结合激光扫描共聚焦显微术(LSM)分析Arnt2蛋白质亚细胞定位的变化。结果:在CGNs细胞核抽提物中,“低钾”诱导30 m in后Arnt2蛋白质已明显表达上调,1 h后达至峰值水平,“低钾”诱导6 h后上调表达的Arnt2回落至正常对照水平;LSM分析显示位于正常CGNs细胞核内的Arnt2在“低钾”作用下逐步向胞浆转移,并在CGNs凋亡末期与胞浆线粒体定位的HSP60完全重叠。结论:Arnt2在小脑颗粒神经元“低钾”性凋亡过程中发生了核浆转位;结果提示,Arnt2很可能是通过传递“低钾”诱发的细胞凋亡或存活信号而直接参与小脑颗粒神经元“低钾”性凋亡的调控。展开更多
To evaluate the protective effects of Glycyrrhiza polysaccharide（GPS） against 2,3,7,8-tetrachlorodibenzo-p-dioxin（TCDD）-induced hepatotoxicity in Jian carp,the fish were fed diets containing GPS at doses of 0.1,0....To evaluate the protective effects of Glycyrrhiza polysaccharide（GPS） against 2,3,7,8-tetrachlorodibenzo-p-dioxin（TCDD）-induced hepatotoxicity in Jian carp,the fish were fed diets containing GPS at doses of 0.1,0.5 and 1.0 g/kg for 60 days before an intraperitoneal injection of 0.6 μg/kg TCDD at a volume of 0.05 mL/10 g body weight.At 72 hr post-injection,blood and liver samples were taken for biochemical analysis and the fish liver samples were used for the preparation of pathological slices.The results showed that increases in alanine aminotransferase（GPT）,aspartate aminotransferase（GOT）,lactate dehydrogenase（LDH）,and alkaline phosphatase（AKP） in serum induced by TCDD were significantly inhibited by pre-treatment with 1.0 g/kg GPS.Following the 1.0 g/kg GPS pre-treatment,total protein（TP）,albumin（Alb）,catalase（CAT）,glutathione peroxidase（GPx）,total antioxidant capacity（T-AOC） and superoxide dismutase（SOD） activities in liver tissue increased significantly,malondialdehyde（MDA） formation（P ＆lt; 0.05 or P ＆lt; 0.01） was significantly inhibited,and the expression of cytochrome P4501A（CYP1A）,aryl hydrocarbon receptor 2（AHR2） and aryl hydrocarbon receptor nuclear translocator 2（ARNT2） mRNA（P ＆lt; 0.05） was significantly enhanced.Histological observations on fish liver were obtained by preparing paraffin tissue sections via HE staining,and the results showed that histological changes were obviously reduced by 0.5 and 1.0 g/kg GPS.GPS significantly reduced liver tissue damage caused by TCDD.Overall,these results proved the hepatoprotective effect of GPS in protecting against fish liver injury induced by TCDD,and supported the use of GPS（1.0 g/kg） as a hepatoprotective and antioxidant agent in fish.展开更多
文摘目的:分析大鼠小脑颗粒神经元(CGNs)“低钾”性凋亡过程中Arnt2亚细胞定位的变化。方法:W estern b lotting法分析“低钾”性凋亡过程中CGNs核抽提物中Arnt2蛋白质的表达变化,间接免疫荧光结合激光扫描共聚焦显微术(LSM)分析Arnt2蛋白质亚细胞定位的变化。结果:在CGNs细胞核抽提物中,“低钾”诱导30 m in后Arnt2蛋白质已明显表达上调,1 h后达至峰值水平,“低钾”诱导6 h后上调表达的Arnt2回落至正常对照水平;LSM分析显示位于正常CGNs细胞核内的Arnt2在“低钾”作用下逐步向胞浆转移,并在CGNs凋亡末期与胞浆线粒体定位的HSP60完全重叠。结论:Arnt2在小脑颗粒神经元“低钾”性凋亡过程中发生了核浆转位;结果提示,Arnt2很可能是通过传递“低钾”诱发的细胞凋亡或存活信号而直接参与小脑颗粒神经元“低钾”性凋亡的调控。
基金supported by the National Natural Science Foundation of China(Nos.31202002,31200918)Jiangsu Science and Technology Department(No.BK2012535)the Central Public-Interest Scientific Institution Basal Research Fund(No.2014A08YQ01)
文摘To evaluate the protective effects of Glycyrrhiza polysaccharide（GPS） against 2,3,7,8-tetrachlorodibenzo-p-dioxin（TCDD）-induced hepatotoxicity in Jian carp,the fish were fed diets containing GPS at doses of 0.1,0.5 and 1.0 g/kg for 60 days before an intraperitoneal injection of 0.6 μg/kg TCDD at a volume of 0.05 mL/10 g body weight.At 72 hr post-injection,blood and liver samples were taken for biochemical analysis and the fish liver samples were used for the preparation of pathological slices.The results showed that increases in alanine aminotransferase（GPT）,aspartate aminotransferase（GOT）,lactate dehydrogenase（LDH）,and alkaline phosphatase（AKP） in serum induced by TCDD were significantly inhibited by pre-treatment with 1.0 g/kg GPS.Following the 1.0 g/kg GPS pre-treatment,total protein（TP）,albumin（Alb）,catalase（CAT）,glutathione peroxidase（GPx）,total antioxidant capacity（T-AOC） and superoxide dismutase（SOD） activities in liver tissue increased significantly,malondialdehyde（MDA） formation（P ＆lt; 0.05 or P ＆lt; 0.01） was significantly inhibited,and the expression of cytochrome P4501A（CYP1A）,aryl hydrocarbon receptor 2（AHR2） and aryl hydrocarbon receptor nuclear translocator 2（ARNT2） mRNA（P ＆lt; 0.05） was significantly enhanced.Histological observations on fish liver were obtained by preparing paraffin tissue sections via HE staining,and the results showed that histological changes were obviously reduced by 0.5 and 1.0 g/kg GPS.GPS significantly reduced liver tissue damage caused by TCDD.Overall,these results proved the hepatoprotective effect of GPS in protecting against fish liver injury induced by TCDD,and supported the use of GPS（1.0 g/kg） as a hepatoprotective and antioxidant agent in fish.
