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ARID1B基因缺失促进NSCLC细胞的增殖、迁移和侵袭能力的研究
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作者 朱琳琳 张绪超 《中国肺癌杂志》 北大核心 2025年第3期165-175,共11页
背景与目的SWI/SNF染色质重塑复合物(switch/sucrose nonfermentable chromatin-remodeling complex)的异常与多种癌症密切相关,ARID1B(AT-rich interaction domain 1B)是SWI/SNF复合物的核心亚基之一。ARID1B基因突变或拷贝数缺失与DN... 背景与目的SWI/SNF染色质重塑复合物(switch/sucrose nonfermentable chromatin-remodeling complex)的异常与多种癌症密切相关,ARID1B(AT-rich interaction domain 1B)是SWI/SNF复合物的核心亚基之一。ARID1B基因突变或拷贝数缺失与DNA损伤反应受损、染色质可及性改变有关。然而,ARID1B缺失是否影响非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞的增殖、迁移和侵袭能力及其分子机制仍缺乏系统研究。本研究旨在揭示ARID1B基因缺失对NSCLC细胞恶性表型的调控作用及其分子机制。方法通过公共数据库分析ARID1B基因与肺癌患者预后之间的相关性以及其在肺癌组织中的表达水平。CRISPR/Cas9(clustered regularly interspaced short palindromic repeat)技术构建ARID1B基因稳定敲除(knockout,KO)的细胞株。采用平板克隆实验检测细胞增殖情况,Transwell细胞迁移、侵袭实验检测细胞迁移能力变化。RNA-Seq进行差异基因的表达、富集分析。利用蛋白印迹(Western blot,WB)验证ARID1B基因敲除效果,检测上皮间充质转化(epithelial-mesenchymal transition,EMT)标志物、促分裂素原活化蛋白激酶(mitogen-activated protein kinases,MAPK)信号通路相关蛋白变化。构建裸鼠成瘤模型,并比较对照和ARID1B缺失细胞的成瘤能力。结果低ARID1B表达与肺癌患者不良预后有显著关联,其总生存期较短。ARID1B在肺癌细胞中的表达水平较正常细胞显著降低。ARID1B缺失型细胞增殖、迁移和侵袭能力增强。动物实验中,ARID1B基因缺失组成瘤速度加快。RNA-Seq结果进行富集分析可见差异基因主要在MAPK、磷脂酰肌醇3-激酶/蛋白激酶B(phosphoinositide 3-kinase/protein kinase B,PI3K/Akt)等信号通路富集。WB证明ARID1B基因缺失细胞E-cadherin、N-cadherin、Vimentin表达发生变化,MAPK、p-MAPK表达增加。结论成功建立A549-ARID1B KO和PC9-ARID1B KO细胞株,ARID1B缺失细胞株在体外、体内生物学行为水平和转录组测序水平均提示具有高迁移、侵袭、增殖潜能。EMT标志物表达的变化、MAPK信号通路的激活提示ARID1B缺失型NSCLC可能的转移机制。 展开更多
关键词 arid1B 肺肿瘤 增殖 迁移 MAPK 转录组学分析
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ARID5B基因多态性与儿童急性淋巴细胞白血病风险及微小残留病的关系研究
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作者 马钰 高雅黛 +2 位作者 郭静 郑晓敏 张晓春 《中国实验血液学杂志》 北大核心 2025年第5期1269-1273,共5页
目的:探讨ARID5B基因单核苷酸多态性与宁夏地区回、汉族儿童急性淋巴细胞白血病(ALL)风险及微小残留病(MRD)的相关性。方法:采用病例对照研究,使用荧光共振能量转移技术,对54例ALL患儿及年龄、性别、民族相匹配的对照人群的ARID5B基因... 目的:探讨ARID5B基因单核苷酸多态性与宁夏地区回、汉族儿童急性淋巴细胞白血病(ALL)风险及微小残留病(MRD)的相关性。方法:采用病例对照研究,使用荧光共振能量转移技术,对54例ALL患儿及年龄、性别、民族相匹配的对照人群的ARID5B基因多态性位点进行检测,分析不同基因型ALL的易感性及其与MRD的相关性。结果:两组患儿rs10994982、rs7089424、rs10740055、rs7073837、rs4245595、rs7090445位点基因型及等位基因频率比较差异均无统计学意义(P>0.05)。在rs10821936位点上,ALL组T/T基因型和T等位基因频率均显著高于对照组(均P<0.05),ARID5B基因rs10821936的C/C基因型是ALL患儿MRD早期阳性的危险因素(P<0.05)。结论:ARID5B基因rs10821936与儿童ALL发生及MRD有一定相关性。 展开更多
关键词 儿童 急性淋巴细胞白血病 arid5B基因 单核苷酸多态性 微小残留病
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ARID1B基因变异所致Coffin-Siris综合征合并孤独症谱系障碍2例报道并文献复习
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作者 李梦月 李慧慧 +6 位作者 黄永 王甘雨 韩惠惠 孙爽 史晓依 王阳阳 王亚哲 《中国儿童保健杂志》 北大核心 2025年第1期112-116,共5页
Coffin-Siris 综合征(Coffin-Siris syndrome, CSS)是罕见的遗传性综合征,为常染色体显性遗传,其临床表现为不同程度的智力障碍、言语障碍、面部粗糙、生长障碍、发育迟缓伴第五指指甲或指骨缺如、胼胝体发育不全[1]。其他临床表型特征... Coffin-Siris 综合征(Coffin-Siris syndrome, CSS)是罕见的遗传性综合征,为常染色体显性遗传,其临床表现为不同程度的智力障碍、言语障碍、面部粗糙、生长障碍、发育迟缓伴第五指指甲或指骨缺如、胼胝体发育不全[1]。其他临床表型特征,如喂养困难、行为异常、听力障碍、癫痫发作及反复感染等症状。当前,已报道多个基因和CSS疾病相关,如SMARCA4、ARID1A、SMARCB1、ARID1B和SMARCE1等[2]。 展开更多
关键词 Coffin-Siris综合征 孤独症谱系障碍 arid1B基因 基因突变
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ARID1A与PIK3CA突变在卵巢子宫内膜异位症恶变中的研究进展 被引量:1
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作者 张云凤 张宛玥 +4 位作者 卢悦 王阳阳 井佳雨 牟婧祎 王悦(审校) 《国际妇产科学杂志》 2025年第1期19-22,共4页
子宫内膜异位症是一种常见的妇科疾病,育龄期女性的发病率为6%~10%。虽然子宫内膜异位症在形态学上呈良性表现,但易发生种植、侵袭及远处转移。卵巢子宫内膜异位症是最常见的子宫内膜异位症之一,治疗后易复发,严重威胁女性生殖健康。近... 子宫内膜异位症是一种常见的妇科疾病,育龄期女性的发病率为6%~10%。虽然子宫内膜异位症在形态学上呈良性表现,但易发生种植、侵袭及远处转移。卵巢子宫内膜异位症是最常见的子宫内膜异位症之一,治疗后易复发,严重威胁女性生殖健康。近年来大量研究发现,卵巢子宫内膜异位症具有恶变为卵巢癌的潜力。卵巢子宫内膜异位症恶变过程中最常见的是AT丰富结合域1A(AT-rich interaction domain 1A,ARID1A)与磷脂酰肌醇3激酶催化亚单位α(phosphatidylinositol 3-kinase catalytic subunit alpha,PIK3CA)基因突变,且发生在恶变早期。综述了ARID1A与PIK3CA突变在卵巢子宫内膜异位症恶变中的研究现状。 展开更多
关键词 子宫内膜异位症 卵巢肿瘤 基因 突变 arid1A PIK3CA
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ARID1A通过AKT信号通路调控肺腺癌细胞增殖能力
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作者 刘智荣 赵淑芳 王龙飞 《沈阳医学院学报》 2025年第3期244-249,共6页
