The Thermomyces lanuginosus lipase(TLLs)was successfully immobilized within a novel hydrogel matrix through a two-step crosslinking method.TLLs were initially crosslinked through the Schiff base reaction by oxidized c...The Thermomyces lanuginosus lipase(TLLs)was successfully immobilized within a novel hydrogel matrix through a two-step crosslinking method.TLLs were initially crosslinked through the Schiff base reaction by oxidized carboxymethyl cellulose(OCMC).The water-soluble OCMC@TLLs complex was subsequently crosslinked by carboxymethyl chitosan(CMCSH)in a microfluidic apparatus to form the CMCHS/OCMC@TLLs microspheres.The CD(Circular Dichroism,CD)and FT-IR(Fourier Transform infrared spectroscopy,FT-IR)spectra demonstrated that the crosslinking of TLLs with OCMC resulted in a less significant impact on their structure compared to that with glutaraldehyde.CMCHS/OCMC@TLLs showed decreased catalytic performance due to the mass transfer resistance,while its thermal stability was greatly improved.The CMCHS/OCMC@TLLs were used to catalyze the lauroylation of arbutin in tetrahydrofuran.After 12 h of reaction under optimal conditions,the yield of 6′-O-lauryl arbutin reached an impres-sive 92.12%.The prepared 6′-O-lauryl arbutin has high lipophilicity and exhibits similar tyrosinase inhibitory activity and higher antioxidant activity compared to its parent compound.展开更多
An antagonistic communication exists between adenosinergic and dopaminergic signaling in the basal ganglia,which suggests that the suppression of adenosine A2A receptors-cyclic adenosine monophosphate pathway may be a...An antagonistic communication exists between adenosinergic and dopaminergic signaling in the basal ganglia,which suggests that the suppression of adenosine A2A receptors-cyclic adenosine monophosphate pathway may be able to restore the disrupted dopamine transmission that results in motor symptoms in Parkinson’s disease(PD).Arbutin is a natural glycoside that possesses antioxidant,antiinflammatory,and neuroprotective properties.The purpose of this study was to investigate whether arbutin could ameliorate the symptoms of PD and to examine the underlying mechanism.In this study,Swiss albino mouse models of PD were established by the intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine for 4 successive days,with the concurrent intraperitoneal administration of arbutin(50 and 100 mg/kg)for 7 days.The results showed that arbutin significantly reduced lipid peroxidation,total nitrite levels,and inflammation in the substantia nigra and striatum of PD mouse models.In addition,arbutin decreased the activity of endogenous antioxidants,reduced the levels of dopamine,3,4-dihydroxyphenylacetic acid,homovanillic acid,andγ-aminobutyric acid,and minimized neurodegeneration in the striatum.Arbutin also reduced the abnormal performance of PD mouse models in the open field test,bar test,pole test,and rotarod test.The therapeutic efficacy of arbutin was similar to that of madopar.The intraperitoneal injection of the A2AR agonist CGS21680(0.5 mg/kg)attenuated the therapeutic effects of arbutin,whereas the intraperitoneal injection of forskolin(3 mg/kg)enhanced arbutin-mediated improvements.These findings suggest that arbutin can improve the performance of PD mouse models by inhibiting the function of the A2AR and enhancing the effects of cyclic adenosine monophosphate.This study was approved by the Institutional Animal Ethics Committee(1616/PO/Re/S/12/CPCSEA)on November 17,2019(approval No.IAEC/2019/010).展开更多
