Stroke patients experience varying degrees of upper limb functional impairment.Although bilateral arm training can help stroke patients recover movement after stroke,little is known about the way in which the brain an...Stroke patients experience varying degrees of upper limb functional impairment.Although bilateral arm training can help stroke patients recover movement after stroke,little is known about the way in which the brain and muscles work together during this type of training.To address this,we conducted a cross-sectional study at The Seventh Affiliated Hospital,Sun Yat-sen University in China,where we observed the connection between brain and muscle activity during bilateral upper limb training in 21 stroke patients and 17 healthy controls.We used functional near-infrared spectroscopy and surface electromyography to measure changes in cerebral cortex oxygenation and upper limb muscle contraction signals,respectively.The results showed that,compared with the healthy control group,stroke patients had reduced functional connectivity and more irregular muscle activity in the affected flexor muscle during bilateral upper limb training.Moreover,we found a significant correlation between the surface electromyographic signal characteristics of upper limb muscles and cerebral oxygenation indicators of multiple brain regions in stroke patients.These findings indicate that bilateral upper limb training is an effective rehabilitation method that improves upper limb motor function in stroke patients by promoting brain functional connectivity and improving muscle activity patterns.展开更多
文摘【目的】早发性肌无力综合征(early onset muscle weakness syndrome,MW)是新近发现的一种荷斯坦牛遗传缺陷,患病犊牛表现为出生后趴卧不起、后肢肌肉萎缩等,其遗传机制与L型钙通道蛋白α1S亚基编码基因CACNA1S的单碱基突变相关。本研究旨在建立该遗传缺陷的分子检测方法,并探究其在国内荷斯坦牛群体中的扩散情况。【方法】基于MW致病位点特异性DNA序列,设计扩增阻滞突变系统-聚合酶链式反应(ARMS-PCR)检测引物,对317份荷斯坦牛冻精和毛囊样本进行MW遗传缺陷基因筛查,通过Sanger测序对ARMS-PCR检测结果进行验证,并利用系谱数据追溯MW突变源头。【结果】ARMS-PCR检出MW携带者21头,携带率为6.62%(21/317),未发现缺陷基因纯合子,提示该突变为隐性纯合致死。Sanger测序与ARMS-PCR所得基因型完全一致,证明该技术具有高度准确性。系谱追溯表明,MW携带者的遗传来源可追溯至1984年出生的荷斯坦公牛Southwind Bell of Bar-Lee。【结论】MW遗传缺陷已在中国荷斯坦牛群体中扩散且携带率较高,建议牧场应尽早开展MW遗传缺陷基因检测和风险评估,采取科学的选种选配措施以减少遗传缺陷导致的经济损失。
文摘Stroke patients experience varying degrees of upper limb functional impairment.Although bilateral arm training can help stroke patients recover movement after stroke,little is known about the way in which the brain and muscles work together during this type of training.To address this,we conducted a cross-sectional study at The Seventh Affiliated Hospital,Sun Yat-sen University in China,where we observed the connection between brain and muscle activity during bilateral upper limb training in 21 stroke patients and 17 healthy controls.We used functional near-infrared spectroscopy and surface electromyography to measure changes in cerebral cortex oxygenation and upper limb muscle contraction signals,respectively.The results showed that,compared with the healthy control group,stroke patients had reduced functional connectivity and more irregular muscle activity in the affected flexor muscle during bilateral upper limb training.Moreover,we found a significant correlation between the surface electromyographic signal characteristics of upper limb muscles and cerebral oxygenation indicators of multiple brain regions in stroke patients.These findings indicate that bilateral upper limb training is an effective rehabilitation method that improves upper limb motor function in stroke patients by promoting brain functional connectivity and improving muscle activity patterns.