目的:探讨肿瘤抑癌基因ARID1A(AT-rich interaction domain 1A)对肺腺癌细胞增殖的调控作用,及其与蛋白激酶B(protein kinase B,AKT)信号通路的分子机制的关联。方法:采用慢病毒转染技术构建ARID1A稳定过表达的A549/H1299细胞模型,通过R... 目的:探讨肿瘤抑癌基因ARID1A(AT-rich interaction domain 1A)对肺腺癌细胞增殖的调控作用,及其与蛋白激酶B(protein kinase B,AKT)信号通路的分子机制的关联。方法:采用慢病毒转染技术构建ARID1A稳定过表达的A549/H1299细胞模型,通过RT-PCR和Western blot验证转染效率;利用CCK-8检测细胞活力、EdU染色法评估细胞增殖率;划痕实验分析细胞迁移能力;活/死双染(Calcein/PI染色)与台盼蓝染色检测细胞死亡;Western blot检测AKT磷酸化水平;通过AKT抑制剂MK-2206(1μmol/L)处理过表达细胞,验证AKT通路在增殖调控中的作用。结果:ARID1A过表达显著抑制A549/H1299细胞增殖及迁移能力,并上调p-AKT水平;MK-2206处理后,过表达ARID1A组细胞增殖率被明显抑制;细胞死亡实验显示,过表达ARID1A对A549/H1299细胞死亡率无显著影响。结论:ARID1A通过激活AKT信号通路特异性抑制肺腺癌细胞增殖及迁移,提示靶向AKT通路可能为ARID1A缺失型肺腺癌患者的精准治疗提供潜在靶点。 展开更多
关键词 arid1A 肺腺癌 AKT 细胞增殖
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Projection of extreme climate change in the Asian arid region and the Tibetan Plateau in the early and middle 21st century based on NEX-GDDP-CMIP6 被引量:1
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作者 Yankun Sun Rui Zhu Tao Wang 《Atmospheric and Oceanic Science Letters》 2025年第3期53-58,共6页
In the context of global warming,it is anticipated that both the intensity and the frequency of future global extreme high precipitation(EHP)and extreme high temperature(EHT)events will increase.To evaluate the future... In the context of global warming,it is anticipated that both the intensity and the frequency of future global extreme high precipitation(EHP)and extreme high temperature(EHT)events will increase.To evaluate the future extreme climate changes in the Asian arid region and Tibetan Plateau,this study applied the NASA Earth Exchange Global Daily Downscaled Projections(NEX-GDDP-CMIP6)to assess the changes in EHP(Rx5d and R95pTOT)and EHT(TX90p and TXx)under different emission scenarios in the 21st century.Findings suggest that both the frequency and the intensity of the extreme indices will increase,exhibiting accelerated growth under higher emission scenarios,particularly under the SSP5-8.5 emission scenario.It is suggested that the growth of EHT and EHP in the early subperiod of the 21st century(2026-2045)will be relatively moderate,with small differences between different emission scenarios.However,by the middle subperiod of the 21st century(2041-2060),the differences between different emission scenarios will become larger than the 2035s and the growth will become more intense.In western central Asia,TX90p,TXx,Rx5d,and R95pTOT increase by 9.7%-14.2%(13.3%-24.7%),1.3℃-1.7℃(1.6℃-2.7℃),6.5%-8.9%(8.2%-8.8%),and 18.1%-27.0%(25.6%-30.0%)by the early(middle)subperiod;in eastern central Asia,TX90p,TXx,Rx5d,and R95pTOT increase 8.1%-12.0%(11.3%-21.1%),1.4℃-1.8℃(1.9℃-2.9℃),7.4%-9.7%(10.4%-13.8%),and 20.2%-29.3%(32.0%-40.8%)by the early(middle)subperiod;and over the Tibetan Plateau,TX90p,TXx,Rx5d,and R95pTOT increase 12.5%-17.4%(17.0%-31.0%),1.2℃-1.5℃(1.6℃-2.5℃),7.2%-10.0%(9.9%-15.0%),and 26.6%-33.1%(36.1%-55.3%)by the early(middle)subperiod. 展开更多
关键词 Extreme high precipitation Extreme high temperature Asian arid region Tibetan plateau NEX-GDDP-CMIP6
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Variations of soil moisture and its influencing factors in arid and semi-arid areas,China 被引量:1
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作者 NIU Jiqiang LIU Zijian +5 位作者 CHEN Feiyan LIU Gangjun ZHOU Junli ZHOU Peng LI Hongrui LI Mengyang 《Journal of Arid Land》 2025年第5期624-643,共20页