Heliciopsis lobata is a medicinal plant, which is exclusively used to treat tumor in Li folk region. Two new arbutin derivatives, 6'-((E)2-methoxy-5-hydroxycinnamoyl) arbutin(1) and 2'-((E)2, 5-dihydroxycinnam...Heliciopsis lobata is a medicinal plant, which is exclusively used to treat tumor in Li folk region. Two new arbutin derivatives, 6'-((E)2-methoxy-5-hydroxycinnamoyl) arbutin(1) and 2'-((E)2, 5-dihydroxycinnamoyl) arbutin(2) along with five known compounds(3–7), were isolated from the leaves of Heliciopsis lobata. Their structures were elucidated on the basis of extensive spectroscopic interpretations. They were evaluated for their potential anticancer activity. Compounds 6 and 7 exhibited cytotoxicity against MGC-803 cells with IC_(50) values being 44.1 and 11.3 μg·m L^(–1), respectively. Additionally, compounds 1, 2 and 5–7 exhibited a moderate inhibition of MGC-803 cells invasion; compound 2 at 20 μg·m L^(–1) inhibited the invasion of MGC-803 cells by 43.0%, compared with the controls..展开更多
Arbutin was synthesized from glucose by two-step reaction below: (a) 2,3,4,6-tetra-O-acetyl-α-D-glucosyl chloride or bromide was prepared by glucose and acetyl halide (chloride or bromide), (b) 2,3,4,6-tetra-O-ace-t...Arbutin was synthesized from glucose by two-step reaction below: (a) 2,3,4,6-tetra-O-acetyl-α-D-glucosyl chloride or bromide was prepared by glucose and acetyl halide (chloride or bromide), (b) 2,3,4,6-tetra-O-ace-tyl-α-D-glucosyl halide (Cl, Br) reacted with hydroquinone, methanol as solvent at pH=9.5-10.0.展开更多
The presence of excessive reactive oxygen species(Ros)at a skin wound site is an important factor affecting wound healing.ROS scavenging,which regulates the ROS microenvironment,is essential for wound healing.In this ...The presence of excessive reactive oxygen species(Ros)at a skin wound site is an important factor affecting wound healing.ROS scavenging,which regulates the ROS microenvironment,is essential for wound healing.In this study,we used novel electrospun PCL/gelatin/arbutin(PCL/G/A)nanofibrous membranes as wound dressings,with PCL/gelatin(PCL/G)as the backbone,and plantderived arbutin(hydroquinone-β-D-glucopyranoside,ARB)as an effective antioxidant that scavenges ROos and inhibits bacterial infection in wounds.The loading of ARB increased the mechanical strength of the nanofibres,with a water vapour transmission rate of more than 2500g/(m^(2)×24h),and the water contact angle decreased,indicating that hydrophilicity and air permeability were significantly improved.Drug release and degradation experiments showed that the nanofibre membrane controlled the drug release and exhibited favourable degradability.Haemolysis experiments showed that the PCL/G/A nanofibre membranes were biocompatible,and DPPH and ABTS+radical scavenging experiments indicated that PCL/G/A could effectively scavenge ROS to reflect the antioxidant activity.In addition,haemostasis experiments showed that PCL/G/A had good haemostatic effects in vitro and in vivo.In vivo animal wound closure and histological staining experiments demonstrated that PCL/G/A increased collagen deposition and remodelled epithelial tissue regeneration while showing good in vivo biocompatibility and non-toxicity.In conclusion,we successfully prepared a multifunctional wound dressing,PCL/G/A,for skin wound healing and investigated its potential role in wound healing,which is beneficial for the clinical translational application of phytomedicines.展开更多
