目的:探讨三结构域蛋白家族24(tripartite motif-containing protein 24,TRIM24)、c-myc及ARF蛋白在食管鳞状细胞癌组织中的表达及其意义。方法:选取2018年01月至2022年12月期间承德市中心医院接受手术治疗的102例食管鳞癌患者临床病理...目的:探讨三结构域蛋白家族24(tripartite motif-containing protein 24,TRIM24)、c-myc及ARF蛋白在食管鳞状细胞癌组织中的表达及其意义。方法:选取2018年01月至2022年12月期间承德市中心医院接受手术治疗的102例食管鳞癌患者临床病理资料作为研究对象(实验组),同时选取癌旁正常食管黏膜组织54例(对照组),采用免疫组化(Envision法)对TRIM24、c-myc及ARF蛋白进行检测,观察三者在鳞癌组织及癌旁正常食管黏膜组织中的表达情况,并分析三者的表达与临床病理特征的关系,同时研究三者于食管鳞癌组织内表达的相关性。结果:TRIM24、c-myc及ARF在鳞癌组织及癌旁正常食管黏膜组织中阳性表达率分别为72.62%vs 7.45%、74.36%vs 13.85%、26.56%vs 83.18%,三种蛋白在鳞癌组织及正常食管黏膜组织中的表达差异具有统计学意义(P均<0.05);经分析,食管鳞癌组织中的TRIM24、c-myc及ARF蛋白表达与癌的分化程度、淋巴结转移、临床分期具有显著相关性(P均<0.05);经Spearman相关性分析,食管鳞癌组织中TRIM24与c-myc蛋白表达呈正相关(r=0.587,P<0.05),TRIM24与ARF蛋白表达呈负相关(r=-0.601,P<0.05),c-myc与ARF蛋白表达呈负相关(r=-0.465,P<0.05)。结论:TRIM24、c-myc、ARF的异常表达可能对食管鳞状细胞癌的发生及发展有一定关系。因此,c-myc、TRIM24和ARF可能作为新的潜在治疗靶点,为食管鳞癌患者的个体化治疗提供新的方向。展开更多
[目的]探索CLIC4/ARF6信号通路对SiHa细胞增殖、侵袭能力的影响。[方法]用蛋白免疫印迹实验检测HUCEC细胞、SiHa细胞、C33A细胞以及HeLa细胞中CLIC4与ARF6的蛋白表达水平;将人宫颈癌SiHa细胞分为3组:si NC组、si CLIC4组与si ARF6组。通...[目的]探索CLIC4/ARF6信号通路对SiHa细胞增殖、侵袭能力的影响。[方法]用蛋白免疫印迹实验检测HUCEC细胞、SiHa细胞、C33A细胞以及HeLa细胞中CLIC4与ARF6的蛋白表达水平;将人宫颈癌SiHa细胞分为3组:si NC组、si CLIC4组与si ARF6组。通过CCK-8实验检测SiHa细胞的增殖活性;Transwell实验检测SiHa细胞的侵袭数量;通过TUNEL实验检测SiHa细胞的凋亡率。[结果]与HUCEC细胞相比,SiHa细胞、C33A细胞及HeLa细胞的CLIC4与ARF6的蛋白表达增加(0.13±0.02 vs 0.92±0.11 vs 0.89±0.08 vs 0.87±0.05;0.21±0.05 vs 0.72±0.06 vs 0.81±0.03 vs 0.77±0.03)。与si NC组比较,si CLIC4组及si ARF6组的SiHa细胞的增殖活性降低(P<0.05);si CLIC4组、si ARF6组的SiHa细胞侵袭数量减少(85.23±9.22 vs 36.62±7.75 vs 39.83±11.58);si CLIC4组、si ARF6组的SiHa细胞的凋亡率增加(5.73%±0.76%vs 26.58%±0.29%vs 29.03%±0.58%)。[结论]CLIC4与ARF6在宫颈癌细胞中表达上调,下调CLIC4或ARF6的表达后,宫颈癌SiHa细胞的增殖活性与侵袭数量降低。此外,下调CLIC4或ARF6的表达后,宫颈癌SiHa细胞凋亡率增加。展开更多
Although class A auxin response factors(ARFs)are known to regulate adventitious root(AR)development through the canonical SCFTIR1-Aux/IAA-ARF signaling pathway,the regulatory role of class B ARFs in AR development rem...Although class A auxin response factors(ARFs)are known to regulate adventitious root(AR)development through the canonical SCFTIR1-Aux/IAA-ARF signaling pathway,the regulatory role of class B ARFs in AR development remains largely unclear.Therefore,this research focused on the role of class B ARF transcription factors in peach(Prunus persica‘Shengli')adventitious root formation.Here,we report the role of a class B ARF gene Pp ARF4 in adventitious root formation in peach.Comparative transcriptome and q RT-PCR analyses showed that the transcription of Pp ARF4 was significantly up-regulated in auxin-treated stem explants.Y2H assay showed that Pp ARF4 had no interaction with Pp IAAs(AUXIN/INDOLE ACETIC ACIDs).Pp ARF4 could bind the promoters of lateral root development gene Pp LBD16 and auxin transport gene Pp PIN1 to activate their transcription.Ectopic overexpression of Pp ARF4 and Pp LBD16 in Arabidopsis promoted AR development.Additionally,Pp ARF4 could act as a negative regulator of flavone synthesis and thus prevent the explants from browning.The results not only provide novel insights into the functions of ARFs in regulating plant growth and development,but will also be useful for fulfilling asexual propagation by stem cuttings in peach.展开更多