Soil moisture(SM)is a critical variable in terrestrial ecosystems,especially in arid and semi-arid areas where water sources are limited.Despite its importance,understanding the spatiotemporal variations and influenci... Soil moisture(SM)is a critical variable in terrestrial ecosystems,especially in arid and semi-arid areas where water sources are limited.Despite its importance,understanding the spatiotemporal variations and influencing factors of SM in these areas remains insufficient.This study investigated the spatiotemporal variations and influencing factors of SM in arid and semi-arid areas of China by utilizing the extended triple collation(ETC),Mann-Kendall test,Theil-Sen estimator,ridge regression analysis,and other relevant methods.The following findings were obtained:(1)at the pixel scale,the long-term monthly SM data from the European Space Agency Climate Change Initiative(ESA CCI)exhibited the highest correlation coefficient of 0.794 and the lowest root mean square error(RMSE)of 0.014 m^(3)/m^(3);(2)from 2000 to 2022,the study area experienced significant increase in annual average SM,with a rate of 0.408×10^(-3)m^(3)/(m^(3)•a).Moreover,higher altitudes showed a notable upward trend,with SM increasing rates at 0.210×10^(-3)m^(3)/(m^(3)•a)between 1000 and 2000 m,0.530×10^(-3)m^(3)/(m^(3)•a)between 2000 and 4000 m,and 0.760×10^(-3)m^(3)/(m^(3)•a)at altitudes above 4000 m;(3)land surface temperature(LST),root zone soil moisture(RSM)(10-40 cm depth),and normalized difference vegetation index(NDVI)were identified as the primary factors influencing annual average SM,which accounted for 34.37%,24.16%,and 22.64%relative contributions,respectively;and(4)absolute contribution of LST was more significant in subareas at higher altitudes,with average absolute contributions of 0.800×10^(-3)m^(3)/(m^(3)•a)between 2000 and 4000 m and 0.500×10^(-2) m^(3)/(m^(3)•a)above 4000 m.This study reveals the spatiotemporal variations and main influencing factors of SM in Chinese arid and semi-arid areas,highlighting the more pronounced absolute contribution of LST to SM in high-altitude areas,providing valuable insights for ecological research and water resource management in these areas. 展开更多
关键词 soil moisture arid and semi-arid areas remote sensing extended triple collation ridge regression analysis
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Erratum to:Flooding(or breaching)of inter-connected proglacial lakes by cascading overflow in the arid region of Western Mongolia(Mt.Tsambagarav,Mongolian Altai)
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作者 Otgonbayar DEMBEREL Chinmay DASH +6 位作者 Battsetseg DUGERSUREN Munkhbat BAYARMAA Yeong Bae SEONG Elora CHAKRABORTY Batsuren DORJSUREN Atul SINGH Nemekhbayar GANHUYAG 《Journal of Mountain Science》 2025年第5期1888-1888,共1页
The author affiliation and the funding information in the Acknowledgement section of the online version of the original article was revised.One affiliation(the 8th affiliation)of the first author is added.The Acknowle... The author affiliation and the funding information in the Acknowledgement section of the online version of the original article was revised.One affiliation(the 8th affiliation)of the first author is added.The Acknowledgement section of the original article has been revised to:Acknowledgments:This research was funded by the National University of Mongolia under grant agreement P2023(grant number P2023-4578)and supported by the Chey Institute for Advanced Studies“International Scholarship Exchange Fellowship for the academic year of 2024-2025”,Republic of Korea,and the National University of Mongolia.We would like to acknowledge the National University of Mongolia and Soumik Das from the Center for the Study of Regional Development,Jawaharlal Nehru University,New Delhi-110067,for his valuable assistance in preparing the geological maps. 展开更多
关键词 Mongolia proglacial lakes Mongolian Altai arid region tsambagarav cascading overflow geological maps FLOOD
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The Impact of Intensified Aridization Caused by Moisture Deficit on the Productivity of Grain Crops in Northern Kazakhstan
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作者 Aisulu Amirkhanovna Kusainova Galina Nikolaevna Chistyakova Gaukhar Makhanovna Zhangozhina 《Research in Ecology》 2025年第3期199-211,共13页
