Chemical constituents of the whole herb of Saruma henryi Oliv. were investigated. The herbal extract was separated by repeated column chromatography over silica gel and celite. The structures were elucidated by spectr...Chemical constituents of the whole herb of Saruma henryi Oliv. were investigated. The herbal extract was separated by repeated column chromatography over silica gel and celite. The structures were elucidated by spectroscopic analysis. Thirteen compounds were obtained and identified as 7-methoxyl-aristololactam Ⅳ （1）, aristololactam Ⅱ （2）, aristolochic acid Ⅰ （3）, aristololactam AⅡ （4）, daucosterol （5）, aristololactam Ⅰa （6）, N-trans-feruloyl tyramine （7）, aristololactam Ⅰ （8）, 4β,10β-aromadendranediol （9）, aristololide （10）, aristolic acid Ⅰ （11）, meso-dihydroguaiaretic acid （12）, and calopiptin （13）. These compounds were obtained from the genus Saruma for the first time, and they provided chemical evidences for the chemotaxonomy of plants of the Aristolochiaceae family. Since aristolochic acids and aristololactams are toxic to kidney, the results of this investigation suggest that it should be cautious to use Saruma henryi as a medicine.展开更多
Objective:To evaluate a protective effect of Aristolochia gehrtii(A.gehrtii)leaves to inhibit liver toxicity and apoptosis in Schistosoma malayensis(S.malayensis)infection.Methods:Forty male albino mice were divided i...Objective:To evaluate a protective effect of Aristolochia gehrtii(A.gehrtii)leaves to inhibit liver toxicity and apoptosis in Schistosoma malayensis(S.malayensis)infection.Methods:Forty male albino mice were divided into four equal groups:group 1 control including noninfected healthy mice and groups 2,3&4 subcutaneously infected with S.malayensvs cercariae where groups 3&4 pretreated with A.gehrtii leaves(200 mg/kg,bwt)&cinnamoylamide(250mg/kg,bwt),respectively.Results:5.malayensis caused a significant increase in serum AST,ALT,ALP,MDA,NO,bilirubin,urea,creatinine,total cholesterol,LDL,triglycerides,and HDL levels.The pretreatment of A,gehrtii leaves and cinnamoylamide significantly inhibited that increase.On the other hand,S.malayensis induced a significant decrease in serum total protein,albumin,globulin,albumin/globulin ratio,blood SOD and GPx,while A.gehrtii leaves and cinnamoylamide pretreatment increased the above parameters.Treatment with A.gehrtii leaves and cinnamoylamide to S.malayensis infected mice increased p53 expression but decreased bcl-2expression.These results were supported by hislopalholqgical investigations.Conclusions:A.gehrtii inhibits liver toxicity and apoptosis in S.malayensvs infection and this effect is associated with the major cinnamoylamide ingredient of A.gehrtii leaves.展开更多
Aristolochic acids(AAs)are an important group of secondary metabolites in the genus Aristolochia.The presence of aristolochic acids infers the potency of many Aristolochia herbs used forages in traditional medicine of...Aristolochic acids(AAs)are an important group of secondary metabolites in the genus Aristolochia.The presence of aristolochic acids infers the potency of many Aristolochia herbs used forages in traditional medicine of China,Europe,Central America,India,and some other countries.Although being moderately cytotoxic,intake of AAs is asso-ciated with serious health problems,such as nephrotoxicity and carcinogenicity.Analyzing AAs in Aristolochia herbs is crucial for regulating their efficacy and toxicity because phytochemistry works have shown the occurrence of AAs in almost all Aristolochia herbs studied.Using two-dimensional parameters,chemical shifts and coupling constants,NMR spectroscopy is a modern,accurate,and reliable method in the analysis of secondary metabolites.Compar-ing experimental spectroscopic data with those of known and related compounds helps simplify the structural identification of secondary metabolites.The compilation of an NMR database of AAs from scattered sources would also be useful in NMR-based metabolomics.The present review provides updated information on sources and NMR spectroscopic data of 54 aristolochic acid derivatives,including AAs and their methyl esters,denitroaristolochic acids and their derivatives,and sesqui-and diterpene esters of AAs.The report also covers the newest development of ana-lytical and preparative methods used in separation,identification,and quantification of AAs in Aristolochia herbal samples.展开更多