Chemotherapeutic drugs such as doxorubicin(DOx)and oxaliplatin can induce immunogenic cell death(ICD)in tumor cells.Current studies have demonstrated that peptide-based drugs can also induce ICD in tumor cells.Unlike ...Chemotherapeutic drugs such as doxorubicin(DOx)and oxaliplatin can induce immunogenic cell death(ICD)in tumor cells.Current studies have demonstrated that peptide-based drugs can also induce ICD in tumor cells.Unlike small molecule drugs,peptide drugs not only enhance the effectiveness of immunotherapy but also offer the advantage of low biotoxicity[1].展开更多
Monocot root systems comprise a large number of lateral roots to allow them to survive and colonize land.Auxin signaling pathways centered on Aux/IAA play a crucial role in lateral root development.However,in non-mode...Monocot root systems comprise a large number of lateral roots to allow them to survive and colonize land.Auxin signaling pathways centered on Aux/IAA play a crucial role in lateral root development.However,in non-model monocot plants,the effects of Aux/IAA on lateral root initiation and number remain largely unknown.The present study transformed PheIAA17,a member of the Aux/IAA family of Moso bamboo,into rice and found that it significantly drove plants to produce lateral roots and improved the rooting rate.Quantitative experiments showed that PheIAA17 overexpression significantly affected the expression of ARF family members.Phylogenetic and promoter analyses indicate that PheARF3-2 belongs to class B ARF,and the promoter region contains auxin response elements.The results of yeast one-hybrid and dual-luciferase reporter assays confirmed that PheIAA17 bound specific fragments of the PheARF3-2 promoter to repress its transcriptional activity.Y2H and BiFC assay have shown that PheIAA17 and PheIAA30-3 could physically interact in vitro and in vivo.Taken together,this study reports a new molecular module centered on PheIAA17,which directs plants to alter root morphology through an increase in lateral roots.展开更多
文摘[目的]探索CLIC4/ARF6信号通路对SiHa细胞增殖、侵袭能力的影响。[方法]用蛋白免疫印迹实验检测HUCEC细胞、SiHa细胞、C33A细胞以及HeLa细胞中CLIC4与ARF6的蛋白表达水平;将人宫颈癌SiHa细胞分为3组:si NC组、si CLIC4组与si ARF6组。通过CCK-8实验检测SiHa细胞的增殖活性;Transwell实验检测SiHa细胞的侵袭数量;通过TUNEL实验检测SiHa细胞的凋亡率。[结果]与HUCEC细胞相比,SiHa细胞、C33A细胞及HeLa细胞的CLIC4与ARF6的蛋白表达增加(0.13±0.02 vs 0.92±0.11 vs 0.89±0.08 vs 0.87±0.05;0.21±0.05 vs 0.72±0.06 vs 0.81±0.03 vs 0.77±0.03)。与si NC组比较,si CLIC4组及si ARF6组的SiHa细胞的增殖活性降低(P<0.05);si CLIC4组、si ARF6组的SiHa细胞侵袭数量减少(85.23±9.22 vs 36.62±7.75 vs 39.83±11.58);si CLIC4组、si ARF6组的SiHa细胞的凋亡率增加(5.73%±0.76%vs 26.58%±0.29%vs 29.03%±0.58%)。[结论]CLIC4与ARF6在宫颈癌细胞中表达上调,下调CLIC4或ARF6的表达后,宫颈癌SiHa细胞的增殖活性与侵袭数量降低。此外,下调CLIC4或ARF6的表达后,宫颈癌SiHa细胞凋亡率增加。
基金supported by the National Natural Science Foundation of China(Grant Nos.32272690 and 32272687)the China Agriculture Research System(Grant No.CARS-30)Hubei Hongshan Laboratory(Grant No.2021hszd017)。