The article examines the impact of increased aridization of the territory due to an increase in air temperature,reduced precipitation,and the formation of moisture deficiency on grain yields in Northern Kazakhstan.The... The article examines the impact of increased aridization of the territory due to an increase in air temperature,reduced precipitation,and the formation of moisture deficiency on grain yields in Northern Kazakhstan.The most important result of the work is the revealed inverse relationship between grain yields and the temperature of the growing season:low-yielding years are associated with high temperatures and droughts,and high-yielding years are associated with lower temperatures and an optimal ratio of heat and moisture.The novelty of this study is the use of the method of hydrological and climatic calculations in identifying the nature of temperature variability and precipitation in the territory of Northern Kazakhstan for the modern period(1991–2020)compared with the base period(1961–1990).At all the studied meteorological stations,there is a tendency for the average annual temperature and the temperature of the growing season to increase:in the forest-steppe zone with an average warming intensity of 0.3–0.33℃ per decade;in the steppe zone by 0.2–0.43℃ per decade;and in the growing season by 0.2–0.7℃ per decade.The air temperature in the steppe zone is rising more intensively than in the forest-steppe zone,and precipitation in the forest-steppe zone has changed more than in the steppe zone.An increase in the average annual air temperature during the growing season(May–August),combined with a shortage of atmospheric moisture or a constant amount of it,led to an increase in the degree of aridization of the territory,an increase in the frequency of droughts in the steppe zone of Northern Kazakhstan. 展开更多
关键词 aridization Air Temperature Precipitation Moisture Deficiencies Crop Yield Northern Kazakhstan
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Analysis of Processes and Drivers of River Evolution in Arid Zones Under the Influence of Natural and Different Levels of Human Activities:A Case Study of the Shule River
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作者 GAO Mingjun LI Yu +4 位作者 SHANG Hao ZHANG Zhansen LIU Shiyu DUAN Junjie XUE Yaxin 《宁夏大学学报(自然科学版中英文)》 2025年第3期302-316,共15页
Based on regional paleoclimate sequences,records of human activities,paleoclimate simulations,and detailed environmental historical records,we discuss the impacts of Holocene climate change and human activities on the... Based on regional paleoclimate sequences,records of human activities,paleoclimate simulations,and detailed environmental historical records,we discuss the impacts of Holocene climate change and human activities on the evolution of the Shule River in the western Qilian Mountains,China.The results indicate that during the early to mid-Holocene,the river evolution of the Shule River alluvial fan was closely related to regional climate fluctuations.In the late Holocene,flood agriculture began to emerge along the Shule River.During the historical period,population growth and the expansion of arable land led to increased river water usage,resulting in decreased access to the expected distribution of water resources in other regions,which in turn has caused imbalances in the regional hydrological ecosystem. 展开更多
关键词 river evolution arid region human activity ancient climate driving factors Shule River
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Vegetation Response to Soil and Morphological Properties in an Arid Region Alluvial Fan Landscape,Pre-Andes of San Juan,Argentina
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作者 Daniel Germán Flores Raúl Emmanuel Ocaña +3 位作者 Edgardo Melián Andrés Ortega María Yanina Esper Angillieri María Alejandra Pittaluga 《Journal of Earth Science》 2025年第2期408-427,共20页
