[Objective]This study aimed to investigate the allelopathic effects of Torreya fargesii aril.[Method]By indoor bioassay,the effects of different con-centrations(10,20,40,80 g/L)of aqueous extract of T.fargesii aril on...[Objective]This study aimed to investigate the allelopathic effects of Torreya fargesii aril.[Method]By indoor bioassay,the effects of different con-centrations(10,20,40,80 g/L)of aqueous extract of T.fargesii aril on seed germination rate,seedling root length,seedling height,fresh weight,chlorophyll content,malondialdehyde(MDA)content and anti oxidative enzyme activities of radish,mustard and cabbage were analyzed and compared.[Result]Different concentrations of aqueous extract of T.fargesii aril inhibited seed germination and seedling growth of three crops,and the inhibitory effects were enhanced with the increase of concentration.Seedling height and fresh weight of three crops were improved by low concentrations of aqueous extract of T.fargesii aril and inhibited by high concentrations of aqueous extract.Overall,aqueous extract of T.fargesii aril exhibited allelopathic inhibitory effects on three crops,and the level of allelopath-ic inhibitory effects demonstrated a descending order of radish〉mustard〉cabbage.Compared with the control group,80 g/L aqueous extract of T.fargesii aril almost significantly reduced seed germination rate,seedling root length,seedling height,fresh weight and chlorophyll content,and significantly improved MDA con-tent and antioxidative enzyme(SOD,CAT,POD)activities of radish,mustard and cabbage(P〈0.05).[Conclusion]Aqueous extract of T.fargesii aril exhibited remarkable allelopathic inhibitory effects on seed germination of radish,mustard and cabbage,which indicated that there might be dormancy-associated germination in-hibiting allelochemicals in T.fargesii aril.This study provided theoretical basis for subsequent clarification of the mechanism of seed dormancy of T.fargesii.展开更多
[Objectives]To explore the neuroprotective effects and mechanism of Longan Aril(LA)effective parts on PC12 cells injured by H2O2.[Methods]The neuroprotective effects of LA were evaluated by the cell viability,SOD and ...[Objectives]To explore the neuroprotective effects and mechanism of Longan Aril(LA)effective parts on PC12 cells injured by H2O2.[Methods]The neuroprotective effects of LA were evaluated by the cell viability,SOD and MDA content,apoptosis assay and relative protein expression of Aβand p-Tau.The neuroprotective mechanism of LA was studied by using metabolomics and network pharmacology,and the expressions of RAS/MEK/ERK signaling pathway-related proteins were detected by western blotting.[Results]LA could improve the cell survival rate and SOD content,and reduce apoptosis and expression of Aβand p-tau.Inhibition of RAS/MEK/ERK signaling pathway is a possible mechanism of LA neuroprotective effects.[Conclusions]LA has a neuroprotective effects in vitro and be likely to inhibit the process of AD by inhibition of RAS/MEK/ERK signalling pathway.展开更多
This study examined the freshness of minimally processed pomegranate arils(MPPAs)under refrigeration conditions(5±1◦C)stored in a polypropylene(PP)container sealed with PP nanocomposite multi-layered film(PP-NMF)...This study examined the freshness of minimally processed pomegranate arils(MPPAs)under refrigeration conditions(5±1◦C)stored in a polypropylene(PP)container sealed with PP nanocomposite multi-layered film(PP-NMF).The freshness indicator was fabricated using cellulose acetate coated with a combination of colorimetric pH dyes,which included bromocresol green(BCG),bromothymol blue(BTB)and methyl red(MR)in a ratio of 6:9:15.The fabricated indicator labels were attached inside the package headspace and observed an increase in CO_(2)(0.1±0.01 to 0.9±0.03%v/v)and a decrease in O_(2)(19.6±0.01 to 18.8±0.03%v/v)during storage(0-18 days).Color change of indicator labels was mainly caused by headspace concentrations that changed from dark to colorless for fresh and spoiled pomegranate arils.The PP-NMF used in MPPAs has high barrier properties,extending its shelf life in the refrigerator to over 15 days.The pomegranate arils stored for different periods of time showed significant changes in their physicochemical parameters.Over storage days,aerobic mesophilic bacteria(AMB)and yeast and mold count(YMC)increased to 4.90±0.20 and 4.50±0.20 log CFU g1.An indicator developed for monitoring pomegranate arils stored in the refrigerator can detect freshness efficiently.展开更多
