Background:Much still remains unknown about the pathogenesis of brain arteriovenous malformations(AVMs).Previous studies have revealed the abnormal expression of various angiogenesis-related genes in AVMs.To further u...Background:Much still remains unknown about the pathogenesis of brain arteriovenous malformations(AVMs).Previous studies have revealed the abnormal expression of various angiogenesis-related genes in AVMs.To further understand this disease,we sought to identify genes differently expressed in AVMs by means of the gene microarray technique.Methods:Nine AVMs specimen and nine samples of normal vessels are collected.Total RNA isolated from these specimen is hybridized with Oligonucleotide array and gene analysis was conducted.Analyzing data with the help of significance analysis of microarrays(SAM)and a free web-based molecular annotation system 3.0(MAS 3.0).Results:The SAM method identify 37 gene significantly up-regulated and 10 genes down-regulated in AVMs.Conclusions:Among those genes,VACN,SPARK and ARHGAP18 seem to play a facilitating role during the genesis of AVMs.Multiple pathways,as MAPK pathway,may also be involved.展开更多
基金study received financial support from the Beijing Nova program of science and technology.The funding organizations had no role in development of the study or the drafting of the manuscript for publication.
文摘Background:Much still remains unknown about the pathogenesis of brain arteriovenous malformations(AVMs).Previous studies have revealed the abnormal expression of various angiogenesis-related genes in AVMs.To further understand this disease,we sought to identify genes differently expressed in AVMs by means of the gene microarray technique.Methods:Nine AVMs specimen and nine samples of normal vessels are collected.Total RNA isolated from these specimen is hybridized with Oligonucleotide array and gene analysis was conducted.Analyzing data with the help of significance analysis of microarrays(SAM)and a free web-based molecular annotation system 3.0(MAS 3.0).Results:The SAM method identify 37 gene significantly up-regulated and 10 genes down-regulated in AVMs.Conclusions:Among those genes,VACN,SPARK and ARHGAP18 seem to play a facilitating role during the genesis of AVMs.Multiple pathways,as MAPK pathway,may also be involved.