The Monte Desert is characterized by a great diversity of landforms created with fluvial,alluvial which the vegetation patterns are related to.The present work has the following objectives:(1) determine whether topogr... The Monte Desert is characterized by a great diversity of landforms created with fluvial,alluvial which the vegetation patterns are related to.The present work has the following objectives:(1) determine whether topographical attributes,surface characteristics,soil properties and vegetation patterns vary between alluvial landforms,and(2) define whether morphometric,soil and surface properties influence vegetation patterns along alluvial landscape.Morphometric data were obtained by processing a 5 m digital elevation model.The coverage of rock fragments,fine sediments and mulch was quantified.Observations and descriptions of the soil profiles were restricted to the uppermost 50 cm.Vegetation properties were calculated using a Point Quadrat Method.The relationship between variables was evaluated through multivariate statistical analysis.The main results show the presence of 45 plant species distributed in 19 families,where shrubs are dominant.The wind effect,topographic wetness and dissection of the landscape are limiting factors of diversity.The coverage of superficial rock fragments influence vegetation coverage through the distribution and availability of rainwater.Furthermore,the different soil textures reveal that the silt content favors an increase in vegetation coverage.The presence of V horizon could condition the installation and development of vegetation in the early stages of growth. 展开更多
关键词 alluvial geomorphology soil texture vegetation patterns arid landscapes MORPHOMETRICS environmental geology
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Dust storm variation in arid West Asia regulated by subtropical high during the Holocene
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作者 CHEN Shengqian CHEN Zhitong +6 位作者 MA Shuai CHEN Jianhui ZHOU Aifeng WU Duo KHORMALI Farhad HOU Juzhi CHEN Fahu 《Journal of Geographical Sciences》 2025年第8期1743-1760,共18页
Arid West Asia(AWA)is a critical hub of the Silk Road and one of the primary dust source regions in the Northern Hemisphere.Dust storms in AWA emitting substantial dust particles into the atmosphere,significantly infl... Arid West Asia(AWA)is a critical hub of the Silk Road and one of the primary dust source regions in the Northern Hemisphere.Dust storms in AWA emitting substantial dust particles into the atmosphere,significantly influencing air quality,climate change and marine productivity.However,the variability of dust storm activity in this region during the Holocene,particularly its links to vegetation and hydroclimatic changes,remains debated,hindering our understanding of the interconnected dynamics between climate change and surface environments.This study reconstructs dust storm variations in AWA over the past 9000 years using geochemical analyses(trace elements,Sr-Nd isotopes)from a well-dated,high-resolution sediment core from the Almalou Peatland,located on the western Iranian Plateau.Our results reveal a decline in dust storm frequency from the early to mid-Holocene,a minimum occurrence during the mid-Holocene,and a significant increase in the late Holocene.Provenance analysis indicates that the primary dust sources were the arid regions of Mesopotamia,located upwind of the study area.A comparison with proxy records and paleoclimate models suggests an inverse relationship between dust storm activity and regional hydroclimatic and vegetation changes,along with a positive correlation with wind speeds.The concentration of dust storms during the wetter month of May highlights wind speed as a more critical driving factor.Moreover,given the dominant influence of the subtropical high on hydroclimatic conditions and wind speeds in AWA,we propose that this system is the key regulator of regional dust storm dynamics.Our findings provide new insights into the drivers of dust storm activity in AWA and hold implications for developing targeted dust storm management strategies. 展开更多