文摘Although class A auxin response factors(ARFs)are known to regulate adventitious root(AR)development through the canonical SCFTIR1-Aux/IAA-ARF signaling pathway,the regulatory role of class B ARFs in AR development remains largely unclear.Therefore,this research focused on the role of class B ARF transcription factors in peach(Prunus persica‘Shengli')adventitious root formation.Here,we report the role of a class B ARF gene Pp ARF4 in adventitious root formation in peach.Comparative transcriptome and q RT-PCR analyses showed that the transcription of Pp ARF4 was significantly up-regulated in auxin-treated stem explants.Y2H assay showed that Pp ARF4 had no interaction with Pp IAAs(AUXIN/INDOLE ACETIC ACIDs).Pp ARF4 could bind the promoters of lateral root development gene Pp LBD16 and auxin transport gene Pp PIN1 to activate their transcription.Ectopic overexpression of Pp ARF4 and Pp LBD16 in Arabidopsis promoted AR development.Additionally,Pp ARF4 could act as a negative regulator of flavone synthesis and thus prevent the explants from browning.The results not only provide novel insights into the functions of ARFs in regulating plant growth and development,but will also be useful for fulfilling asexual propagation by stem cuttings in peach.
基金supported by the National Natural Science Foundation of China(Grant Nos.:82073975,81673563,81102762,82204715,and 82304743).
文摘Chemotherapeutic drugs such as doxorubicin(DOx)and oxaliplatin can induce immunogenic cell death(ICD)in tumor cells.Current studies have demonstrated that peptide-based drugs can also induce ICD in tumor cells.Unlike small molecule drugs,peptide drugs not only enhance the effectiveness of immunotherapy but also offer the advantage of low biotoxicity[1].
基金supported by the National Key Research and Development Program of China(2021YFD2200505)the National Natural Science Foundation of China(32071849)。
文摘Monocot root systems comprise a large number of lateral roots to allow them to survive and colonize land.Auxin signaling pathways centered on Aux/IAA play a crucial role in lateral root development.However,in non-model monocot plants,the effects of Aux/IAA on lateral root initiation and number remain largely unknown.The present study transformed PheIAA17,a member of the Aux/IAA family of Moso bamboo,into rice and found that it significantly drove plants to produce lateral roots and improved the rooting rate.Quantitative experiments showed that PheIAA17 overexpression significantly affected the expression of ARF family members.Phylogenetic and promoter analyses indicate that PheARF3-2 belongs to class B ARF,and the promoter region contains auxin response elements.The results of yeast one-hybrid and dual-luciferase reporter assays confirmed that PheIAA17 bound specific fragments of the PheARF3-2 promoter to repress its transcriptional activity.Y2H and BiFC assay have shown that PheIAA17 and PheIAA30-3 could physically interact in vitro and in vivo.Taken together,this study reports a new molecular module centered on PheIAA17,which directs plants to alter root morphology through an increase in lateral roots.