基金National Natural Science Foundation of China (Grant No. 30371748)the 985 Project of Peking University and the National Eleventh Five-year Key Technologies R & D Program of China (Grant No. 2006BAI14B01)
文摘Chemical constituents of the whole herb of Saruma henryi Oliv. were investigated. The herbal extract was separated by repeated column chromatography over silica gel and celite. The structures were elucidated by spectroscopic analysis. Thirteen compounds were obtained and identified as 7-methoxyl-aristololactam Ⅳ （1）, aristololactam Ⅱ （2）, aristolochic acid Ⅰ （3）, aristololactam AⅡ （4）, daucosterol （5）, aristololactam Ⅰa （6）, N-trans-feruloyl tyramine （7）, aristololactam Ⅰ （8）, 4β,10β-aromadendranediol （9）, aristololide （10）, aristolic acid Ⅰ （11）, meso-dihydroguaiaretic acid （12）, and calopiptin （13）. These compounds were obtained from the genus Saruma for the first time, and they provided chemical evidences for the chemotaxonomy of plants of the Aristolochiaceae family. Since aristolochic acids and aristololactams are toxic to kidney, the results of this investigation suggest that it should be cautious to use Saruma henryi as a medicine.
文摘Objective:To evaluate a protective effect of Aristolochia gehrtii(A.gehrtii)leaves to inhibit liver toxicity and apoptosis in Schistosoma malayensis(S.malayensis)infection.Methods:Forty male albino mice were divided into four equal groups:group 1 control including noninfected healthy mice and groups 2,3&4 subcutaneously infected with S.malayensvs cercariae where groups 3&4 pretreated with A.gehrtii leaves(200 mg/kg,bwt)&cinnamoylamide(250mg/kg,bwt),respectively.Results:5.malayensis caused a significant increase in serum AST,ALT,ALP,MDA,NO,bilirubin,urea,creatinine,total cholesterol,LDL,triglycerides,and HDL levels.The pretreatment of A,gehrtii leaves and cinnamoylamide significantly inhibited that increase.On the other hand,S.malayensis induced a significant decrease in serum total protein,albumin,globulin,albumin/globulin ratio,blood SOD and GPx,while A.gehrtii leaves and cinnamoylamide pretreatment increased the above parameters.Treatment with A.gehrtii leaves and cinnamoylamide to S.malayensis infected mice increased p53 expression but decreased bcl-2expression.These results were supported by hislopalholqgical investigations.Conclusions:A.gehrtii inhibits liver toxicity and apoptosis in S.malayensvs infection and this effect is associated with the major cinnamoylamide ingredient of A.gehrtii leaves.
基金funded by Vietnam National Foundation for Science and Technology Development(NAFOSTED)under Grant Number 104.01-2021.09
文摘Aristolochic acids(AAs)are an important group of secondary metabolites in the genus Aristolochia.The presence of aristolochic acids infers the potency of many Aristolochia herbs used forages in traditional medicine of China,Europe,Central America,India,and some other countries.Although being moderately cytotoxic,intake of AAs is asso-ciated with serious health problems,such as nephrotoxicity and carcinogenicity.Analyzing AAs in Aristolochia herbs is crucial for regulating their efficacy and toxicity because phytochemistry works have shown the occurrence of AAs in almost all Aristolochia herbs studied.Using two-dimensional parameters,chemical shifts and coupling constants,NMR spectroscopy is a modern,accurate,and reliable method in the analysis of secondary metabolites.Compar-ing experimental spectroscopic data with those of known and related compounds helps simplify the structural identification of secondary metabolites.The compilation of an NMR database of AAs from scattered sources would also be useful in NMR-based metabolomics.The present review provides updated information on sources and NMR spectroscopic data of 54 aristolochic acid derivatives,including AAs and their methyl esters,denitroaristolochic acids and their derivatives,and sesqui-and diterpene esters of AAs.The report also covers the newest development of ana-lytical and preparative methods used in separation,identification,and quantification of AAs in Aristolochia herbal samples.