关键词 arid West Asia HOLOCENE dust storms subtropical high wind regimes
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Altitudinal patterns of plant diversity, cover, and life forms in a warm arid mountain: Insights from Sierra de Velasco, Argentina
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作者 Sofia V.LIZARRAGA Omar VARELA Julieta CARILLA 《Journal of Mountain Science》 2025年第3期873-887,共15页
Mountains are important reservoirs of biodiversity and endemism on a global scale, but little is known about the altitudinal configuration of this diversity and its driving factors in arid mountains. We explored varia... Mountains are important reservoirs of biodiversity and endemism on a global scale, but little is known about the altitudinal configuration of this diversity and its driving factors in arid mountains. We explored variations in composition, diversity, cover,and life forms of vascular plants along a complete altitudinal gradient(1300-4000 m a.s.l) in Sierra de Velasco, an arid mountain in northwestern Argentina.We evaluated the influence of environmental variables on plant diversity and cover. Field sampling was conducted in the northern sector of the Sa. de Velasco,on the western slope in eight 50 m wide altitudinal bands at 400 m elevation intervals. We used rectangular plots(20 × 4 m;n:10/altitudinal band) to register the vascular plants of all the growth forms, and linear transects(20 m long.;n:30/altitudinal band) to quantify the vegetation cover using the point intercept method. Diversity was calculated using hill numbers.Data analysis included non-metric multidimensional scaling(NMDS), indicator species analysis,generalized linear models(GLMs), and variance partitioning analysis. A total of 232 species from 51families and 158 genera were registered. Species composition showed greater similarity at intermediate elevations. Plant diversity and cover exhibited a unimodal distribution, peaking at intermediate elevations(2100-2500 m). In contrast, life forms' distribution showed divergent patterns. Therophytes and succulents predominated at low altitudes,phanerophytes and hemicryptophytes at medium altitudes, and chamaephytes and geophytes at high altitudes. The altitudinal patterns of plant diversity and cover were primarily driven by climatic factors.Conservation efforts in the Sierra de Velasco should focus on the middle and upper zones due to their high biodiversity and vulnerability to climate change. 展开更多
关键词 Elevation gradient Plant diversity Life forms arid mountains Diversity pattern Climatic drivers
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ARID1A对肝癌患者预后和中性粒细胞浸润影响的分析及验证
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作者 王梦成 孟涛 +1 位作者 蔚应飞 童钟 《中国现代医药杂志》 2025年第4期21-29,共9页
目的 在肝癌组织中寻找与中性粒细胞浸润相关的分子,并探究其在肿瘤治疗和评估中的作用。方法 从既往文献中筛选出中性粒细胞的标志基因。基于这些基因,使用R软件包“ConsensusClusterPlus”将肝癌患者划分为不同的亚型。通过单样本基... 目的 在肝癌组织中寻找与中性粒细胞浸润相关的分子,并探究其在肿瘤治疗和评估中的作用。方法 从既往文献中筛选出中性粒细胞的标志基因。基于这些基因,使用R软件包“ConsensusClusterPlus”将肝癌患者划分为不同的亚型。通过单样本基因集富集分析(ssGSEA)和基因集富集分析(GSEA)探究不同亚型间的生物学差异。使用“Limma”包鉴定不同亚型间的差异表达基因。利用GPSAdb网站鉴定与亚型具有相似特征的分子靶点,并通过TIMER及MCPcounter算法分析其对肝癌组织中中性粒细胞浸润程度的影响。通过免疫组织化学染色法检测癌症组织和癌旁组织中AT丰富结合域1A (AT-rich interaction domain 1A,ARID1A)的表达水平及中性粒细胞浸润水平,并分析其与患者临床病理参数的关系。最后,结合TCGA和GEO数据库探究该分子与肝癌患者预后和索拉非尼治疗敏感性的关系。结果 基于10个中性粒细胞标志基因,将肝癌患者分为两个亚型。两个亚型的肝癌患者预后存在显著差异。中性粒细胞标志基因主要与白细胞迁移及细胞免疫等细胞功能相关。GPSAdb网站识别出ARID1A与肝癌的中性粒细胞亚型具有相似特征。免疫组化实验结果表明,与癌旁组织相比,肝癌细胞中ARID1A的表达水平显著升高,且ARID1A高表达的组织中,中性粒细胞浸润水平也显著升高。TIMER及MCPcounter算法分析表明,ARID1A促进肝癌中中性粒细胞的浸润,并预测肝癌患者的不良预后和对索拉非尼治疗的耐药性。结论 ARID1A是预测肝癌患者预后的一个生物标志物,并促进肝癌中中性粒细胞浸润和索拉非尼耐药。 展开更多
