Antibiotic resistance genes (ARGs) have attracted more and more attention due to their potential exposure hazards. The Danjiangkou Reservoir (DJKR) is the source of water for the Middle Route Project under the South-t...Antibiotic resistance genes (ARGs) have attracted more and more attention due to their potential exposure hazards. The Danjiangkou Reservoir (DJKR) is the source of water for the Middle Route Project under the South-to-North Water Transfer Scheme in China. To clarify the distribution of ARGs and their influencing factors in DJKR (including Danjiang Reservoir (DR) and Hanjiang River Reservoir (HR)), we used metagenomic analysis to investigate the ARGs. The results showed that the most abundant bacteria of both parts were Proteobacteteria. Antibiotic efflux (58.2 %) and alteration of antibiotic targets (69.4 %) were the main mechanisms in DR and HR. The composition of ARG species was similar in the two parts, but the number of ARG isoforms in HR was significantly higher than that in DR. ARG Intl1 was detected in both DR and HR. Network analysis showed a significant correlation between mobile genetic elements (MGEs) and ARGs. Heavy metals also showed a significant correlation with ARGs. Interestingly, the relationship between heavy metals and ARGs were more significant than that between antibiotics and ARGs.展开更多
The coexistence of emerging containments,such as antibiotic resistant bacteria(ARB),antibiotic-resistant genes(ARGs)and antibiotics,potentially influence elimination efficiencies in UV light-emitting diode(UV-LED)alon...The coexistence of emerging containments,such as antibiotic resistant bacteria(ARB),antibiotic-resistant genes(ARGs)and antibiotics,potentially influence elimination efficiencies in UV light-emitting diode(UV-LED)alone and UV-LED/H_(2)O_(2) system as their complex interactions.Tetracycline(TC)degradation efficiency(kF)correlated closely with its UV molar absorbance(R^(2)=0.831)in UV-LED alone system and with·OH yield(R^(2)=0.999)in UV-LED/H_(2)O_(2) system across studied wavelengths(265,280 and 310 nm).The kF values for intracellular DNA(i-ARGs)also exhibited a high correlation with UV-LED wavelengths in both systems(R^(2)=0.997-0.999).The coexistence of TC and ARB/ARGs resulted in a mutual inhibition of their degradation efficiencies due to competition for photons and·OH,along with the consequent reduction in intracellular ROS within ARB,with their degradation efficiencies exhibiting marked dependence on wavelength in both systems.Notably,the UV-LED/H_(2)O_(2) system at 265 nm effectively achieved the simultaneous removal of TC,ARB and ARGs with minimal energy consumption,and successfully fragmented ARGs.The degradation pathway of TC was analyzed,and the biotoxicity of its degradation intermediates demonstrated the environmental friendliness and safety of UV-LED/H_(2)O_(2) technology.This study elucidated the competitive interactions between antibiotics and ARB/ARGs within UV-LED/H_(2)O_(2) system,providing a promising approach for their simultaneous removal while ensuring energy efficiency.展开更多
基金supported by the National Natural Science Foundation of China(41807409)the foundation for Innovative Research Team of Hubei Provincial Department of Education(JYT2021024)Advantages Discipline Group(Public health)Project in Higher Education of Hubei Province(2021-2025).
文摘Antibiotic resistance genes (ARGs) have attracted more and more attention due to their potential exposure hazards. The Danjiangkou Reservoir (DJKR) is the source of water for the Middle Route Project under the South-to-North Water Transfer Scheme in China. To clarify the distribution of ARGs and their influencing factors in DJKR (including Danjiang Reservoir (DR) and Hanjiang River Reservoir (HR)), we used metagenomic analysis to investigate the ARGs. The results showed that the most abundant bacteria of both parts were Proteobacteteria. Antibiotic efflux (58.2 %) and alteration of antibiotic targets (69.4 %) were the main mechanisms in DR and HR. The composition of ARG species was similar in the two parts, but the number of ARG isoforms in HR was significantly higher than that in DR. ARG Intl1 was detected in both DR and HR. Network analysis showed a significant correlation between mobile genetic elements (MGEs) and ARGs. Heavy metals also showed a significant correlation with ARGs. Interestingly, the relationship between heavy metals and ARGs were more significant than that between antibiotics and ARGs.
基金supported by Major Scientific and Technological Innovation Project of Shandong Province(No.2020CXGC011204)Qingdao Natural Science Foundation(No.23-2-1-234-zyyd-jch).
文摘The coexistence of emerging containments,such as antibiotic resistant bacteria(ARB),antibiotic-resistant genes(ARGs)and antibiotics,potentially influence elimination efficiencies in UV light-emitting diode(UV-LED)alone and UV-LED/H_(2)O_(2) system as their complex interactions.Tetracycline(TC)degradation efficiency(kF)correlated closely with its UV molar absorbance(R^(2)=0.831)in UV-LED alone system and with·OH yield(R^(2)=0.999)in UV-LED/H_(2)O_(2) system across studied wavelengths(265,280 and 310 nm).The kF values for intracellular DNA(i-ARGs)also exhibited a high correlation with UV-LED wavelengths in both systems(R^(2)=0.997-0.999).The coexistence of TC and ARB/ARGs resulted in a mutual inhibition of their degradation efficiencies due to competition for photons and·OH,along with the consequent reduction in intracellular ROS within ARB,with their degradation efficiencies exhibiting marked dependence on wavelength in both systems.Notably,the UV-LED/H_(2)O_(2) system at 265 nm effectively achieved the simultaneous removal of TC,ARB and ARGs with minimal energy consumption,and successfully fragmented ARGs.The degradation pathway of TC was analyzed,and the biotoxicity of its degradation intermediates demonstrated the environmental friendliness and safety of UV-LED/H_(2)O_(2) technology.This study elucidated the competitive interactions between antibiotics and ARB/ARGs within UV-LED/H_(2)O_(2) system,providing a promising approach for their simultaneous removal while ensuring energy efficiency.