为获得‘雀嘴茶’中乌金苷(6′-O-caffioylarbutin,CA)、绿原酸(3′-O-caffeoylquinic acid,CGA)和熊果苷(arbutin,Arb)3种主效成分高保留的干燥方法,该研究采用热风干燥(hot air drying,HAD)、真空冷冻干燥(freeze vacuum drying,FVD)...为获得‘雀嘴茶’中乌金苷(6′-O-caffioylarbutin,CA)、绿原酸(3′-O-caffeoylquinic acid,CGA)和熊果苷(arbutin,Arb)3种主效成分高保留的干燥方法,该研究采用热风干燥(hot air drying,HAD)、真空冷冻干燥(freeze vacuum drying,FVD)、真空干燥(vacuum drying,VD)、日晒干燥(sun drying,SD)、微波干燥(microware drying,MD)、自然阴干(natural air drying,NAD)、煮制阴干(boiling and natural air drying,B-NAD)、蒸制阴干(steaming and natural air drying,S-NAD)和烫制阴干(quick-boiling and natural air drying,QB-NAD)9种干燥方法对樟叶越桔叶芽进行干燥处理,并利用HPLC建立3种主效成分含量分析方法。进一步设置6个不同预处理时间对创新方法S-NAD、QB-NAD和B-NAD进行了优化试验。结果显示,9种干燥方法获得的3种主效成分含量均表现出CA>CGA>Arb。S-NAD是CA和CGA含量最高保留的干燥方法,分别高达428.726 mg/g和13.213 mg/g,VD是Arb含量最高保留的干燥方法,高达16.054 mg/g,S-NAD法也可得到较高的Arb含量(11.815 mg/g)。3种创新干燥方法的最佳预处理时间分别为S-NAD蒸制5 min、QB-NAD烫制和B-NAD煮制均为1 min。因此,干燥方法显著影响‘雀嘴茶’3种主效成分的保留含量,但3种主效成分含量高低顺序不受干燥方法的影响。为同时实现‘雀嘴茶’3种主效成分高保留,S-NAD是最适的干燥方法,且蒸制预处理5 min最佳。展开更多
The relative molecular mass of Arbutin is small. Both fluorolabeling and radiolabeling may affect its properties and functions. Therefore, the immunoassay of Arbutin was studied. Arbutin was coupled to bovine serum al...The relative molecular mass of Arbutin is small. Both fluorolabeling and radiolabeling may affect its properties and functions. Therefore, the immunoassay of Arbutin was studied. Arbutin was coupled to bovine serum albumin to get the Arbutin\|BSA conjugate with high molar ratio of Arbutin to BSA. Two rabbits were injected with the conjugate to develop the anti\|Arbutin serum. Ammonium sulfate precipitation and affinity chromatography were used to purify the antibody. Double agar diffusion test and enzyme\|linked immunosorbent assay (ELISA) were adopted to identify the antibody titer. The results demonstrated that the purity and activity of the antibody are high. The method proposed is satisfactory for the immunological detection of Arbutin.展开更多
目的传统β-熊果苷生产依赖植物提取与化学合成,存在原料供应不稳定、环境污染等问题。该研究旨在开发绿色高效的生物合成新路径。方法运用基因工程手段,构建糖基转移酶itUGT2与蔗糖合酶GmSuSy协同体系,实现UDPG的循环再生,建立β-熊果...目的传统β-熊果苷生产依赖植物提取与化学合成,存在原料供应不稳定、环境污染等问题。该研究旨在开发绿色高效的生物合成新路径。方法运用基因工程手段,构建糖基转移酶itUGT2与蔗糖合酶GmSuSy协同体系,实现UDPG的循环再生,建立β-熊果苷的酶法合成工艺。结果单酶催化的最适温度和pH分别为35℃与7.0。经优化,双酶体系最佳反应条件为:温度40℃、pH 6.5,itUGT2与GmSuSy酶量比1:2(20μg:40μg),底物HQ与蔗糖浓度比1:4。此外,添加0.5 mM MgSO_(4)或β-环糊精可显著提高产率,可能通过稳定酶结构、改善底物溶解性发挥作用。结论该研究成功开发的酶法合成技术,解决了传统工艺的弊端,实现辅因子原位再生,具有反应条件温和、绿色环保、易于产业化等优势,为β-熊果苷工业化生产提供了创新解决方案,具备显著的科学价值与应用潜力。展开更多
目的建立清喉咽颗粒(Qinghouyan Granules,QHY)多指标成分定量分析方法,结合化学计量学及熵权-优劣解距离法(technique for order preference by similarity to an ideal solution,TOPSIS)分析,对9个厂家的QHY质量进行综合评价,为其质...目的建立清喉咽颗粒(Qinghouyan Granules,QHY)多指标成分定量分析方法,结合化学计量学及熵权-优劣解距离法(technique for order preference by similarity to an ideal solution,TOPSIS)分析,对9个厂家的QHY质量进行综合评价,为其质量控制与评价提供方法及参考依据。方法HPLC法分析QHY中黄芩苷、连翘酯苷A、熊果苷、五福花苷酸、连翘酯苷I、连翘苷、汉黄芩苷、黄芩素、汉黄芩素、水苏糖、棉子糖11种成分含量,对9个厂家的177批次QHY样品进行定量测定;利用SIMCA 14.1和SPSS 25.0软件,对上述11种成分含量检测结果进行层次聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)和正交偏最小二乘法-判别分析(orthogonal partial least squaresdiscriminant analysis,OPLS-DA),筛选造成QHY质量差异的特征标志物;在PCA基础上设定Hotlling’s T2和DMod X控制限,对177批间样品进行质量差异的监控;运用熵权-TOPSIS模型对9个厂家QHY质量的优劣排序,多角度建立QHY质量综合评价方法。结果11种成分含量测定方法学考察结果精密度、重复性、稳定性及准确度的RSD值均小于3.0%。HCA和PCA结果均显示,不同厂家间及厂家内部不同批次间样品质量均有差异。OPLS-DA模型以VIP值>1为标准,筛选出水苏糖、棉子糖、汉黄芩素、黄芩苷、连翘酯苷I、连翘酯苷A 6个特征标志物,可能是影响QHY质量的主要成分。熵权-TOPSIS法结果显示,相对接近度C在0.084~0.850,表明不同厂家间QHY质量差异较大,厂家TCQ排序最优,厂家JM排序最后。结论建立的QHY中11种化学成分含量测定方法准确可靠,HCA、PCA和OPLS-DA可有效筛选影响QHY质量的特征标志物,熵权-TOPSIS法可综合评价QHY质量。展开更多
基金supported by the Youth Foundation of Southeast University ChengXian College(z0055).