关键词 arid1A 肝癌 中性粒细胞 免疫浸润
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Adaptive conservation of natural resources determines the sustainable livelihood strategies of farmers in the semi-arid Loess Plateau region of China
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作者 MA Shengli XU Mingxiang 《Regional Sustainability》 2025年第4期26-40,共15页
Livelihood improvement and sustainable natural resource utilization are among the United Nations Sustainable Development Goals;however,most farm households either randomly select or just imitate livelihood strategies ... Livelihood improvement and sustainable natural resource utilization are among the United Nations Sustainable Development Goals;however,most farm households either randomly select or just imitate livelihood strategies being practiced in other areas.As the livelihoods of farm households are vulnerable to several challenges in ecologically fragile areas,identifying the livelihood strategies of farm households in arid and semi-arid areas will help for promoting both livelihood security and environmental conservation.Hence,in this study,we constructed a sustainable evaluation index system for the livelihood of farmers in the Loess Plateau region of China by conducting field research and interviews.We employed the composite index method to measure the livelihood capital and the livelihood strategies chosen by farmers,and then analyzed the resource endowment of farmers under different livelihood strategies using logistic regression and structural equation modeling.The results revealed that under the combined influences of livelihood capital and government policy,farm households in the Loess Plateau region selected agro-dependent,agro-pastoral,agro-industrial,non-agricultural livelihood strategies.The key factors influencing the selection of these household livelihood strategies included the household labor capacity,the farmland owned per household,the number of livestock,and the grassland forage-supply ratio.The grassland forage-supply ratio was a crucial factor influencing the choice between agricultural and non-agricultural livelihood strategies.In this context,the grassland forage-supply ratio increased with the stocking rate.However,once the stocking rate exceeded 56.5 sheep/hm^(2),the grassland forage-supply ratio no longer increased rapidly under the agriculture-based livelihood strategy.The strategy of ecological resource conservation and moderate utilization based on the quality and quantity of natural resources ensures win-win benefits for the environment and human well-being in arid and semi-arid areas.Moderate grazing therefore has the potential to improve farmers’livelihoods without causing grassland degradation.These results contribute to the synergistic co-adaptation of livelihood improvement and ecological conservation in arid and semi-arid areas. 展开更多
关键词 Livelihood strategies Livelihood capital Farm household Grassland forage-supply ratio Structural equation modeling(SEM) arid and semi-arid areas Loess Plateau
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miR-340-5p靶向调控ARID1A对甲状腺癌活性、迁移和侵袭的影响 被引量:1
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作者 段飞 陈羿 +1 位作者 查官金 段训凰 《中国老年学杂志》 CAS 北大核心 2024年第6期1480-1485,共6页
目的 探讨miR-340-5p靶向调控富含AT的相互作用结构域(ARID)1A对甲状腺癌活性、迁移和侵袭的影响。方法 通过实时荧光定-聚合酶链反应(RT-qPCR)和Western印迹检测miR-340-5p和ARID1A在甲状腺癌细胞中表达水平。通过噻唑蓝(MTT)和Transw... 目的 探讨miR-340-5p靶向调控富含AT的相互作用结构域(ARID)1A对甲状腺癌活性、迁移和侵袭的影响。方法 通过实时荧光定-聚合酶链反应(RT-qPCR)和Western印迹检测miR-340-5p和ARID1A在甲状腺癌细胞中表达水平。通过噻唑蓝(MTT)和Transwell实验检测转染miR-340-5p抑制物后癌细胞活性、迁移和侵袭变化。双荧光素酶报告基因检测miR-340-5p和ARID1A的靶向结合关系。最后通过同时敲低miR-340-5p和ARID1A的回复实验验证miR-340-5p调控ARID1A影响癌症发展。结果 miR-340-5p在甲状腺癌细胞中明显高表达,ARID1A表达明显较低(P<0.05)。通过抑制BCPAP细胞中miR-340-5p表达明显减少了癌细胞活性、迁移和侵袭水平。miR-340-5p和ARID1A具有直接靶向结合关系。敲降ARID1A能够明显逆转miR-340-5p抑制物对BCPAP细胞活性、迁移和侵袭的抑制作用。结论 miR-340-5p是甲状腺癌中的促癌基因,ARID1A为miR-340-5p的靶标下游,miR-340-5p通过靶向调控ARID1A促进甲状腺癌细胞活性、迁移和侵袭。 展开更多