基金Supported by National Natural Science Foundation of China(31470568)Scientific and Technological Research Program of Chongqing Municipal Education Commission(KJ1401204)Program for Innovation Team Building of Yangtze Normal University(2014XJTD06)
文摘[Objective]This study aimed to investigate the allelopathic effects of Torreya fargesii aril.[Method]By indoor bioassay,the effects of different con-centrations(10,20,40,80 g/L)of aqueous extract of T.fargesii aril on seed germination rate,seedling root length,seedling height,fresh weight,chlorophyll content,malondialdehyde(MDA)content and anti oxidative enzyme activities of radish,mustard and cabbage were analyzed and compared.[Result]Different concentrations of aqueous extract of T.fargesii aril inhibited seed germination and seedling growth of three crops,and the inhibitory effects were enhanced with the increase of concentration.Seedling height and fresh weight of three crops were improved by low concentrations of aqueous extract of T.fargesii aril and inhibited by high concentrations of aqueous extract.Overall,aqueous extract of T.fargesii aril exhibited allelopathic inhibitory effects on three crops,and the level of allelopath-ic inhibitory effects demonstrated a descending order of radish〉mustard〉cabbage.Compared with the control group,80 g/L aqueous extract of T.fargesii aril almost significantly reduced seed germination rate,seedling root length,seedling height,fresh weight and chlorophyll content,and significantly improved MDA con-tent and antioxidative enzyme(SOD,CAT,POD)activities of radish,mustard and cabbage(P〈0.05).[Conclusion]Aqueous extract of T.fargesii aril exhibited remarkable allelopathic inhibitory effects on seed germination of radish,mustard and cabbage,which indicated that there might be dormancy-associated germination in-hibiting allelochemicals in T.fargesii aril.This study provided theoretical basis for subsequent clarification of the mechanism of seed dormancy of T.fargesii.
基金Liaoning Natural Science Foundation(20180530033)Open Fund of Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications,Liaoning University of Traditional Chinese Medicine。
文摘[Objectives]To explore the neuroprotective effects and mechanism of Longan Aril(LA)effective parts on PC12 cells injured by H2O2.[Methods]The neuroprotective effects of LA were evaluated by the cell viability,SOD and MDA content,apoptosis assay and relative protein expression of Aβand p-Tau.The neuroprotective mechanism of LA was studied by using metabolomics and network pharmacology,and the expressions of RAS/MEK/ERK signaling pathway-related proteins were detected by western blotting.[Results]LA could improve the cell survival rate and SOD content,and reduce apoptosis and expression of Aβand p-tau.Inhibition of RAS/MEK/ERK signaling pathway is a possible mechanism of LA neuroprotective effects.[Conclusions]LA has a neuroprotective effects in vitro and be likely to inhibit the process of AD by inhibition of RAS/MEK/ERK signalling pathway.
基金Financial assistance provided by the National Institute of Food Technology,Entrepreneurship and Management-Thanjavur(NIFTEM-T),Formerly Indian Institute of Food Processing Technology(IIFPT),Ministry of Food Processing Industries(MOFPI),Government of India(GOI),which is duly acknowledged.
文摘This study examined the freshness of minimally processed pomegranate arils(MPPAs)under refrigeration conditions(5±1◦C)stored in a polypropylene(PP)container sealed with PP nanocomposite multi-layered film(PP-NMF).The freshness indicator was fabricated using cellulose acetate coated with a combination of colorimetric pH dyes,which included bromocresol green(BCG),bromothymol blue(BTB)and methyl red(MR)in a ratio of 6:9:15.The fabricated indicator labels were attached inside the package headspace and observed an increase in CO_(2)(0.1±0.01 to 0.9±0.03%v/v)and a decrease in O_(2)(19.6±0.01 to 18.8±0.03%v/v)during storage(0-18 days).Color change of indicator labels was mainly caused by headspace concentrations that changed from dark to colorless for fresh and spoiled pomegranate arils.The PP-NMF used in MPPAs has high barrier properties,extending its shelf life in the refrigerator to over 15 days.The pomegranate arils stored for different periods of time showed significant changes in their physicochemical parameters.Over storage days,aerobic mesophilic bacteria(AMB)and yeast and mold count(YMC)increased to 4.90±0.20 and 4.50±0.20 log CFU g1.An indicator developed for monitoring pomegranate arils stored in the refrigerator can detect freshness efficiently.