文摘The Thermomyces lanuginosus lipase(TLLs)was successfully immobilized within a novel hydrogel matrix through a two-step crosslinking method.TLLs were initially crosslinked through the Schiff base reaction by oxidized carboxymethyl cellulose(OCMC).The water-soluble OCMC@TLLs complex was subsequently crosslinked by carboxymethyl chitosan(CMCSH)in a microfluidic apparatus to form the CMCHS/OCMC@TLLs microspheres.The CD(Circular Dichroism,CD)and FT-IR(Fourier Transform infrared spectroscopy,FT-IR)spectra demonstrated that the crosslinking of TLLs with OCMC resulted in a less significant impact on their structure compared to that with glutaraldehyde.CMCHS/OCMC@TLLs showed decreased catalytic performance due to the mass transfer resistance,while its thermal stability was greatly improved.The CMCHS/OCMC@TLLs were used to catalyze the lauroylation of arbutin in tetrahydrofuran.After 12 h of reaction under optimal conditions,the yield of 6′-O-lauryl arbutin reached an impres-sive 92.12%.The prepared 6′-O-lauryl arbutin has high lipophilicity and exhibits similar tyrosinase inhibitory activity and higher antioxidant activity compared to its parent compound.
文摘An antagonistic communication exists between adenosinergic and dopaminergic signaling in the basal ganglia,which suggests that the suppression of adenosine A2A receptors-cyclic adenosine monophosphate pathway may be able to restore the disrupted dopamine transmission that results in motor symptoms in Parkinson’s disease(PD).Arbutin is a natural glycoside that possesses antioxidant,antiinflammatory,and neuroprotective properties.The purpose of this study was to investigate whether arbutin could ameliorate the symptoms of PD and to examine the underlying mechanism.In this study,Swiss albino mouse models of PD were established by the intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine for 4 successive days,with the concurrent intraperitoneal administration of arbutin(50 and 100 mg/kg)for 7 days.The results showed that arbutin significantly reduced lipid peroxidation,total nitrite levels,and inflammation in the substantia nigra and striatum of PD mouse models.In addition,arbutin decreased the activity of endogenous antioxidants,reduced the levels of dopamine,3,4-dihydroxyphenylacetic acid,homovanillic acid,andγ-aminobutyric acid,and minimized neurodegeneration in the striatum.Arbutin also reduced the abnormal performance of PD mouse models in the open field test,bar test,pole test,and rotarod test.The therapeutic efficacy of arbutin was similar to that of madopar.The intraperitoneal injection of the A2AR agonist CGS21680(0.5 mg/kg)attenuated the therapeutic effects of arbutin,whereas the intraperitoneal injection of forskolin(3 mg/kg)enhanced arbutin-mediated improvements.These findings suggest that arbutin can improve the performance of PD mouse models by inhibiting the function of the A2AR and enhancing the effects of cyclic adenosine monophosphate.This study was approved by the Institutional Animal Ethics Committee(1616/PO/Re/S/12/CPCSEA)on November 17,2019(approval No.IAEC/2019/010).
基金supported by Hainan Province modernization of Chinese medicine program,China(No.24201326)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Heliciopsis lobata is a medicinal plant, which is exclusively used to treat tumor in Li folk region. Two new arbutin derivatives, 6'-((E)2-methoxy-5-hydroxycinnamoyl) arbutin(1) and 2'-((E)2, 5-dihydroxycinnamoyl) arbutin(2) along with five known compounds(3–7), were isolated from the leaves of Heliciopsis lobata. Their structures were elucidated on the basis of extensive spectroscopic interpretations. They were evaluated for their potential anticancer activity. Compounds 6 and 7 exhibited cytotoxicity against MGC-803 cells with IC_(50) values being 44.1 and 11.3 μg·m L^(–1), respectively. Additionally, compounds 1, 2 and 5–7 exhibited a moderate inhibition of MGC-803 cells invasion; compound 2 at 20 μg·m L^(–1) inhibited the invasion of MGC-803 cells by 43.0%, compared with the controls..