关键词 甲状腺癌 miR-340-5p 富含AT的相互作用结构域(arid)1A 细胞活性 转移
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结直肠癌组织中ARID1A基因突变及DEPDC1表达与临床病理特征及预后的相关性 被引量:3
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作者 王准证 陈聪 施稳稳 《海南医学》 CAS 2024年第18期2590-2594,共5页
目的探讨结直肠癌中ARID1A基因突变及DEPDC1表达与患者临床病理特征及预后的相关性。方法回顾性分析2017年5月至2019年5月漯河市中心医院收治的152例结直肠癌患者的临床资料,所有患者均采用测序分析法和BCA法分析癌组织样本中ARID1A基... 目的探讨结直肠癌中ARID1A基因突变及DEPDC1表达与患者临床病理特征及预后的相关性。方法回顾性分析2017年5月至2019年5月漯河市中心医院收治的152例结直肠癌患者的临床资料,所有患者均采用测序分析法和BCA法分析癌组织样本中ARID1A基因的突变情况和DEPDC1的蛋白表达水平,依据ARID1A基因的突变情况和DEPDC1的蛋白表达水平分为ARID1A基因突变组、ARID1A基因未突变组和DEPDC1高表达组、DEPDC1低表达组,并评估ARID1A基因突变、DEPDC1表达与肿瘤的淋巴结转移、临床分期、大小和病理分化等临床病理特征的关系。使用Kaplan-Meier生存曲线分析ARID1A基因突变及DEPDC1高表达对患者5年生存率的影响。结果ARID1A基因突变组患者的低分化占比、Ⅲ~Ⅳ期占比、脉管侵犯占比均为63.64%(21/33)、54.55%(18/33)和60.61(20/33),明显高于ARID1A基因未突变组的29.41%(35/119)、24.37%(29/119)和31.93%(38/119),差异均有统计学意义(P<0.05);DEPDC1高表达组患者的Ⅲ~Ⅳ期占比、淋巴转移占比分别为46.51%(20/43)、53.49%(23/43),明显高于DEPDC1低表达组的24.77%(27/109)、24.77%(27/109),差异均有统计学意义(P<0.05);Kaplan-Meier生存分析结果显示,ARID1A基因未突变和DEPDC1低表达的患者5年生存率分别为70.59%和84.40%,明显高于ARID1A基因突变患者的39.39%和DEPDC1高表达患者的48.84%,差异均有统计学意义(P<0.05)。结论ARID1A基因突变及DEPDC1高表达可能在结直肠癌的发展和预后中发挥作用,为结直肠癌的分子分类和个体化治疗提供潜在的靶点。 展开更多
关键词 arid1A DEPDC1 结直肠癌 临床病理特征 预后
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Estimating the Drought-Induced Yield Loss for Winter Wheat in a Semi-Arid Region of the Southern United States Using a Drought Index
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作者 Prem Woli Qingwu Xue +2 位作者 Gerald R. Smith Charles R. Long Francis M. Rouquette Jr. 《Agricultural Sciences》 2024年第8期812-829,共18页
The economy of most rural locations in the semi-arid region of Llano Estacado in the southern United States is predominantly based on agriculture, primarily beef and wheat (Triticum aestivum L.) production. This regio... The economy of most rural locations in the semi-arid region of Llano Estacado in the southern United States is predominantly based on agriculture, primarily beef and wheat (Triticum aestivum L.) production. This region is prone to drought and is projected to experience a drier climate. Droughts that coincide with the critical phenological phases of a crop can be remarkably costly. Although drought cannot be prevented, its losses can be minimized through mitigation measures if it is predicted in advance. Predicting yield loss from an imminent drought is an important need of stakeholders. One way to fulfill this need is using an agricultural drought index, such as the Agricultural Reference Index for Drought (ARID). Being plant physiology-based, ARID can represent drought-yield relationships accurately. This study developed an ARID-based yield model for predicting the drought-induced yield loss for winter wheat in this region by accounting for its phenological phase-specific sensitivity to water stress. The reasonable values of the drought sensitivity coefficients of the yield model indicated that it could reflect the phenomenon of water stress decreasing the winter wheat yields in this region reasonably. The values of the various metrics used to evaluate the model, including Willmott Index (0.86), Nash-Sutcliffe Index (0.61), and percentage error (26), indicated that the yield model performed fairly well at predicting the drought-induced yield loss for winter wheat. The yield model may be useful for predicting the drought-induced yield loss for winter wheat in the study region and scheduling irrigation allocation based on phenological phase-specific drought sensitivity. 展开更多
关键词 arid DROUGHT Drought index Growth-stage Model Phenological-Phase Prediction SEMI-arid Wheat Yield
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