文摘Arbutin was synthesized from glucose by two-step reaction below: (a) 2,3,4,6-tetra-O-acetyl-α-D-glucosyl chloride or bromide was prepared by glucose and acetyl halide (chloride or bromide), (b) 2,3,4,6-tetra-O-ace-tyl-α-D-glucosyl halide (Cl, Br) reacted with hydroquinone, methanol as solvent at pH=9.5-10.0.
基金the Guangxi Science and Technology Base andTalent Special Project(Grant No.GuikeAD19254003)Guangxi Scientific Research and Technological Development Foundation(Grant No.GuikeAB21220062)+4 种基金Guangxi University's young and middle-aged teachers'basic scientific research ability enhancement project(Grant No.2022KY0104)Open Research Fund From Guangxi Key Laboratory Of Regenerative Medicine,Guangxi Medical University(Grant No.202003)The Foundation of the Collaborative Innovation Centre of Regenerative Medicine and Medical Bioresource Development and Application Coconstructed by the Province and Ministry(Grant No.CICRMMBRTC-2023001)‘Medical Excellence Award'Funded By The Creative Research Development Grant From The First Affiliated Hospital of Guangxi Medical University(Grant No.202210)Guangxi Health Commission self-funded scientific research project(Grant No.Z20180949).
文摘The presence of excessive reactive oxygen species(Ros)at a skin wound site is an important factor affecting wound healing.ROS scavenging,which regulates the ROS microenvironment,is essential for wound healing.In this study,we used novel electrospun PCL/gelatin/arbutin(PCL/G/A)nanofibrous membranes as wound dressings,with PCL/gelatin(PCL/G)as the backbone,and plantderived arbutin(hydroquinone-β-D-glucopyranoside,ARB)as an effective antioxidant that scavenges ROos and inhibits bacterial infection in wounds.The loading of ARB increased the mechanical strength of the nanofibres,with a water vapour transmission rate of more than 2500g/(m^(2)×24h),and the water contact angle decreased,indicating that hydrophilicity and air permeability were significantly improved.Drug release and degradation experiments showed that the nanofibre membrane controlled the drug release and exhibited favourable degradability.Haemolysis experiments showed that the PCL/G/A nanofibre membranes were biocompatible,and DPPH and ABTS+radical scavenging experiments indicated that PCL/G/A could effectively scavenge ROS to reflect the antioxidant activity.In addition,haemostasis experiments showed that PCL/G/A had good haemostatic effects in vitro and in vivo.In vivo animal wound closure and histological staining experiments demonstrated that PCL/G/A increased collagen deposition and remodelled epithelial tissue regeneration while showing good in vivo biocompatibility and non-toxicity.In conclusion,we successfully prepared a multifunctional wound dressing,PCL/G/A,for skin wound healing and investigated its potential role in wound healing,which is beneficial for the clinical translational application of phytomedicines.
文摘为获得‘雀嘴茶’中乌金苷(6′-O-caffioylarbutin,CA)、绿原酸(3′-O-caffeoylquinic acid,CGA)和熊果苷(arbutin,Arb)3种主效成分高保留的干燥方法,该研究采用热风干燥(hot air drying,HAD)、真空冷冻干燥(freeze vacuum drying,FVD)、真空干燥(vacuum drying,VD)、日晒干燥(sun drying,SD)、微波干燥(microware drying,MD)、自然阴干(natural air drying,NAD)、煮制阴干(boiling and natural air drying,B-NAD)、蒸制阴干(steaming and natural air drying,S-NAD)和烫制阴干(quick-boiling and natural air drying,QB-NAD)9种干燥方法对樟叶越桔叶芽进行干燥处理,并利用HPLC建立3种主效成分含量分析方法。进一步设置6个不同预处理时间对创新方法S-NAD、QB-NAD和B-NAD进行了优化试验。结果显示,9种干燥方法获得的3种主效成分含量均表现出CA>CGA>Arb。S-NAD是CA和CGA含量最高保留的干燥方法,分别高达428.726 mg/g和13.213 mg/g,VD是Arb含量最高保留的干燥方法,高达16.054 mg/g,S-NAD法也可得到较高的Arb含量(11.815 mg/g)。3种创新干燥方法的最佳预处理时间分别为S-NAD蒸制5 min、QB-NAD烫制和B-NAD煮制均为1 min。因此,干燥方法显著影响‘雀嘴茶’3种主效成分的保留含量,但3种主效成分含量高低顺序不受干燥方法的影响。为同时实现‘雀嘴茶’3种主效成分高保留,S-NAD是最适的干燥方法,且蒸制预处理5 min最佳。
文摘The relative molecular mass of Arbutin is small. Both fluorolabeling and radiolabeling may affect its properties and functions. Therefore, the immunoassay of Arbutin was studied. Arbutin was coupled to bovine serum albumin to get the Arbutin\|BSA conjugate with high molar ratio of Arbutin to BSA. Two rabbits were injected with the conjugate to develop the anti\|Arbutin serum. Ammonium sulfate precipitation and affinity chromatography were used to purify the antibody. Double agar diffusion test and enzyme\|linked immunosorbent assay (ELISA) were adopted to identify the antibody titer. The results demonstrated that the purity and activity of the antibody are high. The method proposed is satisfactory for the immunological detection of Arbutin.
文摘目的传统β-熊果苷生产依赖植物提取与化学合成,存在原料供应不稳定、环境污染等问题。该研究旨在开发绿色高效的生物合成新路径。方法运用基因工程手段,构建糖基转移酶itUGT2与蔗糖合酶GmSuSy协同体系,实现UDPG的循环再生,建立β-熊果苷的酶法合成工艺。结果单酶催化的最适温度和pH分别为35℃与7.0。经优化,双酶体系最佳反应条件为:温度40℃、pH 6.5,itUGT2与GmSuSy酶量比1:2(20μg:40μg),底物HQ与蔗糖浓度比1:4。此外,添加0.5 mM MgSO_(4)或β-环糊精可显著提高产率,可能通过稳定酶结构、改善底物溶解性发挥作用。结论该研究成功开发的酶法合成技术,解决了传统工艺的弊端,实现辅因子原位再生,具有反应条件温和、绿色环保、易于产业化等优势,为β-熊果苷工业化生产提供了创新解决方案,具备显著的科学价值与应用潜力。
文摘目的建立清喉咽颗粒(Qinghouyan Granules,QHY)多指标成分定量分析方法,结合化学计量学及熵权-优劣解距离法(technique for order preference by similarity to an ideal solution,TOPSIS)分析,对9个厂家的QHY质量进行综合评价,为其质量控制与评价提供方法及参考依据。方法HPLC法分析QHY中黄芩苷、连翘酯苷A、熊果苷、五福花苷酸、连翘酯苷I、连翘苷、汉黄芩苷、黄芩素、汉黄芩素、水苏糖、棉子糖11种成分含量,对9个厂家的177批次QHY样品进行定量测定;利用SIMCA 14.1和SPSS 25.0软件,对上述11种成分含量检测结果进行层次聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)和正交偏最小二乘法-判别分析(orthogonal partial least squaresdiscriminant analysis,OPLS-DA),筛选造成QHY质量差异的特征标志物;在PCA基础上设定Hotlling’s T2和DMod X控制限,对177批间样品进行质量差异的监控;运用熵权-TOPSIS模型对9个厂家QHY质量的优劣排序,多角度建立QHY质量综合评价方法。结果11种成分含量测定方法学考察结果精密度、重复性、稳定性及准确度的RSD值均小于3.0%。HCA和PCA结果均显示,不同厂家间及厂家内部不同批次间样品质量均有差异。OPLS-DA模型以VIP值>1为标准,筛选出水苏糖、棉子糖、汉黄芩素、黄芩苷、连翘酯苷I、连翘酯苷A 6个特征标志物,可能是影响QHY质量的主要成分。熵权-TOPSIS法结果显示,相对接近度C在0.084~0.850,表明不同厂家间QHY质量差异较大,厂家TCQ排序最优,厂家JM排序最后。结论建立的QHY中11种化学成分含量测定方法准确可靠,HCA、PCA和OPLS-DA可有效筛选影响QHY质量的特征标志物,熵权-TOPSIS法可综合评价QHY质量。
